##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AGWYV l01n01 ss.3410000000ebd0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 701371 Sequences flagged as poor quality 0 Sequence length 351 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24034783302988 33.0 32.0 34.0 32.0 34.0 2 32.57476285731802 33.0 32.0 34.0 32.0 34.0 3 32.47108449023413 33.0 32.0 34.0 32.0 34.0 4 32.37741081396294 33.0 32.0 34.0 32.0 34.0 5 32.6568834468491 33.0 32.0 34.0 32.0 34.0 6 36.540220510970656 37.0 37.0 37.0 37.0 37.0 7 36.870178550296494 37.0 37.0 37.0 37.0 37.0 8 36.27409174317159 37.0 37.0 37.0 37.0 37.0 9 35.859073158143126 37.0 37.0 37.0 33.0 37.0 10-19 36.15717230966207 37.4 36.5 37.7 33.8 37.7 20-29 36.96610424440132 38.2 37.8 38.7 34.4 38.7 30-39 36.98534056868618 38.0 37.4 39.0 34.0 39.0 40-49 37.034925167992405 38.2 37.6 39.0 34.6 39.0 50-59 37.00286610082254 38.4 37.7 39.0 34.7 39.0 60-69 36.85985149086575 38.0 37.0 39.0 33.9 39.0 70-79 36.79916178456195 38.0 37.0 39.0 33.9 39.0 80-89 36.87453302175311 38.1 37.3 39.0 34.1 39.0 90-99 36.76544353844114 38.4 37.4 39.0 33.6 39.0 100-109 36.69902676329646 38.1 37.1 39.0 33.1 39.0 110-119 36.53414098957613 38.0 37.0 39.0 32.9 39.0 120-129 36.40174928817986 38.5 37.0 39.0 32.2 39.0 130-139 35.95760617419312 38.0 37.0 39.0 29.6 39.0 140-149 35.03622890025393 38.0 35.7 39.0 26.0 39.0 150-159 34.779726136381456 38.0 35.1 39.0 18.7 39.0 160-169 34.21820962087112 38.0 34.4 39.0 15.2 39.0 170-179 32.98872793428869 37.6 31.5 38.5 14.9 39.0 180-189 32.31437627161658 37.0 30.0 38.0 14.9 39.0 190-199 31.356425201498205 37.0 25.7 38.0 14.0 38.0 200-209 29.886411186091244 36.4 21.4 37.1 13.8 38.0 210-219 28.977678717825512 35.7 15.0 37.0 13.7 37.8 220-229 28.286465650846697 34.1 14.8 37.0 13.6 37.0 230-239 27.31451884951046 32.5 14.0 37.0 13.0 37.0 240-249 26.505591477263813 30.2 14.0 37.0 13.0 37.0 250-259 25.6310306528214 26.0 14.0 37.0 13.0 37.0 260-269 24.82242465114754 25.1 14.0 37.0 13.0 37.0 270-279 23.922803338033646 24.1 14.0 36.1 13.0 37.0 280-289 23.468817786877416 24.0 14.0 35.6 13.0 37.0 290-299 22.918896133430092 18.2 14.0 33.8 13.0 37.0 300-309 21.99176284733757 15.0 14.0 32.7 13.0 37.0 310-319 21.013071256154014 15.0 14.0 27.1 13.0 36.9 320-329 20.49378916436522 15.0 14.0 26.0 13.0 36.1 330-339 19.44403689345582 14.1 14.0 25.2 13.0 33.1 340-349 18.989887092565844 14.0 14.0 25.0 13.0 29.5 350-351 18.464194413512963 14.0 14.0 24.5 13.0 27.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2916433875897866 1101 2 0.09643563972458935 1101 3 0.45918929241621953 1101 4 0.2788049543133013 1101 5 0.3534048909411851 1101 6 0.15422127379550687 1101 7 0.04060160599438234 1101 8 0.12075007349832845 1101 9 0.03182711336988575 1101 10-19 0.11177148881733956 1101 20-29 0.1374833820615251 1101 30-39 0.12327762168823142 1101 40-49 0.16931296229898152 1101 50-59 0.18007287377810144 1101 60-69 0.1445012238283212 1101 70-79 0.25570941455330143 1101 80-89 0.10695843003941974 1101 90-99 0.19456888524571525 1101 100-109 0.27094401235936516 1101 110-119 0.19004534483696034 1101 120-129 0.11719228344114185 1101 130-139 0.08291050439422776 1101 140-149 0.5333912436099553 1101 150-159 0.392213115007479 1101 160-169 0.24021468160128023 1101 170-179 0.5325857298187628 1101 180-189 -0.03376467222761903 1101 190-199 0.12810851763395092 1101 200-209 0.2274173285660268 1101 210-219 0.17035294132580248 1101 220-229 0.1490391055740865 1101 230-239 0.20268409782162777 1101 240-249 0.29160137541028064 1101 250-259 0.17988969419743128 1101 260-269 0.22469725621459347 1101 270-279 0.13042310883298214 1101 280-289 0.1814403055191285 1101 290-299 0.18323912906134154 1101 300-309 0.3253944624053844 1101 310-319 0.3760732445755828 1101 320-329 0.19577214668116838 1101 330-339 0.24427557390662002 1101 340-349 0.05057340318975889 1101 350-351 0.01136248097209247 1102 1 -0.22050941978395144 1102 2 -0.09283809417642885 1102 3 -0.6459728168253775 1102 4 -0.23472881465067275 1102 5 -0.22378388739567612 1102 6 0.008640951550262344 1102 7 -0.05087115191186342 1102 8 0.004114902775135931 1102 9 0.015010746104415773 1102 10-19 0.0831569308610085 1102 20-29 0.040432199256031254 1102 30-39 0.07422122877063231 1102 40-49 0.06808293186554693 1102 50-59 0.05323943039869761 1102 60-69 0.12358932139745349 1102 70-79 0.12909414698957278 1102 80-89 -0.038295055385169974 1102 90-99 0.02651231777228702 1102 100-109 -0.09854854245494948 1102 110-119 -0.8612541664067024 1102 120-129 -0.16515930615533136 1102 130-139 -0.12436669026588021 1102 140-149 0.48345112384946987 1102 150-159 0.21367004904814024 1102 160-169 -0.01968481598149907 1102 170-179 0.2682915425279404 1102 180-189 -2.0035599317225206E-4 1102 190-199 0.16495091648642912 1102 200-209 0.4125361403720724 1102 210-219 -0.17805605816570846 1102 220-229 -0.043705177722440425 1102 230-239 0.19001453201028085 1102 240-249 0.4758251521913195 1102 250-259 0.23783414749615517 1102 260-269 0.4014397598005779 1102 270-279 0.3181186493109607 1102 280-289 0.556395300472186 1102 290-299 0.44034611281247393 1102 300-309 0.43685961195419765 1102 310-319 0.8081746691029572 1102 320-329 0.1248999746753654 1102 330-339 0.15571987162297773 1102 340-349 0.41448745721099556 1102 350-351 -0.03321575798725007 1103 1 -0.1659808852453537 1103 2 -0.02898685371480525 1103 3 -0.3280876433989377 1103 4 -0.2332641084778473 1103 5 0.07339424759838664 1103 6 0.09824872691066844 1103 7 0.08741432128191207 1103 8 0.1700340875938977 1103 9 0.18508332058875254 1103 10-19 0.15902202975227908 1103 20-29 -0.13738901218741262 1103 30-39 -0.07951166604647852 1103 40-49 -0.26366985739394977 1103 50-59 -0.952110414343899 1103 60-69 -0.024752735784907998 1103 70-79 -0.24587749565989014 1103 80-89 -0.6984375465330288 1103 90-99 -0.05920302090378726 1103 100-109 -0.22064901243900437 1103 110-119 -0.38899705927764927 1103 120-129 -0.21050227396810328 1103 130-139 -0.10104750777089322 1103 140-149 0.5954068152169398 1103 150-159 0.07205845107331044 1103 160-169 -0.11756730980170715 1103 170-179 0.29784091844837945 1103 180-189 -0.04749903695881841 1103 190-199 -0.2981825671178626 1103 200-209 -0.07872427668794302 1103 210-219 -0.08077683883884745 1103 220-229 -0.23554675707799433 1103 230-239 -0.3309701978650743 1103 240-249 -0.2341117209333312 1103 250-259 -0.37186290450127757 1103 260-269 -0.4277315302156026 1103 270-279 -0.4006734146975006 1103 280-289 -0.46698589784191213 1103 290-299 -0.16332236978969306 1103 300-309 -0.03904067038480008 1103 310-319 0.024083396921774636 1103 320-329 -0.08695390617873855 1103 330-339 0.09692517157911595 1103 340-349 0.17542028696231782 1103 350-351 -0.17193494251553787 2112 1 -0.241451491310535 2112 2 0.08376439334840313 2112 3 0.37104170966430416 2112 4 0.44005086464703425 2112 5 0.19371565182516548 2112 6 0.09404073720262573 2112 7 0.07692321770811361 2112 8 0.11736008507302387 2112 9 0.20536083587101928 2112 10-19 0.03157109964953975 2112 20-29 0.09183313856288322 2112 30-39 -0.022248059445026058 2112 40-49 -0.02800206653025583 2112 50-59 -0.07917638354208378 2112 60-69 -0.11363170241971687 2112 70-79 -0.7851048993682284 2112 80-89 -0.734346177979738 2112 90-99 -0.1871815098348648 2112 100-109 -0.15901309317820989 2112 110-119 0.057025710855377554 2112 120-129 -0.29543166077097993 2112 130-139 -0.12908066586847866 2112 140-149 0.44528236262865306 2112 150-159 -0.8771387378397861 2112 160-169 -0.22051778524241428 2112 170-179 0.2963408766167319 2112 180-189 -0.2504432719402203 2112 190-199 0.019788927012672985 2112 200-209 0.1690431612812091 2112 210-219 0.018952188074678133 2112 220-229 -0.15894621642106088 2112 230-239 0.002458982522604458 2112 240-249 0.007810751989257625 2112 250-259 -0.1783083800311296 2112 260-269 -1.124321976415299 2112 270-279 -0.008601678217694086 2112 280-289 -0.02596836009399084 2112 290-299 0.08653128801094567 2112 300-309 0.12900505092314418 2112 310-319 -0.13633468642058588 2112 320-329 0.20998035504003454 2112 330-339 0.24283092281383745 2112 340-349 0.22926729010174896 2112 350-351 0.34948121657091136 1113 1 0.03565246318653692 1113 2 -0.13188765468952823 1113 3 0.004106537571921365 1113 4 -0.1399475112570343 1113 5 0.14483972039732151 1113 6 -0.038822137906166176 1113 7 -0.010036351129720344 1113 8 0.11360153870950285 1113 9 0.0913381797327375 1113 10-19 -0.07444557557893461 1113 20-29 0.13159021264813475 1113 30-39 0.035400337907702806 1113 40-49 0.0734514110651574 1113 50-59 0.13233183667825443 1113 60-69 0.17929788419491643 1113 70-79 0.22366341805072665 1113 80-89 -0.11186162895762664 1113 90-99 0.12935388751255772 1113 100-109 0.17309263546714249 1113 110-119 0.19717698126910932 1113 120-129 0.24670146566666773 1113 130-139 0.34700226188220284 1113 140-149 0.383205302807184 1113 150-159 -1.3457752739962316 1113 160-169 0.16926160250871902 1113 170-179 0.46148715999675716 1113 180-189 0.2955774105684412 1113 190-199 0.6313823240035106 1113 200-209 0.6053342381097657 1113 210-219 0.5613855074619671 1113 220-229 0.2955857173652561 1113 230-239 0.25222931417358296 1113 240-249 0.3257685572707487 1113 250-259 0.32758415777764327 1113 260-269 -0.20448373283974774 1113 270-279 0.5886863463697054 1113 280-289 0.6197213246019047 1113 290-299 0.5480060553856845 1113 300-309 0.4220802059625157 1113 310-319 0.3631088488832006 1113 320-329 0.45836678042253354 1113 330-339 0.4172154169344324 1113 340-349 0.4339483053029376 1113 350-351 0.40236160367064855 2113 1 0.04905593043467604 2113 2 0.18940035676262568 2113 3 0.30598967023464496 2113 4 0.007951758599077152 2113 5 0.18725842509932278 2113 6 0.008897293016758567 2113 7 0.07453934558723319 2113 8 0.020123251781583917 2113 9 0.3095711450700591 2113 10-19 -0.06834663474343472 2113 20-29 0.1700011171860325 2113 30-39 -0.019213251755139993 2113 40-49 0.022000022607620906 2113 50-59 0.0762385623492392 2113 60-69 0.0986457165200747 2113 70-79 0.12564963754285685 2113 80-89 0.15894463863442354 2113 90-99 0.039647623225967266 2113 100-109 -0.03311262501236456 2113 110-119 0.11647806491583168 2113 120-129 0.17201384091725203 2113 130-139 0.16808587953975973 2113 140-149 0.2461773890877481 2113 150-159 -0.406798009400525 2113 160-169 -0.20979331865237327 2113 170-179 0.0320832401545772 2113 180-189 -0.20723145126650877 2113 190-199 -0.2694205763760422 2113 200-209 -0.2488072153273464 2113 210-219 -0.2560230175064433 2113 220-229 -0.3154781546966525 2113 230-239 -0.4925822000222908 2113 240-249 -0.20209445420865535 2113 250-259 -0.11240474168146264 2113 260-269 -0.301327797482962 2113 270-279 0.005756933241791273 2113 280-289 -0.07045569560754217 2113 290-299 -0.012115414898392629 2113 300-309 -0.1468705531858916 2113 310-319 0.023139673225493596 2113 320-329 0.23499652762006207 2113 330-339 0.14724919076599718 2113 340-349 0.1837313798113236 2113 350-351 0.013852661305921998 1112 1 -0.33122386788480185 1112 2 -0.013614663256888093 1112 3 0.06532589812855605 1112 4 0.1977764284054757 1112 5 0.16746958666185208 1112 6 0.05749231019803602 1112 7 0.03311791866052971 1112 8 0.14352336100403562 1112 9 0.22371777151547434 1112 10-19 0.059686510791337355 1112 20-29 0.13604607729885032 1112 30-39 0.1802904570041406 1112 40-49 0.23157243438619446 1112 50-59 0.2227701697690776 1112 60-69 0.22393303285601007 1112 70-79 0.20750206244137814 1112 80-89 0.25639041808127416 1112 90-99 0.25735676240619654 1112 100-109 0.1752888205009313 1112 110-119 0.2854626716183759 1112 120-129 0.31459944221776226 1112 130-139 0.20918517478438758 1112 140-149 0.6154391657029947 1112 150-159 0.405386588687918 1112 160-169 0.21738366202608006 1112 170-179 0.3189681667386495 1112 180-189 0.20831314523163513 1112 190-199 0.3113491216277495 1112 200-209 -0.1960553672978449 1112 210-219 -0.1446555170936854 1112 220-229 0.0807155285129646 1112 230-239 -0.19071077163228978 1112 240-249 -0.17123738652772857 1112 250-259 -0.1235168559310118 1112 260-269 0.14764024915248442 1112 270-279 0.1852879531354077 1112 280-289 0.3542357012746997 1112 290-299 0.214705792147722 1112 300-309 0.33998832400952494 1112 310-319 0.36604536311877567 1112 320-329 0.4291457134489782 1112 330-339 0.48427705077351746 1112 340-349 0.4057191207233046 1112 350-351 0.3560133138355148 2114 1 0.044329535299517886 2114 2 0.27791421003028205 2114 3 0.16083945271613942 2114 4 0.2312223152953905 2114 5 0.28905864310273444 2114 6 0.13065123011199375 2114 7 0.06201796182686792 2114 8 0.17620670200741984 2114 9 0.17779913114816992 2114 10-19 0.19105006769777333 2114 20-29 0.24570374324846256 2114 30-39 0.2150116238509625 2114 40-49 0.17161180137500764 2114 50-59 0.1485097946856868 2114 60-69 0.24474741402550393 2114 70-79 0.2631770091969372 2114 80-89 0.29117481826534686 2114 90-99 0.3700644335286185 2114 100-109 0.2977837457803574 2114 110-119 0.31531094728362064 2114 120-129 0.26572390754376585 2114 130-139 0.06440803439389953 2114 140-149 0.5841671143029288 2114 150-159 0.32192571844022666 2114 160-169 0.3818552667793682 2114 170-179 0.4294885351865432 2114 180-189 0.11405177125772781 2114 190-199 0.04910361574321698 2114 200-209 -0.43000872624558895 2114 210-219 0.23596315288359548 2114 220-229 0.2807450492952377 2114 230-239 0.04401842201334105 2114 240-249 -0.37211667774355917 2114 250-259 -0.0780263115000075 2114 260-269 0.08873852927706238 2114 270-279 0.1313802399168793 2114 280-289 0.17981782256141443 2114 290-299 0.2311749995637733 2114 300-309 0.34362820970791574 2114 310-319 0.3951572245869279 2114 320-329 0.312851401060243 2114 330-339 0.22961125100112056 2114 340-349 0.20533766940672749 2114 350-351 0.02272591162867954 1114 1 0.35669950557125674 1114 2 0.14874785739451823 1114 3 0.31531535607836503 1114 4 0.12058077110077647 1114 5 0.02061615262833527 1114 6 0.10805339901874333 1114 7 0.07843583227865025 1114 8 0.12190397621920823 1114 9 -0.021252411790321446 1114 10-19 -0.040889336899638806 1114 20-29 0.16476860109713698 1114 30-39 0.10760479891272468 1114 40-49 0.10346470092698468 1114 50-59 -0.039808038082391306 1114 60-69 0.04066216510366871 1114 70-79 0.143987342907117 1114 80-89 0.05758299144293488 1114 90-99 0.15269028455235656 1114 100-109 -0.05975828830246144 1114 110-119 0.004524680901681677 1114 120-129 0.05267273306820641 1114 130-139 -0.26942272388605915 1114 140-149 0.18319849227053453 1114 150-159 -0.11919615067169076 1114 160-169 0.07063451561744927 1114 170-179 0.2421728713252307 1114 180-189 0.041544330818922504 1114 190-199 0.17293574738497597 1114 200-209 0.043580286859963735 1114 210-219 0.2037223288665082 1114 220-229 0.09645727975204466 1114 230-239 -0.44409409316482495 1114 240-249 -0.1858987821692999 1114 250-259 -0.07744321892369399 1114 260-269 0.22050690008474305 1114 270-279 0.22247412259492805 1114 280-289 0.19527234669991955 1114 290-299 0.09713848823772508 1114 300-309 0.3572843668071073 1114 310-319 0.3193609008420033 1114 320-329 0.16403217399496128 1114 330-339 0.16318991364819269 1114 340-349 0.06748299453338902 1114 350-351 0.08201528063592534 1109 1 0.3897511642237532 1109 2 0.20976007510552108 1109 3 0.4046069533616574 1109 4 0.3593512914293271 1109 5 0.39179522722560023 1109 6 0.14531154260023982 1109 7 0.056872938196669054 1109 8 0.002874058730853335 1109 9 0.17307153529744568 1109 10-19 -0.025055348088294238 1109 20-29 0.11102781115570082 1109 30-39 0.17097832408718716 1109 40-49 0.27241833349757627 1109 50-59 0.09111798582915043 1109 60-69 0.009687779263494178 1109 70-79 0.1706064459437684 1109 80-89 0.07890060328269044 1109 90-99 0.12178810482290459 1109 100-109 -0.028474257393341418 1109 110-119 0.20610803637532626 1109 120-129 0.0614320797106771 1109 130-139 0.06859662238221631 1109 140-149 0.32984287642679533 1109 150-159 -0.4005772871246265 1109 160-169 -0.10096694377222803 1109 170-179 0.0346951831481519 1109 180-189 -0.16014038862276436 1109 190-199 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130-139 -0.3290519857198859 2105 140-149 -2.8217480375761 2105 150-159 -0.20659882086920334 2105 160-169 -0.3075948565551201 2105 170-179 0.09800206695581437 2105 180-189 -0.26550772699857816 2105 190-199 -0.061977724975172066 2105 200-209 0.20095528410149655 2105 210-219 0.006402854078906728 2105 220-229 -0.20961214538311523 2105 230-239 -0.27717329431234816 2105 240-249 0.1240943768288183 2105 250-259 0.12507956602610193 2105 260-269 -0.12082201969898776 2105 270-279 -0.49689332747679416 2105 280-289 -0.12649024846723833 2105 290-299 -0.14281693491134106 2105 300-309 -0.26760143259706126 2105 310-319 -0.24776812928046965 2105 320-329 -0.34801399173382563 2105 330-339 -0.23007183191067426 2105 340-349 -0.3155055081699061 2105 350-351 -0.13730370866121788 2104 1 0.14358088277918313 2104 2 -0.22744419855455789 2104 3 -0.16114550646982906 2104 4 -0.14577518693742064 2104 5 -0.33061293371614653 2104 6 -0.08934066535855578 2104 7 -0.0712663106425353 2104 8 -0.27342086911389174 2104 9 -0.029921110795072536 2104 10-19 -0.023870974127291333 2104 20-29 -0.09122001183211381 2104 30-39 -0.05857109994857268 2104 40-49 0.04204402487570036 2104 50-59 0.11198581110616601 2104 60-69 -0.15825624249966808 2104 70-79 -0.02136065314775948 2104 80-89 0.08952799278785761 2104 90-99 0.009539455179101708 2104 100-109 0.08314876809491523 2104 110-119 0.03264979522069922 2104 120-129 0.0026439695068347646 2104 130-139 -0.0068875315611123256 2104 140-149 -2.570509693867727 2104 150-159 0.42569290160124496 2104 160-169 -0.047820321646057096 2104 170-179 0.34771711950956075 2104 180-189 0.03098288546789263 2104 190-199 0.23544226581050154 2104 200-209 -0.1001830413925262 2104 210-219 0.0059236737961256836 2104 220-229 -0.21915001873266604 2104 230-239 -0.051720364123308826 2104 240-249 -0.08872286537561536 2104 250-259 -0.09296514127642652 2104 260-269 -0.19879140530393968 2104 270-279 -0.29287225954440643 2104 280-289 -0.28568581702284845 2104 290-299 -0.11174441322376794 2104 300-309 -0.32425647422221004 2104 310-319 -0.1959979622441601 2104 320-329 -0.22181480887959992 2104 330-339 -0.26815207505439886 2104 340-349 -0.2884691080399122 2104 350-351 0.08785200618062206 2111 1 -0.1868981480802958 2111 2 -0.1915283021733032 2111 3 -0.3189909996232174 2111 4 -0.14372417356651823 2111 5 -0.06875117991212676 2111 6 -0.052470054386887455 2111 7 -0.035652014684600886 2111 8 -0.02983913490982104 2111 9 -0.05838067480711118 2111 10-19 -0.038482870331073116 2111 20-29 -0.0312814704624671 2111 30-39 -0.2251310836892273 2111 40-49 -0.21877147396063634 2111 50-59 0.15891216188132518 2111 60-69 -0.31214349515959583 2111 70-79 -0.10626792529018303 2111 80-89 -0.005662532554737254 2111 90-99 0.1740937879489124 2111 100-109 0.002126595488782357 2111 110-119 -0.013619542928672956 2111 120-129 -0.16505796902214342 2111 130-139 0.04360192889872394 2111 140-149 -2.572734245740726 2111 150-159 0.2521532577017922 2111 160-169 -0.019368717666829127 2111 170-179 0.03725602156006147 2111 180-189 -0.026533848543692784 2111 190-199 0.35253754544580573 2111 200-209 -1.3950910671282202 2111 210-219 -0.4590750506412462 2111 220-229 0.12656694533692203 2111 230-239 -0.024651701663287895 2111 240-249 -0.04870424085623881 2111 250-259 0.0035975599672291025 2111 260-269 -0.046417488635039916 2111 270-279 -0.1679189431794228 2111 280-289 -0.13011617554402832 2111 290-299 -0.23619393128292643 2111 300-309 -0.4228926172078893 2111 310-319 -0.27593001814446794 2111 320-329 -0.3131070386479067 2111 330-339 -0.285347279199879 2111 340-349 -0.16428677304910622 2111 350-351 -0.06516535191058992 2110 1 0.3866690861751252 2110 2 0.1721391682897817 2110 3 0.15792615345808514 2110 4 0.22451225216394732 2110 5 0.1186233809996935 2110 6 -0.01646824844755912 2110 7 -0.04552742350028183 2110 8 0.027109457734148634 2110 9 -0.18295062728613942 2110 10-19 -0.041183245490863385 2110 20-29 -0.17898051418033134 2110 30-39 -0.11500679659967261 2110 40-49 -0.03140350176860096 2110 50-59 0.00743227514703193 2110 60-69 -0.3397100960212498 2110 70-79 -0.08820657321366099 2110 80-89 -0.16250508927388552 2110 90-99 -0.22895359768541823 2110 100-109 -0.26048377584104543 2110 110-119 -0.24685030592159762 2110 120-129 -0.2673183519404603 2110 130-139 -0.1465453050169998 2110 140-149 0.046541252604839656 2110 150-159 -0.15799479535206729 2110 160-169 -0.3446100894574542 2110 170-179 -3.37727069467131 2110 180-189 -0.31280350443445215 2110 190-199 -0.17610909863566349 2110 200-209 -2.0675043302622385 2110 210-219 -1.2433958700642975 2110 220-229 -0.13656556491326555 2110 230-239 -0.19322605611941768 2110 240-249 -0.15598529673898653 2110 250-259 -0.08037274230295921 2110 260-269 -0.04552487463101329 2110 270-279 -0.0753107881798627 2110 280-289 -0.22023535603330657 2110 290-299 -0.2524731373928759 2110 300-309 -0.41743515732511227 2110 310-319 -0.3208770179894067 2110 320-329 -0.2061814996741873 2110 330-339 -0.28223997867919337 2110 340-349 -0.3020846283865737 2110 350-351 -0.009075826961396416 2109 1 0.09109224552588557 2109 2 0.10342657413649903 2109 3 0.24858278899981912 2109 4 0.01137543856744827 2109 5 -0.1664254163814718 2109 6 -0.014247915364471453 2109 7 -0.05729266602445904 2109 8 -0.06172132143890252 2109 9 -0.3202671306323168 2109 10-19 -0.06317143616276155 2109 20-29 -0.15230293059993727 2109 30-39 -0.09339407233022712 2109 40-49 -0.08846548863669312 2109 50-59 -0.006317238764893318 2109 60-69 -0.003738712401819555 2109 70-79 -0.02829854387967856 2109 80-89 -0.08203575117421025 2109 90-99 0.03120484026833026 2109 100-109 -0.24840534734658348 2109 110-119 -0.07157232470460428 2109 120-129 -0.14120664367790425 2109 130-139 0.0743527997109723 2109 140-149 0.45071154422710435 2109 150-159 -0.01933577154431987 2109 160-169 -0.20506504750481724 2109 170-179 -3.288495570352538 2109 180-189 -0.2003607710879649 2109 190-199 0.24598708895585375 2109 200-209 0.03816550801759888 2109 210-219 -0.2877989131412555 2109 220-229 -0.16466313725056025 2109 230-239 -0.053818443942013516 2109 240-249 -0.5570440768273954 2109 250-259 -0.43208248541910876 2109 260-269 0.06778112054862717 2109 270-279 -0.23626015449430326 2109 280-289 -0.34126620166600574 2109 290-299 -0.10463819786656359 2109 300-309 -0.4050147725625095 2109 310-319 -0.828269391847325 2109 320-329 -0.5139516334038348 2109 330-339 -0.33265767039920746 2109 340-349 -0.1767960972109357 2109 350-351 -0.40347602868476073 2108 1 0.21009298928053255 2108 2 -0.011719978862792857 2108 3 -0.03943151108955334 2108 4 0.059651781961264305 2108 5 -0.14195026341176487 2108 6 -0.08005353498292322 2108 7 -0.13332022453003844 2108 8 -0.03289301626200114 2108 9 -0.05547797570009294 2108 10-19 -0.05694681392553491 2108 20-29 -0.2375572134391959 2108 30-39 -0.05269194294191948 2108 40-49 -0.010056769415534461 2108 50-59 0.15087968121585504 2108 60-69 0.05813250762665234 2108 70-79 0.01124816162056419 2108 80-89 0.04802777005613024 2108 90-99 0.03353506316556576 2108 100-109 0.08267078437923914 2108 110-119 0.2110205873344313 2108 120-129 0.25573104551599357 2108 130-139 0.3154310772835345 2108 140-149 0.72112865100069 2108 150-159 0.4678317524193716 2108 160-169 0.28735698846625013 2108 170-179 0.36472090808378255 2108 180-189 0.5251339715875147 2108 190-199 0.4899823639263232 2108 200-209 0.5139331690654814 2108 210-219 0.08343694874955077 2108 220-229 0.36066212519899565 2108 230-239 0.46690908143834875 2108 240-249 0.5057236071372984 2108 250-259 0.43739888633945867 2108 260-269 0.5756313779460669 2108 270-279 0.5416413740569155 2108 280-289 0.41703207936491893 2108 290-299 0.30093631614081673 2108 300-309 0.2004756784194761 2108 310-319 0.046046280958965013 2108 320-329 -0.07030667312283612 2108 330-339 0.08956184347275098 2108 340-349 -0.02169929452258401 2108 350-351 0.21708462065263134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 7.0 17 69.0 18 467.0 19 1559.0 20 3575.0 21 7333.0 22 10471.0 23 16185.0 24 26251.0 25 36250.0 26 46844.0 27 50985.0 28 56676.0 29 52106.0 30 49165.0 31 50413.0 32 50830.0 33 50478.0 34 59704.0 35 68311.0 36 55775.0 37 7915.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0406346997523422 0.02922846824291281 99.87025411658024 0.059882715424504296 2 99.7293871574388 0.053324132306582395 0.17194894000464803 0.04533977024998182 3 0.20445669980652179 0.09153500786317084 99.64269979796713 0.06130849436318296 4 0.033078071377345225 99.8571369503444 0.02110152829244437 0.0886834499858135 5 0.0029941357712252145 0.17879267891030567 0.054037021775921734 99.76417616354254 6 0.012832010448108062 0.013830055705183133 99.9334161235637 0.039921810283002866 7 0.1240427676650446 0.18820281990558493 99.4533563549106 0.23439805751877393 8 36.17158394059635 10.783166113226809 11.121646033269124 41.92360391290772 9 19.347677619975734 7.362579861442803 16.03259900965395 57.25714350892751 10-19 21.610331764501243 16.300745254651247 27.122592750484408 34.9663302303631 20-29 21.334500571024464 19.832071756602428 26.933990712475993 31.899436959897116 30-39 24.385681757586212 21.980463976982225 24.202184008178268 29.431670257253295 40-49 21.52723736795505 22.67460445327794 28.42959004578176 27.368568132985256 50-59 22.08250697562346 18.905629117827797 27.866735294159582 31.14512861238916 60-69 22.73936333267272 22.70618545676967 27.66832389705306 26.88612731350455 70-79 23.41803410748377 18.200567174861806 26.74337832616404 31.63802039149038 80-89 20.47706563288188 26.629743744751348 23.18047082072113 29.712719801645633 90-99 22.136173294875324 21.97205188124402 24.623230786559468 31.26854403732119 100-109 20.123133138258233 24.097706363352113 24.6874656476338 31.091694850755857 110-119 21.092118151449093 24.37163783504023 24.17155827657545 30.364685736935233 120-129 18.376855723106807 28.053220921087675 25.281947865522774 28.28797549028274 130-139 20.56676138591416 25.78934686492598 23.87950171877651 29.764390030383346 140-149 21.997316684037408 25.974498517902795 23.84405685436096 28.18412794369884 150-159 22.051510541496583 23.808269803000123 23.727542199492138 30.412677456011156 160-169 22.774993548350302 21.77211490067311 29.836591475838038 25.616300075138547 170-179 17.75554132627972 24.66687739682385 27.36513590740648 30.212445369489956 180-189 19.887808290877196 26.974187266680154 26.044294110291716 27.093710332150934 190-199 18.48697764806358 27.831532812163605 21.63154735510878 32.04994218466404 200-209 18.87575904906248 24.760875485299504 26.196435267497513 30.166930198140502 210-219 17.69351741089951 25.176247663504764 24.985364379194465 32.14487054640126 220-229 15.645457254434527 26.516351545758237 28.26886198602451 29.56932921378272 230-239 18.631294992236633 28.158107478067958 26.73938614513574 26.471211384559666 240-249 16.941490309693442 28.551066411357183 27.198116260866218 27.309327018083152 250-259 18.195052004581314 29.192827931697764 26.307792353517947 26.304327710202973 260-269 15.795197062666425 30.441158941889224 23.856111489101057 29.907532506343294 270-279 17.836366202765728 30.287109104881726 24.155247365516967 27.721277326835587 280-289 14.694136868239044 27.889722827109026 26.623474113340034 30.792666191311902 290-299 14.484932294738776 29.352215781977836 24.172987502047775 31.989864421235616 300-309 14.71952999475741 31.82356154211702 26.265589290915837 27.191319172209738 310-319 13.929061794684984 31.100715598449323 22.392585379207294 32.5776372276584 320-329 11.290130330452785 34.21159414917355 24.776473506888653 29.721802013485014 330-339 13.957039557392466 35.52634355465041 20.567964905296883 29.94865198266024 340-349 9.781415225524755 38.72712711633744 21.65725723721928 29.834200420918517 350-351 7.705194540407288 41.59517573438309 17.19304048784452 33.506589237365105 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.5 1 0.25 2 0.0 3 0.0 4 0.0 5 0.25 6 0.25 7 0.5 8 0.5 9 0.5 10 0.5 11 0.25 12 0.0 13 0.25 14 0.25 15 1.0 16 0.75 17 0.5 18 1.0 19 0.5 20 0.25 21 0.0 22 0.0 23 0.5 24 0.0 25 1.5 26 1.75 27 2.0 28 3.5 29 6.25 30 13.25 31 16.75 32 31.0 33 63.25 34 104.0 35 183.75 36 314.25 37 537.75 38 840.25 39 1356.75 40 2102.0 41 3207.75 42 4748.75 43 6865.5 44 9461.5 45 12444.75 46 14619.25 47 15291.0 48 15911.25 49 21122.25 50 26208.0 51 24628.75 52 23418.75 53 26086.0 54 17322.5 55 4715.75 56 1968.25 57 1916.25 58 1205.0 59 171.25 60 99.25 61 74.5 62 46.0 63 25.25 64 19.0 65 17.0 66 15.25 67 12.75 68 8.0 69 7.25 70 7.25 71 5.75 72 5.25 73 4.0 74 3.5 75 2.0 76 0.75 77 1.75 78 0.75 79 1.0 80 0.75 81 0.75 82 0.25 83 0.0 84 0.75 85 0.25 86 0.25 87 0.5 88 0.5 89 0.25 90 0.0 91 0.25 92 0.25 93 0.0 94 0.0 95 0.0 96 0.25 97 0.5 98 0.75 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-19 0.0 20-29 0.0 30-39 0.0 40-49 0.0 50-59 0.0 60-69 0.0 70-79 0.0 80-89 0.0 90-99 0.0 100-109 1.4257789386786737E-5 110-119 0.0 120-129 2.8515578773573475E-5 130-139 0.0 140-149 0.0 150-159 0.0 160-169 0.0 170-179 1.4257789386786737E-5 180-189 1.4257789386786737E-5 190-199 0.0 200-209 0.0 210-219 0.0 220-229 0.0 230-239 0.0 240-249 0.0 250-259 1.4257789386786737E-5 260-269 2.8515578773573475E-5 270-279 0.0 280-289 1.4257789386786737E-5 290-299 1.4257789386786737E-5 300-309 1.4257789386786737E-5 310-319 0.0 320-329 0.0 330-339 2.8515578773573475E-5 340-349 1.4257789386786737E-5 350-351 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 351 701371.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.600136255416176 #Duplication Level Percentage of deduplicated Percentage of total 1 71.43076636185904 4.714527908170681 2 10.058144456879672 1.3277024778412954 3 4.623490889741518 0.9154700954390816 4 2.5909488908103113 0.6840246284066985 5 1.6400512142665875 0.5412280740010114 6 1.1795924732652157 0.46712826296482723 7 0.9228040803173054 0.42634428670037594 8 0.6627949836470705 0.3499629761181601 9 0.5774620882562828 0.34301956183457655 >10 4.085548930708904 5.7547503006561405 >50 0.8141784484878265 3.8442989802615326 >100 0.9923237869452832 13.959093322654248 >500 0.1851164895616786 8.682125376998794 >1k 0.1851164895616786 23.443102381027046 >5k 0.03444027712775416 15.108477964103836 >10k+ 0.01722013856387708 19.438743402821654 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTACTTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 33556 4.784343806630157 No Hit TGTACTTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG 22055 3.1445554492558148 No Hit TGTACTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA 18910 2.6961479730413718 No Hit TGTACTTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT 14937 2.129686000704335 No Hit TGTACTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 13763 1.9622995533034586 No Hit TGTACTTCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC 11503 1.6400735131620783 No Hit TGTACTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT 11237 1.6021477933932258 No Hit TGTACTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA 10865 1.5491088168743787 No Hit TGTACTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC 9569 1.3643278664216227 No Hit TGTACTTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGT 9114 1.299454924711743 No Hit TGTACTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC 8408 1.198794931641029 No Hit TGTACTTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT 7886 1.1243692710420021 No Hit TGTACTTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC 7052 1.0054593075562006 No Hit TGTACTTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG 7039 1.0036057949359185 No Hit TGTACTTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACT 6739 0.9608324267755581 No Hit TGTACTTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA 6234 0.8888305903722851 No Hit TGTACTTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA 6111 0.8712935094265375 No Hit TGTACTTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT 6088 0.8680142178675765 No Hit TGTACTTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT 5650 0.8055651003534506 No Hit TGTACTTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG 5606 0.7992916730232644 No Hit TGTACTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACT 5374 0.7662136016459192 No Hit TGTACTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC 5329 0.7597975964218652 No Hit TGTACTTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 5145 0.7335632639501776 No Hit TGTACTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC 5002 0.7131746251270725 No Hit TGTACTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA 4396 0.6267724214431449 No Hit TGTACTTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC 4318 0.6156513457214513 No Hit TGTACTTGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTC 4255 0.6066689384077757 No Hit TGTACTTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA 4037 0.5755869575445806 No Hit TGTACTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC 3936 0.5611865902639259 No Hit TGTACTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA 3712 0.5292491420375236 No Hit TGTACTTGAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAG 3544 0.505296055867722 No Hit TGTACTTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC 3501 0.4991652064314036 No Hit TGTACTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT 3408 0.485905462301692 No Hit TGTACTTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC 3093 0.44099342573331374 No Hit TGTACTTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC 3022 0.43087039526869514 No Hit TGTACTTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT 2943 0.41960674165313366 No Hit TGTACTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT 2926 0.4171829174573799 No Hit TGTACTTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT 2856 0.4072024648866292 No Hit TGTACTTCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCC 2703 0.3853880471248455 No Hit TGTACTTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC 2528 0.3604369156979687 No Hit TGTACTTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC 2507 0.35744277992674345 No Hit TGTACTTCCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 2504 0.35701504624513986 No Hit TGTACTTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC 2467 0.3517396641720288 No Hit TGTACTTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA 2318 0.33049555798571656 No Hit TGTACTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCA 2287 0.3260756432758127 No Hit TGTACTTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 2183 0.31124754231355445 No Hit TGTACTTGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGC 2148 0.30625731602817907 No Hit TGTACTTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC 2095 0.2987006876531821 No Hit TGTACTTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG 2088 0.29770264239610705 No Hit TGTACTTGCCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAAGACCTT 2061 0.2938530392616746 No Hit TGTACTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA 1984 0.2828745414338489 No Hit TGTACTTGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC 1967 0.2804507172380951 No Hit TGTACTTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT 1908 0.27203862149989094 No Hit TGTACTTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCG 1889 0.2693296415164015 No Hit TGTACTTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT 1880 0.2680464404715906 No Hit TGTACTTACCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 1866 0.26605034995744054 No Hit TGTACTTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCAC 1806 0.25749567632536846 No Hit TGTACTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTA 1776 0.2532183395093324 No Hit TGTACTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT 1756 0.25036678163197507 No Hit TGTACTTCCGTCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAAT 1722 0.24551913324046762 No Hit TGTACTTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGT 1713 0.2442359321956568 No Hit TGTACTTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGC 1696 0.24181210799990305 No Hit TGTACTTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC 1693 0.24138437431829946 No Hit TGTACTTGTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTAC 1683 0.23995859537962078 No Hit TGTACTTGGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 1672 0.23839023854707425 No Hit TGTACTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTT 1666 0.23753477118386704 No Hit TGTACTTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG 1656 0.23610899224518833 No Hit TGTACTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTCCGAGCGT 1653 0.23568125856358474 No Hit TGTACTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCAC 1639 0.23368516804943462 No Hit TGTACTTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC 1635 0.23311485647396313 No Hit TGTACTTGCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTG 1635 0.23311485647396313 No Hit TGTACTTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT 1598 0.22783947440085206 No Hit TGTACTTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA 1593 0.22712658493151272 No Hit TGTACTTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG 1582 0.22555822809896617 No Hit TGTACTTCACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGC 1576 0.22470276073575896 No Hit TGTACTTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT 1573 0.22427502705415536 No Hit TGTACTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCG 1560 0.2224215144338731 No Hit TGTACTTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT 1551 0.22113831338906229 No Hit TGTACTTGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC 1544 0.2201402681319872 No Hit TGTACTTTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTC 1537 0.21914222287491214 No Hit TGTACTTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCT 1504 0.2144371523772725 No Hit TGTACTTCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCT 1502 0.21415199658953676 No Hit TGTACTTGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT 1407 0.2006070966720894 No Hit TGTACTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG 1352 0.19276531250935666 No Hit TGTACTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA 1340 0.19105437778294226 No Hit TGTACTTATCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTC 1330 0.18962859884426359 No Hit TGTACTTCCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCC 1301 0.18549383992209545 No Hit TGTACTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT 1300 0.18535126202822758 No Hit TGTACTTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT 1284 0.18307001572634168 No Hit TGTACTTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG 1274 0.181644236787663 No Hit TGTACTTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA 1265 0.1803610357428522 No Hit TGTACTTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 1240 0.17679658839615553 No Hit TGTACTTCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGT 1226 0.17480049788200538 No Hit TGTACTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGG 1220 0.1739450305187982 No Hit TGTACTTCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC 1192 0.1699528494904979 No Hit TGTACTTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGG 1177 0.16781418108247986 No Hit TGTACTTCCCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGA 1163 0.16581809056832975 No Hit TGTACTTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT 1162 0.16567551267446187 No Hit TGTACTTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCC 1137 0.16211106532776517 No Hit TGTACTTCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCAT 1132 0.16139817585842586 No Hit TGTACTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTT 1131 0.161255597964558 No Hit TGTACTTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT 1115 0.1589743516626721 No Hit TGTACTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCG 1093 0.15583763799757902 No Hit TGTACTTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG 1067 0.15213061275701448 No Hit TGTACTTAGGGCTGGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGG 1064 0.15170287907541088 No Hit TGTACTTGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA 1060 0.15113256749993942 No Hit TGTACTTTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCG 1043 0.14870874330418565 No Hit TGTACTTGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACT 1035 0.14756812015324272 No Hit TGTACTTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGC 1026 0.14628491910843192 No Hit TGTACTTGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC 1025 0.14614234121456404 No Hit TGTACTTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA 993 0.14157984861079229 No Hit TGTACTTTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTT 992 0.14143727071692444 No Hit TGTACTTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA 981 0.1398689138843779 No Hit TGTACTTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA 981 0.1398689138843779 No Hit TGTACTTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC 975 0.13901344652117067 No Hit TGTACTTGCCACTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGT 968 0.1380154012640956 No Hit TGTACTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCCTTTTCACGACG 959 0.13673220021928478 No Hit TGTACTTGCCGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGA 956 0.1363044665376812 No Hit TGTACTTGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATG 922 0.13145681814617372 No Hit TGTACTTGGCCGCGTCTGGCGCGACATGGAACGACATCGATGCCGGGCTT 920 0.13117166235843797 No Hit TGTACTTGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC 903 0.12874783816268423 No Hit TGTACTTGGCTCAGCAATCGTTGGCTACATCTTTGATTTCTCGCGGAGTA 884 0.12603885817919475 No Hit TGTACTTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCA 876 0.12489823502825181 No Hit TGTACTTGCGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCC 868 0.12375761187730888 No Hit TGTACTTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT 843 0.1201931645306122 No Hit TGTACTTCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGCG 837 0.119337697167405 No Hit TGTACTTCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTC 837 0.119337697167405 No Hit TGTACTTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCT 835 0.11905254137966924 No Hit TGTACTTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC 826 0.11776934033485845 No Hit TGTACTTAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT 816 0.11634356139617977 No Hit TGTACTTGTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCC 808 0.11520293824523684 No Hit TGTACTTGCCTTCGCCACTGGTGTTCCTTCCTATATCTACGCATTTCACC 798 0.11377715930655816 No Hit TGTACTTCGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCG 788 0.11235138036787948 No Hit TGTACTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC 784 0.11178106879240803 No Hit TGTACTTGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTAC 784 0.11178106879240803 No Hit TGTACTTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA 779 0.11106817932306867 No Hit TGTACTTGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAA 770 0.10978497827825787 No Hit TGTACTTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGG 768 0.10949982249052215 No Hit TGTACTTCCTTCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCA 768 0.10949982249052215 No Hit TGTACTTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA 762 0.10864435512731493 No Hit TGTACTTCCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT 760 0.10835919933957919 No Hit TGTACTTTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTC 760 0.10835919933957919 No Hit TGTACTTGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCT 760 0.10835919933957919 No Hit TGTACTTGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT 749 0.10679084250703266 No Hit TGTACTTCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGA 747 0.10650568671929692 No Hit TGTACTTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGCCT 742 0.10579279724995758 No Hit TGTACTTCCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG 731 0.10422444041741105 No Hit TGTACTTTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGAC 727 0.10365412884193957 No Hit TGTACTTAGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACAT 724 0.10322639516033598 No Hit TGTACTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACC 721 0.10279866147873237 No Hit TGTACTTCGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT 720 0.1026560835848645 No Hit TGTACTTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG 716 0.10208577200939302 No Hit TGTACTTACCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG 712 0.10151546043392157 No Hit TGTACTTCCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT 704 0.10037483728297862 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 9.980452570750714E-4 0.0 0.0 8 0.0017109347264144083 0.0 0.0 9 0.001996090514150143 0.0 0.0 10-19 0.4133475721123343 0.0 0.0 20-29 0.6617467788089326 0.0 0.0 30-39 0.6750350385174181 0.0 0.0 40-49 0.7920059426466164 0.0 0.0 50-59 1.4155418458989608 0.0 0.0 60-69 2.5091570652336634 0.0 0.0 70-79 4.07691507062596 0.0 0.0 80-89 5.928174960185123 0.0 0.0 90-99 8.982749500620926 0.0 4.27733681603602E-5 100-109 12.118194222458582 0.0 1.4257789386786735E-4 110-119 15.749310992327883 0.0 1.4257789386786735E-4 120-129 18.96187609695867 0.0 1.4257789386786735E-4 130-139 24.037406736235177 0.0 1.4257789386786735E-4 140-149 27.477754284109267 0.0 1.4257789386786735E-4 150-159 30.491408969004986 0.0 1.4257789386786735E-4 160-169 33.50455037348279 0.0 1.4257789386786735E-4 170-179 36.67251711291171 2.851557877357347E-5 1.4257789386786735E-4 180-189 39.66802163191806 1.4257789386786735E-4 1.4257789386786735E-4 190-199 42.53898436063082 1.4257789386786735E-4 1.568356832546541E-4 200-209 45.41053165870845 1.4257789386786735E-4 3.992181028300286E-4 210-219 47.156483515856806 1.4257789386786735E-4 4.2773368160360203E-4 220-229 48.85296084383301 1.4257789386786735E-4 4.2773368160360203E-4 230-239 51.27419012191835 1.4257789386786735E-4 4.2773368160360203E-4 240-249 53.1936735336933 1.4257789386786735E-4 4.419914709903888E-4 250-259 54.5094678850423 1.4257789386786735E-4 8.412095738204175E-4 260-269 55.845251657111575 1.4257789386786735E-4 9.980452570750712E-4 270-279 57.10735117362994 1.4257789386786735E-4 0.0010693342040090053 280-289 58.26824890108088 1.4257789386786735E-4 0.0011406231509429388 290-299 59.41870992670071 1.4257789386786735E-4 0.001226169887263659 300-309 60.44095065236515 1.4257789386786735E-4 0.0014828100962258203 310-319 61.286195180582034 1.4257789386786735E-4 0.0015683568325465407 320-329 62.047689453940926 1.4257789386786735E-4 0.0015683568325465407 330-339 62.66871598626119 1.4257789386786735E-4 0.0015683568325465407 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCGGG 25 2.0736479E-10 345.00003 6 TGTACTT 69320 0.0 343.8304 1 GTACTTG 25030 0.0 342.93246 2 GTACTTC 29435 0.0 341.8354 2 TACTTGC 16790 0.0 339.14383 3 TACTTGG 3080 0.0 337.1591 3 GTACTTT 8040 0.0 335.9888 2 ACTTGCC 13250 0.0 335.7566 4 CTTGCCC 7690 0.0 332.2139 5 TGCCGCG 2455 0.0 331.64972 7 TACTTCC 16730 0.0 331.49286 3 TTGCCCC 5550 0.0 330.0811 6 CTTGCCG 2875 0.0 330.0 5 TACTTCG 5630 0.0 326.92273 3 TACTTGT 4150 0.0 323.38556 3 ACTTCCC 12790 0.0 321.53244 4 TTGCCGC 2700 0.0 319.44446 6 ACTTCGC 3765 0.0 318.4263 4 ACTTGCG 2140 0.0 318.39954 4 TGGCCCG 435 0.0 317.2414 7 >>END_MODULE