FastQCFastQC Report
Mon 20 Jul 2015
000000000-AH092 l01n01.3310000000e71b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AH092 l01n01.3310000000e71b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16310244
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACTGGAGATATGTT8854515.42880290448138No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCTTTGGGGA8270605.070800902794588No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATCTGAGCA4131092.5328192515084385No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTTTGGCA3461492.1222797157418367No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTCTTCGGACTGGCCGGCGCAG2891391.7727447854244243No Hit
GCTCCTACCGATGGAACGCAGAGGCAAATTAGGTGGAGACTCGCTCCGGT2569751.5755435663623425No Hit
GCTACTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACTCGAGTC2563441.5716748320871228No Hit
GCTACTACCGATTGGAAGATTCGATGAGAACTTTGGATTGGGCGCGAAGT2261781.3867235830438833No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGCTGTCGG1785071.0944471462229504No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTCAGGC1580920.9692804105199161No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTCTCGTA1468100.9001091583914992No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTCTTCAGACTGGCCGGCGCAG1458370.8941435824013424No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTTTTCGGACTGGCCGGCGCAG1444590.885694904380339No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTGGATC1334880.8184304293669672No Hit
GCTACTACCGATTGAACGGCTTAGTGAGGCTTTCGGATTGGCCCGGGGAG1145170.7021170253492223No Hit
GCTACTACCGATTGAATGGTTTAGTGAGATCATCTGAGTAGATGTTCATG1114850.6835274812565649No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATTTTGGCA1029150.6309838160606304No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTA980790.6013337384774868No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG924850.5670362748711791No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACCGGAAATATGTT875610.5368466590689877No Hit
GCTCCTACCGATTGAATGATCCGGTGAAATCTTGGGATCGAGAGGCACAC727890.4462778116624129No Hit
GCTACTACCGATCGAGTGATTTAGTGAGGCCTTTGGACTGGTCTTCGTTA588060.3605464148788945No Hit
GCTACTACCGATTGGATGACTTAGTGAGGTCTTAGGACCGAGGTAAAGGT582050.356861614087441No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTCTTCGGACTGGCCGGTGCAG564840.3463099632353753No Hit
GCTTCAACCGATTGGATGATCCGGCAAAGTGGATGGACAAGGTGCTTGCC513830.3150351398789619No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGTG512770.31438524156965403No Hit
GCTACTACCGATTGAATGATTTAGTGAGATCTTCGGACTGGCGATCGCGG508590.31182243502917556No Hit
GCTCCTACCGATGGGACGAGAAGGCAAATTTGATGGACTGGGTCTGCTAA508080.3115097481067727No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTTTGGATCTGCCATTAGGT482130.29559950176097916No Hit
GCTCCTACCGATTGAATGTTCCGGTGAATCCTTCGGACCTATTTCTAGAG466180.2858203715407323No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTAGCTG452300.2773103823584736No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTGCTGA428820.26291452169569013No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGTTTGGAC420320.2577030729889755No Hit
GCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATCGCGGCGACGTG415880.2549808574292328No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGAGGG400930.24581483882154062No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACTGCGCGGGGC395320.242375282675109No Hit
GCTTCTACCGATTGGGCGTCCCGGTGAACAATCGGGAGGCCGCGCTCATG389920.2390644799673138No Hit
GCTCCTACCGATGGTTGAACCGATGAGGACTCGGGATGATCTTATTAGAT386740.23711478503938996No Hit
GCTCCTACCGATTGAATGATCCGGTGAAATCTTCGGATGGTGGCATATAG375970.23051157297217625No Hit
AAACCAACCGAGTTATGAAGGGATGAAGCGATTTTTCGAAAAATCCTTTT373160.22878872934089767No Hit
GCTACTACCGATTGGATGGCTTAGTGAGGCCCTCGGATCGTCGGCTTGCT367610.2253859598912193No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGTCCAGAGAG365750.22424557229186762No Hit
GCTTCACCGAAGCTGGTTCTTGGCGAGGTGGCGCCTAATTGGCGCTATCG356360.21848845424997937No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTTCAGGGATTGTTGCGCTTGG354550.21737872223125543No Hit
GCTCCTACCGATGGAACGCAGAGGCAAATTAGGTGGAGACTCGCTTCGGT348830.21387172380744274No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTCAGGATCGTCCAGTGGAG341840.20958607363568563No Hit
GCCTCAATCGACTAGATGATCCGGTGAATGATTCGGACTGGAGATATGTT339400.20809008130105228No Hit
GTTATTTCCGATCGAATGGCTCGGTGAGGGGGTAGGAGCTCCGCGCGCGC320810.1966923364236611No Hit
GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACTGTGAACCTGTT312320.19148701883307204No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGATATGGAC311350.190892300568894No Hit
GCTACTACCGATTGGAAGTAGACGAGAGGTCTCGGGATCGATTCCTGGGC310990.1906715803883743No Hit
GCTACTACCGATTGAATGGCTTAGTGAGTTCCAGGGATTGTTGTCCTTGT310760.19053056471748675No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACTGGCGGTCGCGG305370.1872258931258171No Hit
GCTACTACCGATTGAATGGCTTAGTGAGACCTCCGGATCGGGCTCTACTG301140.18463243100471088No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTTCGGATTGGCTTTGGGGA295040.18089245016812747No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATGTTG285050.1747674651587064No Hit
GCTTCTACCGATTGGTCGTCCCGGTGAACAATCGGGAGGCCGTGCTCATG260000.1594090192642121No Hit
AAACCATGCGAGTAGGCTCCAAGTGAGGGTCCGATTCCTGTCGAATTCGA241610.14813389670933189No Hit
GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG239580.1468892801358459No Hit
GCACCTACCGATTGAATGATTCGGTGAAGATTTCGGACCGTGGTCGATCG236650.14509286311106076No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGAACTAGGCA235450.14435712917599516No Hit
GCTGCCCGGAACTGAGCCGTTTCGAGAAAAATGGGGACTGTCTACTTGAG228400.1400346923074848No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAATCCTCGGAGCCGTGGCCTCTA226420.13882073131462658No Hit
GCTACTACCGATCGAGTGATTTAGTGAGGCCTTTGGACTGGTCTCCGTTA222870.13664418509005752No Hit
GCTCCTACCGATTGGATGACTCGGTGAAATCACCGGATGCTTACGAGCAG217010.1330513510404872No Hit
GCTACTACCGATCGAGTGATTTAGTGAGGACTTTGGACTGGTCTTCGATG215900.13237079715055153No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATTCTGGCA214030.13122427843507428No Hit
AAACCATGCGAGTGGGGTCGTAGTGAAGGCACGCTTCTTGGCGTGTTTGA213450.13086867369979258No Hit
GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACTGTTGACTCGTT212890.130525331196762No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGTTCACGGACTGTTGCCTCTGG207420.12717161067608798No Hit
GCTTCAACCGATTGGATGATCCGGCAAAGTGGATGGACAAGGTTCTTGCC206490.12660141687641216No Hit
GCTGCTACCGATTGAATGGATTAGTGAGCTTTATAGACCGCTGTATTTGC205070.12573079838658455No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTTGGGATTCATACTACTCA200670.12303310729134401No Hit
GCTACTACCGATTGAATGGCTTAGTGAGTTTCAGGGATTGTTGTATTTGG197780.12126121473106104No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCCTCGGATAGCCGGCTGCCA193780.1188087682808424No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTTCGGACTGGCCCAGGGAG190660.11689586004967184No Hit
TTCTGCTTGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTCAGGCG190170.11659543535952008No Hit
GCTACTACCGATTGAATGGCTCGGTGAGGCCTCCGGACTGGCTCAGGAGG188120.11533855655378301No Hit
GCTCCTACCGATTGAATGGTTCGGTGAAATCTCCGGATAGTGGTACATGG187990.11525885204415091No Hit
GTTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGTGCCTTTTCA186230.1141797756060547No Hit
GCTGCCCGGAACTGAGCCGTTTCGAGAAACATGGGGACTGTCTACTTGAG181520.11129201991092225No Hit
GCTACTACCGATTGAATGGTTTAGTGAAAACTCGGGACCGGGCATTTATA180090.11041527030496907No Hit
GCTGCAACCGATTGGGTGCCTCGGTGAATTGTCGGGAGAACTTGGCTGGC177150.10861272216405836No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCCCTCCGGATTGGGATCTCGGC171040.10486661021134938No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTTCGGATTGGCTTCGGAGG171030.10486047909522385No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACTGTGGTCTGGAC169760.10408182734727942No Hit
GCTTCTACCGATTGGACGTCCCGGTGAACAATCGGGAGGCCGTACTCATG168620.10338288010896711No Hit
GCTACTACCGATTGAATGATTCAGTGAGGCTTTCGGACTGGCCCAGGAAG168230.10314376658007078No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCGAGGAG164030.10056869780734119No Hit
GCTCCTACCGATTGGAAGTTTCGGTGAGAACATTGGAGAGCGTCGCTTTA163730.10038476432357481No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGACTTTGGGAA163710.10037250209132371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACC7756550.094.973673
ACTACCG7761500.094.957164
CTACCGA11273650.094.9047855
GCTACTA7696050.094.88891
TACCGAT11317350.094.8176
ACCGATT10512500.094.7663967
CTACTAC7718850.094.6886142
CCGATTG9863200.094.663378
CCTACCG3079600.094.651414
GCTCCTA2686900.094.619921
TCAATCG1123750.094.6195764
CGATTGA8226200.094.570989
AATCGAC1119950.094.44446
TCCTACC2669100.094.200943
CCACGAA285500.094.168134
ATCGACT1125200.093.965747
TCTACCG168050.093.728064
CCGGGAC279800.093.641896
CTCCTAC2723300.093.49652
ACCGATC292850.093.4915547
Produced by FastQC (version 0.11.2)