FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 kri319.3410000000eb9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 kri319.3410000000eb9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528543
Sequences flagged as poor quality0
Sequence length351
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT769515.0342711981278905No Hit
TCTGATTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA638344.176133743048118No Hit
TCTGATTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC426912.7929211019905886No Hit
TCTGATTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG407442.66554490125564No Hit
TCTGATTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT375362.455671839130466No Hit
TCTGATTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA362522.37167027685842No Hit
TCTGATTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT349472.2862948572594948No Hit
TCTGATTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA286861.876689108517065No Hit
TCTGATTCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC223521.462307570019293No Hit
TCTGATTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC208501.3640440602586907No Hit
TCTGATTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC206971.3540345283057134No Hit
TCTGATTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG176021.151554127034699No Hit
TCTGATTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA175191.1461241195046525No Hit
TCTGATTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT171201.1200208302939467No Hit
TCTGATTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC146890.960980489263305No Hit
TCTGATTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGT143300.9374940711514167No Hit
TCTGATTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG135240.8847641185102414No Hit
TCTGATTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACT130660.8548009444287796No Hit
TCTGATTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC124430.8140431770646949No Hit
TCTGATTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC122180.7993232771338458No Hit
TCTGATTGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTC116200.7602010542065222No Hit
TCTGATTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA110310.7216676272764325No Hit
TCTGATTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT108520.7099571291092236No Hit
TCTGATTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCA87420.5719171786465935No Hit
TCTGATTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC80520.5267761521919894No Hit
TCTGATTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC74440.48699971148996135No Hit
TCTGATTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA73070.478036927976511No Hit
TCTGATTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT72800.476270539984809No Hit
TCTGATTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC70700.4625319667160165No Hit
TCTGATTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC69150.4523915912080982No Hit
TCTGATTGTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTAC61990.40554959853926253No Hit
TCTGATTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCG61380.4015588701135657No Hit
TCTGATTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA60540.39606344080604866No Hit
TCTGATTACCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT57090.3734929275787465No Hit
TCTGATTCCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT54340.3555019387743753No Hit
TCTGATTCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCC53470.349810244134447No Hit
TCTGATTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGT53340.34895976102733123No Hit
TCTGATTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA52890.34601578104116143No Hit
TCTGATTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACT52520.3435951752747551No Hit
TCTGATTGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGC49500.323837798478682No Hit
TCTGATTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT48480.31716477717669705No Hit
TCTGATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTT44500.29112690974346156No Hit
TCTGATTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT43740.28615485465570806No Hit
TCTGATTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT43130.2821641262300112No Hit
TCTGATTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCAC42120.2755565267054967No Hit
TCTGATTGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC41980.27464062182091054No Hit
TCTGATTTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTC41580.2720237507220929No Hit
TCTGATTGAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAG40750.26659374319204626No Hit
TCTGATTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC40180.2628647018762312No Hit
TCTGATTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC39510.2584814427857116No Hit
TCTGATTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCG38190.24984576815961346No Hit
TCTGATTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA37830.24749058417067757No Hit
TCTGATTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT37090.24264937263786496No Hit
TCTGATTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT36960.2417988895307492No Hit
TCTGATTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT36920.24153720242086743No Hit
TCTGATTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA36670.23990165798410645No Hit
TCTGATTACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTA36460.23852780065722717No Hit
TCTGATTCGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAA36360.23787358288252275No Hit
TCTGATTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCT36250.23715394333034792No Hit
TCTGATTCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC36150.23649972555564353No Hit
TCTGATTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC36040.23578008600346864No Hit
TCTGATTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT35940.23512586822876425No Hit
TCTGATTGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC35560.2326398406848875No Hit
TCTGATTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC35190.2302192349184812No Hit
TCTGATTCCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCC34420.22518175805325727No Hit
TCTGATTCACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGC34370.22485464916590503No Hit
TCTGATTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA33690.22040596829791506No Hit
TCTGATTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCA33110.2166115052046295No Hit
TCTGATTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT32980.21576102209751377No Hit
TCTGATTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA32150.21033101456746717No Hit
TCTGATTCGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT31670.20719076924888602No Hit
TCTGATTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT31630.20692908213900424No Hit
TCTGATTCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC30520.19966726483978534No Hit
TCTGATTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG30460.1992747341749627No Hit
TCTGATTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT30290.19816256395796522No Hit
TCTGATTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTCCGAGCGT30090.1968541284085564No Hit
TCTGATTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGC29720.19443352264215005No Hit
TCTGATTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC28140.18409688180182043No Hit
TCTGATTCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGT27990.18311555513976382No Hit
TCTGATTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCG27990.18311555513976382No Hit
TCTGATTCCGTCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAAT27540.18017157515359397No Hit
TCTGATTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG27510.17997530982118265No Hit
TCTGATTGCCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAAGACCTT26740.17493783295595872No Hit
TCTGATTCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTC25420.16630215832986053No Hit
TCTGATTATCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTC24990.16348902189863157No Hit
TCTGATTGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTAC24990.16348902189863157No Hit
TCTGATTGGCGTCGGGACCATCCTCATCCTGCAAGCGTCGCCAATTAAGG24980.16342360012116114No Hit
TCTGATTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT24860.16263853879151585No Hit
TCTGATTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG24620.16106841613222528No Hit
TCTGATTCACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCGTATG24210.1583861232559372No Hit
TCTGATTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCT24180.15818985792352586No Hit
TCTGATTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCC24110.15773190548123278No Hit
TCTGATTCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCC23730.15524587793735603No Hit
TCTGATTGCCGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGA23090.15105888417924782No Hit
TCTGATTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA21930.14346995799267667No Hit
TCTGATTCCTTCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCA21920.14340453621520624No Hit
TCTGATTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT21790.1425540531080905No Hit
TCTGATTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCAC21290.13928296423456848No Hit
TCTGATTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC21270.1391521206796276TruSeq Adapter, Index 6 (97% over 36bp)
TCTGATTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG21090.13797452868515966No Hit
TCTGATTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGG20630.13496512692151938TruSeq Adapter, Index 19 (97% over 36bp)
TCTGATTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA20510.1341800655918741No Hit
TCTGATTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC20420.13359126959464013No Hit
TCTGATTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC20150.13182488160293823No Hit
TCTGATTACGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCC20030.13103982027329295No Hit
TCTGATTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTC19970.1306472896084703No Hit
TCTGATTGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACT19920.1303201807211181No Hit
TCTGATTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT19460.1273107789574778No Hit
TCTGATTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGG18930.12384342475154445No Hit
TCTGATTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGG18620.12181534964996077TruSeq Adapter, Index 7 (97% over 36bp)
TCTGATTCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG18530.1212265536527268No Hit
TCTGATTGCCTCTGACTGCCAAGGCATCCACCGTATGCGCTAATCAGAAG18500.12103028832031548No Hit
TCTGATTGACCCATTATACAAAAGGTACGCAGTCACCCAACAAAGTGGGC18360.1201143834357293No Hit
TCTGATTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCCTTTTCACGACG18330.11991811810331798No Hit
TCTGATTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG18190.11900221321873182No Hit
TCTGATTAGGCGTCGGGACCATCCTCATCCTGCAAGCGTCGCCAATTAAG18030.11795546477920477No Hit
TCTGATTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGC17740.116058233232562No Hit
TCTGATTGTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCG17490.11442268879580098No Hit
TCTGATTACCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA17420.11396473635350789No Hit
TCTGATTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACC17390.11376847102109656No Hit
TCTGATTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC17390.11376847102109656TruSeq Adapter, Index 6 (100% over 36bp)
TCTGATTGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT17160.11226377013927644No Hit
TCTGATTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT17000.11121702169974937No Hit
TCTGATTCGCCTCTGACTGCCAAGGCATCCACCGTATGCGCTAATCAGAA16940.11082449103492673No Hit
TCTGATTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGG16810.109974007927811No Hit
TCTGATTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA16640.10886183771081351No Hit
TCTGATTTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTC16570.10840388526852042No Hit
TCTGATTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT16320.1067683408317594No Hit
TCTGATTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA16300.10663749727681851No Hit
TCTGATTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTA16190.10591785772464367No Hit
TCTGATTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA16030.10487110928511661No Hit
TCTGATTGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC15990.10460942217523485No Hit
TCTGATTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGC15880.10388978262306TruSeq Adapter, Index 11 (97% over 36bp)
TCTGATTCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT15830.1035626737357078No Hit
TCTGATTACCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC15760.1031047212934147No Hit
TCTGATTGCGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCC15730.10290845596100338No Hit
TCTGATTGCGTCGGGACCATCCTCATCCTGCAAGCGTCGCCAATTAAGGC15660.1024505035187103No Hit
TCTGATTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGG15350.10042242841712663TruSeq Adapter, Index 10 (97% over 36bp)
TCTGATTATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC15290.10002989775230399TruSeq Adapter, Index 11 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGCC273400.0340.961944
TGATTCC372850.0340.373473
TCTGATT1529050.0339.799221
CTGATTG522750.0338.89532
TGATTGC352400.0338.83233
GATTCCC251900.0338.220524
ATTGCCC162500.0337.993845
ATTGGCC22900.0337.467255
CTGATTC643850.0335.729982
GATTCGG26800.0335.345154
TGATTGG48550.0333.630253
CTGATTA226800.0332.754642
TGATTGT104250.0330.438843
GATTGGC31100.0329.469454
TTGCCCC129250.0328.450686
CTGATTT156250.0328.10882
GATTGTC90400.0326.872254
ATTGCCT61350.0326.442545
GATTGCG47750.0324.408364
ATTCCCG57850.0324.127045
Produced by FastQC (version 0.11.2)