FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 phi6.3410000000ea9e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 phi6.3410000000ea9e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130664
Sequences flagged as poor quality0
Sequence length351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGTCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTT1788365.712398392162174No Hit
ACAGTGTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT1336504.269062409763551No Hit
ACAGTGTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA741762.3693376229451646No Hit
ACAGTGTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA562421.7964879016080935No Hit
ACAGTGTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT509721.6281530052410607No Hit
ACAGTGTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA487671.5577206624537159No Hit
ACAGTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT474661.516163983103904No Hit
ACAGTGTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG359791.1492450163926886No Hit
ACAGTGTCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC348451.1130226686734828No Hit
ACAGTGTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC346451.1066342475589843No Hit
ACAGTGTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT334791.0693897524614586No Hit
ACAGTGTCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT315721.0084761571347165No Hit
ACAGTGTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT285690.9125540141005232No Hit
ACAGTGTCCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACC272380.8700390715835362No Hit
ACAGTGTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA271080.8658865978591123No Hit
ACAGTGTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG268900.8589232188443091No Hit
ACAGTGTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG268290.856974750404387No Hit
ACAGTGTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT264150.8437507186973755No Hit
ACAGTGTCTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAG261420.835030523876085No Hit
ACAGTGTCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTCA259870.8300794975123488No Hit
ACAGTGTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA250170.7990956551070316No Hit
ACAGTGTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA224120.7158864700906901No Hit
ACAGTGTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC220720.7050261541960428No Hit
ACAGTGTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT204750.6540146115967731No Hit
ACAGTGTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC202110.6455818957256352No Hit
ACAGTGTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA199330.6367019903764825No Hit
ACAGTGTCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACG199170.6361909166873226No Hit
ACAGTGTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC192710.6155563164874928No Hit
ACAGTGTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA187760.5997449742291092No Hit
ACAGTGTCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACT186590.5960077478771276No Hit
ACAGTGTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA181970.5812504951026364No Hit
ACAGTGTCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTC178470.5700707581522642No Hit
ACAGTGTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC175000.5589868475186095No Hit
ACAGTGTCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTC169050.5399812947029767No Hit
ACAGTGTCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTC167730.5357649367674078No Hit
ACAGTGTCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTA165830.5296959367086344No Hit
ACAGTGTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT164940.5268530893126826No Hit
ACAGTGTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG156180.4988718048311796No Hit
ACAGTGTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA136030.4345084621026083No Hit
ACAGTGTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA135510.43284747261283874No Hit
ACAGTGTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG133880.4276409094045225No Hit
ACAGTGTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC133010.4248619462197157No Hit
ACAGTGTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC132990.4247980620085707No Hit
ACAGTGTCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCT131620.4204219935451393No Hit
ACAGTGTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC128720.4111587829291166No Hit
ACAGTGTCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCCC124570.3979028091165324No Hit
ACAGTGTCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGA121580.38835211955035737No Hit
ACAGTGTCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTGCC121310.3874896826999001No Hit
ACAGTGTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT119990.3832733247643311No Hit
ACAGTGTGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTT117470.3752239141600632No Hit
ACAGTGTACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACA108900.34784952968443755No Hit
ACAGTGTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG108520.34663572967268286No Hit
ACAGTGTCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAACC108490.3465399033559654No Hit
ACAGTGTCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGT108050.34513445071077575No Hit
ACAGTGTCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCAT107270.34264296647612136No Hit
ACAGTGTCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAAG104360.3333478137545262No Hit
ACAGTGTCTCCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTCGCTGA103350.3301216610917045No Hit
ACAGTGTCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG103190.3296105874025446No Hit
ACAGTGTCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCA101640.3246595610388084No Hit
ACAGTGTACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCAC101390.3238610083994961No Hit
ACAGTGTCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT93020.2971254660353203No Hit
ACAGTGTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA92940.29686992919074034No Hit
ACAGTGTCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACCT92390.2951131133842533No Hit
ACAGTGTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG90090.28776642910258016No Hit
ACAGTGTGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA86420.2760436763574756No Hit
ACAGTGTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT85100.27182731842190666No Hit
ACAGTGTCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTAC82710.2641931551900811No Hit
ACAGTGTGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT82290.2628515867560364No Hit
ACAGTGTCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGCG82170.2624682814891665No Hit
ACAGTGTAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT81890.2615739025331367No Hit
ACAGTGTCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGAGG81700.2609670025272594No Hit
ACAGTGTCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAAC81450.26016844988794713No Hit
ACAGTGTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG77530.24764714450353026No Hit
ACAGTGTGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC77210.2466249971252105No Hit
ACAGTGTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT77140.24640140238620303No Hit
ACAGTGTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC76440.24416545499612863No Hit
ACAGTGTCGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACAG75950.24260029182307652No Hit
ACAGTGTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA75610.2415142602336118No Hit
ACAGTGTCGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTG74640.23841587599308006No Hit
ACAGTGTCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGC72370.23116501802812436No Hit
ACAGTGTCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACA70920.22653341272011307No Hit
ACAGTGTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC69620.2223809389956891No Hit
ACAGTGTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG67280.21490648629172598No Hit
ACAGTGTCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCC67260.21484260208058098No Hit
ACAGTGTCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTT64420.20577104409799327No Hit
ACAGTGTCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAG63120.2016185703735693No Hit
ACAGTGTCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTA62400.19931873877234987No Hit
ACAGTGTGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGA61340.19593287558166572No Hit
ACAGTGTCCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATGAG61020.194910728203346No Hit
ACAGTGTCCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTG60240.19241924396869164No Hit
ACAGTGTCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAA59760.190886022901212No Hit
ACAGTGTCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGAGGAG58270.1861266491709107No Hit
ACAGTGTCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTG58230.18599888074862073No Hit
ACAGTGTCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGATT57800.18462537020900358No Hit
ACAGTGTCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCATCG55810.1782688912000777No Hit
ACAGTGTTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAAC54740.17485108590382104No Hit
ACAGTGTCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCC53070.1695167542732149No Hit
ACAGTGTGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACAGC53010.16932510163977993No Hit
ACAGTGTCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGAGGCA52320.167121096355278No Hit
ACAGTGTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT51570.16472543843734108No Hit
ACAGTGTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC51050.1630644489475715No Hit
ACAGTGTCCCGCTTAGATGCTTTCAGCGGTTATCTTTTCCGAACATAGCT49970.15961470154574237No Hit
ACAGTGTCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAG48640.15536640150460093No Hit
ACAGTGTCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACAG47060.15031954882414722No Hit
ACAGTGTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA46600.14885021196781256No Hit
ACAGTGTGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGC46350.14805165932850028No Hit
ACAGTGTCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAACCA46340.14801971722292778No Hit
ACAGTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC46130.14734893300590546No Hit
ACAGTGTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT46080.147189222478043No Hit
ACAGTGTGCCAGGGTGGTATTTCAAGGTTGGCTCCACGCAGACTGGCGTC45880.14655038036659315No Hit
ACAGTGTCTTCTCCCGAAGTTACGGTGCCATTTTGCCTAGTTCCTTCACC45590.1456240593049909No Hit
ACAGTGTCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGTT45490.14530463824926595No Hit
ACAGTGTCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAA45350.1448574487712511No Hit
ACAGTGTCGCTTAGATGCTTTCAGCGGTTATCTTTTCCGAACATAGCTAC45220.14444220139880867No Hit
ACAGTGTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT44720.1428450961201841No Hit
ACAGTGTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA40980.1308987486360721No Hit
ACAGTGTCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTC40720.1300682538911873No Hit
ACAGTGTCCCGAAGTTACGGTGCCATTTTGCCTAGTTCCTTCACCCGAGT40490.12933358546301998No Hit
ACAGTGTAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGG39800.12712958017851803No Hit
ACAGTGTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGTCT39700.12681015912279311No Hit
ACAGTGTCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA39500.12617131701134326No Hit
ACAGTGTCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTCG39150.12505334331630605No Hit
ACAGTGTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC39090.1248616906828711No Hit
ACAGTGTCTCCTCTACCGCATCATCAAAAGATGATACCCGTAGCTTCGGT38300.12233826434264423No Hit
ACAGTGTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCC38220.12208272749806431No Hit
ACAGTGTCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTA38210.12205078539249183No Hit
ACAGTGTGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGAG37730.12051756432501219No Hit
ACAGTGTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC37640.12023008537485977No Hit
ACAGTGTATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCAT37530.11987872221356237No Hit
ACAGTGTGCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT36740.11735529587333549No Hit
ACAGTGTCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAACTGT36460.11646091691730572No Hit
ACAGTGTCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTTGC35720.11409720110494131No Hit
ACAGTGTCTTTCGGCGTGGGTGTTTTTCACACCCATTGTCGTTACTCATG35010.1118293116092944No Hit
ACAGTGTCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACC33630.10742130104029049No Hit
ACAGTGTCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGACAC33500.1070060536678481No Hit
ACAGTGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGT33420.10675051682326815No Hit
ACAGTGTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC32850.10492981680563614No Hit
ACAGTGTGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCT32680.10438680101090377No Hit
ACAGTGTCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACTA32670.10435485890533126No Hit
ACAGTGTGCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTG32560.10400349574403386No Hit
ACAGTGTCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGC32550.10397155363846136No Hit
ACAGTGTACCCTCACCGGCGCACCTTCTCCCGAAGTTACGGTGCCATTTT32520.10387572732174388No Hit
ACAGTGTCGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGG32370.1033965957381565No Hit
ACAGTGTCCCGGATTTACCTAAGATCTCAGCCTACCACCTTAAACCTGGA32130.1026299852044167No Hit
ACAGTGTCTCGCCACTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAG32010.1022466799375468No Hit
ACAGTGTCCTAGCCGAAACAGTGCTCTACCCCCTACAGTGATACATGAGG31870.10179949045953192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTCC934000.0342.966953
ACAGTGT3119950.0342.632171
CAGTGTC2140100.0341.26662
AGTGTCT677250.0340.872343
AGTGTCG370000.0340.33643
CAGTGTG695500.0339.666082
AGTGTGC415250.0338.518133
GTGTGCC323150.0338.219184
TGTCCCG275300.0337.291535
TGTGCCC199050.0335.552465
AGTGTGG126900.0335.483223
TGTGGCC61700.0333.815435
GTCCCGT155000.0330.08576
GTGTGGC80100.0329.92374
GTGCCCG66250.0329.896736
GTGTCCC716950.0328.204564
GTGTCGC202550.0327.71034
AGTGTGT140800.0325.151373
GTGTCCG95600.0322.26334
TGTCCGG42700.0321.97175
Produced by FastQC (version 0.11.2)