FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 pt309.3410000000ea74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 pt309.3410000000ea74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580343
Sequences flagged as poor quality0
Sequence length351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCATGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT953516.033563599800803No Hit
TGACCATCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA696714.408599905210451No Hit
TGACCATCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT622203.9371199796499874No Hit
TGACCATGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA606543.8380275674331457No Hit
TGACCATCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA528203.342312396739189No Hit
TGACCATCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT501083.17070408132918No Hit
TGACCATGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG487213.0829383241486186No Hit
TGACCATCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTT467812.9601801634202194No Hit
TGACCATGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA254371.6095872857980829No Hit
TGACCATGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG249301.5775056427623624No Hit
TGACCATGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT235151.487968118313556No Hit
TGACCATGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT213001.3478086719148943No Hit
TGACCATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC210911.3345836948054948No Hit
TGACCATCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC210101.3294582252080718No Hit
TGACCATCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC208131.31699257692792No Hit
TGACCATCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT188561.1931587003580868No Hit
TGACCATCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC186381.1793642266267512No Hit
TGACCATCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT172101.08900409594626No Hit
TGACCATGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC146900.9295450418042158No Hit
TGACCATCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG137410.8694947868912003No Hit
TGACCATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT124240.7861584478812512No Hit
TGACCATACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA114610.7252223093341129No Hit
TGACCATGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT113640.7190844012976929No Hit
TGACCATGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC103840.6570725469091204No Hit
TGACCATCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC102890.651061193677575No Hit
TGACCATCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA101990.6453662274582164No Hit
TGACCATGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG97950.6198021568735395No Hit
TGACCATCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA96740.6121455911786239No Hit
TGACCATCTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAG96490.6105636561176909No Hit
TGACCATCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA92420.5848097533257021No Hit
TGACCATCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC90700.5739260401064832No Hit
TGACCATTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT85930.5437427191438821No Hit
TGACCATCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA82030.5190645321933277No Hit
TGACCATGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC79800.5049536714498055No Hit
TGACCATCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA75570.4781873302188196No Hit
TGACCATCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG72780.4605329349388076No Hit
TGACCATCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG67490.4270591890494659No Hit
TGACCATCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC67020.42408515113491185No Hit
TGACCATACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACA60980.38586560006277115No Hit
TGACCATCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG60890.3852961034408353No Hit
TGACCATCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT59840.3786519761849168No Hit
TGACCATCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC53460.3382809934299073No Hit
TGACCATCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGA53210.33669905836897435No Hit
TGACCATGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG52660.33321880123492176No Hit
TGACCATCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA52330.33113064695449024No Hit
TGACCATCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG52130.3298650989057439No Hit
TGACCATCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT51960.3287893830643095No Hit
TGACCATCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTA50280.31815877945483984No Hit
TGACCATCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCC49850.31543785115003514No Hit
TGACCATCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACT46700.2955054693822797No Hit
TGACCATGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA46360.2933540376994108No Hit
TGACCATCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT45620.2886715099190492No Hit
TGACCATGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTT45410.28734268446786554No Hit
TGACCATTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAAC44800.28348276291918906No Hit
TGACCATGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG43510.2753199780047749No Hit
TGACCATCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT39040.24703497911529332No Hit
TGACCATCCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACC38790.24545304405436036No Hit
TGACCATGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGA38540.24387110899342737No Hit
TGACCATAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC37400.23665748511557302No Hit
TGACCATGCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTG37020.2342529438229549No Hit
TGACCATGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC36730.23241789915227265No Hit
TGACCATCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTC36690.23216478954252334No Hit
TGACCATCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTCA33410.21140980154308273No Hit
TGACCATCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAACC32320.20451256467741494No Hit
TGACCATGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA31640.20020970131167729No Hit
TGACCATCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCAT31400.1986910436531816No Hit
TGACCATCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC31240.1976786052141845No Hit
TGACCATACCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTT30760.1946412898971932No Hit
TGACCATGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT30720.19438818028744392No Hit
TGACCATCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACC30710.19432490288500662No Hit
TGACCATGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT30400.19236330340944974No Hit
TGACCATAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT29330.1855926213486566No Hit
TGACCATCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTCG29120.18426379589747288No Hit
TGACCATCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGT28760.18198580940972942No Hit
TGACCATCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCT28530.18053042915367107No Hit
TGACCATTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT28530.18053042915367107No Hit
TGACCATCCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTG27940.17679706240986925No Hit
TGACCATCCGCCGTGTGTCTCCCATGCTCGGCACTTGTAGGTATTCGGAG27900.17654395280011997No Hit
TGACCATCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA27810.1759744561781841No Hit
TGACCATGCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT27750.1755947917635602No Hit
TGACCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC27350.17306369566606744No Hit
TGACCATATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC26710.169013941910079No Hit
TGACCATGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGC26690.16888738710520437No Hit
TGACCATCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGCG26030.16471107854434133No Hit
TGACCATCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC25980.16439469153215475No Hit
TGACCATGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA25690.16255964686147248No Hit
TGACCATCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTGCC25690.16255964686147248No Hit
TGACCATCCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCA25160.15920594453229456No Hit
TGACCATCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC24730.15648501622748986No Hit
TGACCATCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCA24070.1523087076666268No Hit
TGACCATCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACA23500.14870189572769962No Hit
TGACCATCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGA23420.14819567650820106No Hit
TGACCATTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA22890.14484197417902317No Hit
TGACCATGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC22530.14256398769127968No Hit
TGACCATACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCAC22470.14218432327665576No Hit
TGACCATGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA22300.14110860743522133No Hit
TGACCATCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCT22190.14041255600841085No Hit
TGACCATGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT22150.14015944639866157No Hit
TGACCATTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG21950.1388938983499152No Hit
TGACCATCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC21060.13326220953299378No Hit
TGACCATCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA20890.13218649369155935No Hit
TGACCATCACCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTT20680.13085766824037567No Hit
TGACCATGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC20470.12952884278919197No Hit
TGACCATCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTT20400.12908590097213074No Hit
TGACCATCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC19530.12358076696008398No Hit
TGACCATCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTA19360.12250505111864955No Hit
TGACCATCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCC19290.12206210930158833No Hit
TGACCATCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAA19220.1216191674845271No Hit
TGACCATCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCCC19010.1202903420333434No Hit
TGACCATCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGT18580.11756941372853867No Hit
TGACCATTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC18130.11472193061885932No Hit
TGACCATCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTC18010.11396260178961151No Hit
TGACCATCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATGAG17930.11345638257011295No Hit
TGACCATGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC17700.11200100231405462No Hit
TGACCATCGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTG17670.11181117010674266No Hit
TGACCATCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACAG17580.11124167348480678No Hit
TGACCATCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAG17260.10921679660681256No Hit
TGACCATCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTC17240.10909024180193792No Hit
TGACCATCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTGC17220.1089636869970633No Hit
TGACCATCCCGCTTAGATGCTTTCAGCGGTTATCTTTTCCGAACATAGCT17190.10877385478975134No Hit
TGACCATCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT16930.10712864232638103No Hit
TGACCATCCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT16820.10643259089957054No Hit
TGACCATCGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGG16670.10548342986301075No Hit
TGACCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCATCG16340.10339527558257923No Hit
TGACCATGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCCC16230.10269922415576871No Hit
TGACCATCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAA16210.10257266935089408No Hit
TGACCATGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA16030.10143367610702234No Hit
TGACCATGCCAGGGTGGTATTTCAAGGTTGGCTCCACGCAGACTGGCGTC15950.10092745688752378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCAT1568100.0343.217931
GACCATG535850.0341.587652
GACCATC901550.0340.981932
CCATGCC303750.0336.595064
ATGCCCC143150.0336.44436
ACCATGC357300.0335.730473
CATGCCC221650.0335.1945
ATGCCCG68850.0330.217866
ACCATCG206050.0329.09373
TGCCCGG68450.0328.115427
ACCATCC490950.0325.569823
TCCCGTT70500.0324.202127
TGCCGCG25950.0323.728337
ATCCGGG6300.0323.095256
CCATGCG37850.0322.668434
CCATCCC367800.0322.393954
ATGGCCT25750.0320.883486
CCATGGC44150.0320.77584
CCATGTC103850.0320.084264
ACCATGT120700.0319.989653
Produced by FastQC (version 0.11.2)