FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 phi10.3410000000eb30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 phi10.3410000000eb30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371255
Sequences flagged as poor quality0
Sequence length351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATCTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA588864.294314332491039No Hit
CAGATCTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT460643.359258489485909No Hit
CAGATCTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT443893.2371076130989493No Hit
CAGATCTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC296392.1614506419302026No Hit
CAGATCTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA268371.9571122803563161No Hit
CAGATCTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT263421.921013961662856No Hit
CAGATCTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC216391.5780434711268145No Hit
CAGATCTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG189141.3793204035719104No Hit
CAGATCTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC188281.373048776485774No Hit
CAGATCTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA166651.215310062679808No Hit
CAGATCTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT155621.1348727990052907No Hit
CAGATCTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC141011.028328064437322No Hit
CAGATCTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG138391.009221479593511No Hit
CAGATCTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC120900.8816740868766203No Hit
CAGATCTCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC116750.8514098398911947No Hit
CAGATCTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA107840.7864328662429673No Hit
CAGATCTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG106850.7792132025042753No Hit
CAGATCTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGT99860.7282380009553293No Hit
CAGATCTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA84290.6146923803377199No Hit
CAGATCTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA82010.5980652759698233No Hit
CAGATCTCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCC74970.5467254449391251No Hit
CAGATCTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT74930.5464337413537235No Hit
CAGATCTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC73290.534473894352254No Hit
CAGATCTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTT71930.5245559724485964No Hit
CAGATCTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC71620.5222952696617332No Hit
CAGATCTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA70400.5133983103069816No Hit
CAGATCTCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC69770.5088039788369049No Hit
CAGATCTGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTC69110.503990869677777No Hit
CAGATCTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA65750.4794877685040346No Hit
CAGATCTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC60280.43959730320035295No Hit
CAGATCTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT59930.43704489682808817No Hit
CAGATCTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT55510.40481165064120095No Hit
CAGATCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACT54030.3940186179813383No Hit
CAGATCTCCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCC53730.3918308410908256No Hit
CAGATCTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC53690.39153913750542385No Hit
CAGATCTGTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTAC51740.37731858771709126No Hit
CAGATCTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCG50110.36543166661197224No Hit
CAGATCTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG49930.36411900047766466No Hit
CAGATCTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT48910.3566805590499214No Hit
CAGATCTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC48130.3509923391345884No Hit
CAGATCTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT47350.34530411921925536No Hit
CAGATCTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT47340.3452311933229049No Hit
CAGATCTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCT46170.33669886344990535No Hit
CAGATCTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGC46110.3362613080718029No Hit
CAGATCTGTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCG46020.33560497500464903No Hit
CAGATCTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA45850.33436523476669183No Hit
CAGATCTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC44390.32371805389953No Hit
CAGATCTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT44180.3221866100761711No Hit
CAGATCTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC43880.31999883318565836No Hit
CAGATCTGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGC43870.319925907289308No Hit
CAGATCTGGCCAGATTTTCTTAAAGATCTTCTTCAAGACAGAGATGACCT42290.30840361566594104No Hit
CAGATCTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA41380.3017673590980525No Hit
CAGATCTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC40730.297027175835275No Hit
CAGATCTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT40520.2954957320119161No Hit
CAGATCTACCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT40480.2952040284265144No Hit
CAGATCTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA37030.2700445941856183No Hit
CAGATCTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG35750.2607100794527641No Hit
CAGATCTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG35220.2568450069461916No Hit
CAGATCTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT34480.2514484906162603No Hit
CAGATCTACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTA34190.249333639622098No Hit
CAGATCTCCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT33940.2475104922133374No Hit
CAGATCTGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC33070.24116593923085056No Hit
CAGATCTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCA32910.2399991248892438No Hit
CAGATCTGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC32350.23591527469362006No Hit
CAGATCTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC32230.23504016393741498No Hit
CAGATCTTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTC31930.23285238704690228No Hit
CAGATCTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC30430.22191350259433879No Hit
CAGATCTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCT30150.2198715774965269No Hit
CAGATCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC29470.21491261654469812No Hit
CAGATCTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTCCGAGCGT29130.2124331360687837No Hit
CAGATCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCC28370.20689076794615152No Hit
CAGATCTGAGGGATGTCCACCCGCGGGAAGCGTCAACTCGAGGGAACGAA28060.2046300651592884No Hit
CAGATCTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA28030.20441128747023712No Hit
CAGATCTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG27980.204046657988485No Hit
CAGATCTCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCC27260.1987959934512545No Hit
CAGATCTCGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT27120.1977750309023486No Hit
CAGATCTGATCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGG26580.1938370324994257TruSeq Adapter, Index 19 (97% over 36bp)
CAGATCTCCGTCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAAT26290.19172218150526343No Hit
CAGATCTGCGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCC26280.191649255608913No Hit
CAGATCTCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTC26170.19084707074905835No Hit
CAGATCTCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCT25820.1882946643767935No Hit
CAGATCTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT25800.1881488125840927No Hit
CAGATCTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTA24530.1788872237475889No Hit
CAGATCTCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC24310.17728285402787955No Hit
CAGATCTGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC22890.16692737674611943No Hit
CAGATCTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT22810.16634396957531605No Hit
CAGATCTGCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA22770.16605226598991435No Hit
CAGATCTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCA22700.16554178471546138No Hit
CAGATCTCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT22510.16415619268480333No Hit
CAGATCTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCG22220.16204134169064105No Hit
CAGATCTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT21750.15861382456217116No Hit
CAGATCTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT21690.1581762691840686No Hit
CAGATCTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC21470.1565718994643593No Hit
CAGATCTCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGT21160.15431119667749615No Hit
CAGATCTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGG21120.15401949309209448No Hit
CAGATCTCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCCA20980.15299853054318854No Hit
CAGATCTCACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGC20950.15277975285413728No Hit
CAGATCTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG20390.14869590265851354No Hit
CAGATCTGAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAG20390.14869590265851354No Hit
CAGATCTCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG20240.1476020142132572No Hit
CAGATCTCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC19670.14344523812128304No Hit
CAGATCTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG19600.1429347568468301No Hit
CAGATCTACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCC19540.14249720146872755No Hit
CAGATCTCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGA19380.1413303871271208No Hit
CAGATCTCGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAA19290.14067405405996697No Hit
CAGATCTCACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTC18560.13535046362638606No Hit
CAGATCTCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTG18510.13498583414463394No Hit
CAGATCTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGG18370.13396487159572798No Hit
CAGATCTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCAC18280.1333085385285742No Hit
CAGATCTGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTA18270.13323561263222378No Hit
CAGATCTATCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTC18040.13155831701616402No Hit
CAGATCTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA18020.13141246522346317No Hit
CAGATCTCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGCG17900.1305373544672581No Hit
CAGATCTCCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACC17840.13009979908915556No Hit
CAGATCTCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCT17650.1287142070584975No Hit
CAGATCTCCTTCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCA17590.12827665168039498No Hit
CAGATCTTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTAT17550.12798494809499328No Hit
CAGATCTACGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCC17270.1259430229971814No Hit
CAGATCTCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTC17220.1255783935154293No Hit
CAGATCTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTC17180.1252866899300276No Hit
CAGATCTGACCCATTATACAAAAGGTACGCAGTCACCCAACAAAGTGGGC17110.12477620865557465No Hit
CAGATCTGCCGGTGCTTATTCTGTCGGTAACGTCAAAACAATCACGTATT16970.1237552461066687No Hit
CAGATCTGCCCGGGGATTTCACATCCAACTTAACAAACCACCTACGCGCG16950.12360939431396785No Hit
CAGATCTGTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATG16650.12142161742345517No Hit
CAGATCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGCCT16340.11916091463659202No Hit
CAGATCTGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT16270.11865043336213907No Hit
CAGATCTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGC16220.11828580388038694No Hit
CAGATCTCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTT16130.11762947081323312No Hit
CAGATCTCCCGGGGATTTCACATCCAACTTAACAAACCACCTACGCGCGC15690.1144207313738145No Hit
CAGATCTCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTG15620.11391025009936154No Hit
CAGATCTGCCACTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGT15490.11296221344680601No Hit
CAGATCTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT15470.11281636165410519No Hit
CAGATCTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA15210.11092028834899417No Hit
CAGATCTCGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT15180.1107015106599429No Hit
CAGATCTGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACT15120.11026395528184035No Hit
CAGATCTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCG15030.10960762221468655No Hit
CAGATCTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCCTTTTCACGACG14690.10712814173877215No Hit
CAGATCTCCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT14570.10625303098256707No Hit
CAGATCTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGT14470.10552377201906284No Hit
CAGATCTGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTAC14370.10479451305555859No Hit
CAGATCTCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCAT14350.10464866126285775No Hit
CAGATCTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT13870.10114821823803742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCC353800.0337.101233
ATCTGCC216650.0332.021454
GATCTGG51650.0328.634833
GATCTGC308950.0324.39693
GATCTGT101800.0323.987983
ATCTGGC33200.0323.697054
TGGCCCG5000.0317.399757
TGATCTG73050.0316.6637
GATCTCT109050.0309.250123
TCTGCCC114850.0308.952765
TGCCGCG22400.0308.805577
CTGCCCC79650.0308.399056
AGATCTA124600.0305.266662
ATCTCCC282250.0303.685334
GATCTTG29250.0302.538273
TCTACGG18150.0302.231175
TGCCCGG16200.0298.147927
TCTGGCC25850.0296.953375
GATCTAG37200.0296.310243
AGATCTT131700.0295.751462
Produced by FastQC (version 0.11.2)