FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 phi7.3410000000eab7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 phi7.3410000000eab7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760712
Sequences flagged as poor quality0
Sequence length351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT257663.3870899893783717No Hit
CTCAGATGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG212542.7939614466447225No Hit
CTCAGATGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG180562.3735658172869627No Hit
CTCAGATCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTT157332.068194007719084No Hit
CTCAGATCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT146241.922409532122538No Hit
CTCAGATCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC125131.6449063508923218No Hit
CTCAGATCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATGAG116271.5284365173679395No Hit
CTCAGATGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA109301.4368118289181715No Hit
CTCAGATGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT104541.3742388709524762No Hit
CTCAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA96561.2693371473041046No Hit
CTCAGATGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT83621.0992333498091262No Hit
CTCAGATCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC83331.0954211317818046No Hit
CTCAGATCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT72160.9485850098328934No Hit
CTCAGATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT69290.9108571969418123No Hit
CTCAGATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC67590.8885097119540641No Hit
CTCAGATGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC66390.8727350166685947No Hit
CTCAGATGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG65940.8668195059365437No Hit
CTCAGATCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC60890.8004343299435266No Hit
CTCAGATGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA60510.7954390097697945No Hit
CTCAGATCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT60070.7896549548317892No Hit
CTCAGATCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA59890.7872887505389687No Hit
CTCAGATCTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAG57710.7586313874370327No Hit
CTCAGATGACCCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACA49880.6557015006993449No Hit
CTCAGATCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGAGG49670.6529409290243876No Hit
CTCAGATGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTT49170.6463681393221088No Hit
CTCAGATCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT48750.6408469959721945No Hit
CTCAGATGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA47690.6269126818033632No Hit
CTCAGATCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA43980.5781425822124536No Hit
CTCAGATAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT43280.5689406766292631No Hit
CTCAGATCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATGAGG40870.5372598302642788No Hit
CTCAGATCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTCA40350.5304241289739087No Hit
CTCAGATACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA39800.5231940603014018No Hit
CTCAGATCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC38150.5015038542838814No Hit
CTCAGATCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC38110.5009780311076991No Hit
CTCAGATGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC36620.48139111779490795No Hit
CTCAGATCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA35290.463907497186846No Hit
CTCAGATGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA33640.44221729116932557No Hit
CTCAGATCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA32240.42381348000294466No Hit
CTCAGATCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACT31590.415268853389982No Hit
CTCAGATCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC30660.4030434645437432No Hit
CTCAGATCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA27080.35598229027542616No Hit
CTCAGATGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG25830.3395503160197289No Hit
CTCAGATCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG25270.3321887915531765No Hit
CTCAGATCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA24830.32640473661517105No Hit
CTCAGATGTCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAG23690.3114187760939751No Hit
CTCAGATCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCT23280.30602908853810645No Hit
CTCAGATCCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGAG23180.30471453059765063No Hit
CTCAGATCCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACC22770.2993248430417819No Hit
CTCAGATCCCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATG22670.2980102851013261No Hit
CTCAGATACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACA22060.2899914816645458No Hit
CTCAGATGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG21130.27776609281830705No Hit
CTCAGATACCCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCG20630.27119330311602813No Hit
CTCAGATGCCCCTCTTAAAATAAGTACGCTCGAGTCTCAATTGCAACCGT20490.26935292199939004No Hit
CTCAGATCCCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGA19870.2612026627685642No Hit
CTCAGATGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC19790.2601510164161996No Hit
CTCAGATCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC19280.2534467709198751No Hit
CTCAGATCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAA19080.25081765503896347No Hit
CTCAGATCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCC18330.2409584704855451No Hit
CTCAGATGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC18270.24016973572127165No Hit
CTCAGATGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC18200.2392495451629526No Hit
CTCAGATGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT16830.22124010137870836No Hit
CTCAGATGGCCCCTCTTAAAATAAGTACGCTCGAGTCTCAATTGCAACCG16780.22058282240848048No Hit
CTCAGATGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA16570.21782225073352335No Hit
CTCAGATCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG16470.21650769279306756No Hit
CTCAGATCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTA16450.2162447812049764No Hit
CTCAGATTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA16430.2159818696168852No Hit
CTCAGATGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT16390.2154560464407029No Hit
CTCAGATCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA16170.21256401897170019No Hit
CTCAGATCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAA16130.2120381957955179No Hit
CTCAGATCTTTCGGCGTGGGTGTTTTTCACACCCATTGTCGTTACTCATG15740.2069114198277403No Hit
CTCAGATGCCCCGAGTATGGTGTTCAGTCTGGCGTTCGCGACCGCTCTAA15480.2034935691825553No Hit
CTCAGATGCCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCT15350.20178464385996278No Hit
CTCAGATCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT15070.19810388162668657No Hit
CTCAGATCTTCTCCCGAAGTTACGGTGCCATTTTGCCTAGTTCCTTCACC14700.19324001724700018No Hit
CTCAGATTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT14420.18955925501372398No Hit
CTCAGATCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG14400.18929634342563284No Hit
CTCAGATCCCGTCGTCAGTAAGGATACATCTCGTCCTGCCATAAACGCTG13740.18062026101862466No Hit
CTCAGATCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACC13640.17930570307816887No Hit
CTCAGATGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA13420.17641367560916615No Hit
CTCAGATCCGGCTTCTTACTACAAGTTCCTGATTCCGATCGAGAACTTCG13340.17536202925680153No Hit
CTCAGATGCCCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCG13270.17444183869848248No Hit
CTCAGATCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG13150.17286436916993553No Hit
CTCAGATCCTGGATTGGCGGTGTTGTTCCCAATAATAATAAAGGAATACG12600.16563430049742872No Hit
CTCAGATCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATGAGGC12590.16550284470338314No Hit
CTCAGATGCCTGCTGTTCAAGGTATGGTGGGTTCCGACGAAGGTGCTAGT12090.15893005500110421No Hit
CTCAGATCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTC12020.1580098644427852No Hit
CTCAGATACCCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACAT11960.1572211296785117No Hit
CTCAGATCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGAGGAG11760.15459201379760013No Hit
CTCAGATCTGCTGTTCAAGGTATGGTGGGTTCCGACGAAGGTGCTAGTGC11540.1516999863285974No Hit
CTCAGATACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCAC11500.1511741631524151No Hit
CTCAGATGTGGCATTCTGCAAACCAGCGCATGCGCGGTTCTTTCTCCATC11170.14683612194891102No Hit
CTCAGATGCCAGGGTGGTATTTCAAGGTTGGCTCCACGCAGACTGGCGTC11130.14631029877272872No Hit
CTCAGATCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT11010.14473282924418177No Hit
CTCAGATGCTCTACCCCCTACAGTGATACATGAGGCGCTACCTAAATAGC10900.1432868155096804No Hit
CTCAGATCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTA10850.1426295365394525No Hit
CTCAGATTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT10590.13921168589426747No Hit
CTCAGATTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAAC10250.13474218889671782No Hit
CTCAGATCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC10180.13382199833839875No Hit
CTCAGATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTA9800.12882667816466678No Hit
CTCAGATCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGAGGA9800.12882667816466678No Hit
CTCAGATCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCCC9790.1286952223706212No Hit
CTCAGATGCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT9630.12659192966589194No Hit
CTCAGATTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG9590.12606610648970965No Hit
CTCAGATGCCCGCACACGCCCGCTACGGCGGTATTGTCTACCCGGCGACA9530.12527737172543618No Hit
CTCAGATCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC9470.1244886369611627No Hit
CTCAGATCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAG8890.11686420090651917No Hit
CTCAGATGGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC8800.11568109876010896No Hit
CTCAGATGCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTG8760.11515527558392663No Hit
CTCAGATGGTCTTTCGGCGTGGGTGTTTTTCACACCCATTGTCGTTACTC8710.11449799661369875No Hit
CTCAGATCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACA8670.11397217343751644No Hit
CTCAGATGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGC8590.1129205270851518No Hit
CTCAGATCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTC8500.11173742493874161No Hit
CTCAGATCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTCG8330.10950267643996678No Hit
CTCAGATGGGCTCGCTCGGGTTCAACGTCGCTGTCGACTCCGTTCGTCCG8230.10818811849951097No Hit
CTCAGATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAGATCTCAGC8220.1080566627054654No Hit
CTCAGATGTGCTCTACCCCCTACAGTGATACATGAGGCGCTACCTAAATA8210.10792520691141982No Hit
CTCAGATGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAG8010.10529609103050826No Hit
CTCAGATGCCCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACAT7980.10490172364837151No Hit
CTCAGATGCCGCCATGAGTAAATGGAAGTTGTATATCGCCGGCGTTGCGC7790.10240406356150553No Hit
CTCAGATCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGA7660.10069513823891302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGGC20600.0339.975746
CTCAGAT762750.0339.93411
AGATCCC117050.0337.926124
TCAGATG294600.0337.739322
CAGATCC168350.0336.085543
CAGATGC153950.0334.57943
ATGGCCT29850.0334.02016
AGATGCG36300.0332.169434
GATCCCG23650.0331.141665
AGATGCC101700.0330.75224
TCAGATC381750.0330.178772
CAGATGG66600.0329.459443
ATGTCCC25000.0328.446
GATGCCC57850.0328.003455
GATGGGC7000.0327.755
ATGCCCC36850.0324.87116
AGATGGC51050.0321.346744
AGATGTC49000.0318.948974
ATGGGCT3050.0316.721286
GATCCCT46450.0315.290625
Produced by FastQC (version 0.11.2)