FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 ca73.3410000000eb16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 ca73.3410000000eb16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417842
Sequences flagged as poor quality0
Sequence length351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT193674.635005576270456No Hit
GCCAATTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA143703.4390989895702204No Hit
GCCAATTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG107222.566041709545713No Hit
GCCAATTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT86402.0677672421633058No Hit
GCCAATTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT79901.912206049176483No Hit
GCCAATTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA79811.9100521249658962No Hit
GCCAATTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC67001.6034769123257118No Hit
GCCAATTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA64781.5503467817979046No Hit
GCCAATTCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC63901.5292861895166117No Hit
GCCAATTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGT63311.5151660196916539No Hit
GCCAATTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG53461.2794309810885454No Hit
GCCAATTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA51171.2246255761747264No Hit
GCCAATTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC46371.1097496182767648No Hit
GCCAATTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC42731.022635350204144No Hit
GCCAATTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC37210.8905279986214885No Hit
GCCAATTGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTC30750.7359241052838154No Hit
GCCAATTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG28180.6744176028259486No Hit
GCCAATTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT27540.6591008084395538No Hit
GCCAATTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACT25970.6215267972104288No Hit
GCCAATTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC24930.5966370063325372No Hit
GCCAATTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT24220.5796449375601304No Hit
GCCAATTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT21710.519574384575988No Hit
GCCAATTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC19810.4741026512413783No Hit
GCCAATTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC19600.4690768280833425No Hit
GCCAATTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA19400.46429032983759416No Hit
GCCAATTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC18870.4516061094863608No Hit
GCCAATTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT18720.4480162358020495No Hit
GCCAATTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC18510.4429904126440138No Hit
GCCAATTGTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTAC18410.44059716352113953No Hit
GCCAATTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA18370.43963986387198994No Hit
GCCAATTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA16360.3915355565022185No Hit
GCCAATTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCG15940.3814839101861469No Hit
GCCAATTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC15830.3788513361509853No Hit
GCCAATTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA15610.3735861880806621No Hit
GCCAATTGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC15360.3676030652734766No Hit
GCCAATTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT14250.341038000009573No Hit
GCCAATTCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCC13930.3333796028163756No Hit
GCCAATTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC13890.3324223031672259No Hit
GCCAATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTT13270.31758415860540584No Hit
GCCAATTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT13180.3154302343948191No Hit
GCCAATTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGC12990.31088306106135816No Hit
GCCAATTGAGGGATGTCCACCTGCGGGAAGCGTCAACTCGAGGAAACGAA12560.30059208983299907No Hit
GCCAATTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA12500.2991561403592745No Hit
GCCAATTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACT12440.2977201908855501No Hit
GCCAATTCCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT12010.287429219657191No Hit
GCCAATTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC11990.2869505698326162No Hit
GCCAATTCCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCC11850.2836000210605923No Hit
GCCAATTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT11840.2833606961483049No Hit
GCCAATTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC11560.2766595986042571No Hit
GCCAATTACCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT11470.27450567439367035No Hit
GCCAATTGAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAG11430.27354837474452065No Hit
GCCAATTGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGC11290.2701978259724968No Hit
GCCAATTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCA11190.2678045768496226No Hit
GCCAATTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT11090.26541132772674836No Hit
GCCAATTTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTC10560.2527271073755151No Hit
GCCAATTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC10550.25248778246322773No Hit
GCCAATTGCCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAAGACCTT10490.25105183298950323No Hit
GCCAATTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGT10450.25009453334035353No Hit
GCCAATTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCT9980.2388462624628448No Hit
GCCAATTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG9970.2386069375505574No Hit
GCCAATTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCA9850.23573503860310838No Hit
GCCAATTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA9590.22951259088363546No Hit
GCCAATTGGCCAGATTTTCTTAAAGATCTTCTTCAAGACGGAGATGACCT9540.22831596632219833No Hit
GCCAATTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTCCGAGCGT9390.22472609263788704No Hit
GCCAATTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT9330.22329014316416254No Hit
GCCAATTCACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGC9310.2228114933395877No Hit
GCCAATTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG9190.21993959439213867No Hit
GCCAATTGTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCG8990.21515309614639025No Hit
GCCAATTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCG8960.21443512140952803No Hit
GCCAATTCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCC8910.2132384968480909No Hit
GCCAATTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCG8850.2118025473743664No Hit
GCCAATTGGCCGCGTCTGGCGCGACATGGAATGACATCGAGGCCGGGCTT8580.20534077474260604No Hit
GCCAATTCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTC8240.19720372772483377No Hit
GCCAATTATCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTC8240.19720372772483377No Hit
GCCAATTGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTAC8000.1914599298299357No Hit
GCCAATTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC7990.1912206049176483No Hit
GCCAATTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA7980.19098128000536088No Hit
GCCAATTCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC7800.1866734315841873No Hit
GCCAATTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA7570.18116895860157667No Hit
GCCAATTCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTG7500.17949368421556472No Hit
GCCAATTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT7490.17925435930327732No Hit
GCCAATTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCAC7460.17853638456641505No Hit
GCCAATTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT7440.17805773474184022No Hit
GCCAATTCCGTCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAAT7440.17805773474184022No Hit
GCCAATTCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTC7320.17518583579439118No Hit
GCCAATTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCAC7240.17327123649609183No Hit
GCCAATTGCCGGTGGAGCGATCACCCGATTGAGTGAAAGCCTTCGAGGTT7220.17279258667151698No Hit
GCCAATTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG7210.17255326175922955No Hit
GCCAATTCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCT7190.17207461193465473No Hit
GCCAATTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC7130.1706386624609302No Hit
GCCAATTCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG7100.16992068772406793No Hit
GCCAATTTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT7030.168245413338056No Hit
GCCAATTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT7020.16800608842576858No Hit
GCCAATTGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC6830.16345891509230762No Hit
GCCAATTTCCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCC6730.16106566596943342No Hit
GCCAATTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA6690.16010836632028375No Hit
GCCAATTACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTA6370.1524499691270863No Hit
GCCAATTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCT6350.15197131930251148No Hit
GCCAATTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG6270.15005672000421214No Hit
GCCAATTCGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAA6260.1498173950919247No Hit
GCCAATTGCCGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGA5980.14311629754787694No Hit
GCCAATTCACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCGTATG5930.14191967298643984No Hit
GCCAATTCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC5820.13928709895127822No Hit
GCCAATTGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC5760.13785114947755372No Hit
GCCAATTCGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT5760.13785114947755372No Hit
GCCAATTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG5720.13689384982840405No Hit
GCCAATTCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGT5700.1364152000038292No Hit
GCCAATTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT5660.13545790035467953No Hit
GCCAATTGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACT5530.13234667649494306No Hit
GCCAATTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTA5460.13067140210893113No Hit
GCCAATTGCGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCC5390.12899612772291918No Hit
GCCAATTGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT5320.12732085333690724No Hit
GCCAATTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT5290.126602878600045No Hit
GCCAATTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT5270.12612422877547014No Hit
GCCAATTTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCG5110.12229503017887144No Hit
GCCAATTGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATG5080.12157705544200918No Hit
GCCAATTGCCTCTGACTGCCAAGGCATCCACCGTATGCGCTAATTGGCAG5060.12109840561743435No Hit
GCCAATTCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGTCCCCCCAT5040.12061975579285951No Hit
GCCAATTCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCT5000.11966245614370982No Hit
GCCAATTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT4940.11822650666998531No Hit
GCCAATTTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGG4900.11726920702083563No Hit
GCCAATTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGC4830.1155939326348237No Hit
GCCAATTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG4820.11535460772253626No Hit
GCCAATTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC4810.11511528281024884No Hit
GCCAATTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTC4800.11487595789796143No Hit
GCCAATTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA4750.11367933333652433No Hit
GCCAATTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG4740.11344000842423692No Hit
GCCAATTGTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCC4680.11200405895051238No Hit
GCCAATTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA4660.11152540912593754No Hit
GCCAATTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC4590.10985013473992562No Hit
GCCAATTGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTCT4510.10793553544162626No Hit
GCCAATTCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT4500.10769621052933885No Hit
GCCAATTTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA4450.10649958596790174No Hit
GCCAATTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGG4440.10626026105561431No Hit
GCCAATTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACC4410.10554228631875207No Hit
GCCAATTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCTGACGA4350.10410633684502756No Hit
GCCAATTCACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTC4320.1033883621081653No Hit
GCCAATTCGGCTTGCACTAGGGGTTTTCACCCTATCCTCCCCGTAGGGAT4270.10219173754672818No Hit
GCCAATTGGCGTCGGGACCATCCTCATCCTGCAAGCGTCGCCAATTAAGG4220.1009951129852911No Hit
GCCAATTACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCC4210.10075578807300367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCC71500.0339.933564
CCAATTC182700.0338.579622
AATTAGG4850.0337.886634
CAATTGC91950.0336.74553
ATTGCCC43900.0335.962435
CAATTCG34250.0333.416053
AATTCCC73850.0333.32094
CAATTCC103000.0333.27673
GCCAATT420850.0332.088651
AATTCGG11350.0331.32164
AATTGCG10950.0330.821934
CCAATTT36950.0330.527742
ATTGCCG15500.0328.306465
TTAGGGC950.0326.84216
AATTCGC19850.0325.881624
ATTGCGC6200.0325.524175
CAATTGT31650.0323.199073
TTGCCCC32250.0322.534886
ATTGGGC2800.0320.357155
ATTCGCC15850.0319.968445
Produced by FastQC (version 0.11.2)