##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AGWYV l01n01 phi6.3410000000ea9e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3130664 Sequences flagged as poor quality 0 Sequence length 351 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09262028758116 33.0 32.0 34.0 32.0 34.0 2 32.72814169773569 33.0 32.0 34.0 32.0 34.0 3 32.830160630460504 33.0 32.0 34.0 32.0 34.0 4 32.70965137108294 33.0 32.0 34.0 32.0 34.0 5 32.34242671842139 33.0 32.0 34.0 32.0 34.0 6 35.77218027868848 37.0 37.0 37.0 35.0 37.0 7 36.713722073017095 37.0 37.0 37.0 37.0 37.0 8 36.408460952692465 37.0 37.0 37.0 37.0 37.0 9 35.56713016791326 37.0 35.0 37.0 33.0 37.0 10-19 36.13746604554177 37.5 36.5 37.7 33.7 37.7 20-29 36.901522807941056 38.0 37.8 38.7 34.4 38.7 30-39 36.933523271740434 38.0 37.4 39.0 34.0 39.0 40-49 37.061346027551984 38.6 37.9 39.0 34.7 39.0 50-59 37.03154918573185 38.4 37.7 39.0 34.8 39.0 60-69 36.822223049167846 38.0 37.3 39.0 33.9 39.0 70-79 36.84052153153452 38.0 37.2 39.0 34.1 39.0 80-89 36.80061041363749 38.0 37.0 39.0 33.8 39.0 90-99 36.80713858146387 38.3 37.5 39.0 33.8 39.0 100-109 36.754944222695244 38.1 37.3 39.0 33.4 39.0 110-119 36.42982558971515 38.0 37.0 39.0 32.4 39.0 120-129 36.22985248496805 38.4 37.0 39.0 31.2 39.0 130-139 35.72275312840982 38.0 37.0 39.0 28.1 39.0 140-149 34.562395197951616 38.0 34.4 39.0 18.8 39.0 150-159 34.35832983034909 38.0 34.6 39.0 15.5 39.0 160-169 33.636561029864595 38.0 33.7 39.0 15.0 39.0 170-179 32.4955318105041 37.5 29.5 38.5 14.9 39.0 180-189 31.652980549813076 37.0 25.8 38.0 14.2 38.8 190-199 30.553850780537296 37.0 24.0 38.0 14.0 38.0 200-209 29.31344414475651 36.3 15.2 37.1 13.8 38.0 210-219 28.569513368410025 35.2 15.0 37.0 13.6 37.1 220-229 27.864874448359842 33.3 14.6 37.0 13.4 37.0 230-239 26.987566822884855 32.4 14.0 37.0 13.0 37.0 240-249 26.19324711946092 28.8 14.0 37.0 13.0 37.0 250-259 25.31703737609657 25.7 14.0 37.0 13.0 37.0 260-269 24.363498158857034 24.2 14.0 37.0 13.0 37.0 270-279 23.589518038345858 24.0 14.0 36.1 13.0 37.0 280-289 23.03893487132442 19.9 14.0 34.1 13.0 37.0 290-299 22.221554692550846 15.0 14.0 32.8 13.0 37.0 300-309 21.333023154193487 15.0 14.0 30.1 13.0 37.0 310-319 20.36755892679636 15.0 14.0 26.0 13.0 36.4 320-329 19.56760955503369 14.2 14.0 25.3 13.0 33.8 330-339 18.699583315232807 14.0 14.0 24.2 13.0 32.1 340-349 18.409585187040193 14.0 14.0 24.1 13.0 26.8 350-351 18.110455002517035 14.0 14.0 24.0 13.0 26.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.310752068173052 1101 2 0.1290233645418155 1101 3 0.22151232805025245 1101 4 0.18321430738798483 1101 5 0.3348022542310005 1101 6 0.24056681274752378 1101 7 0.13229641687365046 1101 8 0.1052454916586214 1101 9 0.17997460719178093 1101 10-19 0.1477979698564269 1101 20-29 0.18109501303387532 1101 30-39 0.13583197702561733 1101 40-49 0.15609207105040213 1101 50-59 0.16765529594950124 1101 60-69 0.23854186780991427 1101 70-79 0.2260990782266532 1101 80-89 0.19486205617081964 1101 90-99 0.3038989785105102 1101 100-109 0.3164080634062074 1101 110-119 0.2414541680536857 1101 120-129 0.20340145214002092 1101 130-139 0.16054710568581498 1101 140-149 0.5625892121716589 1101 150-159 0.30282173384931355 1101 160-169 0.2829918561319644 1101 170-179 0.35115637802053357 1101 180-189 0.06516375212403602 1101 190-199 0.2125393198734038 1101 200-209 0.1593492029347452 1101 210-219 0.07363977245832842 1101 220-229 0.1577018381976636 1101 230-239 0.10913946268929564 1101 240-249 0.22417233135220727 1101 250-259 0.10361248542792012 1101 260-269 0.15977994666445383 1101 270-279 0.1664915937592255 1101 280-289 0.18019654117511053 1101 290-299 0.07443690565558825 1101 300-309 0.1491489582062755 1101 310-319 0.24332391833862843 1101 320-329 0.16000483640696928 1101 330-339 0.1259834830660509 1101 340-349 0.1012498122011607 1101 350-351 0.0495307563037386 1102 1 -0.36803227717603093 1102 2 0.048246923506710004 1102 3 -0.4876144339828272 1102 4 -8.423571710736155E-4 1102 5 -0.1296170514165027 1102 6 -0.015784777090992463 1102 7 0.06869724801373422 1102 8 0.04392735409410875 1102 9 0.08589007249166514 1102 10-19 0.034911889212771996 1102 20-29 0.03611997813570156 1102 30-39 0.1665816077257105 1102 40-49 0.11532382873787839 1102 50-59 0.03567242642248658 1102 60-69 0.1057220484013115 1102 70-79 0.14154282458867584 1102 80-89 -0.0034742847303306235 1102 90-99 0.06816739265457272 1102 100-109 -0.013136799146010958 1102 110-119 -0.8774986760368506 1102 120-129 -0.21258993196892106 1102 130-139 -0.17428643807031818 1102 140-149 0.5150590189560234 1102 150-159 0.1530585833335536 1102 160-169 -0.09947322092320121 1102 170-179 0.24204814570833832 1102 180-189 -0.04368603638189228 1102 190-199 -0.08627558846266226 1102 200-209 0.05501152177010482 1102 210-219 -0.44710687356229073 1102 220-229 -0.222196911208691 1102 230-239 -0.0032176844051470255 1102 240-249 0.17791730487169133 1102 250-259 -0.005893009522893067 1102 260-269 0.1858340653251318 1102 270-279 0.02282548827008668 1102 280-289 0.25926431793842397 1102 290-299 0.17242959473866293 1102 300-309 0.31825198182222536 1102 310-319 0.5294906071994987 1102 320-329 -0.1197243004133739 1102 330-339 0.11287300973968328 1102 340-349 0.1497091072067036 1102 350-351 0.05633589444380149 1103 1 0.2913327705752593 1103 2 0.13296983786079153 1103 3 0.060754661172964575 1103 4 0.13213817461831923 1103 5 0.3774959486071765 1103 6 0.4029357987936635 1103 7 0.12646535374208412 1103 8 0.10139464825239486 1103 9 0.1532147116584497 1103 10-19 0.22203636918536773 1103 20-29 -0.025999419600481133 1103 30-39 0.058723998089938334 1103 40-49 -0.15123746659869397 1103 50-59 -0.738180061979179 1103 60-69 0.10385275686422801 1103 70-79 -0.08332523782436141 1103 80-89 -0.4969149072450989 1103 90-99 0.10429361568522211 1103 100-109 -0.02283223404700152 1103 110-119 -0.10629167204933765 1103 120-129 0.05676651539602062 1103 130-139 0.07953923175004718 1103 140-149 0.6931890219783483 1103 150-159 0.11199399338744342 1103 160-169 -0.034683402527143414 1103 170-179 0.299039692259754 1103 180-189 0.016149277709782695 1103 190-199 -0.06637137517864389 1103 200-209 0.12851306078661295 1103 210-219 0.14513091771921793 1103 220-229 0.05984404473643323 1103 230-239 -0.0025489740731217125 1103 240-249 0.09273859789626115 1103 250-259 -0.10358274797364686 1103 260-269 -0.20760534547527953 1103 270-279 0.037885268963712804 1103 280-289 -0.09522531006527046 1103 290-299 0.1474577664009864 1103 300-309 0.2513431737731011 1103 310-319 0.2501918304738844 1103 320-329 0.17506734669641233 1103 330-339 0.18072099808493647 1103 340-349 0.15270560368089647 1103 350-351 -0.05810006297070913 2112 1 -0.31474987820973155 2112 2 0.11597194856879156 2112 3 0.21298580320460303 2112 4 0.3090933600460488 2112 5 0.12281746429970042 2112 6 0.2756450664037189 2112 7 0.08027834816692803 2112 8 0.04539531557808374 2112 9 0.21300249544824368 2112 10-19 0.09471978207488974 2112 20-29 0.18559206004483997 2112 30-39 0.08496693950222323 2112 40-49 0.09899384724442228 2112 50-59 0.03983979677021665 2112 60-69 0.0207659796240236 2112 70-79 -0.6054470596235006 2112 80-89 -0.6055052738509374 2112 90-99 -0.08759920467054627 2112 100-109 -0.021446483276534423 2112 110-119 0.17312254464393106 2112 120-129 -0.15010203804273914 2112 130-139 -0.02694718310906552 2112 140-149 0.4989167464558406 2112 150-159 -0.7049989125420453 2112 160-169 -0.11594742943929504 2112 170-179 0.4003463143052386 2112 180-189 -0.06016023110032975 2112 190-199 0.10164031464106316 2112 200-209 0.326040329424206 2112 210-219 0.23670489666525896 2112 220-229 0.08076930591891696 2112 230-239 0.2666191289207944 2112 240-249 0.3406430209209823 2112 250-259 0.18025319365693093 2112 260-269 -0.7427917575953771 2112 270-279 0.2149792622854747 2112 280-289 0.28914042757447334 2112 290-299 0.35734815317598034 2112 300-309 0.35764564827320555 2112 310-319 0.005383912372341371 2112 320-329 0.2682843312972878 2112 330-339 0.25995039876172754 2112 340-349 0.1296993180372752 2112 350-351 0.11794263342349254 1113 1 0.018935618504421825 1113 2 -0.1967466295827407 1113 3 -0.14388484319150763 1113 4 -0.05621126887250938 1113 5 0.2869505167889983 1113 6 0.08962415055069073 1113 7 0.016345937264382826 1113 8 -0.017624729862035338 1113 9 0.09341529874429 1113 10-19 -0.12325526247610696 1113 20-29 0.09768093526151489 1113 30-39 0.03932071169376883 1113 40-49 0.016223987635235915 1113 50-59 0.036303253795438195 1113 60-69 0.133238059828777 1113 70-79 0.16535502069783092 1113 80-89 -0.1719780844078045 1113 90-99 -0.011887965178587478 1113 100-109 0.057522417318566 1113 110-119 0.09223383434759569 1113 120-129 0.13951524217389988 1113 130-139 0.21778388376823443 1113 140-149 0.2922306666750458 1113 150-159 -1.5951798060510214 1113 160-169 -0.12366258242327888 1113 170-179 0.218559322618745 1113 180-189 -0.02725216614430792 1113 190-199 0.21599794055661548 1113 200-209 0.35998811171031875 1113 210-219 0.2556223384690206 1113 220-229 0.09329483253021564 1113 230-239 0.01573963997681105 1113 240-249 0.20662431747004817 1113 250-259 0.30605537413896045 1113 260-269 -0.2567209584295682 1113 270-279 0.43544341107633144 1113 280-289 0.41617875119303704 1113 290-299 0.4650188988461288 1113 300-309 0.44659457337648334 1113 310-319 0.33562896701985423 1113 320-329 0.40190327861489195 1113 330-339 0.4417602180570448 1113 340-349 0.3321693465896445 1113 350-351 0.20708526685848128 2113 1 0.21709873789397705 2113 2 0.21221606402783522 2113 3 0.27738681666804865 2113 4 0.04727346602621907 2113 5 0.25238710445658086 2113 6 -0.012569529911225175 2113 7 0.09633585896486352 2113 8 0.0922770528717507 2113 9 0.17839996915055423 2113 10-19 0.030976372915489492 2113 20-29 0.20201323038811836 2113 30-39 0.12202479773836217 2113 40-49 0.1391139234503811 2113 50-59 0.1421327176495879 2113 60-69 0.21747844696650986 2113 70-79 0.25806243942356133 2113 80-89 0.23742139121056027 2113 90-99 0.1586913231188234 2113 100-109 0.08316389544030756 2113 110-119 0.1929853612055794 2113 120-129 0.18047166523820124 2113 130-139 0.2239167525367165 2113 140-149 0.5047055493334156 2113 150-159 -0.05126893937123356 2113 160-169 0.08524725722537596 2113 170-179 0.24772443654963183 2113 180-189 0.08635351766162813 2113 190-199 0.09874701108589079 2113 200-209 0.0895310920256911 2113 210-219 0.07901125748361437 2113 220-229 -0.028871127814568354 2113 230-239 -0.23214222298181753 2113 240-249 0.018047904531162118 2113 250-259 0.05342694409254278 2113 260-269 -0.04356012469447634 2113 270-279 0.11049320613164682 2113 280-289 0.1692947409600336 2113 290-299 0.2090071112840981 2113 300-309 0.1916361480739397 2113 310-319 0.29602311799050085 2113 320-329 0.21108890742105046 2113 330-339 0.23917123948314156 2113 340-349 0.17886655603138024 2113 350-351 0.08669001010713373 1112 1 -0.1784905502505154 1112 2 0.05963647702019159 1112 3 0.0649827068669353 1112 4 0.18569651464279247 1112 5 0.3297748128761455 1112 6 0.1817133951151746 1112 7 0.09391893729251422 1112 8 0.06323924980000584 1112 9 0.15110239288663507 1112 10-19 0.07076010352788131 1112 20-29 0.03337946439119577 1112 30-39 0.07266056688299471 1112 40-49 0.07702168733830206 1112 50-59 0.12833188346401414 1112 60-69 0.07261785690954525 1112 70-79 0.09618249075482765 1112 80-89 0.11318546071959901 1112 90-99 0.08078108516247084 1112 100-109 0.05532941130566371 1112 110-119 0.06775737313216723 1112 120-129 0.1278306340095554 1112 130-139 -0.007264582572595657 1112 140-149 0.43027821484087525 1112 150-159 0.28152111954099723 1112 160-169 0.048803420804411246 1112 170-179 0.2209126895588227 1112 180-189 0.03544684636997886 1112 190-199 0.13443529109741803 1112 200-209 -0.2062697880474893 1112 210-219 -0.15657327628623108 1112 220-229 -0.11276863919434277 1112 230-239 -0.21539525246371483 1112 240-249 -0.08146127485513333 1112 250-259 -0.115643096166135 1112 260-269 0.1342188410041807 1112 270-279 0.11900334693195802 1112 280-289 0.29258638508929735 1112 290-299 0.13719653373984997 1112 300-309 0.23778386576790567 1112 310-319 0.2351854168795704 1112 320-329 0.2781064331470766 1112 330-339 0.2756235480910867 1112 340-349 0.23082827398732064 1112 350-351 0.18021138420585103 2114 1 0.1308506421214375 2114 2 0.1517414320495547 2114 3 0.11611591304501445 2114 4 0.24427204454496376 2114 5 0.29179757354371105 2114 6 0.28745810603184907 2114 7 0.11025283056996216 2114 8 0.11356126916660259 2114 9 0.06724614624709346 2114 10-19 0.09957483039769954 2114 20-29 0.16222318536431146 2114 30-39 0.10375722497372664 2114 40-49 0.06558781589182416 2114 50-59 0.02042870574636879 2114 60-69 0.09761976273503592 2114 70-79 0.14779059574127018 2114 80-89 0.14387941337329124 2114 90-99 0.22864039882809806 2114 100-109 0.1817608958769199 2114 110-119 0.22494564330239086 2114 120-129 0.08932665691420993 2114 130-139 -0.09410706818326986 2114 140-149 0.48504717734521563 2114 150-159 0.20961956586729258 2114 160-169 0.2183767246823507 2114 170-179 0.3442632309986635 2114 180-189 -0.062324967189173464 2114 190-199 -0.0014210241919272448 2114 200-209 -0.5570027015535786 2114 210-219 0.07727586150728527 2114 220-229 0.21545699801700025 2114 230-239 -0.008975591192879051 2114 240-249 -0.4245672359131518 2114 250-259 -0.05986155320914577 2114 260-269 0.042512110801091296 2114 270-279 0.2756825838066952 2114 280-289 0.35760057021025915 2114 290-299 0.30939694707514676 2114 300-309 0.3770472892685568 2114 310-319 0.3282966018370175 2114 320-329 0.21963913442337457 2114 330-339 0.27732295063734114 2114 340-349 0.18403594031653014 2114 350-351 0.0537272831499962 1114 1 0.1984619689508662 1114 2 0.1799481835422725 1114 3 0.21671547403413882 1114 4 0.03965123660871939 1114 5 -0.018151312795886554 1114 6 0.06859842824329121 1114 7 0.051634613487969716 1114 8 0.04670529232830489 1114 9 0.016876620578308632 1114 10-19 -0.016575288408859024 1114 20-29 0.16773497919979263 1114 30-39 0.17373831930907357 1114 40-49 0.19222241120846206 1114 50-59 0.011722041504945935 1114 60-69 0.11592335349641303 1114 70-79 0.21308496992131154 1114 80-89 0.1935626630283096 1114 90-99 0.2984582195859744 1114 100-109 0.06587136645767089 1114 110-119 0.12108691709222796 1114 120-129 0.0965396228218296 1114 130-139 -0.12077971030674917 1114 140-149 0.288552858304719 1114 150-159 -0.062540296208212 1114 160-169 0.17762805400234072 1114 170-179 0.2712622208946627 1114 180-189 0.016118945826633535 1114 190-199 0.08596978653867993 1114 200-209 -0.07671535882666447 1114 210-219 0.09521440024297334 1114 220-229 0.17003402045623872 1114 230-239 -0.18611944831715377 1114 240-249 -0.14221712476389925 1114 250-259 -0.0030934322582680807 1114 260-269 0.29153701774772145 1114 270-279 0.3157222411422218 1114 280-289 0.20653272737637707 1114 290-299 0.14759513070988106 1114 300-309 0.3736575637970958 1114 310-319 0.3840556732752667 1114 320-329 0.11101449284442921 1114 330-339 0.21461456560530934 1114 340-349 0.11146787195276886 1114 350-351 -0.06850796052045993 1109 1 0.372650607829101 1109 2 0.11244749320953673 1109 3 0.2602536549093486 1109 4 0.22226165316950386 1109 5 0.4319956422177924 1109 6 0.1591687542640372 1109 7 0.08249446436264662 1109 8 0.030607813353242364 1109 9 0.1026785665242329 1109 10-19 0.05715815248564837 1109 20-29 0.16658436467650972 1109 30-39 0.22839762029678923 1109 40-49 0.2910871775800743 1109 50-59 0.22735548489217194 1109 60-69 0.12238495781834047 1109 70-79 0.2649069628186922 1109 80-89 0.2311711015950877 1109 90-99 0.22902151806364657 1109 100-109 0.08418940191679525 1109 110-119 0.26519921351448517 1109 120-129 0.20511021896454196 1109 130-139 0.221875364234684 1109 140-149 0.557487954333169 1109 150-159 -0.03520442147519276 1109 160-169 0.22928282517148801 1109 170-179 0.41730068624705297 1109 180-189 0.19599817952934728 1109 190-199 0.18377306434651786 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2105 110-119 -0.102059555841997 2105 120-129 -0.21449753793200443 2105 130-139 -0.3740110617530803 2105 140-149 -2.7932694398662434 2105 150-159 -0.04582701944710266 2105 160-169 -0.18826453873250415 2105 170-179 0.2061161025671865 2105 180-189 -0.09695757732951904 2105 190-199 0.1803895441328649 2105 200-209 0.25309956959170066 2105 210-219 0.10580607442004819 2105 220-229 -0.21865789536902724 2105 230-239 -0.12176228618136875 2105 240-249 0.1410651325694161 2105 250-259 -0.02117671872283111 2105 260-269 -0.0062805021761072055 2105 270-279 -0.3162632734698718 2105 280-289 -0.13892742925738943 2105 290-299 -0.21086996218323506 2105 300-309 -0.2735879773561436 2105 310-319 -0.17829359386200494 2105 320-329 -0.17843847361997334 2105 330-339 -0.21209364722398405 2105 340-349 -0.1474610464036772 2105 350-351 0.013472417379507107 2104 1 0.06596127248528205 2104 2 -0.3087196538654666 2104 3 -0.13157603969141007 2104 4 -0.2742633025002519 2104 5 -0.3826281173981556 2104 6 -0.19071697769303597 2104 7 -0.09765483926608454 2104 8 -0.08296410415201905 2104 9 -0.07936416516798062 2104 10-19 -0.02697235758439831 2104 20-29 -0.07751175510962582 2104 30-39 -0.057680314333303784 2104 40-49 0.10285867752321565 2104 50-59 0.1740853780918883 2104 60-69 -0.0928521075226385 2104 70-79 0.04098347151867898 2104 80-89 0.09412103291155205 2104 90-99 -0.013736181164830441 2104 100-109 0.08067345249470748 2104 110-119 0.1252193027245312 2104 120-129 0.07438709660490872 2104 130-139 0.18949465316594427 2104 140-149 -2.363060267712129 2104 150-159 0.5104823061614141 2104 160-169 0.1870947338940283 2104 170-179 0.605536680160597 2104 180-189 0.29960081349248213 2104 190-199 0.550212028641738 2104 200-209 0.39981207379204875 2104 210-219 0.43557037587759595 2104 220-229 0.09989680223613462 2104 230-239 0.25133694523958283 2104 240-249 0.25125388891714096 2104 250-259 0.23619725506338796 2104 260-269 0.26145284771352806 2104 270-279 -0.0937821947615447 2104 280-289 -0.08423671347514627 2104 290-299 0.0037729708815632534 2104 300-309 -0.13209788399639422 2104 310-319 -0.0738574012446982 2104 320-329 -0.05370448907215675 2104 330-339 -0.09129840382004417 2104 340-349 -0.08240433744401443 2104 350-351 0.0723989546056778 2111 1 0.06466972396260218 2111 2 -0.12792935934323424 2111 3 -0.10212753590006685 2111 4 -0.11638712173397181 2111 5 0.12397414765336379 2111 6 -0.02003226792024293 2111 7 -0.014456480328306043 2111 8 -0.024527244182714014 2111 9 6.958587717065257E-4 2111 10-19 -0.0759716773646062 2111 20-29 -0.0291975012600858 2111 30-39 -0.22733289930184952 2111 40-49 -0.22318444635391188 2111 50-59 0.15079977970975023 2111 60-69 -0.2994091515930677 2111 70-79 -0.10328698082848575 2111 80-89 -0.07398279060731028 2111 90-99 0.13168129363818792 2111 100-109 -0.13268451941504367 2111 110-119 -0.15588328672456697 2111 120-129 -0.20888354441702006 2111 130-139 -0.10963064634548658 2111 140-149 -2.5705862262770864 2111 150-159 0.17261118780812268 2111 160-169 -0.06284643292737968 2111 170-179 0.17784839906607885 2111 180-189 0.058495633870350616 2111 190-199 0.3794368288263854 2111 200-209 -1.2621627664036623 2111 210-219 -0.25327133887718034 2111 220-229 0.26850888552395347 2111 230-239 0.16035577858798078 2111 240-249 0.08463676139617249 2111 250-259 0.17650810558233943 2111 260-269 0.052310132028143386 2111 270-279 -0.10694494027166002 2111 280-289 0.002671311741263338 2111 290-299 -0.07484292001140247 2111 300-309 -0.1893878067845307 2111 310-319 -0.11024103344854197 2111 320-329 -0.05147948580677664 2111 330-339 -0.08908220471172612 2111 340-349 -0.12313947213056053 2111 350-351 0.08893356073073022 2110 1 0.34905697163551963 2110 2 0.1628196239207398 2110 3 0.19790208665028075 2110 4 0.1638134594647198 2110 5 0.08764672849846278 2110 6 0.024111908356793776 2110 7 -0.01957231052264774 2110 8 0.020335967283507728 2110 9 -0.14790087046746692 2110 10-19 -0.010927024878725433 2110 20-29 -0.11283187979681486 2110 30-39 -0.07551133743216809 2110 40-49 0.004419998748858234 2110 50-59 0.09481495157115916 2110 60-69 -0.1514420066823945 2110 70-79 0.0017764478928583571 2110 80-89 -0.04576152083393481 2110 90-99 -0.07612789473804327 2110 100-109 -0.1456199379807117 2110 110-119 -0.0934947959325001 2110 120-129 -0.11790016971646367 2110 130-139 0.010434804313490531 2110 140-149 0.2669417930957252 2110 150-159 0.15545038422414592 2110 160-169 -0.063330415593704 2110 170-179 -2.9042671440749004 2110 180-189 -0.019812867278957924 2110 190-199 0.028670593459690252 2110 200-209 -1.8822872726634827 2110 210-219 -1.0776250962987604 2110 220-229 -0.17555048134122941 2110 230-239 -0.20561899377031878 2110 240-249 -0.22391301237127692 2110 250-259 -0.17722458929392104 2110 260-269 -0.29021331503297176 2110 270-279 -0.2713992089431265 2110 280-289 -0.3714278149654646 2110 290-299 -0.5052743397404456 2110 300-309 -0.5634067170716754 2110 310-319 -0.49355748917935927 2110 320-329 -0.28656946562304597 2110 330-339 -0.29969391963518177 2110 340-349 -0.20676643507880144 2110 350-351 -0.15127414600916822 2109 1 -0.036637432453119345 2109 2 0.028784629233300052 2109 3 0.14124056603687762 2109 4 -0.0640008779158876 2109 5 -0.35754860078666084 2109 6 -0.08338875449519634 2109 7 -0.11475476408254082 2109 8 -0.06533206677755032 2109 9 -0.10559730450592042 2109 10-19 -0.08692706623018154 2109 20-29 -0.20749394221599005 2109 30-39 -0.16271706525297702 2109 40-49 -0.08062526542044424 2109 50-59 -0.02425250951800706 2109 60-69 -0.001063840827960405 2109 70-79 -0.09591153705996192 2109 80-89 -0.13996792832694638 2109 90-99 0.0027812733121734823 2109 100-109 -0.2632894130722647 2109 110-119 -0.18018387712260875 2109 120-129 -0.27142570428361523 2109 130-139 0.03367546038825253 2109 140-149 0.24047146026649102 2109 150-159 -0.08380453623912132 2109 160-169 -0.3154076831639898 2109 170-179 -3.1722656293235687 2109 180-189 -0.27894386217045053 2109 190-199 0.1088531245535691 2109 200-209 0.07073400630488891 2109 210-219 -0.26023623744899993 2109 220-229 -0.2034692616562772 2109 230-239 -0.3077625677242182 2109 240-249 -0.7450198129319254 2109 250-259 -0.5508551565621893 2109 260-269 -0.17741672869744463 2109 270-279 -0.3316920230173963 2109 280-289 -0.46387373501293183 2109 290-299 -0.3123420291099954 2109 300-309 -0.597129585368048 2109 310-319 -0.6758049561272159 2109 320-329 -0.4105598990312309 2109 330-339 -0.3662749608225049 2109 340-349 -0.2168615612248992 2109 350-351 -0.512486839347357 2108 1 0.15880502488056436 2108 2 -0.0841047002686679 2108 3 -0.017328093553238944 2108 4 -0.005714531145983415 2108 5 -0.3040387259384758 2108 6 -0.012941940591346679 2108 7 -0.2225436484495802 2108 8 -0.07974293780601016 2108 9 -0.006070510693128028 2108 10-19 -0.05223193728675568 2108 20-29 -0.2792499834813498 2108 30-39 -0.1528905836190404 2108 40-49 -0.09396902387220507 2108 50-59 -0.05201892185174728 2108 60-69 -0.02676292865810126 2108 70-79 -0.02380290228040849 2108 80-89 -0.10622411604909843 2108 90-99 -0.20435439076926087 2108 100-109 -0.1395111752697602 2108 110-119 0.04235134876432767 2108 120-129 0.007892805246861201 2108 130-139 0.14102707154118121 2108 140-149 0.43976814671800213 2108 150-159 0.22007245402382125 2108 160-169 -0.04284576085159841 2108 170-179 -0.0914546485917711 2108 180-189 0.07119304597590315 2108 190-199 0.11438707472360576 2108 200-209 0.1994493958857042 2108 210-219 -0.2868324427636466 2108 220-229 -0.13917085335383916 2108 230-239 -0.05436629613488719 2108 240-249 -0.03570640750690046 2108 250-259 -0.14120302349613567 2108 260-269 -0.10789465173941437 2108 270-279 -0.2422506162117024 2108 280-289 -0.24708028294452689 2108 290-299 -0.3224827151608274 2108 300-309 -0.346687338016185 2108 310-319 -0.4287510045920797 2108 320-329 -0.3706312459907757 2108 330-339 -0.22811515572807295 2108 340-349 -0.2006226264931712 2108 350-351 0.06618507736580881 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 2.0 15 1.0 16 6.0 17 54.0 18 450.0 19 2940.0 20 12675.0 21 32960.0 22 58197.0 23 105112.0 24 153111.0 25 187325.0 26 223226.0 27 246560.0 28 235243.0 29 201982.0 30 210214.0 31 226119.0 32 224092.0 33 231877.0 34 273458.0 35 285937.0 36 191374.0 37 27749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.015076673830216209 99.86022134601478 0.08285782185504417 0.041844158299964476 2 0.005781521108621047 0.04114143197736966 0.02852430027623533 99.92455274663777 3 0.03535991086874861 99.9031515359042 0.04101366355507969 0.020474889671967353 4 99.79483585590788 0.11396943268265135 0.07937613234764254 0.01181857906182203 5 0.0183028264930379 0.06535354800131857 99.78726557688721 0.12907804861844005 6 99.8307068404658 0.03884160037615023 0.11697199060646558 0.013479568551591612 7 0.07484035335634869 0.06260652692208427 99.73021697633473 0.13233614338683425 8 21.938125586137637 6.2759210186720775 3.5168258235313656 68.26912757165891 9 18.757650134284614 6.260652692208427 27.95863752865207 47.02305964485489 10-19 20.446077892740966 17.9367507979138 27.711456738889893 33.905714570455345 20-29 21.399811669345546 22.991758297920185 23.74868717946097 31.8597428532733 30-39 24.884957312570112 24.495790669327658 23.493297268566668 27.125954749535563 40-49 19.870161729268933 24.318179785502373 29.903359159590426 25.908299325638268 50-59 19.767209128798235 19.592450675000574 29.107767553464697 31.532572642736493 60-69 22.66452739738279 21.92808937656676 27.662313809466614 27.745069416583828 70-79 22.237173966928424 16.229221660325095 28.02175193505276 33.511852437693726 80-89 24.21924550191269 23.502962949712906 22.001121806747705 30.276669741626694 90-99 21.379292060725778 22.377850194080235 24.798090756465722 31.44476698872827 100-109 20.37516902167323 22.449211726229546 26.357287317615345 30.81833193448188 110-119 22.55617802205398 23.468981242891683 24.846411166858605 29.12842956819573 120-129 18.72633409398134 27.408776540695516 24.048128448150297 29.81676091717284 130-139 22.17551682887454 23.547644319149047 26.06890187094181 28.2079369810346 140-149 21.990290238748074 23.965800865247754 26.26905346597399 27.774855430030176 150-159 20.335164723542956 23.742167635002367 25.327068037587942 30.595599603866734 160-169 21.980154622809042 21.314995325591674 30.89837566706305 25.806474384536237 170-179 18.499353843022536 25.18599093086788 28.401392497194045 27.913262728915537 180-189 19.391024898781033 27.228958157205856 26.692975894277737 26.68704104973537 190-199 17.980473403815942 27.8511417100925 21.95852802316595 32.2098568629256 200-209 17.471182544606442 24.519050004515016 26.742026202562247 31.267741248316288 210-219 16.11428046117645 25.753005857002364 25.559347191292936 32.57336649052825 220-229 16.473832126049203 27.11566673796146 27.583692171039033 28.826808964950303 230-239 18.73480034517926 27.69280004313463 26.267792076771464 27.30460753491465 240-249 16.632632344482154 28.28626215684907 26.57254858970457 28.50855690896421 250-259 17.238070657876623 31.242088337198865 25.30007222112377 26.219768783800745 260-269 16.130930340893904 31.14520271615292 24.304355064447687 28.41951187850549 270-279 17.944577676796673 28.094339259224572 25.790248818564837 28.170834245413918 280-289 15.692442281636756 27.306844355114418 27.322706809301838 29.67800655394699 290-299 15.243548426869863 29.907638088650106 25.08407804251547 29.764735441964557 300-309 14.293888274446703 29.906363269755204 26.47124051219191 29.328507943606187 310-319 13.220954599560125 32.61959325874531 23.143094107686757 31.01635803400781 320-329 11.127467604385002 35.872674122787224 23.24915275930858 29.7507055135192 330-339 11.301765431099884 35.90723319925418 22.286217894221835 30.5047834754241 340-349 8.79570097520861 39.26935935465437 22.52725021389236 29.407689456244658 350-351 7.443821413575748 43.60233860969088 18.800535413659116 30.15330456307425 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.75 2 0.25 3 0.25 4 0.5 5 0.75 6 1.0 7 2.0 8 2.0 9 1.5 10 1.0 11 0.75 12 1.25 13 1.5 14 2.25 15 1.5 16 1.5 17 1.75 18 1.75 19 1.5 20 2.0 21 1.75 22 2.0 23 0.75 24 2.25 25 4.25 26 2.75 27 5.75 28 8.5 29 10.25 30 20.25 31 32.25 32 57.75 33 118.5 34 248.75 35 470.0 36 881.0 37 1673.75 38 2979.5 39 5093.0 40 8474.0 41 13594.0 42 20425.75 43 29199.25 44 39019.25 45 49731.25 46 60911.75 47 70437.0 48 76068.75 49 93210.5 50 140123.25 51 122392.5 52 124437.0 53 94019.5 54 52152.25 55 15693.0 56 6508.5 57 3437.75 58 2193.0 59 1608.75 60 1176.25 61 904.5 62 665.0 63 460.0 64 281.25 65 181.0 66 102.25 67 59.0 68 36.5 69 20.75 70 11.25 71 11.25 72 7.5 73 4.25 74 5.75 75 4.75 76 2.5 77 2.0 78 2.0 79 2.75 80 3.25 81 2.75 82 2.25 83 1.0 84 1.25 85 1.75 86 2.5 87 1.5 88 0.75 89 1.25 90 1.5 91 0.75 92 0.75 93 0.75 94 1.0 95 0.75 96 0.75 97 0.0 98 1.25 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-19 0.0 20-29 0.0 30-39 0.0 40-49 0.0 50-59 0.0 60-69 0.0 70-79 0.0 80-89 0.0 90-99 0.0 100-109 1.277684222899679E-5 110-119 6.388421114498395E-6 120-129 0.0 130-139 3.1942105572491974E-6 140-149 0.0 150-159 1.277684222899679E-5 160-169 1.277684222899679E-5 170-179 1.916526334349518E-5 180-189 2.2359473900744378E-5 190-199 1.916526334349518E-5 200-209 2.2359473900744378E-5 210-219 3.513631612974116E-5 220-229 2.555368445799358E-5 230-239 2.555368445799358E-5 240-249 2.555368445799358E-5 250-259 3.194210557249197E-5 260-269 2.555368445799358E-5 270-279 4.1524737244239565E-5 280-289 2.8747895015242774E-5 290-299 3.833052668699036E-5 300-309 4.7913158358737954E-5 310-319 1.5971052786245986E-5 320-329 3.513631612974116E-5 330-339 2.8747895015242774E-5 340-349 1.5971052786245986E-5 350-351 1.5971052786245986E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 351 3130664.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.082268285910495 #Duplication Level Percentage of deduplicated Percentage of total 1 81.5756108272502 4.961647506383607 2 6.951040158158907 0.8455618221612039 3 2.7188753830179055 0.4961078854641768 4 1.631253144438418 0.39686877066838255 5 1.0269246040519329 0.3123015475623132 6 0.7721017748013704 0.28176780830217496 7 0.5491671462625458 0.2338127342163657 8 0.42667129691858235 0.2076103438204955 9 0.3374472598411301 0.18472002900291937 >10 2.7887713021484117 3.3868498411249663 >50 0.43609128486105264 1.8774620978869827 >100 0.5219909286177226 6.909707807559094 >500 0.09226097572145893 4.009511891049065 >1k 0.1182408728638212 15.68931706220655 >5k 0.021739353296040313 9.32767005858323 >10k+ 0.031813687750302896 50.87908279400857 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACAGTGTCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTT 178836 5.712398392162174 No Hit ACAGTGTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT 133650 4.269062409763551 No Hit ACAGTGTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA 74176 2.3693376229451646 No Hit ACAGTGTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA 56242 1.7964879016080935 No Hit ACAGTGTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 50972 1.6281530052410607 No Hit ACAGTGTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 48767 1.5577206624537159 No Hit ACAGTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT 47466 1.516163983103904 No Hit ACAGTGTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG 35979 1.1492450163926886 No Hit ACAGTGTCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC 34845 1.1130226686734828 No Hit ACAGTGTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC 34645 1.1066342475589843 No Hit ACAGTGTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT 33479 1.0693897524614586 No Hit ACAGTGTCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT 31572 1.0084761571347165 No Hit ACAGTGTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT 28569 0.9125540141005232 No Hit ACAGTGTCCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACC 27238 0.8700390715835362 No Hit ACAGTGTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA 27108 0.8658865978591123 No Hit ACAGTGTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG 26890 0.8589232188443091 No Hit ACAGTGTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG 26829 0.856974750404387 No Hit ACAGTGTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT 26415 0.8437507186973755 No Hit ACAGTGTCTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAG 26142 0.835030523876085 No Hit ACAGTGTCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTCA 25987 0.8300794975123488 No Hit ACAGTGTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA 25017 0.7990956551070316 No Hit ACAGTGTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA 22412 0.7158864700906901 No Hit ACAGTGTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC 22072 0.7050261541960428 No Hit ACAGTGTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT 20475 0.6540146115967731 No Hit ACAGTGTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC 20211 0.6455818957256352 No Hit ACAGTGTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA 19933 0.6367019903764825 No Hit ACAGTGTCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACG 19917 0.6361909166873226 No Hit ACAGTGTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC 19271 0.6155563164874928 No Hit ACAGTGTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA 18776 0.5997449742291092 No Hit ACAGTGTCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACT 18659 0.5960077478771276 No Hit ACAGTGTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA 18197 0.5812504951026364 No Hit ACAGTGTCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTC 17847 0.5700707581522642 No Hit ACAGTGTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC 17500 0.5589868475186095 No Hit ACAGTGTCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTC 16905 0.5399812947029767 No Hit ACAGTGTCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTC 16773 0.5357649367674078 No Hit ACAGTGTCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTA 16583 0.5296959367086344 No Hit ACAGTGTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT 16494 0.5268530893126826 No Hit ACAGTGTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG 15618 0.4988718048311796 No Hit ACAGTGTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA 13603 0.4345084621026083 No Hit ACAGTGTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA 13551 0.43284747261283874 No Hit ACAGTGTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG 13388 0.4276409094045225 No Hit ACAGTGTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC 13301 0.4248619462197157 No Hit ACAGTGTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC 13299 0.4247980620085707 No Hit ACAGTGTCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCT 13162 0.4204219935451393 No Hit ACAGTGTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC 12872 0.4111587829291166 No Hit ACAGTGTCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCCC 12457 0.3979028091165324 No Hit ACAGTGTCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGA 12158 0.38835211955035737 No Hit ACAGTGTCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTGCC 12131 0.3874896826999001 No Hit ACAGTGTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT 11999 0.3832733247643311 No Hit ACAGTGTGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTT 11747 0.3752239141600632 No Hit ACAGTGTACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACA 10890 0.34784952968443755 No Hit ACAGTGTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG 10852 0.34663572967268286 No Hit ACAGTGTCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAACC 10849 0.3465399033559654 No Hit ACAGTGTCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGT 10805 0.34513445071077575 No Hit ACAGTGTCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCAT 10727 0.34264296647612136 No Hit ACAGTGTCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAAG 10436 0.3333478137545262 No Hit ACAGTGTCTCCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTCGCTGA 10335 0.3301216610917045 No Hit ACAGTGTCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG 10319 0.3296105874025446 No Hit ACAGTGTCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCA 10164 0.3246595610388084 No Hit ACAGTGTACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCAC 10139 0.3238610083994961 No Hit ACAGTGTCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT 9302 0.2971254660353203 No Hit ACAGTGTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA 9294 0.29686992919074034 No Hit ACAGTGTCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACCT 9239 0.2951131133842533 No Hit ACAGTGTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG 9009 0.28776642910258016 No Hit ACAGTGTGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA 8642 0.2760436763574756 No Hit ACAGTGTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT 8510 0.27182731842190666 No Hit ACAGTGTCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTAC 8271 0.2641931551900811 No Hit ACAGTGTGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT 8229 0.2628515867560364 No Hit ACAGTGTCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGCG 8217 0.2624682814891665 No Hit ACAGTGTAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT 8189 0.2615739025331367 No Hit ACAGTGTCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGAGG 8170 0.2609670025272594 No Hit ACAGTGTCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAAC 8145 0.26016844988794713 No Hit ACAGTGTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG 7753 0.24764714450353026 No Hit ACAGTGTGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC 7721 0.2466249971252105 No Hit ACAGTGTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT 7714 0.24640140238620303 No Hit ACAGTGTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC 7644 0.24416545499612863 No Hit ACAGTGTCGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACAG 7595 0.24260029182307652 No Hit ACAGTGTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA 7561 0.2415142602336118 No Hit ACAGTGTCGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTG 7464 0.23841587599308006 No Hit ACAGTGTCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGC 7237 0.23116501802812436 No Hit ACAGTGTCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACA 7092 0.22653341272011307 No Hit ACAGTGTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC 6962 0.2223809389956891 No Hit ACAGTGTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG 6728 0.21490648629172598 No Hit ACAGTGTCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCC 6726 0.21484260208058098 No Hit ACAGTGTCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTT 6442 0.20577104409799327 No Hit ACAGTGTCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAG 6312 0.2016185703735693 No Hit ACAGTGTCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTA 6240 0.19931873877234987 No Hit ACAGTGTGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGA 6134 0.19593287558166572 No Hit ACAGTGTCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATGAG 6102 0.194910728203346 No Hit ACAGTGTCCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTG 6024 0.19241924396869164 No Hit ACAGTGTCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAA 5976 0.190886022901212 No Hit ACAGTGTCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGAGGAG 5827 0.1861266491709107 No Hit ACAGTGTCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTG 5823 0.18599888074862073 No Hit ACAGTGTCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGATT 5780 0.18462537020900358 No Hit ACAGTGTCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCATCG 5581 0.1782688912000777 No Hit ACAGTGTTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAAC 5474 0.17485108590382104 No Hit ACAGTGTCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCC 5307 0.1695167542732149 No Hit ACAGTGTGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACAGC 5301 0.16932510163977993 No Hit ACAGTGTCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGAGGCA 5232 0.167121096355278 No Hit ACAGTGTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT 5157 0.16472543843734108 No Hit ACAGTGTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC 5105 0.1630644489475715 No Hit ACAGTGTCCCGCTTAGATGCTTTCAGCGGTTATCTTTTCCGAACATAGCT 4997 0.15961470154574237 No Hit ACAGTGTCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAG 4864 0.15536640150460093 No Hit ACAGTGTCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACAG 4706 0.15031954882414722 No Hit ACAGTGTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA 4660 0.14885021196781256 No Hit ACAGTGTGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGC 4635 0.14805165932850028 No Hit ACAGTGTCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAACCA 4634 0.14801971722292778 No Hit ACAGTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC 4613 0.14734893300590546 No Hit ACAGTGTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT 4608 0.147189222478043 No Hit ACAGTGTGCCAGGGTGGTATTTCAAGGTTGGCTCCACGCAGACTGGCGTC 4588 0.14655038036659315 No Hit ACAGTGTCTTCTCCCGAAGTTACGGTGCCATTTTGCCTAGTTCCTTCACC 4559 0.1456240593049909 No Hit ACAGTGTCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGTT 4549 0.14530463824926595 No Hit ACAGTGTCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAA 4535 0.1448574487712511 No Hit ACAGTGTCGCTTAGATGCTTTCAGCGGTTATCTTTTCCGAACATAGCTAC 4522 0.14444220139880867 No Hit ACAGTGTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT 4472 0.1428450961201841 No Hit ACAGTGTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA 4098 0.1308987486360721 No Hit ACAGTGTCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTC 4072 0.1300682538911873 No Hit ACAGTGTCCCGAAGTTACGGTGCCATTTTGCCTAGTTCCTTCACCCGAGT 4049 0.12933358546301998 No Hit ACAGTGTAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGG 3980 0.12712958017851803 No Hit ACAGTGTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCT 3970 0.12681015912279311 No Hit ACAGTGTCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA 3950 0.12617131701134326 No Hit ACAGTGTCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTCG 3915 0.12505334331630605 No Hit ACAGTGTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC 3909 0.1248616906828711 No Hit ACAGTGTCTCCTCTACCGCATCATCAAAAGATGATACCCGTAGCTTCGGT 3830 0.12233826434264423 No Hit ACAGTGTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCC 3822 0.12208272749806431 No Hit ACAGTGTCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTA 3821 0.12205078539249183 No Hit ACAGTGTGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGAG 3773 0.12051756432501219 No Hit ACAGTGTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC 3764 0.12023008537485977 No Hit ACAGTGTATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCAT 3753 0.11987872221356237 No Hit ACAGTGTGCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT 3674 0.11735529587333549 No Hit ACAGTGTCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGT 3646 0.11646091691730572 No Hit ACAGTGTCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTGC 3572 0.11409720110494131 No Hit ACAGTGTCTTTCGGCGTGGGTGTTTTTCACACCCATTGTCGTTACTCATG 3501 0.1118293116092944 No Hit ACAGTGTCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACC 3363 0.10742130104029049 No Hit ACAGTGTCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGACAC 3350 0.1070060536678481 No Hit ACAGTGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGT 3342 0.10675051682326815 No Hit ACAGTGTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC 3285 0.10492981680563614 No Hit ACAGTGTGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCT 3268 0.10438680101090377 No Hit ACAGTGTCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACTA 3267 0.10435485890533126 No Hit ACAGTGTGCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTG 3256 0.10400349574403386 No Hit ACAGTGTCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGC 3255 0.10397155363846136 No Hit ACAGTGTACCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTT 3252 0.10387572732174388 No Hit ACAGTGTCGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGG 3237 0.1033965957381565 No Hit ACAGTGTCCCGGATTTACCTAAGATCTCAGCCTACCACCTTAAACCTGGA 3213 0.1026299852044167 No Hit ACAGTGTCTCGCCACTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAG 3201 0.1022466799375468 No Hit ACAGTGTCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATGAGG 3187 0.10179949045953192 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.007346684281673153 0.0 0.0 9 0.007410568492818137 0.0 0.0 10-19 0.30406329136566557 0.0 0.0 20-29 0.49245144161110865 0.0 0.0 30-39 0.5247576871871271 0.0 0.0 40-49 1.0775286009613296 0.0 0.0 50-59 2.706742084107397 0.0 0.0 60-69 4.748909496515756 0.0 0.0 70-79 6.9943660514191235 2.8747895015242774E-5 0.0 80-89 9.452314269432938 3.194210557249197E-5 0.0 90-99 12.610663424755897 4.7913158358737954E-5 0.0 100-109 16.5558392724355 8.624368504572834E-5 0.0 110-119 21.076343548844587 9.582631671747592E-5 0.0 120-129 25.509914190727592 1.149915800609711E-4 0.0 130-139 29.334943002506815 1.5971052786245985E-4 0.0 140-149 32.04507733822601 1.5971052786245985E-4 0.0 150-159 34.493436536146966 1.7568158064870586E-4 2.5553684457993576E-5 160-169 37.33222409048049 1.9165263343495184E-4 3.194210557249197E-5 170-179 40.40188279547086 1.9165263343495184E-4 3.194210557249197E-5 180-189 43.90681337888704 1.9165263343495184E-4 1.022147378319743E-4 190-199 47.25057048600552 1.9165263343495184E-4 1.5332210674796147E-4 200-209 49.46651572957047 1.9165263343495184E-4 1.9165263343495184E-4 210-219 51.13381059098006 1.9165263343495184E-4 2.0442947566394858E-4 220-229 54.22988541727889 1.9165263343495184E-4 2.5234263402268653E-4 230-239 57.322829917231616 1.9165263343495184E-4 3.8330526686990363E-4 240-249 60.058220875826976 1.9165263343495184E-4 4.344126357858907E-4 250-259 64.77967293839262 1.9165263343495184E-4 5.302389525033667E-4 260-269 67.58560484293427 1.9165263343495184E-4 6.388421114498393E-4 270-279 68.77183562336936 1.9165263343495184E-4 8.113294815412959E-4 280-289 69.95738923116629 2.1720631789294535E-4 0.001025341588876992 290-299 71.69694352380198 2.2359473900744378E-4 0.0012425479067699374 300-309 72.84869918969267 2.2359473900744378E-4 0.0014405889613193876 310-319 73.68730722939287 2.2359473900744378E-4 0.0014693368563346305 320-329 74.39460446729512 2.2359473900744378E-4 0.00155238633082311 330-339 74.83443128997554 2.2359473900744378E-4 0.0015651631730521066 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGTCC 93400 0.0 342.96695 3 ACAGTGT 311995 0.0 342.63217 1 CAGTGTC 214010 0.0 341.2666 2 AGTGTCT 67725 0.0 340.87234 3 AGTGTCG 37000 0.0 340.3364 3 CAGTGTG 69550 0.0 339.66608 2 AGTGTGC 41525 0.0 338.51813 3 GTGTGCC 32315 0.0 338.21918 4 TGTCCCG 27530 0.0 337.29153 5 TGTGCCC 19905 0.0 335.55246 5 AGTGTGG 12690 0.0 335.48322 3 TGTGGCC 6170 0.0 333.81543 5 GTCCCGT 15500 0.0 330.0857 6 GTGTGGC 8010 0.0 329.9237 4 GTGCCCG 6625 0.0 329.89673 6 GTGTCCC 71695 0.0 328.20456 4 GTGTCGC 20255 0.0 327.7103 4 AGTGTGT 14080 0.0 325.15137 3 GTGTCCG 9560 0.0 322.2633 4 TGTCCGG 4270 0.0 321.9717 5 >>END_MODULE