FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 nct94.3410000000ead1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 nct94.3410000000ead1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635897
Sequences flagged as poor quality0
Sequence length351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT257944.056317296669114No Hit
TAGCTTTCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTT154472.429166987735435No Hit
TAGCTTTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA130972.059610282797371No Hit
TAGCTTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA127151.9995376609733966No Hit
TAGCTTTCTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAG108061.6993318100258374No Hit
TAGCTTTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT96491.5173841046584589No Hit
TAGCTTTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT94471.4856179538510168No Hit
TAGCTTTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG84391.3271017161584344No Hit
TAGCTTTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC79081.2435976266596636No Hit
TAGCTTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA77551.2195371262956107No Hit
TAGCTTTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA71051.1173193142914655No Hit
TAGCTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC70521.1089846311588196No Hit
TAGCTTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA70421.107412049435679No Hit
TAGCTTTCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC67151.0559886270889782No Hit
TAGCTTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC54380.8551699410439113No Hit
TAGCTTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT53570.8424320290864715No Hit
TAGCTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA49510.7785852111269592No Hit
TAGCTTTCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT34850.5480447305145331No Hit
TAGCTTTCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG33340.5242987464951084No Hit
TAGCTTTCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTA30620.48152452362568154No Hit
TAGCTTTCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT29800.46862935349592777No Hit
TAGCTTTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT27680.435290620965345No Hit
TAGCTTTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT27490.43230271569137774No Hit
TAGCTTTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG25970.40839947349963907No Hit
TAGCTTTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCC24550.3860688130310412No Hit
TAGCTTTGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC24490.3851252639971568No Hit
TAGCTTTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC24460.38465348948021455No Hit
TAGCTTTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC24320.3824518750678176No Hit
TAGCTTTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG22460.35320185501740065No Hit
TAGCTTTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT21550.3388913613368203No Hit
TAGCTTTGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGC21350.33574619789053883No Hit
TAGCTTTCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC20980.3299276455149183No Hit
TAGCTTTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG19930.313415537421941No Hit
TAGCTTTCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGATT19510.30681069418475004No Hit
TAGCTTTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG18830.2961171384673933No Hit
TAGCTTTCCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACC18740.29470181491656666No Hit
TAGCTTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC17520.2755163178942502No Hit
TAGCTTTCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACT17430.2741009943434235No Hit
TAGCTTTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA17280.2717421217587125No Hit
TAGCTTTCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACC17160.26985502369094366No Hit
TAGCTTTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT16860.2651372785215216No Hit
TAGCTTTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC16590.2608913078690417No Hit
TAGCTTTCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCT16180.25444372280416483No Hit
TAGCTTTCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAACC16020.2519275920471397No Hit
TAGCTTTCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTC15880.24972597763474272No Hit
TAGCTTTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA15230.2395041964343282No Hit
TAGCTTTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC15040.23651629116036085No Hit
TAGCTTTCCGCCGTGTGTCTCCCATGCTCGGCACTTGTAGGTATTCGGAG14690.23101225512936843No Hit
TAGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA14000.2201614412396976No Hit
TAGCTTTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC13950.21937515037812727No Hit
TAGCTTTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT13250.20836707831614237No Hit
TAGCTTTCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGA13100.20600820573143133No Hit
TAGCTTTCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGAT12830.20176223507895147No Hit
TAGCTTTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT12420.19531465001407458No Hit
TAGCTTTCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTCG12410.19515739184176054No Hit
TAGCTTTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC12400.19500013366944646No Hit
TAGCTTTGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCCC12370.19452835915250427No Hit
TAGCTTTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG11820.18587915967523044No Hit
TAGCTTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCT11770.18509286881366008No Hit
TAGCTTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA11690.18383480343514752No Hit
TAGCTTTCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTC11490.1806896399888661No Hit
TAGCTTTCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCAT11480.18053238181655204No Hit
TAGCTTTCTCGCAGTCAAGCGCGCTTTTGCCTTTATACTCTACGACCGAT11300.1777017347148988No Hit
TAGCTTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC11280.17738721837027066No Hit
TAGCTTTCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAA11090.17439931309630333No Hit
TAGCTTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG10770.1693670515822531No Hit
TAGCTTTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT10610.16685092082522798No Hit
TAGCTTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC10580.1663791463082858No Hit
TAGCTTTGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCCCC10500.1651210809297732No Hit
TAGCTTTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA10100.15883075403721042No Hit
TAGCTTTGTCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAG10010.15741543048638382No Hit
TAGCTTTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG9980.15694365596944157No Hit
TAGCTTTGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGA9800.15411300886778834No Hit
TAGCTTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA9680.1522259108000195No Hit
TAGCTTTCTTTCGGCGTGGGTGTTTTTCACACCCATTGTCGTTACTCATG9430.14829445649216774No Hit
TAGCTTTCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGA9370.14735090745828333No Hit
TAGCTTTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA9280.1459355839074567No Hit
TAGCTTTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT9150.1438912276673738No Hit
TAGCTTTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC9090.1429476786334894No Hit
TAGCTTTCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTG9020.1418468714272909No Hit
TAGCTTTCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGCG8780.1380726752917532No Hit
TAGCTTTACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACA8760.1377581589471251No Hit
TAGCTTTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCACGG8400.13209686474381857No Hit
TAGCTTTGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCT8240.12958073398679346No Hit
TAGCTTTCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACCT7850.12344766526654474No Hit
TAGCTTTCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTT7720.12140330902646182No Hit
TAGCTTTCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTC7510.11810088740786637No Hit
TAGCTTTCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCA7450.11715733837398194No Hit
TAGCTTTCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTA7360.11574201482315533No Hit
TAGCTTTCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTC7350.11558475665084124No Hit
TAGCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT7300.1147984657892709No Hit
TAGCTTTCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGC7160.11259685137687393No Hit
TAGCTTTCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCCC7150.11243959320455985No Hit
TAGCTTTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT7120.11196781868761764No Hit
TAGCTTTGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAG7010.11023797879216289No Hit
TAGCTTTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA6960.10945168793059254No Hit
TAGCTTTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT6880.10819362255207997No Hit
TAGCTTTCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCATCG6780.10662104082893926No Hit
TAGCTTTGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTT6740.105992008139683No Hit
TAGCTTTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC6740.105992008139683No Hit
TAGCTTTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC6650.10457668458885637No Hit
TAGCTTTCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTCA6600.10379039372728602No Hit
TAGCTTTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC6580.10347587738265787No Hit
TAGCTTTCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTA6540.1028468446934016No Hit
TAGCTTTCCCGCCGTGTGTCTCCCATGCTCGGCACTTGTAGGTATTCGGA6510.1023750701764594No Hit
TAGCTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT6480.10190329565951718No Hit
TAGCTTTCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTCG6450.10143152114257498No Hit
TAGCTTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC6420.10095974662563277No Hit
TAGCTTTGACCCATTATACAAAAGGTACGCAGTCACCCAACAAAGTGGGC6410.10080248845331868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTTG193950.0339.0412
GCTTTGT67200.0330.6253
GCTTTCC138250.0329.902343
TAGCTTT666300.0323.796721
AGCTTTA62050.0322.759862
TTTGTCC31950.0319.624425
CTTTGTC46000.0318.750034
GCTTTGC88000.0318.536933
CTTTGCC60400.0308.44374
AGCTTTC382150.0307.03782
GCTTTAG13500.0304.11113
GCTTTGG39600.0303.617433
TCCCGTT28300.0303.551247
TTCCCGT29500.0303.483066
TTTACGG12000.0287.55
GCTTTAT24250.0287.381443
TTTGCCC31300.0284.376985
CTTTGTT16100.0281.78574
TTGTCCC13550.0278.800726
TTCCCCG21800.0277.74086
Produced by FastQC (version 0.11.2)