##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AGWYV l01n01 ff.3410000000ebb6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609357 Sequences flagged as poor quality 0 Sequence length 351 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.125788330978395 33.0 32.0 34.0 32.0 34.0 2 32.235200383354915 33.0 32.0 34.0 32.0 34.0 3 32.705725871697545 33.0 32.0 34.0 32.0 34.0 4 32.78827518187204 33.0 32.0 34.0 32.0 34.0 5 32.45362734817193 33.0 32.0 34.0 32.0 34.0 6 32.55815556397974 33.0 32.0 34.0 32.0 34.0 7 36.67571719041547 37.0 37.0 37.0 37.0 37.0 8 34.619231419348594 37.0 33.0 37.0 32.0 37.0 9 33.82594275605269 34.0 33.0 37.0 32.0 37.0 10-19 36.200488875979104 37.5 36.3 37.7 33.8 37.7 20-29 37.02184794791888 38.2 37.9 38.7 34.8 38.7 30-39 37.07451789345162 38.0 37.9 39.0 34.7 39.0 40-49 37.09755988689717 38.0 37.9 39.0 35.0 39.0 50-59 37.04218397425483 38.2 37.6 39.0 34.9 39.0 60-69 36.9004929786644 38.0 37.1 39.0 34.3 39.0 70-79 36.85656142458362 38.0 37.0 39.0 34.0 39.0 80-89 36.922804037698754 38.0 37.4 39.0 34.3 39.0 90-99 36.86446746324405 38.4 37.5 39.0 33.9 39.0 100-109 36.776778965368415 38.0 37.2 39.0 33.9 39.0 110-119 36.6424796958105 38.0 37.1 39.0 33.3 39.0 120-129 36.506302709249255 38.3 37.0 39.0 32.5 39.0 130-139 36.107218920928126 38.0 37.0 39.0 30.7 39.0 140-149 35.16411200659055 38.0 36.0 39.0 26.1 39.0 150-159 34.916643445467926 38.0 35.5 39.0 22.5 39.0 160-169 34.2063557159432 38.0 34.3 39.0 15.2 39.0 170-179 32.85264680638772 37.5 31.4 38.1 14.9 39.0 180-189 32.08746235786247 37.0 28.6 38.0 14.4 38.9 190-199 30.909492957330436 37.0 24.6 38.0 14.0 38.0 200-209 29.364806180941553 36.0 17.8 37.0 13.4 38.0 210-219 28.41614258964778 34.1 15.0 37.0 13.3 37.6 220-229 27.665835954949234 32.9 14.2 37.0 13.1 37.0 230-239 26.648525248745806 30.8 14.0 37.0 13.0 37.0 240-249 25.728817261473978 26.2 14.0 37.0 13.0 37.0 250-259 24.84498413901867 24.9 14.0 37.0 13.0 37.0 260-269 23.991951844321143 24.0 14.0 36.9 13.0 37.0 270-279 23.097233477255532 19.7 14.0 34.6 13.0 37.0 280-289 22.559196169076582 15.0 14.0 33.0 13.0 37.0 290-299 22.008485173715897 15.0 14.0 32.8 13.0 37.0 300-309 21.192923688412538 15.0 14.0 29.7 13.0 37.0 310-319 20.32596868502372 15.0 14.0 26.0 13.0 36.2 320-329 19.825968685023724 14.2 14.0 25.7 13.0 34.3 330-339 18.983612233879317 14.0 14.0 25.0 13.0 32.5 340-349 18.581411061167753 14.0 14.0 24.6 13.0 27.7 350-351 18.2449278501765 14.0 14.0 24.0 13.0 26.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.021942563993000874 1101 2 0.4376157549770596 1101 3 0.3333996462444091 1101 4 0.22387791119835043 1101 5 0.3750220695522728 1101 6 0.2389145076885697 1101 7 0.08985323207985374 1101 8 0.1823580060225254 1101 9 0.17433738583913794 1101 10-19 0.1449614353120694 1101 20-29 0.16657621988658633 1101 30-39 0.13772606368279128 1101 40-49 0.21596153296177079 1101 50-59 0.1716259572519121 1101 60-69 0.29748800121353725 1101 70-79 0.2897670528364671 1101 80-89 0.29318782631273876 1101 90-99 0.4180088998169964 1101 100-109 0.39097572385900037 1101 110-119 0.3585499925977018 1101 120-129 0.2764556434175063 1101 130-139 0.3074646607192335 1101 140-149 0.6970584876573369 1101 150-159 0.5388211038271393 1101 160-169 0.49835348187604467 1101 170-179 0.6696486983654495 1101 180-189 0.4045802742218214 1101 190-199 0.5116364865845071 1101 200-209 0.46811124051055586 1101 210-219 0.17731612365731309 1101 220-229 0.18680775337907818 1101 230-239 0.31221627595862955 1101 240-249 0.45761674236158 1101 250-259 0.19769048988733573 1101 260-269 0.41071711392450894 1101 270-279 0.3725096771080345 1101 280-289 0.3983428407719174 1101 290-299 0.3894707706723146 1101 300-309 0.30877018233410425 1101 310-319 0.4554127490182047 1101 320-329 0.3445389293423453 1101 330-339 0.19094771774264885 1101 340-349 0.14780077406328473 1101 350-351 0.03782391870477042 1102 1 0.031890452071202446 1102 2 0.3451469693906404 1102 3 -0.28890677664738007 1102 4 0.11534846898536699 1102 5 -0.07314401717447083 1102 6 0.1580557869158099 1102 7 0.06348601642019958 1102 8 0.10368652776897136 1102 9 0.06030485657466045 1102 10-19 0.19874616375506804 1102 20-29 0.20737016008557418 1102 30-39 0.30417035045123697 1102 40-49 0.2942324343165126 1102 50-59 0.21773361572063976 1102 60-69 0.22038250256998992 1102 70-79 0.29734423857811976 1102 80-89 0.19735493220846934 1102 90-99 0.21293758871473045 1102 100-109 0.14349686712456844 1102 110-119 -0.6151556604338779 1102 120-129 0.07887751697720091 1102 130-139 0.02675905164994674 1102 140-149 0.7975164519003854 1102 150-159 0.3446832872217698 1102 160-169 0.07411229241696304 1102 170-179 0.48448311413250167 1102 180-189 0.3367704617422973 1102 190-199 0.30914332792174193 1102 200-209 0.4563034871683591 1102 210-219 -0.07344206053904756 1102 220-229 0.09386171686389844 1102 230-239 0.4283738387266318 1102 240-249 0.3999342125534824 1102 250-259 0.24256644766272828 1102 260-269 0.22983426115897032 1102 270-279 0.1282617656717413 1102 280-289 0.3769060677747902 1102 290-299 0.242507076440031 1102 300-309 0.506430253553031 1102 310-319 0.6376384136407083 1102 320-329 0.02834815849417538 1102 330-339 0.3422993220592758 1102 340-349 0.36469383080915563 1102 350-351 0.06517993154175983 1103 1 0.25422920125831894 1103 2 0.08629095846583823 1103 3 -0.1605515950842289 1103 4 0.11682237762239112 1103 5 0.2229217344521075 1103 6 0.304568665641888 1103 7 0.12278618173947109 1103 8 0.06575158530348801 1103 9 0.05315538573920264 1103 10-19 0.21211527094343552 1103 20-29 -0.0520227812839309 1103 30-39 0.034725356786090344 1103 40-49 -0.23049136912361945 1103 50-59 -0.7716614318910402 1103 60-69 0.04657747238435661 1103 70-79 -0.11579597032238809 1103 80-89 -0.6199157696863651 1103 90-99 0.02172375259326742 1103 100-109 -0.05789848374910633 1103 110-119 -0.17955620409323103 1103 120-129 0.045723698157360104 1103 130-139 0.048272587136438005 1103 140-149 0.6884035900426824 1103 150-159 0.011810031842436786 1103 160-169 -0.10005153635337649 1103 170-179 0.2087709669223088 1103 180-189 -0.1579982687190764 1103 190-199 -0.24963659097180724 1103 200-209 -0.015990832752773798 1103 210-219 0.0964260481430479 1103 220-229 0.052372709650597926 1103 230-239 -0.08064120039555078 1103 240-249 -0.11912156414981112 1103 250-259 -0.20593765950951237 1103 260-269 -0.31345150428491664 1103 270-279 -0.19368898789747035 1103 280-289 -0.31321971221462164 1103 290-299 0.06126272515570719 1103 300-309 0.06388273290425062 1103 310-319 0.15887797633505585 1103 320-329 0.021708541138920623 1103 330-339 0.07031649092873593 1103 340-349 0.03039764061871253 1103 350-351 0.10638266034672128 2112 1 -0.2202617979079733 2112 2 0.2832038653529736 2112 3 0.30021592288154864 2112 4 0.27953306291305324 2112 5 0.23684871344845249 2112 6 0.1832662324234775 2112 7 0.137097676919808 2112 8 0.04513481345686188 2112 9 0.3017389858676154 2112 10-19 0.019138587216083636 2112 20-29 0.11439755839964505 2112 30-39 0.005103215910402525 2112 40-49 0.01874511976643589 2112 50-59 -0.11465637520743144 2112 60-69 -0.09640283959827656 2112 70-79 -0.7954823155414061 2112 80-89 -0.8099184277777312 2112 90-99 -0.27030938757491896 2112 100-109 -0.18582070815354257 2112 110-119 -0.029699125801002424 2112 120-129 -0.4006481254431833 2112 130-139 -0.3408176830660423 2112 140-149 0.355617847192903 2112 150-159 -1.0301886793381883 2112 160-169 -0.4200691333914577 2112 170-179 0.1383748482089402 2112 180-189 -0.33662721415090857 2112 190-199 -0.29164847429658636 2112 200-209 -0.07596672063022325 2112 210-219 -0.15679596085344372 2112 220-229 -0.2727237743334179 2112 230-239 -0.12914407184409882 2112 240-249 -0.18005337897944074 2112 250-259 -0.267724204519201 2112 260-269 -1.0558248328226512 2112 270-279 -0.06419770083700627 2112 280-289 -0.07911324500734196 2112 290-299 -0.03744644415525045 2112 300-309 -0.0016389825979423733 2112 310-319 -0.27177932154549467 2112 320-329 0.08688596676560678 2112 330-339 0.18906818181400808 2112 340-349 0.042561445301846135 2112 350-351 0.20580789738615834 1113 1 -0.3033385296215485 1113 2 -0.630127641885462 1113 3 -0.20996455616401022 1113 4 0.011438072455938197 1113 5 0.13751135653114943 1113 6 0.04622464730253739 1113 7 0.08432551930928867 1113 8 0.15815310572290286 1113 9 0.32109581230876216 1113 10-19 -0.03177913574061364 1113 20-29 0.17602190950741203 1113 30-39 0.186799922720283 1113 40-49 0.15938936075272636 1113 50-59 0.13444248665907566 1113 60-69 0.29135923699802646 1113 70-79 0.3868119080440664 1113 80-89 -0.07596261221684131 1113 90-99 0.08377597045215879 1113 100-109 0.1853996393073274 1113 110-119 0.15662490106464588 1113 120-129 0.24066402960021804 1113 130-139 0.36530187410153303 1113 140-149 0.5892963742136033 1113 150-159 -1.384080025809702 1113 160-169 0.1530288939408564 1113 170-179 0.5243858806450703 1113 180-189 0.23792602114671269 1113 190-199 0.3096506554853917 1113 200-209 0.6198318055259406 1113 210-219 0.41584944636351295 1113 220-229 0.1949704456351533 1113 230-239 0.07406321683282968 1113 240-249 0.27555509216010066 1113 250-259 0.2188190640054053 1113 260-269 -0.43814706535157555 1113 270-279 0.2776046548254101 1113 280-289 0.20701293285694078 1113 290-299 0.22902294692149638 1113 300-309 0.3430059059022028 1113 310-319 0.18752002260414358 1113 320-329 0.3865008768745426 1113 330-339 0.36320851676568466 1113 340-349 0.27621983675924966 1113 350-351 0.4285335822048246 2113 1 0.16485356374928273 2113 2 0.312855957466482 2113 3 0.2516965652539369 2113 4 0.11907300554519651 2113 5 0.2210342904843614 2113 6 -0.15802716187513255 2113 7 -0.03855968104295471 2113 8 0.02673593302868227 2113 9 0.16355675169234019 2113 10-19 -0.038739661702628325 2113 20-29 0.11531180213334835 2113 30-39 -0.0012058742922818055 2113 40-49 0.03971711595027472 2113 50-59 0.08423973121153949 2113 60-69 0.10914294185524653 2113 70-79 0.11816417321873018 2113 80-89 0.189643935331965 2113 90-99 0.07413723712873832 2113 100-109 -6.839973981129788E-4 2113 110-119 0.09461068807331685 2113 120-129 0.19598470185634653 2113 130-139 0.27830237696260696 2113 140-149 0.5201745767308381 2113 150-159 -0.07189161134640898 2113 160-169 0.10379686255770793 2113 170-179 0.37333468325577 2113 180-189 0.2453348778566422 2113 190-199 0.3093993582793928 2113 200-209 0.18747326269863862 2113 210-219 0.2557538160994923 2113 220-229 0.1810528379650016 2113 230-239 -0.07570698477156412 2113 240-249 0.11887013983857031 2113 250-259 0.3512847342383232 2113 260-269 0.15680039200023188 2113 270-279 0.2893892027470919 2113 280-289 0.3204895204857614 2113 290-299 0.30537558874198467 2113 300-309 0.20444688394585953 2113 310-319 0.2759067540938922 2113 320-329 0.31683990922586247 2113 330-339 0.17706877205378646 2113 340-349 0.13397692975566144 2113 350-351 0.11513692348660953 1112 1 -0.3150177100937306 1112 2 -0.0031297275568462624 1112 3 0.02194467869693284 1112 4 0.07869601726864062 1112 5 0.08993327308067478 1112 6 0.09178991638444245 1112 7 0.04163130689699557 1112 8 0.16939804054484142 1112 9 0.2607289827944044 1112 10-19 -0.010369376955274845 1112 20-29 -0.05193453135374426 1112 30-39 -0.010528430490538199 1112 40-49 0.019052440492401956 1112 50-59 0.06890552709606368 1112 60-69 -0.07258320105623994 1112 70-79 -0.01891752772846189 1112 80-89 0.01599388389978884 1112 90-99 0.06982044264317011 1112 100-109 -0.03578617884021895 1112 110-119 -0.034381044914667314 1112 120-129 -0.037428533569830336 1112 130-139 -0.11405931467544406 1112 140-149 0.40541265847003416 1112 150-159 0.2900240723864087 1112 160-169 0.05840812585855559 1112 170-179 0.14846289697417347 1112 180-189 -0.041029062167631025 1112 190-199 0.08612554777620218 1112 200-209 -0.3892955077679261 1112 210-219 -0.4442848199852527 1112 220-229 -0.24683662675375473 1112 230-239 -0.4777438934328089 1112 240-249 -0.22723501969435844 1112 250-259 -0.10803850587215891 1112 260-269 0.16845108452578472 1112 270-279 0.16235260630664428 1112 280-289 0.27222430844422973 1112 290-299 0.23322191743178067 1112 300-309 0.18637873576012254 1112 310-319 0.24245470417107384 1112 320-329 0.2998000676714767 1112 330-339 0.33579411429237993 1112 340-349 0.24916103000569834 1112 350-351 0.25897625208700603 2114 1 0.10838746699727864 2114 2 0.1646051193547038 2114 3 0.10445093435873076 2114 4 0.21652764252960566 2114 5 0.26803542904366395 2114 6 0.20133806598061454 2114 7 0.03958991081454144 2114 8 0.21205277393802646 2114 9 0.2600055734719362 2114 10-19 0.12842838584043648 2114 20-29 0.10142210822299802 2114 30-39 0.10324739633989566 2114 40-49 0.05437848879582674 2114 50-59 -0.0016007496990440018 2114 60-69 0.10348769874710229 2114 70-79 0.16587884677159792 2114 80-89 0.10820678895312597 2114 90-99 0.15818149786165492 2114 100-109 0.08594767887004906 2114 110-119 0.2268679700497458 2114 120-129 0.06735177961763839 2114 130-139 -0.0847027953959838 2114 140-149 0.5285531815437494 2114 150-159 0.1985374621646372 2114 160-169 0.4159830139638032 2114 170-179 0.4285908428859173 2114 180-189 0.09501677841165446 2114 190-199 0.080177721031923 2114 200-209 -0.4791462578580514 2114 210-219 0.14610125300349353 2114 220-229 0.22319931205882426 2114 230-239 0.06817912606144105 2114 240-249 -0.36058618086792293 2114 250-259 -0.0393800725247786 2114 260-269 0.04079368305271558 2114 270-279 0.290508184828866 2114 280-289 0.3406324949993049 2114 290-299 0.3449275743707645 2114 300-309 0.39751304157775635 2114 310-319 0.275199359781503 2114 320-329 0.15666670553723705 2114 330-339 0.30862846859469073 2114 340-349 0.2205529345676851 2114 350-351 0.10193719489256026 1114 1 0.30545630788319045 1114 2 0.25326931850023016 1114 3 0.2825243478988071 1114 4 0.046370426261034936 1114 5 -0.009059372505774377 1114 6 0.1918957361505491 1114 7 0.07548520499300082 1114 8 0.14507975940776419 1114 9 -0.003946437043609308 1114 10-19 0.014548128170176255 1114 20-29 0.1667290536850956 1114 30-39 0.21843984083501766 1114 40-49 0.22732787124546405 1114 50-59 0.06690139221979763 1114 60-69 0.11573483047941124 1114 70-79 0.24839182915462032 1114 80-89 0.2999109046700781 1114 90-99 0.39460457954995576 1114 100-109 0.20552488271622593 1114 110-119 0.23924252564569315 1114 120-129 0.31051823391119626 1114 130-139 0.04416523270126049 1114 140-149 0.5039732167104418 1114 150-159 0.17176093309878837 1114 160-169 0.35418785541421727 1114 170-179 0.401285459658439 1114 180-189 0.20813216469959883 1114 190-199 0.2676962951318096 1114 200-209 0.0831233145868886 1114 210-219 0.34016426915014364 1114 220-229 0.43996268848761844 1114 230-239 0.049018695888985775 1114 240-249 0.01965212555159468 1114 250-259 0.015583058245908887 1114 260-269 0.25936396761079195 1114 270-279 0.1674972647760562 1114 280-289 0.14813807235740128 1114 290-299 -0.035683403773543176 1114 300-309 0.23117017210393698 1114 310-319 0.34525946971227484 1114 320-329 0.2338553724440331 1114 330-339 0.2903650105260027 1114 340-349 0.2195803122370812 1114 350-351 -0.24418249000209258 1109 1 0.3391163787866205 1109 2 0.011694808159916192 1109 3 0.13391996386093297 1109 4 0.09291365579351663 1109 5 0.3477121670213634 1109 6 0.11898248981930237 1109 7 0.021164554577715933 1109 8 0.1546700655932156 1109 9 0.1380287662111641 1109 10-19 0.04065881819816042 1109 20-29 0.14596931339699637 1109 30-39 0.20661568753307336 1109 40-49 0.23427053507514728 1109 50-59 0.13743067055565206 1109 60-69 0.04771162983557531 1109 70-79 0.22240888679333892 1109 80-89 0.1644084682332263 1109 90-99 0.16987950007244734 1109 100-109 0.02181383185816088 1109 110-119 0.14365584523085317 1109 120-129 0.13834802835953752 1109 130-139 0.19202175192177862 1109 140-149 0.5629769357854855 1109 150-159 -0.06445033910915754 1109 160-169 0.1446529760330293 1109 170-179 0.39246227473739737 1109 180-189 -0.07705464467388623 1109 190-199 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120-129 -0.0038466790343889556 2105 130-139 -0.23850923973213156 2105 140-149 -2.8548647862034144 2105 150-159 0.08948734562923022 2105 160-169 0.06829549886554531 2105 170-179 0.360249458551408 2105 180-189 -0.05324840549038612 2105 190-199 0.2322246870291771 2105 200-209 0.3503722139920704 2105 210-219 0.30528499174871726 2105 220-229 -0.025391741692239123 2105 230-239 0.11807500595212161 2105 240-249 0.38027738475341266 2105 250-259 0.27224131409920105 2105 260-269 0.08734808181169029 2105 270-279 -0.19567359524623384 2105 280-289 0.1318710024371157 2105 290-299 -0.05280206854860481 2105 300-309 -0.0800890563119836 2105 310-319 0.015654777941158216 2105 320-329 -0.17727291489993746 2105 330-339 -0.2021372729433395 2105 340-349 -0.1319119006961209 2105 350-351 0.04703319666352357 2104 1 -0.06304572337842984 2104 2 -0.3963524681909725 2104 3 -0.14908343683509884 2104 4 -0.18127754744998725 2104 5 -0.35538634503589606 2104 6 -0.1751834508767871 2104 7 -0.09693969766439636 2104 8 -0.1871951115333701 2104 9 -0.14548496415537215 2104 10-19 -0.09203723788731821 2104 20-29 -0.10621287839111915 2104 30-39 -0.07294812804541806 2104 40-49 0.12939272954187686 2104 50-59 0.15650923879832135 2104 60-69 -0.08764715117278854 2104 70-79 0.012573470159942701 2104 80-89 0.05210747530206561 2104 90-99 -0.0639581012306536 2104 100-109 0.09582263115402156 2104 110-119 0.07813719505692518 2104 120-129 -0.08281128009869576 2104 130-139 0.07256954945605543 2104 140-149 -2.540808350582779 2104 150-159 0.35382824846542604 2104 160-169 -0.0411937718850055 2104 170-179 0.31408796553576934 2104 180-189 0.022140807903753057 2104 190-199 0.290705462343805 2104 200-209 0.19450874282330943 2104 210-219 0.3729827877707983 2104 220-229 -0.05054934321694304 2104 230-239 0.09648160373965808 2104 240-249 0.18659546333941535 2104 250-259 -0.04195208245479165 2104 260-269 0.018049836197654656 2104 270-279 -0.2586758817865089 2104 280-289 -0.1442576713952448 2104 290-299 -0.1210376773624624 2104 300-309 -0.11763229867239033 2104 310-319 -0.0016566982276380315 2104 320-329 -0.020155961837026837 2104 330-339 0.05459040242452318 2104 340-349 -0.14308868446760314 2104 350-351 -0.020076374655186413 2111 1 -0.032551537120561136 2111 2 -0.2430291598045322 2111 3 -0.15978904511714376 2111 4 -0.10633123266579503 2111 5 0.08018925756221051 2111 6 -0.11868299560563145 2111 7 -0.06514235384763367 2111 8 -4.937339157748966E-4 2111 9 -0.18170115401692044 2111 10-19 -0.07657976628670582 2111 20-29 0.051684055403597995 2111 30-39 -0.20228206783672675 2111 40-49 -0.2148942482488465 2111 50-59 0.1951425143513532 2111 60-69 -0.24578686061185095 2111 70-79 -0.04248314042433776 2111 80-89 -0.06105244868967219 2111 90-99 0.24655851285208996 2111 100-109 -0.007337386928519152 2111 110-119 -0.08925386383664602 2111 120-129 -0.16776312485572475 2111 130-139 -0.08437591252334187 2111 140-149 -2.641890266459619 2111 150-159 0.2527050823271537 2111 160-169 0.06653745518303822 2111 170-179 0.2155104473345375 2111 180-189 0.10770884046942797 2111 190-199 0.6326876860258608 2111 200-209 -1.095852659933641 2111 210-219 -0.2179111261369755 2111 220-229 0.3987272268484112 2111 230-239 0.08090553496441899 2111 240-249 0.04739139878799392 2111 250-259 0.09112519560105525 2111 260-269 -0.06718738167429095 2111 270-279 -0.14536902645923533 2111 280-289 -0.14011173143458322 2111 290-299 -0.17529180035709757 2111 300-309 -0.3017019566566148 2111 310-319 -0.25994231255715405 2111 320-329 -0.1405828175748738 2111 330-339 -0.21525170038506758 2111 340-349 -0.18451538752174557 2111 350-351 0.045450595830669016 2110 1 0.25864748044494235 2110 2 0.26888029162036275 2110 3 0.15457827114984468 2110 4 0.06546524930191566 2110 5 -0.0074440288739978655 2110 6 -0.16052761500422008 2110 7 -0.02291331410419417 2110 8 -0.06144113401549589 2110 9 -0.2874246312869033 2110 10-19 -0.08058816694243376 2110 20-29 -0.2624119231594122 2110 30-39 -0.22952861770455257 2110 40-49 -0.1757921709035486 2110 50-59 0.026878212631103793 2110 60-69 -0.330761098183217 2110 70-79 -0.07535435415173453 2110 80-89 -0.2659131832235815 2110 90-99 -0.35402737447476085 2110 100-109 -0.37796747046819235 2110 110-119 -0.3332777342785036 2110 120-129 -0.31282204000211067 2110 130-139 -0.29807481842100714 2110 140-149 -0.16676929632980375 2110 150-159 -0.23853680356386775 2110 160-169 -0.33796062665645366 2110 170-179 -3.256311177583168 2110 180-189 -0.4322993532218291 2110 190-199 -0.2770790160982983 2110 200-209 -1.9734913003629764 2110 210-219 -1.1545869896527954 2110 220-229 -0.17955253859454245 2110 230-239 -0.1831471205171411 2110 240-249 -0.13695725631682265 2110 250-259 -0.007727930158765162 2110 260-269 -0.10979132220515808 2110 270-279 -0.026005519025797952 2110 280-289 -0.15608162780480228 2110 290-299 -0.20331093001892597 2110 300-309 -0.3363893240826279 2110 310-319 -0.338413237181058 2110 320-329 -0.24788065986915342 2110 330-339 -0.12868143119582953 2110 340-349 -0.20169208485782875 2110 350-351 0.04771346133064114 2109 1 0.0022386817727522157 2109 2 0.22999729564577365 2109 3 0.19771160575502478 2109 4 0.0033371229307022077 2109 5 -0.2330933411200249 2109 6 -0.06319564255253596 2109 7 -0.029377629116495996 2109 8 2.470587463960783E-4 2109 9 -0.07628549703656518 2109 10-19 -0.13667168946376052 2109 20-29 -0.28947734086740695 2109 30-39 -0.2122540502932182 2109 40-49 -0.0820514188574677 2109 50-59 -0.05623667376071495 2109 60-69 0.020751540846099203 2109 70-79 -0.15277879660361293 2109 80-89 -0.1518016926766137 2109 90-99 -0.0428836997127533 2109 100-109 -0.26244893640482303 2109 110-119 -0.12645805745609096 2109 120-129 -0.27332397639678874 2109 130-139 -0.0032533482872736386 2109 140-149 0.2500195126443785 2109 150-159 -0.1874148583934101 2109 160-169 -0.23604706807812903 2109 170-179 -3.3057335596347954 2109 180-189 -0.17491655756392888 2109 190-199 0.28373711225700404 2109 200-209 -0.0635115228098897 2109 210-219 -0.22081722030315376 2109 220-229 -0.051805166558757776 2109 230-239 -0.17951799256247014 2109 240-249 -0.6010351127374491 2109 250-259 -0.43463820735890835 2109 260-269 0.06122374924112961 2109 270-279 -0.1881689583679922 2109 280-289 -0.17811900117603585 2109 290-299 0.026156380204856333 2109 300-309 -0.30562777725622325 2109 310-319 -0.509123655556774 2109 320-329 -0.1968674389675371 2109 330-339 -0.176986885533168 2109 340-349 -0.1689545760343556 2109 350-351 -0.5490451284398503 2108 1 0.17155635581431028 2108 2 -0.08094384360724405 2108 3 0.06307491318725766 2108 4 -0.0040344095840794125 2108 5 -0.0973664296780683 2108 6 0.03410451364494094 2108 7 -0.038906861771657475 2108 8 -0.08956273621969757 2108 9 -0.05079327725287186 2108 10-19 -0.08485703062672911 2108 20-29 -0.3298508703373244 2108 30-39 -0.1346092908175578 2108 40-49 -0.07872636992217963 2108 50-59 -0.045238136876356805 2108 60-69 -0.09117439386730553 2108 70-79 0.024400889815112237 2108 80-89 -0.016575048154400918 2108 90-99 -0.18515702553278857 2108 100-109 -0.09073323559219659 2108 110-119 0.13396017612834044 2108 120-129 0.07766619700217348 2108 130-139 0.11509457665885492 2108 140-149 0.4679122504501052 2108 150-159 0.3537900568842929 2108 160-169 -0.10259595393533516 2108 170-179 -0.09890806583326395 2108 180-189 0.03539734005751427 2108 190-199 -0.020929416006829626 2108 200-209 0.20093259210399594 2108 210-219 -0.225141517908785 2108 220-229 -0.05446356859921053 2108 230-239 0.023547274258412187 2108 240-249 0.2335515373208743 2108 250-259 0.131040579441418 2108 260-269 0.27828175979928815 2108 270-279 0.25421000835600793 2108 280-289 0.1506287102342867 2108 290-299 0.1968539700184131 2108 300-309 0.05708120113316539 2108 310-319 -0.1806073734522613 2108 320-329 -0.07667367500368272 2108 330-339 -0.050500795017715205 2108 340-349 -0.040731510213920075 2108 350-351 0.2298542724736059 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 1.0 15 0.0 16 2.0 17 8.0 18 74.0 19 476.0 20 2170.0 21 5742.0 22 8726.0 23 14563.0 24 24329.0 25 35104.0 26 47184.0 27 51380.0 28 54284.0 29 47344.0 30 45081.0 31 45160.0 32 43539.0 33 40592.0 34 46596.0 35 51340.0 36 39822.0 37 5839.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 99.70033986644938 0.10617749529421996 0.13883487019924282 0.05464776805714877 2 0.03183683784710769 99.80733789880152 0.037252382429347655 0.12357288092202108 3 0.1634509819366972 99.7699214089606 0.02642129326486772 0.040206315837842184 4 0.042175604776838536 0.05464776805714877 0.08385888731892797 99.81931773984708 5 99.6837650178795 0.055632412526646934 0.2512484471336179 0.009354122460232673 6 0.060227420050971765 0.06482242757529658 99.78124482035983 0.09370533201390974 7 0.733231914952975 0.3754777577019711 97.81704321112255 1.07424711622251 8 42.006081820673266 12.214350536713289 8.084095201991607 37.695472440621835 9 19.99747274586162 8.427407907023305 14.831043214404694 56.74407613271038 10-19 22.209689886224332 15.830719922803874 26.17752811570229 35.78206207526951 20-29 20.956779031011376 20.28157549679416 27.348024228818247 31.413621243376216 30-39 23.852536362099723 22.596770037925225 25.399412823681356 28.151280776293703 40-49 21.3938298895393 22.56060076441232 27.99299917782187 28.05257016822651 50-59 22.87993737661174 19.10638591170693 26.580723615220634 31.432953096460697 60-69 22.982028597357544 21.886463928370397 26.61516976091191 28.516337713360144 70-79 22.747699624358134 18.788838070293767 25.784966776454528 32.67849552889357 80-89 21.457044064481085 24.232198858797062 24.873743962898594 29.43701311382326 90-99 22.013548707900295 21.996990270071567 24.678324857185522 31.311136164842612 100-109 21.077775425571545 23.007350370964804 24.537504287306128 31.377369916157523 110-119 21.72947877845007 24.06054250628121 23.969495714334947 30.24048300093377 120-129 19.82217320880863 26.270626250293343 24.80263622145967 29.10456431943836 130-139 21.350554765104857 24.182129687523076 24.136885274149638 30.33043027322243 140-149 21.690638113722844 24.815571957911693 24.628243316847648 28.865546611517818 150-159 21.297170624116895 24.144762429905622 24.31438385051784 30.243683095459637 160-169 21.8786395952848 22.433880702754003 28.579540167675134 27.107939534286064 170-179 18.595782111307493 24.945984045477445 26.938018271719205 29.520215571495857 180-189 19.142735703372573 27.180486972333128 26.550839655571362 27.125937668722933 190-199 17.988289295109436 28.56507761460031 22.591813994095418 30.85481909619484 200-209 18.09916323258176 25.981079396983937 26.061623327806743 29.858134042627565 210-219 17.001073919253876 25.889281944502795 25.305256296475232 31.804387839768093 220-229 15.135760340122511 26.458205363720346 27.83400007450478 30.572034221652366 230-239 17.57186962189154 28.136860352282874 26.733758163729664 27.557511862095925 240-249 16.06398219762799 28.605661377484793 27.169819990580237 28.160536434306984 250-259 16.67738835440911 29.11781734445238 26.619691451707766 27.585102849430744 260-269 15.274373002606357 30.41188033410978 24.71076870259549 29.60297796068837 270-279 16.801910341870258 29.63232220723192 25.299738133760364 28.26602931713746 280-289 14.547399964224583 28.076693957729216 26.775650398698957 30.60025567934725 290-299 14.205339137923792 29.208109271940515 24.94133158110322 31.645220009032478 300-309 14.100043160249246 30.947720301891994 26.4098877997627 28.542348738096056 310-319 13.518645641002836 31.219766281468214 22.988974113528542 32.272613964000406 320-329 11.256603271973573 33.731326627904494 24.034679178215725 30.977390921906206 330-339 12.50851307197587 34.65075481203957 21.825235453108768 31.015496662875787 340-349 9.487265917111289 38.89476575956812 21.647776803344115 29.970191519976474 350-351 7.193238118213133 40.71644372674803 18.64153525765684 33.448782897381996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.25 4 0.0 5 0.0 6 0.5 7 0.0 8 0.0 9 0.25 10 0.75 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.5 18 0.75 19 0.5 20 0.0 21 0.5 22 0.25 23 0.0 24 1.0 25 1.0 26 1.25 27 1.5 28 1.0 29 2.5 30 3.25 31 7.5 32 7.75 33 16.25 34 34.75 35 66.25 36 123.25 37 231.0 38 398.25 39 705.75 40 1200.25 41 2007.0 42 3159.0 43 4804.25 44 6943.75 45 9542.25 46 12132.25 47 14178.75 48 15371.75 49 17649.5 50 24115.5 51 22632.5 52 22818.25 53 17308.5 54 15417.75 55 5410.5 56 2939.25 57 1710.5 58 1527.0 59 354.75 60 179.0 61 110.0 62 70.75 63 33.0 64 17.75 65 12.75 66 9.0 67 5.25 68 4.75 69 3.75 70 3.25 71 1.5 72 1.0 73 1.25 74 0.0 75 0.75 76 1.0 77 0.25 78 0.25 79 0.25 80 0.25 81 0.25 82 0.25 83 0.25 84 0.5 85 0.75 86 0.25 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.25 94 0.25 95 0.25 96 0.25 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-19 0.0 20-29 0.0 30-39 0.0 40-49 0.0 50-59 0.0 60-69 0.0 70-79 0.0 80-89 0.0 90-99 0.0 100-109 0.0 110-119 0.0 120-129 0.0 130-139 0.0 140-149 1.6410741158302934E-5 150-159 0.0 160-169 1.6410741158302934E-5 170-179 0.0 180-189 0.0 190-199 0.0 200-209 1.6410741158302934E-5 210-219 3.282148231660587E-5 220-229 1.6410741158302934E-5 230-239 1.6410741158302934E-5 240-249 0.0 250-259 3.282148231660587E-5 260-269 6.564296463321173E-5 270-279 1.6410741158302934E-5 280-289 0.0 290-299 3.282148231660587E-5 300-309 0.0 310-319 1.6410741158302934E-5 320-329 0.0 330-339 0.0 340-349 3.282148231660587E-5 350-351 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 351 609357.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.468960482485313 #Duplication Level Percentage of deduplicated Percentage of total 1 70.60955318824408 4.567704092606958 2 9.869496674103653 1.2769076793359349 3 4.771909302792478 0.9260787811730573 4 2.6017418216180324 0.6732226011870561 5 1.5823810564327907 0.5118180261148543 6 1.1224689606776066 0.43567244096638796 7 0.8072907310509773 0.3655632285931816 8 0.7445920253056129 0.3853389109820571 9 0.5370903965425616 0.31269748956805776 >10 4.636918172461321 6.466038209130488 >50 1.0149323758422109 4.705935008723939 >100 1.2150833203041904 17.067439255468575 >500 0.23944997704037466 11.23316133852921 >1k 0.20888189486500766 29.327205676848113 >5k 0.030568082175366974 13.524516643250614 >10k+ 0.007642020543841744 8.220700617521516 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAACGTTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 20476 3.360263359574108 No Hit GAACGTTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG 18988 3.1160715311385605 No Hit GAACGTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA 10423 1.7104915509299146 No Hit GAACGTTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGT 9670 1.5869186700078937 No Hit GAACGTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA 9121 1.4968237010488104 No Hit GAACGTTCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC 8322 1.36570187919397 No Hit GAACGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC 7582 1.2442623946225284 No Hit GAACGTTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT 6687 1.097386261255717 No Hit GAACGTTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT 6496 1.0660417456433586 No Hit GAACGTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 6459 1.0599697714147864 No Hit GAACGTTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG 5976 0.9807058916201832 No Hit GAACGTTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA 5846 0.9593719281143893 No Hit GAACGTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC 5534 0.9081704157004843 No Hit GAACGTTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA 5275 0.8656665961004797 No Hit GAACGTTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC 5105 0.8377683361313648 No Hit GAACGTTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACT 4890 0.8024852426410134 No Hit GAACGTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT 4877 0.8003518462904341 No Hit GAACGTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACT 4845 0.7951004091197771 No Hit GAACGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA 4530 0.7434065744711229 No Hit GAACGTTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 4312 0.7076311587460224 No Hit GAACGTTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG 4288 0.7036925808680298 No Hit GAACGTTGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTC 4025 0.660532331621693 No Hit GAACGTTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT 4020 0.6597117945637778 No Hit GAACGTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC 3930 0.6449421275213052 No Hit GAACGTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC 3683 0.604407596860297 No Hit GAACGTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT 3274 0.537287665522838 No Hit GAACGTTGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC 3223 0.5289181875321035 No Hit GAACGTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA 3182 0.5221897836571993 No Hit GAACGTTGCCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAAGACCTT 3131 0.5138203056664647 No Hit GAACGTTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT 3064 0.5028251090904019 No Hit GAACGTTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT 2990 0.4906811606332577 No Hit GAACGTTGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA 2987 0.4901888383985086 No Hit GAACGTTGAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAG 2908 0.4772243528834492 No Hit GAACGTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC 2835 0.4652445118378881 No Hit GAACGTTGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC 2751 0.4514594892649137 No Hit GAACGTTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA 2728 0.447685018798504 No Hit GAACGTTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG 2661 0.436689822222441 No Hit GAACGTTGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGC 2589 0.42487408858846293 No Hit GAACGTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTCCGAGCGT 2577 0.4229047996494666 No Hit GAACGTTATCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTC 2545 0.4176533624788096 No Hit GAACGTTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC 2543 0.4173251476556436 No Hit GAACGTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCA 2534 0.4158481809513963 No Hit GAACGTTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCT 2428 0.3984527953235952 No Hit GAACGTTCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCC 2425 0.39796047308884613 No Hit GAACGTTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGC 2417 0.3966476137961819 No Hit GAACGTTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC 2168 0.35578486831200756 No Hit GAACGTTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT 2155 0.3536514719614282 No Hit GAACGTTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC 2149 0.35266682749193 No Hit GAACGTTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC 2101 0.3447896717359446 No Hit GAACGTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT 2076 0.3406869864463689 No Hit GAACGTTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT 2074 0.3403587716232028 No Hit GAACGTTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG 2050 0.3364201937452101 No Hit GAACGTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA 2026 0.3324816158672174 No Hit GAACGTTGCGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCC 1953 0.32050177482165626 No Hit GAACGTTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT 1921 0.31525033765099936 No Hit GAACGTTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC 1884 0.3091783634224272 No Hit GAACGTTGTCCGTTTCCGAGCGTTATCCCCCACTACCAGGCAGATTCCTA 1840 0.301957637312774 No Hit GAACGTTAGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACAT 1816 0.29801905943478124 No Hit GAACGTTAGGGCTGGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGG 1763 0.2893213666208807 No Hit GAACGTTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC 1703 0.27947492192589896 No Hit GAACGTTGTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCC 1700 0.27898259969114986 No Hit GAACGTTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA 1692 0.27766974039848563 No Hit GAACGTTGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT 1672 0.274387592166825 No Hit GAACGTTGCCTTCGCCACTGGTGTTCCTTCCTATATCTACGCATTTCACC 1656 0.27176187358149656 No Hit GAACGTTGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATG 1618 0.26552579194134146 No Hit GAACGTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTA 1602 0.26290007335601295 No Hit GAACGTTCACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGC 1601 0.26273596594442994 No Hit GAACGTTGGCCGCGTCTGGCGCGACATGGAACGACATCGATGCCGGGCTT 1579 0.2591256028896033 No Hit GAACGTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT 1576 0.2586332806548542 No Hit GAACGTTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA 1543 0.2532177360726143 No Hit GAACGTTCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC 1487 0.24402772102396464 No Hit GAACGTTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT 1459 0.23943271349963977 No Hit GAACGTTCCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCC 1438 0.23598645785639616 No Hit GAACGTTGCCACTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGT 1430 0.2346735985637319 No Hit GAACGTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTT 1329 0.21809874999384596 No Hit GAACGTTACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCC 1316 0.21596535364326658 No Hit GAACGTTAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT 1299 0.21317552764635508 No Hit GAACGTTCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTA 1292 0.2120267757652739 No Hit GAACGTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTT 1279 0.20989337941469452 No Hit GAACGTTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA 1262 0.20710355341778303 No Hit GAACGTTGGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 1227 0.201359794012377 No Hit GAACGTTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC 1226 0.20119568660079395 No Hit GAACGTTGTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTAC 1214 0.1992263976617976 No Hit GAACGTTGGCTCAGCAATCGTTGGCTACATCTTTGATTTCTCGCGGAGTA 1176 0.1929903160216425 No Hit GAACGTTGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCT 1173 0.19249799378689342 No Hit GAACGTTGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT 1169 0.19184156414056128 No Hit GAACGTTCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCAT 1168 0.19167745672897823 No Hit GAACGTTGCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTG 1158 0.19003638261314795 No Hit GAACGTTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCC 1147 0.18823120108573463 No Hit GAACGTTCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTC 1132 0.1857695899119892 No Hit GAACGTTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGT 1114 0.18281565650349466 No Hit GAACGTTGCCGGTGCTTATTCTGTCGGTAACGTCAAAACAATCACGTATT 1114 0.18281565650349466 No Hit GAACGTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCG 1090 0.17887707862550198 No Hit GAACGTTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC 1063 0.17444617851276017 No Hit GAACGTTCACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTC 1050 0.1723127821621808 No Hit GAACGTTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT 1027 0.16853831169577113 No Hit GAACGTTGCCAGGGTGGTATTTCAAGGTTGGCTCCACGCAGACTGGCGTC 1022 0.16771777463785595 No Hit GAACGTTGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACT 987 0.16197401523244995 No Hit GAACGTTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA 981 0.16098937076295178 No Hit GAACGTTGCCTCTTGCACCTATGTATTCAGTACAAGATAACCATCTTATG 979 0.1606611559397857 No Hit GAACGTTCCGTCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAAT 979 0.1606611559397857 No Hit GAACGTTCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCT 975 0.1600047262934536 No Hit GAACGTTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT 975 0.1600047262934536 No Hit GAACGTTGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCCCC 960 0.15754311511970814 No Hit GAACGTTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC 954 0.15655847065020997 No Hit GAACGTTAGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC 948 0.1555738261807118 No Hit GAACGTTGCGTCCGTTTCCGAGCGTTATCCCCCACTACCAGGCAGATTCC 947 0.1554097187691288 No Hit GAACGTTGACCCATTATACAAAAGGTACGCAGTCACCCAACAAAGTGGGC 945 0.1550815039459627 No Hit GAACGTTGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC 945 0.1550815039459627 No Hit GAACGTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCAC 935 0.15344042983013242 No Hit GAACGTTACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCAC 930 0.15261989277221727 No Hit GAACGTTGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATA 912 0.14966595936372276 No Hit GAACGTTGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAA 912 0.14966595936372276 No Hit GAACGTTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 910 0.14933774454055668 No Hit GAACGTTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGG 907 0.14884542230580758 No Hit GAACGTTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC 906 0.14868131489422456 No Hit GAACGTTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCG 906 0.14868131489422456 No Hit GAACGTTGCTCCTCTACCGCATCATCAAAAGATGATACCCGTAGCTTCGG 886 0.14539916666256397 No Hit GAACGTTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCAC 879 0.14425041478148276 No Hit GAACGTTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA 873 0.1432657703119846 No Hit GAACGTTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT 872 0.14310166290040158 No Hit GAACGTTGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTAC 867 0.14228112584248642 No Hit GAACGTTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGC 858 0.14080415913823918 No Hit GAACGTTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT 858 0.14080415913823918 No Hit GAACGTTTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCG 853 0.139983622080324 No Hit GAACGTTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG 837 0.13735790349499555 No Hit GAACGTTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC 837 0.13735790349499555 No Hit GAACGTTGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC 832 0.1365373664370804 No Hit GAACGTTCCCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGA 815 0.1337475404401689 No Hit GAACGTTTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGAC 812 0.13325521820541983 No Hit GAACGTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT 811 0.1330911107938368 No Hit GAACGTTGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGA 809 0.13276289597067073 No Hit GAACGTTACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGCC 792 0.12997306997375924 No Hit GAACGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCG 778 0.1276755662115968 No Hit GAACGTTACCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 763 0.12521395503785138 No Hit GAACGTTAAGGGCTGGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCG 758 0.12439341797993622 No Hit GAACGTTCTTGCACCTATGTATTCAGTACAAGATAACCATCTTATGATGG 755 0.12390109574518715 No Hit GAACGTTGGCCGTCCTTTCCAGAACGTTCCGCTAATCTCAATACAACTTA 749 0.12291645127568897 No Hit GAACGTTGCCCTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGGTT 745 0.12226002162935684 No Hit GAACGTTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCTGACGA 734 0.12045484010194353 No Hit GAACGTTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG 733 0.12029073269036049 No Hit GAACGTTGGCTCATCTGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCTC 710 0.11651626222395081 No Hit GAACGTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACC 709 0.1163521548123678 No Hit GAACGTTGCCGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGA 700 0.11487518810812053 No Hit GAACGTTCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCC 694 0.11389054363862236 No Hit GAACGTTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC 693 0.11372643622703932 No Hit GAACGTTCCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 691 0.11339822140387326 No Hit GAACGTTGAGCCGTTTCCTCAGGTGCAATCAGAGCAGCCGCGCCACTCAC 688 0.11290589916912418 No Hit GAACGTTTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTT 685 0.11241357693437509 No Hit GAACGTTCACCTGTCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAA 684 0.11224946952279205 No Hit GAACGTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA 679 0.11142893246487691 No Hit GAACGTTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT 672 0.1102801805837957 No Hit GAACGTTCGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTG 667 0.10945964352588056 No Hit GAACGTTGTCGCCTTCGCCACTGGTGTTCCTTCCTATATCTACGCATTTC 666 0.10929553611429754 No Hit GAACGTTGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCC 665 0.1091314287027145 No Hit GAACGTTCCCGCTTAGATGCTTTCAGCGGTTATCTTTTCCGAACATAGCT 655 0.1074903545868842 No Hit GAACGTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG 643 0.10552106564788787 No Hit GAACGTTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA 622 0.10207481000464425 No Hit GAACGTTCGCCTTCGCCACTGGTGTTCCTTCCTATATCTACGCATTTCAC 620 0.10174659518147819 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.016738955981468993 0.0 1.6410741158302933E-4 9 0.6145822563784448 0.0 1.6410741158302933E-4 10-19 0.6596789730814613 0.0 1.6410741158302933E-4 20-29 0.6871669645216187 0.0 1.6410741158302933E-4 30-39 0.690268594600538 0.0 1.6410741158302933E-4 40-49 0.7570439003736726 0.0 1.6410741158302933E-4 50-59 1.2028745054212884 0.0 1.6410741158302933E-4 60-69 2.1353164072949022 0.0 1.6410741158302933E-4 70-79 3.7265511022274302 0.0 1.6410741158302933E-4 80-89 5.70949377786749 0.0 1.6410741158302933E-4 90-99 9.195430593231881 0.0 1.6410741158302933E-4 100-109 13.028733566694076 0.0 1.6410741158302933E-4 110-119 17.648291559791716 0.0 1.6410741158302933E-4 120-129 21.845765290297805 0.0 1.6410741158302933E-4 130-139 27.520566761356644 0.0 1.6410741158302933E-4 140-149 31.34807674318995 0.0 1.6410741158302933E-4 150-159 34.736665042003295 0.0 3.2821482316605866E-4 160-169 38.101047497608135 0.0 3.2821482316605866E-4 170-179 41.83572191670892 0.0 3.2821482316605866E-4 180-189 45.17274766680287 0.0 4.266792701158763E-4 190-199 48.57651590118765 0.0 5.415544582239968E-4 200-209 51.772803135107985 0.0 6.564296463321173E-4 210-219 53.78994579532194 0.0 6.564296463321173E-4 220-229 55.64547547660895 0.0 7.384833521236319E-4 230-239 57.89220440562756 0.0 8.205370579151465E-4 240-249 59.69723823637047 0.0 9.682337283398732E-4 250-259 61.12899334872661 0.0 9.84644469498176E-4 260-269 62.45718027363271 0.0 0.0010666981752896908 270-279 63.77491355642095 0.0 0.0012636270691893257 280-289 64.92743990796856 0.0 0.0013128592926642346 290-299 66.0910599205392 0.0 0.0013128592926642346 300-309 67.22694578055228 0.0 0.0014769667042472642 310-319 68.08865738803362 0.0 0.0016246633746719902 320-329 68.89934800125377 0.0 0.001641074115830293 330-339 69.56541075264582 0.0 0.001641074115830293 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGCC 12020 0.0 341.5546 4 GTTGCCC 6535 0.0 340.24753 5 GTTGCCT 2515 0.0 338.8259 5 CGTTCCC 9985 0.0 336.5337 4 ACGTTGG 4125 0.0 336.21704 3 GTTGTCC 2325 0.0 333.86987 5 GTTAGGG 435 0.0 333.10236 5 CGTTGTC 3165 0.0 332.46335 4 ACGTTGT 4045 0.0 332.20532 3 GTTGGCC 2090 0.0 331.79315 5 TTGGCCC 1060 0.0 330.35266 6 AACGTTT 4740 0.0 329.3502 2 GTTGCGG 795 0.0 327.6404 5 CGTTCGG 1400 0.0 326.51678 4 AACGTTA 7550 0.0 326.2638 2 TGGCCCG 575 0.0 323.99893 7 TTGCCCC 4200 0.0 322.82037 6 CGTTGGG 680 0.0 322.16803 4 GTTGGGC 450 0.0 321.99893 5 GTTGCCG 2745 0.0 319.86234 5 >>END_MODULE