FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 ss.3410000000ebd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 ss.3410000000ebd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701371
Sequences flagged as poor quality0
Sequence length351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTACTTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT335564.784343806630157No Hit
TGTACTTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG220553.1445554492558148No Hit
TGTACTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA189102.6961479730413718No Hit
TGTACTTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT149372.129686000704335No Hit
TGTACTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA137631.9622995533034586No Hit
TGTACTTCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC115031.6400735131620783No Hit
TGTACTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT112371.6021477933932258No Hit
TGTACTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA108651.5491088168743787No Hit
TGTACTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC95691.3643278664216227No Hit
TGTACTTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGT91141.299454924711743No Hit
TGTACTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC84081.198794931641029No Hit
TGTACTTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT78861.1243692710420021No Hit
TGTACTTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC70521.0054593075562006No Hit
TGTACTTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG70391.0036057949359185No Hit
TGTACTTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACT67390.9608324267755581No Hit
TGTACTTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA62340.8888305903722851No Hit
TGTACTTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA61110.8712935094265375No Hit
TGTACTTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT60880.8680142178675765No Hit
TGTACTTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT56500.8055651003534506No Hit
TGTACTTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG56060.7992916730232644No Hit
TGTACTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACT53740.7662136016459192No Hit
TGTACTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC53290.7597975964218652No Hit
TGTACTTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA51450.7335632639501776No Hit
TGTACTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC50020.7131746251270725No Hit
TGTACTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA43960.6267724214431449No Hit
TGTACTTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC43180.6156513457214513No Hit
TGTACTTGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTC42550.6066689384077757No Hit
TGTACTTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA40370.5755869575445806No Hit
TGTACTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC39360.5611865902639259No Hit
TGTACTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA37120.5292491420375236No Hit
TGTACTTGAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAG35440.505296055867722No Hit
TGTACTTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC35010.4991652064314036No Hit
TGTACTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT34080.485905462301692No Hit
TGTACTTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC30930.44099342573331374No Hit
TGTACTTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC30220.43087039526869514No Hit
TGTACTTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT29430.41960674165313366No Hit
TGTACTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT29260.4171829174573799No Hit
TGTACTTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT28560.4072024648866292No Hit
TGTACTTCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCC27030.3853880471248455No Hit
TGTACTTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC25280.3604369156979687No Hit
TGTACTTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC25070.35744277992674345No Hit
TGTACTTCCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT25040.35701504624513986No Hit
TGTACTTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC24670.3517396641720288No Hit
TGTACTTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA23180.33049555798571656No Hit
TGTACTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCA22870.3260756432758127No Hit
TGTACTTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA21830.31124754231355445No Hit
TGTACTTGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGC21480.30625731602817907No Hit
TGTACTTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC20950.2987006876531821No Hit
TGTACTTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG20880.29770264239610705No Hit
TGTACTTGCCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAAGACCTT20610.2938530392616746No Hit
TGTACTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA19840.2828745414338489No Hit
TGTACTTGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC19670.2804507172380951No Hit
TGTACTTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT19080.27203862149989094No Hit
TGTACTTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCG18890.2693296415164015No Hit
TGTACTTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT18800.2680464404715906No Hit
TGTACTTACCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT18660.26605034995744054No Hit
TGTACTTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCAC18060.25749567632536846No Hit
TGTACTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTA17760.2532183395093324No Hit
TGTACTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT17560.25036678163197507No Hit
TGTACTTCCGTCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAAT17220.24551913324046762No Hit
TGTACTTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGT17130.2442359321956568No Hit
TGTACTTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGC16960.24181210799990305No Hit
TGTACTTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC16930.24138437431829946No Hit
TGTACTTGTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTAC16830.23995859537962078No Hit
TGTACTTGGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA16720.23839023854707425No Hit
TGTACTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTT16660.23753477118386704No Hit
TGTACTTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG16560.23610899224518833No Hit
TGTACTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTCCGAGCGT16530.23568125856358474No Hit
TGTACTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCAC16390.23368516804943462No Hit
TGTACTTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC16350.23311485647396313No Hit
TGTACTTGCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTG16350.23311485647396313No Hit
TGTACTTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT15980.22783947440085206No Hit
TGTACTTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA15930.22712658493151272No Hit
TGTACTTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG15820.22555822809896617No Hit
TGTACTTCACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGC15760.22470276073575896No Hit
TGTACTTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT15730.22427502705415536No Hit
TGTACTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCG15600.2224215144338731No Hit
TGTACTTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT15510.22113831338906229No Hit
TGTACTTGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC15440.2201402681319872No Hit
TGTACTTTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTC15370.21914222287491214No Hit
TGTACTTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCT15040.2144371523772725No Hit
TGTACTTCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCT15020.21415199658953676No Hit
TGTACTTGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT14070.2006070966720894No Hit
TGTACTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG13520.19276531250935666No Hit
TGTACTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA13400.19105437778294226No Hit
TGTACTTATCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTC13300.18962859884426359No Hit
TGTACTTCCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCC13010.18549383992209545No Hit
TGTACTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT13000.18535126202822758No Hit
TGTACTTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT12840.18307001572634168No Hit
TGTACTTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG12740.181644236787663No Hit
TGTACTTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA12650.1803610357428522No Hit
TGTACTTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT12400.17679658839615553No Hit
TGTACTTCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGT12260.17480049788200538No Hit
TGTACTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGG12200.1739450305187982No Hit
TGTACTTCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC11920.1699528494904979No Hit
TGTACTTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGG11770.16781418108247986No Hit
TGTACTTCCCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGA11630.16581809056832975No Hit
TGTACTTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT11620.16567551267446187No Hit
TGTACTTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCC11370.16211106532776517No Hit
TGTACTTCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCAT11320.16139817585842586No Hit
TGTACTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTT11310.161255597964558No Hit
TGTACTTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT11150.1589743516626721No Hit
TGTACTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCG10930.15583763799757902No Hit
TGTACTTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG10670.15213061275701448No Hit
TGTACTTAGGGCTGGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGG10640.15170287907541088No Hit
TGTACTTGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA10600.15113256749993942No Hit
TGTACTTTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCG10430.14870874330418565No Hit
TGTACTTGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACT10350.14756812015324272No Hit
TGTACTTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGC10260.14628491910843192No Hit
TGTACTTGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC10250.14614234121456404No Hit
TGTACTTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA9930.14157984861079229No Hit
TGTACTTTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTT9920.14143727071692444No Hit
TGTACTTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA9810.1398689138843779No Hit
TGTACTTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA9810.1398689138843779No Hit
TGTACTTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC9750.13901344652117067No Hit
TGTACTTGCCACTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGT9680.1380154012640956No Hit
TGTACTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCCTTTTCACGACG9590.13673220021928478No Hit
TGTACTTGCCGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGA9560.1363044665376812No Hit
TGTACTTGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATG9220.13145681814617372No Hit
TGTACTTGGCCGCGTCTGGCGCGACATGGAACGACATCGATGCCGGGCTT9200.13117166235843797No Hit
TGTACTTGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC9030.12874783816268423No Hit
TGTACTTGGCTCAGCAATCGTTGGCTACATCTTTGATTTCTCGCGGAGTA8840.12603885817919475No Hit
TGTACTTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCA8760.12489823502825181No Hit
TGTACTTGCGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCC8680.12375761187730888No Hit
TGTACTTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT8430.1201931645306122No Hit
TGTACTTCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGCG8370.119337697167405No Hit
TGTACTTCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTC8370.119337697167405No Hit
TGTACTTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCT8350.11905254137966924No Hit
TGTACTTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC8260.11776934033485845No Hit
TGTACTTAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT8160.11634356139617977No Hit
TGTACTTGTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCC8080.11520293824523684No Hit
TGTACTTGCCTTCGCCACTGGTGTTCCTTCCTATATCTACGCATTTCACC7980.11377715930655816No Hit
TGTACTTCGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCG7880.11235138036787948No Hit
TGTACTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC7840.11178106879240803No Hit
TGTACTTGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTAC7840.11178106879240803No Hit
TGTACTTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA7790.11106817932306867No Hit
TGTACTTGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAA7700.10978497827825787No Hit
TGTACTTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGG7680.10949982249052215No Hit
TGTACTTCCTTCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCA7680.10949982249052215No Hit
TGTACTTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA7620.10864435512731493No Hit
TGTACTTCCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT7600.10835919933957919No Hit
TGTACTTTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTC7600.10835919933957919No Hit
TGTACTTGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCT7600.10835919933957919No Hit
TGTACTTGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT7490.10679084250703266No Hit
TGTACTTCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGA7470.10650568671929692No Hit
TGTACTTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGCCT7420.10579279724995758No Hit
TGTACTTCCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG7310.10422444041741105No Hit
TGTACTTTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGAC7270.10365412884193957No Hit
TGTACTTAGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACAT7240.10322639516033598No Hit
TGTACTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACC7210.10279866147873237No Hit
TGTACTTCGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT7200.1026560835848645No Hit
TGTACTTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG7160.10208577200939302No Hit
TGTACTTACCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG7120.10151546043392157No Hit
TGTACTTCCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT7040.10037483728297862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGGG252.0736479E-10345.000036
TGTACTT693200.0343.83041
GTACTTG250300.0342.932462
GTACTTC294350.0341.83542
TACTTGC167900.0339.143833
TACTTGG30800.0337.15913
GTACTTT80400.0335.98882
ACTTGCC132500.0335.75664
CTTGCCC76900.0332.21395
TGCCGCG24550.0331.649727
TACTTCC167300.0331.492863
TTGCCCC55500.0330.08116
CTTGCCG28750.0330.05
TACTTCG56300.0326.922733
TACTTGT41500.0323.385563
ACTTCCC127900.0321.532444
TTGCCGC27000.0319.444466
ACTTCGC37650.0318.42634
ACTTGCG21400.0318.399544
TGGCCCG4350.0317.24147
Produced by FastQC (version 0.11.2)