FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 ff.3410000000ebb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 ff.3410000000ebb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609357
Sequences flagged as poor quality0
Sequence length351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGTTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT204763.360263359574108No Hit
GAACGTTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG189883.1160715311385605No Hit
GAACGTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA104231.7104915509299146No Hit
GAACGTTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGT96701.5869186700078937No Hit
GAACGTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA91211.4968237010488104No Hit
GAACGTTCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC83221.36570187919397No Hit
GAACGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC75821.2442623946225284No Hit
GAACGTTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT66871.097386261255717No Hit
GAACGTTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT64961.0660417456433586No Hit
GAACGTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA64591.0599697714147864No Hit
GAACGTTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG59760.9807058916201832No Hit
GAACGTTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA58460.9593719281143893No Hit
GAACGTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC55340.9081704157004843No Hit
GAACGTTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA52750.8656665961004797No Hit
GAACGTTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC51050.8377683361313648No Hit
GAACGTTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACT48900.8024852426410134No Hit
GAACGTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT48770.8003518462904341No Hit
GAACGTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACT48450.7951004091197771No Hit
GAACGTTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA45300.7434065744711229No Hit
GAACGTTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA43120.7076311587460224No Hit
GAACGTTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG42880.7036925808680298No Hit
GAACGTTGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTC40250.660532331621693No Hit
GAACGTTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT40200.6597117945637778No Hit
GAACGTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC39300.6449421275213052No Hit
GAACGTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC36830.604407596860297No Hit
GAACGTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT32740.537287665522838No Hit
GAACGTTGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC32230.5289181875321035No Hit
GAACGTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA31820.5221897836571993No Hit
GAACGTTGCCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAAGACCTT31310.5138203056664647No Hit
GAACGTTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT30640.5028251090904019No Hit
GAACGTTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT29900.4906811606332577No Hit
GAACGTTGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA29870.4901888383985086No Hit
GAACGTTGAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAG29080.4772243528834492No Hit
GAACGTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC28350.4652445118378881No Hit
GAACGTTGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC27510.4514594892649137No Hit
GAACGTTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA27280.447685018798504No Hit
GAACGTTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG26610.436689822222441No Hit
GAACGTTGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGC25890.42487408858846293No Hit
GAACGTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTCCGAGCGT25770.4229047996494666No Hit
GAACGTTATCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTC25450.4176533624788096No Hit
GAACGTTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC25430.4173251476556436No Hit
GAACGTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCA25340.4158481809513963No Hit
GAACGTTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCT24280.3984527953235952No Hit
GAACGTTCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCC24250.39796047308884613No Hit
GAACGTTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGC24170.3966476137961819No Hit
GAACGTTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC21680.35578486831200756No Hit
GAACGTTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT21550.3536514719614282No Hit
GAACGTTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC21490.35266682749193No Hit
GAACGTTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC21010.3447896717359446No Hit
GAACGTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT20760.3406869864463689No Hit
GAACGTTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT20740.3403587716232028No Hit
GAACGTTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG20500.3364201937452101No Hit
GAACGTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA20260.3324816158672174No Hit
GAACGTTGCGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCC19530.32050177482165626No Hit
GAACGTTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT19210.31525033765099936No Hit
GAACGTTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC18840.3091783634224272No Hit
GAACGTTGTCCGTTTCCGAGCGTTATCCCCCACTACCAGGCAGATTCCTA18400.301957637312774No Hit
GAACGTTAGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACAT18160.29801905943478124No Hit
GAACGTTAGGGCTGGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGG17630.2893213666208807No Hit
GAACGTTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC17030.27947492192589896No Hit
GAACGTTGTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCC17000.27898259969114986No Hit
GAACGTTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA16920.27766974039848563No Hit
GAACGTTGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT16720.274387592166825No Hit
GAACGTTGCCTTCGCCACTGGTGTTCCTTCCTATATCTACGCATTTCACC16560.27176187358149656No Hit
GAACGTTGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATG16180.26552579194134146No Hit
GAACGTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTA16020.26290007335601295No Hit
GAACGTTCACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGC16010.26273596594442994No Hit
GAACGTTGGCCGCGTCTGGCGCGACATGGAACGACATCGATGCCGGGCTT15790.2591256028896033No Hit
GAACGTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT15760.2586332806548542No Hit
GAACGTTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA15430.2532177360726143No Hit
GAACGTTCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC14870.24402772102396464No Hit
GAACGTTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT14590.23943271349963977No Hit
GAACGTTCCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCC14380.23598645785639616No Hit
GAACGTTGCCACTACTAAGGGAATCTCGGTTGATTTCTTTTCCTCAGGGT14300.2346735985637319No Hit
GAACGTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTT13290.21809874999384596No Hit
GAACGTTACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCC13160.21596535364326658No Hit
GAACGTTAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT12990.21317552764635508No Hit
GAACGTTCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTA12920.2120267757652739No Hit
GAACGTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTT12790.20989337941469452No Hit
GAACGTTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA12620.20710355341778303No Hit
GAACGTTGGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA12270.201359794012377No Hit
GAACGTTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC12260.20119568660079395No Hit
GAACGTTGTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTAC12140.1992263976617976No Hit
GAACGTTGGCTCAGCAATCGTTGGCTACATCTTTGATTTCTCGCGGAGTA11760.1929903160216425No Hit
GAACGTTGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCT11730.19249799378689342No Hit
GAACGTTGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT11690.19184156414056128No Hit
GAACGTTCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCAT11680.19167745672897823No Hit
GAACGTTGCCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTG11580.19003638261314795No Hit
GAACGTTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCC11470.18823120108573463No Hit
GAACGTTCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTC11320.1857695899119892No Hit
GAACGTTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGT11140.18281565650349466No Hit
GAACGTTGCCGGTGCTTATTCTGTCGGTAACGTCAAAACAATCACGTATT11140.18281565650349466No Hit
GAACGTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCG10900.17887707862550198No Hit
GAACGTTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC10630.17444617851276017No Hit
GAACGTTCACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTC10500.1723127821621808No Hit
GAACGTTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT10270.16853831169577113No Hit
GAACGTTGCCAGGGTGGTATTTCAAGGTTGGCTCCACGCAGACTGGCGTC10220.16771777463785595No Hit
GAACGTTGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACT9870.16197401523244995No Hit
GAACGTTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA9810.16098937076295178No Hit
GAACGTTGCCTCTTGCACCTATGTATTCAGTACAAGATAACCATCTTATG9790.1606611559397857No Hit
GAACGTTCCGTCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAAT9790.1606611559397857No Hit
GAACGTTCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCT9750.1600047262934536No Hit
GAACGTTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT9750.1600047262934536No Hit
GAACGTTGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCCCC9600.15754311511970814No Hit
GAACGTTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC9540.15655847065020997No Hit
GAACGTTAGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC9480.1555738261807118No Hit
GAACGTTGCGTCCGTTTCCGAGCGTTATCCCCCACTACCAGGCAGATTCC9470.1554097187691288No Hit
GAACGTTGACCCATTATACAAAAGGTACGCAGTCACCCAACAAAGTGGGC9450.1550815039459627No Hit
GAACGTTGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC9450.1550815039459627No Hit
GAACGTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCAC9350.15344042983013242No Hit
GAACGTTACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCAC9300.15261989277221727No Hit
GAACGTTGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCATA9120.14966595936372276No Hit
GAACGTTGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAA9120.14966595936372276No Hit
GAACGTTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA9100.14933774454055668No Hit
GAACGTTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGG9070.14884542230580758No Hit
GAACGTTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC9060.14868131489422456No Hit
GAACGTTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCG9060.14868131489422456No Hit
GAACGTTGCTCCTCTACCGCATCATCAAAAGATGATACCCGTAGCTTCGG8860.14539916666256397No Hit
GAACGTTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCAC8790.14425041478148276No Hit
GAACGTTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA8730.1432657703119846No Hit
GAACGTTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT8720.14310166290040158No Hit
GAACGTTGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTAC8670.14228112584248642No Hit
GAACGTTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGC8580.14080415913823918No Hit
GAACGTTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT8580.14080415913823918No Hit
GAACGTTTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCG8530.139983622080324No Hit
GAACGTTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG8370.13735790349499555No Hit
GAACGTTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC8370.13735790349499555No Hit
GAACGTTGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC8320.1365373664370804No Hit
GAACGTTCCCCCTACAGTGATACATGAGGCGCTACCTAAATAGCTTTCGA8150.1337475404401689No Hit
GAACGTTTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGAC8120.13325521820541983No Hit
GAACGTTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT8110.1330911107938368No Hit
GAACGTTGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGA8090.13276289597067073No Hit
GAACGTTACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGCC7920.12997306997375924No Hit
GAACGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCG7780.1276755662115968No Hit
GAACGTTACCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT7630.12521395503785138No Hit
GAACGTTAAGGGCTGGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCG7580.12439341797993622No Hit
GAACGTTCTTGCACCTATGTATTCAGTACAAGATAACCATCTTATGATGG7550.12390109574518715No Hit
GAACGTTGGCCGTCCTTTCCAGAACGTTCCGCTAATCTCAATACAACTTA7490.12291645127568897No Hit
GAACGTTGCCCTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGGTT7450.12226002162935684No Hit
GAACGTTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCTGACGA7340.12045484010194353No Hit
GAACGTTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG7330.12029073269036049No Hit
GAACGTTGGCTCATCTGATAGCGCAAGGCCCGAAGGTCCCCTGCTTTCTC7100.11651626222395081No Hit
GAACGTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACC7090.1163521548123678No Hit
GAACGTTGCCGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGA7000.11487518810812053No Hit
GAACGTTCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCC6940.11389054363862236No Hit
GAACGTTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC6930.11372643622703932No Hit
GAACGTTCCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT6910.11339822140387326No Hit
GAACGTTGAGCCGTTTCCTCAGGTGCAATCAGAGCAGCCGCGCCACTCAC6880.11290589916912418No Hit
GAACGTTTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTT6850.11241357693437509No Hit
GAACGTTCACCTGTCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAA6840.11224946952279205No Hit
GAACGTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA6790.11142893246487691No Hit
GAACGTTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT6720.1102801805837957No Hit
GAACGTTCGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTG6670.10945964352588056No Hit
GAACGTTGTCGCCTTCGCCACTGGTGTTCCTTCCTATATCTACGCATTTC6660.10929553611429754No Hit
GAACGTTGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCC6650.1091314287027145No Hit
GAACGTTCCCGCTTAGATGCTTTCAGCGGTTATCTTTTCCGAACATAGCT6550.1074903545868842No Hit
GAACGTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG6430.10552106564788787No Hit
GAACGTTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA6220.10207481000464425No Hit
GAACGTTCGCCTTCGCCACTGGTGTTCCTTCCTATATCTACGCATTTCAC6200.10174659518147819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCC120200.0341.55464
GTTGCCC65350.0340.247535
GTTGCCT25150.0338.82595
CGTTCCC99850.0336.53374
ACGTTGG41250.0336.217043
GTTGTCC23250.0333.869875
GTTAGGG4350.0333.102365
CGTTGTC31650.0332.463354
ACGTTGT40450.0332.205323
GTTGGCC20900.0331.793155
TTGGCCC10600.0330.352666
AACGTTT47400.0329.35022
GTTGCGG7950.0327.64045
CGTTCGG14000.0326.516784
AACGTTA75500.0326.26382
TGGCCCG5750.0323.998937
TTGCCCC42000.0322.820376
CGTTGGG6800.0322.168034
GTTGGGC4500.0321.998935
GTTGCCG27450.0319.862345
Produced by FastQC (version 0.11.2)