FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 pt305.3410000000ea5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 pt305.3410000000ea5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187416
Sequences flagged as poor quality0
Sequence length351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGTTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT794343.631408017496443No Hit
CGATGTTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG664083.03591086469149No Hit
CGATGTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA647512.9601593844060754No Hit
CGATGTTCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTT636602.910283183445673No Hit
CGATGTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT514192.3506731229907802No Hit
CGATGTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT493192.254669436449217No Hit
CGATGTTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT489952.23985743909709No Hit
CGATGTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA467602.1376820869921405No Hit
CGATGTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA452152.0670508033222763No Hit
CGATGTTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC388501.776068201018919No Hit
CGATGTTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT349511.5978213563400834No Hit
CGATGTTCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC258261.180662480296386No Hit
CGATGTTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT249051.138558006341729No Hit
CGATGTTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA237981.0879503487219622No Hit
CGATGTTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA234281.071035413474163No Hit
CGATGTTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT216140.9881065147187366No Hit
CGATGTTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT185530.8481697125741057No Hit
CGATGTTGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA181770.8309804810790449No Hit
CGATGTTCTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAG176620.8074367198557567No Hit
CGATGTTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC170210.7781327374399748No Hit
CGATGTTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG167680.766566579013777No Hit
CGATGTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC166250.7600291851207086No Hit
CGATGTTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC166210.7598463209558676No Hit
CGATGTTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT165040.7544975441342662No Hit
CGATGTTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA153840.7032955779787659No Hit
CGATGTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC144350.6599110548702213No Hit
CGATGTTCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT143990.6582652773866516No Hit
CGATGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC141120.6451447735593047No Hit
CGATGTTCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACT135070.6174865686270925No Hit
CGATGTTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG127860.584525302914489No Hit
CGATGTTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA124240.567976095996372No Hit
CGATGTTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA123300.5636787881226067No Hit
CGATGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA108170.494510417771471No Hit
CGATGTTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC107690.4923160477933781No Hit
CGATGTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA107040.4893445051147107No Hit
CGATGTTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT98640.4509430304980854No Hit
CGATGTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC93190.42602778803848923No Hit
CGATGTTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG89120.40742135926591005No Hit
CGATGTTGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC84230.38506621511408895No Hit
CGATGTTCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTC83510.38177466014694966No Hit
CGATGTTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG79930.36540831739367363No Hit
CGATGTTGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT77460.3541164552147374No Hit
CGATGTTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG77240.3531107023081115No Hit
CGATGTTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC76700.350642036082757No Hit
CGATGTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA76320.3489048265167668No Hit
CGATGTTCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAAG73830.33752153225541004No Hit
CGATGTTGCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTG72520.3315327308568649No Hit
CGATGTTCGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGG70890.3240810161395912No Hit
CGATGTTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA70560.32257238677965233No Hit
CGATGTTGTCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAG69770.31896081952404115No Hit
CGATGTTCCCGCTTAGATGCTTTCAGCGGTTATCTTTTCCGAACATAGCT69620.31827507890588713No Hit
CGATGTTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC68950.31521210414479917No Hit
CGATGTTGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTT67680.3094061669110951No Hit
CGATGTTCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCAT67270.3075318092214741No Hit
CGATGTTCCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACC65660.3001715265866209No Hit
CGATGTTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC65210.29811430473215883No Hit
CGATGTTAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT62540.28590812172901725No Hit
CGATGTTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA60560.27685634556938415No Hit
CGATGTTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC59450.27178186499504436No Hit
CGATGTTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT59430.27169043291262385No Hit
CGATGTTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA55570.25404404100546035No Hit
CGATGTTGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCT55270.25267255976915226No Hit
CGATGTTCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAG52230.23877488324123072No Hit
CGATGTTCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCC51650.23612335285103522No Hit
CGATGTTCGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTG50870.23255750163663427No Hit
CGATGTTACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCAC50130.22917451458707444No Hit
CGATGTTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCC50100.2290373664634436No Hit
CGATGTTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG48920.22364287360063195No Hit
CGATGTTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA47810.21856839302629222No Hit
CGATGTTCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTCA47390.21664831929546097No Hit
CGATGTTGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGA45590.20841943187761267No Hit
CGATGTTGCCAGGGTGGTATTTCAAGGTTGGCTCCACGCAGACTGGCGTC45590.20841943187761267No Hit
CGATGTTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT45100.20617934585830952No Hit
CGATGTTCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGCG44800.2048078646220015No Hit
CGATGTTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC43030.19671612532778404No Hit
CGATGTTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT42990.19653326116294295No Hit
CGATGTTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC42880.19603038470963No Hit
CGATGTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTA42530.1944303232672706No Hit
CGATGTTCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTG42350.1936074345254858No Hit
CGATGTTGAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAG42040.19219023724796747No Hit
CGATGTTCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCAT42020.19209880516554695No Hit
CGATGTTGCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT41930.19168736079465454No Hit
CGATGTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA41700.19063589184681834No Hit
CGATGTTCTCCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTCGCTGA39660.18130981943992364No Hit
CGATGTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCA39180.17911544946183075No Hit
CGATGTTCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCT39150.17897830133819995No Hit
CGATGTTCCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCA38320.1751838699177477No Hit
CGATGTTGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC37790.1727609197336035No Hit
CGATGTTCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGC36960.16896648831315123No Hit
CGATGTTCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAA36570.16718356270595075No Hit
CGATGTTCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAACC36080.16494347668664763No Hit
CGATGTTCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTA35540.16247481046129314No Hit
CGATGTTCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAA34900.15954898382383598No Hit
CGATGTTACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACA34820.15918325549415382No Hit
CGATGTTGCCCTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGGTT33170.15164010869445957No Hit
CGATGTTCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACA33100.1513200964059877No Hit
CGATGTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC33040.1510458001587261No Hit
CGATGTTCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAG32620.14912572642789482No Hit
CGATGTTCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG32550.14880571413942295No Hit
CGATGTTGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT32260.14747994894432517No Hit
CGATGTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT32080.14665706020254035No Hit
CGATGTTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA31740.14510271480139122No Hit
CGATGTTGGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA31680.14482841855412962No Hit
CGATGTTCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGA31500.1440055298123448No Hit
CGATGTTCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC31200.14263404857603673No Hit
CGATGTTTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAAC30830.14094255505125683No Hit
CGATGTTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT30800.14080540692762603No Hit
CGATGTTGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAG30540.13961678985615905No Hit
CGATGTTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG30320.13861103694953317No Hit
CGATGTTCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCC30260.13833674070227153No Hit
CGATGTTGCCTTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAAT30180.1379710123725894No Hit
CGATGTTCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTA30080.1375138519604867No Hit
CGATGTTCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATGAG30010.13719383967201484No Hit
CGATGTTCCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTG29940.13687382738354295No Hit
CGATGTTGGGCCGACTAACCCTGCGTCGATTAACGTTGCGCAGGAAACCT28560.13056501369652596No Hit
CGATGTTCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACAG28400.12983355703716165No Hit
CGATGTTCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAA27890.127502038935438No Hit
CGATGTTGACCCATTATACAAAAGGTACGCAGTCACCCAACAAAGTGGGC27720.12672486623486343No Hit
CGATGTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG27310.12485050854524242No Hit
CGATGTTCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAAC27130.1240276198034576No Hit
CGATGTTCCGCTTAGATGCTTTCAGCGGTTATCTTTTCCGAACATAGCTA27040.12361617543256517No Hit
CGATGTTCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA26600.12160466961931339No Hit
CGATGTTCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGA26560.12142180545447231No Hit
CGATGTTCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT26430.12082749691873881No Hit
CGATGTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT26200.11977602797090266No Hit
CGATGTTACCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTT26180.11968459588848211No Hit
CGATGTTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC26150.11954744776485132No Hit
CGATGTTCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTC26130.11945601568243078No Hit
CGATGTTCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGAGG26130.11945601568243078No Hit
CGATGTTCCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT26110.11936458360001023No Hit
CGATGTTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG25830.11808453444612274No Hit
CGATGTTGCCTCCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTCGCT25430.11625589279771201No Hit
CGATGTTGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGAG25330.11579873238560932No Hit
CGATGTTCACCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTT24690.11287290574815215No Hit
CGATGTTCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCT23980.10962706682222312No Hit
CGATGTTCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACTA23930.10939848661617177No Hit
CGATGTTCCCGGATTTACCTAAGATCTCAGCCTACCACCTTAAACCTGGA23900.10926133849254098No Hit
CGATGTTGGCCGTCCTTTCCAGAACGTTCCGCTAATCTCAATACAACTTA23690.10830130162712533No Hit
CGATGTTGACGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCT23660.10816415350349454No Hit
CGATGTTCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACG23030.10528404290724763No Hit
CGATGTTCTTTCGGCGTGGGTGTTTTTCACACCCATTGTCGTTACTCATG23000.10514689478361683No Hit
CGATGTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCTTTA22780.10414114187699093No Hit
CGATGTTCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACC22320.1020382039813186No Hit
CGATGTTGCCTCTTGCACCTATGTATTCAGTACAAGATAACCATCTTATG22200.10148961148679539No Hit
CGATGTTCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTT22160.10130674732195431No Hit
CGATGTTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT22030.10071243878622081No Hit
CGATGTTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT21970.10043814253895922No Hit
CGATGTTGCCACTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGT21900.10011813025048732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTT2170050.0344.220831
GATGTTG789200.0343.469822
GATGTTC1158450.0343.198062
ATGTTGC444350.0341.89423
TGTTGCC374100.0340.481024
ATGTTCC596100.0340.02253
ATGTTCG207400.0339.92633
ATGTTGT189600.0338.449223
TGTTGTC157400.0338.205084
ATGTTGG129600.0338.078553
GATGTTA157150.0337.206332
GTTGCCC260100.0337.1745
TGTTCCC445450.0336.51914
ATGTTCT283850.0336.30953
GTTGTCC129350.0335.931435
TGTTGGC96600.0333.928444
GTTGGCC80750.0331.755255
GTTCCCG133850.0331.210175
TGTTCTC218000.0328.778534
TGTTCGC111400.0328.740884
Produced by FastQC (version 0.11.2)