##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AGWYV l01n01 ca66c.3410000000eb5a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1014539 Sequences flagged as poor quality 0 Sequence length 351 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.611220465649915 32.0 32.0 33.0 29.0 34.0 2 32.32333404630083 33.0 32.0 34.0 32.0 34.0 3 31.894594490699717 33.0 32.0 33.0 29.0 34.0 4 32.3920233721917 33.0 32.0 33.0 32.0 34.0 5 31.96690812280257 33.0 32.0 34.0 30.0 34.0 6 35.82806082368445 37.0 37.0 37.0 35.0 37.0 7 36.71683395118374 37.0 37.0 37.0 37.0 37.0 8 36.1619415320653 37.0 37.0 37.0 35.0 37.0 9 35.6558515739661 37.0 37.0 37.0 33.0 37.0 10-19 35.92554716969973 37.3 36.0 37.7 33.4 37.7 20-29 36.66218686516733 38.0 37.0 38.7 33.9 38.7 30-39 36.79131388739122 38.0 37.0 39.0 34.0 39.0 40-49 36.77778695545465 38.0 37.0 39.0 33.5 39.0 50-59 36.33025383942855 38.0 37.0 39.0 31.5 39.0 60-69 36.25587286442414 38.0 36.7 39.0 32.4 39.0 70-79 36.21312418743883 38.0 37.0 39.0 32.1 39.0 80-89 35.27627888134414 38.0 36.3 39.0 23.5 39.0 90-99 34.4483721177796 38.0 36.0 39.0 17.1 39.0 100-109 34.43832026171492 38.0 35.6 39.0 17.0 39.0 110-119 34.19793926108311 38.0 34.5 39.0 16.9 39.0 120-129 33.8172289088936 38.0 33.6 39.0 16.0 39.0 130-139 33.30779763025374 38.0 32.0 39.0 16.0 39.0 140-149 32.23707368568384 37.8 27.2 39.0 15.1 39.0 150-159 31.740726872007876 37.9 26.6 39.0 15.0 39.0 160-169 30.951917964711065 37.3 21.1 38.9 14.3 39.0 170-179 29.875004608004225 36.6 15.3 38.1 13.9 39.0 180-189 29.06106724334895 35.8 15.0 38.0 13.4 39.0 190-199 27.860286691788094 33.3 15.0 38.0 13.1 38.3 200-209 26.467217524412558 28.2 14.7 37.0 13.0 38.0 210-219 25.375107610451643 25.5 14.0 37.0 13.0 37.9 220-229 24.361722910602744 24.0 14.0 37.0 13.0 37.0 230-239 23.22120105782035 18.7 14.0 36.3 13.0 37.0 240-249 22.162371973871878 15.0 14.0 33.6 13.0 37.0 250-259 21.317888814525613 15.0 14.0 30.9 13.0 37.0 260-269 20.720515327651277 14.6 14.0 26.1 13.0 37.0 270-279 20.11879572889756 14.0 14.0 26.0 13.0 37.0 280-289 19.730022305697464 14.0 14.0 25.2 13.0 37.0 290-299 19.315931373756946 14.0 14.0 24.9 13.0 36.5 300-309 18.801899877678434 14.0 14.0 24.0 13.0 33.0 310-319 18.243517203380055 14.0 14.0 24.0 13.0 31.5 320-329 17.950149673891296 14.0 14.0 24.0 13.0 27.1 330-339 17.52763343745287 14.0 14.0 24.0 13.0 26.0 340-349 17.439319927573017 14.0 14.0 24.0 13.0 26.0 350-351 17.345876797244856 14.0 14.0 24.0 13.0 26.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33668937732664617 1101 2 0.13785908284721415 1101 3 0.6123700012030646 1101 4 0.31885491866298565 1101 5 0.4183152938992549 1101 6 0.10780216678956833 1101 7 0.08912212430782063 1101 8 0.0861695114750276 1101 9 0.14501394320376448 1101 10-19 0.15200002409929425 1101 20-29 0.16995920037914658 1101 30-39 0.1541381224208962 1101 40-49 0.1917765184555762 1101 50-59 0.08206304467199033 1101 60-69 0.16681003997307187 1101 70-79 0.19571514007795088 1101 80-89 -0.3345031879192959 1101 90-99 -0.5535733334363044 1101 100-109 -0.485128196610745 1101 110-119 -0.5386669561861055 1101 120-129 -0.6058310632125128 1101 130-139 -0.704387390141207 1101 140-149 -0.4200782500352638 1101 150-159 -0.6827957026049916 1101 160-169 -0.7801175349068856 1101 170-179 -0.71022936629344 1101 180-189 -0.9034481200310367 1101 190-199 -0.679759835140409 1101 200-209 -0.5042129765385148 1101 210-219 -0.5094390560877962 1101 220-229 -0.3267889457707369 1101 230-239 -0.3369474788601394 1101 240-249 -0.29166176315064263 1101 250-259 -0.2748356890856236 1101 260-269 -0.1463570146521711 1101 270-279 -0.07692802748728056 1101 280-289 -0.07896848782392496 1101 290-299 -0.019428167865225277 1101 300-309 -0.006564067145252039 1101 310-319 0.028965674972173616 1101 320-329 -0.0048069871241231965 1101 330-339 -0.05525756040046659 1101 340-349 -0.02457665575269985 1101 350-351 0.06992918914555801 1102 1 -0.5818190106848391 1102 2 0.05009953586377236 1102 3 -1.196846679542567 1102 4 -0.30305127979458746 1102 5 -0.4739635453939286 1102 6 -0.21304854028954168 1102 7 0.02255436660554011 1102 8 0.04935093068743868 1102 9 0.13629000757183007 1102 10-19 0.19928896238017302 1102 20-29 0.22941403126468884 1102 30-39 0.33522554362274093 1102 40-49 0.30671018983409937 1102 50-59 0.17025494156069954 1102 60-69 0.313653542970151 1102 70-79 0.3380978712776539 1102 80-89 -0.03528357889841516 1102 90-99 -0.012754998608848211 1102 100-109 -0.05784846683694411 1102 110-119 -0.7496717295372264 1102 120-129 -0.25721643735873556 1102 130-139 -0.21864452120382083 1102 140-149 0.48299853864037345 1102 150-159 0.22917797366274328 1102 160-169 0.013052152457550648 1102 170-179 0.26068125232252015 1102 180-189 0.12269968325635006 1102 190-199 0.1682699230350515 1102 200-209 0.31556590681002206 1102 210-219 -0.09276144494550209 1102 220-229 0.1004952757748434 1102 230-239 0.3522010904280677 1102 240-249 0.40603727989200067 1102 250-259 0.10553496832154252 1102 260-269 0.1619797512496568 1102 270-279 0.152108404981103 1102 280-289 0.24188029956642154 1102 290-299 0.1052566601070346 1102 300-309 0.16087888866553257 1102 310-319 0.2620088353497998 1102 320-329 0.017489823005529814 1102 330-339 0.04379228364356891 1102 340-349 0.015177864045774925 1102 350-351 0.12676476217827926 1103 1 -0.10425238711524543 1103 2 -0.09523613886269544 1103 3 -0.46875652304230897 1103 4 -0.2763323820504269 1103 5 0.25681046514234396 1103 6 0.31872390717384036 1103 7 0.09813569463857874 1103 8 0.031943701957771964 1103 9 0.11097092843402834 1103 10-19 0.17150485941034077 1103 20-29 -0.17111911166964688 1103 30-39 -0.02985884301588726 1103 40-49 -0.30475219028206624 1103 50-59 -1.0118635818178419 1103 60-69 0.0339915140629401 1103 70-79 -0.15889385022521196 1103 80-89 -0.792723767239039 1103 90-99 -0.19092890494025028 1103 100-109 -0.2986576754589265 1103 110-119 -0.3642323516788295 1103 120-129 -0.19888367650887773 1103 130-139 -0.21004846941015387 1103 140-149 0.22545484013976136 1103 150-159 -0.18660396802113155 1103 160-169 -0.11041914296322375 1103 170-179 0.131977560768906 1103 180-189 -0.0067101312975914595 1103 190-199 -0.054284408220624414 1103 200-209 0.16189249255711147 1103 210-219 0.27936491309467115 1103 220-229 0.17332254657240043 1103 230-239 0.1107959072011866 1103 240-249 0.20080449781523768 1103 250-259 0.10752396891975735 1103 260-269 0.04896718877498074 1103 270-279 0.14065150526863235 1103 280-289 0.10465230319429253 1103 290-299 0.2122921476506967 1103 300-309 0.2614547169976795 1103 310-319 0.2220146245086312 1103 320-329 0.050848075451451535 1103 330-339 0.1039392896835949 1103 340-349 0.05099110578174759 1103 350-351 -0.017602000331127243 2112 1 -0.09675283432643411 2112 2 0.11721565177091975 2112 3 0.603292419750435 2112 4 0.41262329037631673 2112 5 0.09089010451123869 2112 6 0.2088895541989899 2112 7 0.07808274483355859 2112 8 -0.002543078479426697 2112 9 0.1483670859954742 2112 10-19 -0.008396396358676839 2112 20-29 0.09027361130986122 2112 30-39 -0.0017826382289385378 2112 40-49 0.012421864313481024 2112 50-59 -0.10615344622341638 2112 60-69 -0.14408061215794277 2112 70-79 -0.7966603702066095 2112 80-89 -0.7849255489284346 2112 90-99 -0.217801288754778 2112 100-109 -0.14452959417382516 2112 110-119 -0.004316570490331628 2112 120-129 -0.2943543931200878 2112 130-139 -0.14800475004411595 2112 140-149 0.3107230595236743 2112 150-159 -0.7290426643110663 2112 160-169 -0.30297160978015114 2112 170-179 0.13696893853714442 2112 180-189 -0.2411916883080707 2112 190-199 -0.2363584663938525 2112 200-209 0.024536047349428713 2112 210-219 -0.08116315387967532 2112 220-229 -0.2530315015833011 2112 230-239 -0.07448698070979631 2112 240-249 -0.004883026781374866 2112 250-259 -0.162660711413686 2112 260-269 -0.6113052880594729 2112 270-279 -0.019751056357073082 2112 280-289 -0.12198602817131388 2112 290-299 0.0426672326208255 2112 300-309 -0.0037987623053865605 2112 310-319 -0.07330146436117602 2112 320-329 0.13598284701597407 2112 330-339 0.058850455652606826 2112 340-349 0.024148488344092556 2112 350-351 0.08483547954436688 1113 1 0.11893704739220823 1113 2 -0.18190976753148647 1113 3 0.0915280291648557 1113 4 0.05325429487602662 1113 5 0.31258929332745566 1113 6 0.053786957787075096 1113 7 -0.005571964087224046 1113 8 0.04652056403160998 1113 9 0.31838294809514167 1113 10-19 -0.029361281703685904 1113 20-29 0.193507013112459 1113 30-39 0.1779389710201329 1113 40-49 0.100063023524676 1113 50-59 0.2044505248735291 1113 60-69 0.3406922945400339 1113 70-79 0.335359388676153 1113 80-89 0.0556308237564167 1113 90-99 0.22420536224358756 1113 100-109 0.2929633613035847 1113 110-119 0.40621903731910436 1113 120-129 0.37118168255769035 1113 130-139 0.5739731232656098 1113 140-149 0.5743422755580241 1113 150-159 -0.996071626118777 1113 160-169 0.21391602247593156 1113 170-179 0.3887110364830271 1113 180-189 0.16137548562478798 1113 190-199 0.445390398230483 1113 200-209 0.439785125921329 1113 210-219 0.3205063411949922 1113 220-229 0.09612695618938716 1113 230-239 0.00853192692511584 1113 240-249 0.07446672555122547 1113 250-259 0.18776387532532368 1113 260-269 -0.1365304478085072 1113 270-279 0.2427464188194115 1113 280-289 0.33651136191066655 1113 290-299 0.23455468318046613 1113 300-309 0.22219530651698705 1113 310-319 0.17556493151722563 1113 320-329 0.26019861487575113 1113 330-339 0.15569753023385147 1113 340-349 0.1618202331235743 1113 350-351 0.041199488385714744 2113 1 0.36149102078752193 2113 2 0.3011709111337666 2113 3 0.559226529461899 2113 4 0.28264173493043643 2113 5 0.5068607211412086 2113 6 0.10139492419212104 2113 7 0.04274117999153049 2113 8 0.038546281833127694 2113 9 0.25494732058587033 2113 10-19 -0.0060593256908347826 2113 20-29 0.16980608407932607 2113 30-39 0.0012708821771667544 2113 40-49 0.032419694671816046 2113 50-59 0.15620725520912515 2113 60-69 0.18164264777221462 2113 70-79 0.23191286810188672 2113 80-89 0.30883112842329297 2113 90-99 0.21452810106956832 2113 100-109 0.14579901034164067 2113 110-119 0.25741644281761467 2113 120-129 0.2727492827975766 2113 130-139 0.28968695011452184 2113 140-149 0.49089277516087293 2113 150-159 0.092029567661978 2113 160-169 0.1773372216169129 2113 170-179 0.3934308860576792 2113 180-189 0.2374473509813555 2113 190-199 0.3019065443513398 2113 200-209 0.23974901184887187 2113 210-219 0.25558711808926304 2113 220-229 0.05541987670139292 2113 230-239 -0.06326917280055966 2113 240-249 -0.09223122742500323 2113 250-259 -0.006824749063529367 2113 260-269 -0.06292220083877353 2113 270-279 0.014422080398144743 2113 280-289 -0.028967672469800476 2113 290-299 0.012917512942042464 2113 300-309 -0.0705800273910775 2113 310-319 0.020373198195521525 2113 320-329 -0.0942880741503096 2113 330-339 0.03363712001313601 2113 340-349 -0.05128783355039346 2113 350-351 -0.030315554620081286 1112 1 -0.0904972061260203 1112 2 -0.027407020253434666 1112 3 0.1984176161885678 1112 4 0.1793831658636975 1112 5 0.3957583362887931 1112 6 0.273092926649241 1112 7 0.055556918017245494 1112 8 0.09289205751605323 1112 9 0.20537030893270014 1112 10-19 0.1282824290957052 1112 20-29 0.09283802811727782 1112 30-39 0.16784348250204317 1112 40-49 0.18196519635021957 1112 50-59 0.277007076811671 1112 60-69 0.14724848089438325 1112 70-79 0.13076850108253524 1112 80-89 0.19529711022863694 1112 90-99 0.19835300568918512 1112 100-109 0.17571941527277346 1112 110-119 0.14644529271512852 1112 120-129 0.20969901634331478 1112 130-139 0.06393239291114128 1112 140-149 0.4061665434613957 1112 150-159 0.07359867986946256 1112 160-169 -0.03188720419015212 1112 170-179 0.1055503231785444 1112 180-189 -0.12089168339403145 1112 190-199 0.13574616430640774 1112 200-209 -0.14569793382763763 1112 210-219 -0.19463199622138205 1112 220-229 0.017862993254691872 1112 230-239 -0.05800468910295109 1112 240-249 0.1423127652242151 1112 250-259 0.10727716450274016 1112 260-269 0.19139974125008763 1112 270-279 0.1125072869967525 1112 280-289 0.19810506110095005 1112 290-299 0.07168187743759091 1112 300-309 0.06158468946807716 1112 310-319 0.08884326211741467 1112 320-329 0.1858986077927618 1112 330-339 0.08848470329283487 1112 340-349 0.03158583388002256 1112 350-351 0.14541953552728515 2114 1 0.014472961610106694 2114 2 0.13572105209449603 2114 3 0.12927618499039895 2114 4 0.06450455878650985 2114 5 0.3578638561957348 2114 6 0.30830062675055814 2114 7 0.09782304432592781 2114 8 0.15630156631563352 2114 9 0.18681989428299062 2114 10-19 0.06540577331124808 2114 20-29 0.17399248044194593 2114 30-39 0.12658752543048735 2114 40-49 0.10361385108397769 2114 50-59 0.00954863559437058 2114 60-69 0.15014783921927233 2114 70-79 0.20148710215573828 2114 80-89 0.06671442419914797 2114 90-99 0.10173343272208513 2114 100-109 0.09486853906729209 2114 110-119 0.12019477674829915 2114 120-129 0.01449150386629583 2114 130-139 -0.06112783448951831 2114 140-149 0.33169245437736805 2114 150-159 0.2091675567503941 2114 160-169 0.18221144866736694 2114 170-179 0.295595165386505 2114 180-189 0.0973802967928421 2114 190-199 0.21794118100497073 2114 200-209 -0.27864382297920187 2114 210-219 0.28577664950640624 2114 220-229 0.3715438067936603 2114 230-239 0.16649191489719328 2114 240-249 -0.0332902592795179 2114 250-259 0.13195013534592448 2114 260-269 0.2641600699678648 2114 270-279 0.3385913802085092 2114 280-289 0.30473551627750695 2114 290-299 0.2711045574631079 2114 300-309 0.20618054672350894 2114 310-319 0.22284435033836303 2114 320-329 0.09622469882807749 2114 330-339 0.20138181012611156 2114 340-349 0.03780351559416317 2114 350-351 0.11903580630667321 1114 1 0.3884134508971293 1114 2 0.26640102485367834 1114 3 0.4295564511122514 1114 4 0.14458470965874426 1114 5 0.09335811990781906 1114 6 0.05560273791405734 1114 7 0.035897327944766744 1114 8 0.04596053221222718 1114 9 -0.01385326827135458 1114 10-19 -0.10649951245542155 1114 20-29 0.10397781230846448 1114 30-39 0.07173944538438803 1114 40-49 0.11749933487045894 1114 50-59 -0.0725496932861347 1114 60-69 -0.03301078616707542 1114 70-79 0.19775694342558836 1114 80-89 0.04754238794790666 1114 90-99 0.012377782871389797 1114 100-109 -0.10425949278351254 1114 110-119 -0.19728518553363728 1114 120-129 -0.17994193947803439 1114 130-139 -0.34974798221857384 1114 140-149 0.023976492304967678 1114 150-159 -0.378348813934295 1114 160-169 -0.15573527457638292 1114 170-179 -0.020868766720990806 1114 180-189 -0.305252371235877 1114 190-199 -0.313956246833051 1114 200-209 -0.4661117364372167 1114 210-219 -0.23779766030552452 1114 220-229 -0.22641129824065231 1114 230-239 -0.4175255820905974 1114 240-249 -0.34795210641661356 1114 250-259 -0.25767399373364697 1114 260-269 -0.18299090703459697 1114 270-279 -0.09375787852689044 1114 280-289 -0.1440475525055689 1114 290-299 -0.16076798693418937 1114 300-309 0.058813738614126976 1114 310-319 -7.003612756619759E-4 1114 320-329 -0.11059755482586553 1114 330-339 0.006523776703723172 1114 340-349 -0.04468351882356458 1114 350-351 -0.20385433985079615 1109 1 0.35033508423693505 1109 2 0.12012036737616683 1109 3 0.5811046941718523 1109 4 0.35187617409930283 1109 5 0.4747872922368188 1109 6 0.2299353917676754 1109 7 0.08963365746006957 1109 8 0.05180810226334387 1109 9 0.13690548407446812 1109 10-19 -0.03909197023060784 1109 20-29 0.09285141290381915 1109 30-39 0.10777605639842847 1109 40-49 0.25905930704413294 1109 50-59 0.12968830257227637 1109 60-69 -0.024108307683931685 1109 70-79 0.20847589767394226 1109 80-89 -0.10924884999941753 1109 90-99 -0.36152987260004465 1109 100-109 -0.42461597696134135 1109 110-119 -0.26131199262611204 1109 120-129 -0.3191196072448079 1109 130-139 -0.3627991696902626 1109 140-149 -0.022727869307658466 1109 150-159 -0.4808153252913101 1109 160-169 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120-129 0.053738194483507584 2105 130-139 -0.08444387534414943 2105 140-149 -2.231492012599695 2105 150-159 0.11676719271443758 2105 160-169 -0.10054815813680307 2105 170-179 0.2893113606712987 2105 180-189 0.008002856850289675 2105 190-199 0.2646728023520879 2105 200-209 0.38834676941919355 2105 210-219 0.3283023840129182 2105 220-229 0.06883061380374045 2105 230-239 0.07351443364998289 2105 240-249 0.3642953978269716 2105 250-259 0.257742391336258 2105 260-269 0.22046871142943658 2105 270-279 -0.0021931234495760066 2105 280-289 0.06656593747728223 2105 290-299 -0.011921372007620334 2105 300-309 -0.09218584576027666 2105 310-319 -0.08940577565630292 2105 320-329 -0.046083985787628734 2105 330-339 -0.10983230836589897 2105 340-349 -0.00991052506628165 2105 350-351 0.043120437731825945 2104 1 0.23770489500413206 2104 2 -0.21688882532834697 2104 3 -0.15616365456415338 2104 4 -0.1375033183460559 2104 5 -0.22976627482591994 2104 6 -0.02015567957357689 2104 7 -0.05239301605222835 2104 8 1.1564223694193743E-4 2104 9 -0.057539775326048925 2104 10-19 -0.02418456961450488 2104 20-29 -0.09086435894108291 2104 30-39 -0.06457388981446854 2104 40-49 0.13661926743727548 2104 50-59 0.24743688454870494 2104 60-69 -0.09479934968677384 2104 70-79 0.11309706534301256 2104 80-89 0.27888571554749575 2104 90-99 0.281006678179871 2104 100-109 0.36284337710446835 2104 110-119 0.3653062045280535 2104 120-129 0.3435778158640801 2104 130-139 0.5009732619756662 2104 140-149 -1.6910744049293882 2104 150-159 0.6596701032940828 2104 160-169 0.35369919754913326 2104 170-179 0.6758217813861904 2104 180-189 0.352361329660404 2104 190-199 0.5508588537543098 2104 200-209 0.3537658934704062 2104 210-219 0.4253719733660688 2104 220-229 0.13794325130407614 2104 230-239 0.22462670358684989 2104 240-249 0.2070585380393588 2104 250-259 0.2169443627404064 2104 260-269 0.09821939915811484 2104 270-279 -0.09227790821065796 2104 280-289 0.027277606818348232 2104 290-299 -0.04270783615601559 2104 300-309 -0.041820779326446456 2104 310-319 -0.06148757777785718 2104 320-329 -0.08227191677577039 2104 330-339 -0.0845296677928502 2104 340-349 -0.02307959409193927 2104 350-351 0.13042152850910682 2111 1 0.008881378387684435 2111 2 -0.21467748536087328 2111 3 -0.3031530325541816 2111 4 -0.2963354515751675 2111 5 -0.0178393532441099 2111 6 -0.005033629605641465 2111 7 -0.031785593977140536 2111 8 -0.041134812099919316 2111 9 -0.039314902785960726 2111 10-19 -0.10780274351009211 2111 20-29 -0.019899326390216743 2111 30-39 -0.20371722099131517 2111 40-49 -0.17173668918215412 2111 50-59 0.2216129911308684 2111 60-69 -0.4466355898404615 2111 70-79 -0.15590872280338886 2111 80-89 -0.0701801397916455 2111 90-99 0.04553237154352985 2111 100-109 -0.11683594176212608 2111 110-119 -0.10106318432394801 2111 120-129 -0.09099871060252696 2111 130-139 -0.07351209188784935 2111 140-149 -2.106263377489544 2111 150-159 0.18729362678698536 2111 160-169 -0.016842021154246822 2111 170-179 0.02882805298911606 2111 180-189 -0.06064735220836326 2111 190-199 0.2061913827301325 2111 200-209 -1.0546453027932898 2111 210-219 -0.42170525477909493 2111 220-229 -0.004432081466976712 2111 230-239 -0.24239908801181542 2111 240-249 -0.3154562217690611 2111 250-259 -0.19835291390478815 2111 260-269 -0.2543336758078176 2111 270-279 -0.28161970916690393 2111 280-289 -0.35531029245429124 2111 290-299 -0.26493741625753486 2111 300-309 -0.33886027470729374 2111 310-319 -0.2437022191429321 2111 320-329 -0.16486783272591055 2111 330-339 -0.10626696776183664 2111 340-349 -0.0897795787953477 2111 350-351 -0.03006156665113835 2110 1 0.2898062460657904 2110 2 0.12439854515998405 2110 3 0.07435176205212102 2110 4 0.1621401026114313 2110 5 0.06057839851766644 2110 6 -0.13182120078466397 2110 7 -0.007111765112348678 2110 8 0.002234335112142105 2110 9 -0.31201865062945444 2110 10-19 -0.08625545571717907 2110 20-29 -0.24062688488181294 2110 30-39 -0.1257434495563814 2110 40-49 -0.04646856884832573 2110 50-59 0.025251801643960903 2110 60-69 -0.3425381707762014 2110 70-79 -0.17424074710559267 2110 80-89 -0.28932990782670487 2110 90-99 -0.41621205648206683 2110 100-109 -0.41105133824981266 2110 110-119 -0.31639197496546956 2110 120-129 -0.28412153180217103 2110 130-139 -0.19468734913449026 2110 140-149 -0.06085761466230366 2110 150-159 -0.17340359077456569 2110 160-169 -0.19770955283561875 2110 170-179 -2.529741357213293 2110 180-189 -0.153265376924427 2110 190-199 -0.12091037758100498 2110 200-209 -1.545436655072269 2110 210-219 -0.8862962515041346 2110 220-229 -0.21058921372273431 2110 230-239 -0.17858219583511215 2110 240-249 -0.1357049983339813 2110 250-259 -0.12193661514590559 2110 260-269 -0.2031636344690071 2110 270-279 -0.05611633146225259 2110 280-289 -0.2692913297701267 2110 290-299 -0.21614442527086553 2110 300-309 -0.16754228035959784 2110 310-319 -0.08158194827962717 2110 320-329 -0.06332953703101651 2110 330-339 -0.04041878129341825 2110 340-349 -0.05619351508877912 2110 350-351 -0.14178387183401853 2109 1 0.12458179002638303 2109 2 0.12033159427281959 2109 3 0.39754568225437126 2109 4 0.02786250177067018 2109 5 -0.2637322614124926 2109 6 0.007659341310642276 2109 7 -0.013368358034206551 2109 8 0.013129386502235718 2109 9 -0.11266625565064459 2109 10-19 -0.08921330634073854 2109 20-29 -0.21306205632699715 2109 30-39 -0.11638901671162927 2109 40-49 -0.12483527434725517 2109 50-59 -0.1442162788707293 2109 60-69 -0.05131959651639306 2109 70-79 -0.20159741883348659 2109 80-89 -0.3420123223270224 2109 90-99 -0.309865296266743 2109 100-109 -0.5130713044727173 2109 110-119 -0.39956209327180403 2109 120-129 -0.5717529756751034 2109 130-139 -0.3361263094432445 2109 140-149 -0.0307310460982535 2109 150-159 -0.37464693216412215 2109 160-169 -0.3977130719876065 2109 170-179 -2.761640301758149 2109 180-189 -0.3560281746651164 2109 190-199 -0.05185313273117487 2109 200-209 -0.117991653304923 2109 210-219 -0.2872133046864924 2109 220-229 -0.13079885926577361 2109 230-239 -0.13431154076016583 2109 240-249 -0.2876311328873058 2109 250-259 -0.1948568216505535 2109 260-269 -0.05999124526267963 2109 270-279 -0.20782084049562854 2109 280-289 -0.20676069339058856 2109 290-299 -0.01391528902949446 2109 300-309 -0.10500809105501929 2109 310-319 -0.2504741712441785 2109 320-329 -0.08538173895630763 2109 330-339 -0.12031382545607627 2109 340-349 0.0098152187574847 2109 350-351 -0.48450104758553536 2108 1 0.13993592457306647 2108 2 -0.13956269939892962 2108 3 -0.1724305909099222 2108 4 -0.04379677249868763 2108 5 -0.4557178130310966 2108 6 0.13538289334895381 2108 7 -0.011939713823927889 2108 8 -1.436461779675824E-4 2108 9 -0.04668198317049388 2108 10-19 -0.10689218709154602 2108 20-29 -0.46132135297673216 2108 30-39 -0.17873462852070077 2108 40-49 -0.10637441483169852 2108 50-59 -0.17744359857528025 2108 60-69 -0.17778184161362987 2108 70-79 -0.18857818202243948 2108 80-89 -0.2724955519059904 2108 90-99 -0.44811679769266277 2108 100-109 -0.3254845501970962 2108 110-119 -0.21322816231980113 2108 120-129 -0.2124855826672345 2108 130-139 -0.09655538185255352 2108 140-149 0.18637712752978786 2108 150-159 0.005719869431736413 2108 160-169 -0.20232132142991688 2108 170-179 -0.09424290424994908 2108 180-189 0.04637564582850828 2108 190-199 0.1327054325837338 2108 200-209 0.2734341766794408 2108 210-219 -0.13135041995936803 2108 220-229 0.0799332503847161 2108 230-239 0.1477967450729949 2108 240-249 -0.008614333353591519 2108 250-259 -0.029864594130096123 2108 260-269 0.1295366274008778 2108 270-279 0.010124064966994695 2108 280-289 0.05435234508788156 2108 290-299 0.03464206386351876 2108 300-309 0.01827028046646717 2108 310-319 -0.03539425693250564 2108 320-329 -0.03518083112583881 2108 330-339 0.02495217196259958 2108 340-349 -0.031216581692063983 2108 350-351 0.26239538131947526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 13.0 17 149.0 18 2126.0 19 14174.0 20 51531.0 21 75071.0 22 36990.0 23 36444.0 24 48562.0 25 62792.0 26 72237.0 27 80835.0 28 86095.0 29 81552.0 30 79725.0 31 64731.0 32 45166.0 33 41184.0 34 46378.0 35 46699.0 36 36617.0 37 5466.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.04494652250923819 99.79389653823067 0.10862076273065896 0.0525361765294385 2 99.68330443679346 0.10625515628280433 0.1803774916489164 0.0300629152748194 3 0.3340433438241408 0.2096518714411176 99.26922474148357 0.18708004325117122 4 0.02316322980191003 0.03173855317538311 99.92577909769857 0.019319119324146237 5 0.040806711225492565 0.1938811617887533 0.11374624336767734 99.65156588361808 6 0.022473261254619094 0.04977630234027474 0.49825585807938383 99.42949457832573 7 0.8425501631775615 0.14509052880175136 98.78624675837992 0.2261125496407728 8 46.56321738247618 7.033342237213159 7.586105610528526 38.817334769782136 9 27.359519939598183 5.4407962631303475 14.586723625213027 52.61296017205844 10-19 19.219586432852754 20.20681314370369 28.352581813020493 32.22101861042306 20-29 23.8108342803973 24.15660708952539 21.079219231591885 30.95333939848542 30-39 23.112250982958763 20.717360298618388 24.182165495855752 31.988223222567097 40-49 23.208097470871007 23.137010997112974 25.645056523209064 28.009835008806956 50-59 20.217290808928983 18.91671981067263 30.5012720063004 30.36471737409799 60-69 23.321232599239654 21.76436785574532 29.194708138376146 25.71969140663888 70-79 25.0205857044431 18.172460595403432 29.82846396244994 26.978489737703526 80-89 16.965547899095057 37.058742936447 18.01191477114236 27.963794393315585 90-99 20.70540617023162 23.489064835266543 25.34761358090853 30.457915413593305 100-109 18.460896259727946 24.85114167673282 25.429838295934555 31.25812376760468 110-119 18.533587583557686 24.663132580662776 26.2183814396022 30.584898396177334 120-129 17.17208873948758 28.886265692114893 24.60789549373301 29.33375007466451 130-139 18.484731578420295 26.45289719935687 25.99882862984782 29.063542592375015 140-149 22.022558250411343 26.41082210925782 25.15078046862517 26.415839171705663 150-159 20.603015422095122 26.109739222162133 23.842992952752184 29.44425240299056 160-169 21.35088089356929 25.126845914100805 27.868468209175013 25.65380498315489 170-179 17.919560059979993 26.40249264123395 26.641980889237992 29.03596640954807 180-189 17.534623398495018 28.639896055139978 25.365843897995333 28.459636648369667 190-199 16.41899733297376 29.20703232268632 23.92874906040995 30.445221283929968 200-209 16.502849917612757 27.90495497409117 26.028849976161567 29.563345132134515 210-219 15.009991738112966 28.17186183822354 25.972250231681766 30.84589619198173 220-229 14.29712976517766 29.02634817136326 27.239080045561657 29.437442017897418 230-239 15.018526161111906 31.11361098935348 26.174753580027488 27.693109269507126 240-249 14.085796465249267 31.850537333090333 25.832154389834272 28.231511811826127 250-259 14.085489519174436 30.84710248522875 26.715735944543844 28.351672051052972 260-269 13.043503545456158 30.84515316331177 25.76749219841938 30.34385109281269 270-279 13.96299458837475 30.41516134288106 26.839080760002997 28.78276330874119 280-289 13.128898140168785 29.93643512521903 27.054897779610688 29.879768955001502 290-299 12.795758859816559 30.931334789167686 26.29389619023822 29.979010160777538 300-309 12.145987982908087 32.88499803309545 26.004695929172904 28.964318054823558 310-319 11.50014400634929 34.06779199923985 23.955569556446648 30.476494437964213 320-329 10.240371384661241 35.32935766650134 23.485981407899395 30.944289540938026 330-339 9.757522214784794 35.91932409057401 23.01238898521623 31.310764709424966 340-349 8.106225212875053 41.664192636102236 20.899713249015218 29.329868902007494 350-351 5.928949010338687 42.42163189389467 20.120567075292325 31.528852020474325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.75 4 0.25 5 0.0 6 0.0 7 0.25 8 0.5 9 0.75 10 0.25 11 0.25 12 0.75 13 0.5 14 0.5 15 0.0 16 1.0 17 1.5 18 0.5 19 0.25 20 0.75 21 1.25 22 0.25 23 1.5 24 1.0 25 2.75 26 3.25 27 9.0 28 12.25 29 24.0 30 41.75 31 66.25 32 118.25 33 237.5 34 453.75 35 796.5 36 1296.5 37 1963.75 38 2945.25 39 4185.25 40 5710.75 41 7740.5 42 10446.75 43 14124.0 44 19184.5 45 24930.0 46 30269.5 47 33122.75 48 32612.0 49 31509.0 50 34778.5 51 25443.75 52 20491.5 53 16554.25 54 9432.75 55 2322.25 56 1808.5 57 2467.75 58 1450.0 59 419.25 60 238.75 61 203.0 62 151.0 63 112.75 64 71.25 65 47.25 66 33.25 67 19.75 68 14.0 69 10.5 70 11.25 71 7.0 72 6.75 73 7.5 74 5.0 75 3.0 76 2.0 77 1.0 78 0.75 79 0.5 80 1.25 81 0.5 82 1.5 83 0.5 84 0.75 85 1.25 86 1.0 87 1.25 88 0.0 89 0.75 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-19 0.0 20-29 0.0 30-39 0.0 40-49 0.0 50-59 0.0 60-69 0.0 70-79 0.0 80-89 0.0 90-99 9.856693532727673E-6 100-109 4.928346766363837E-5 110-119 3.942677413091069E-5 120-129 7.885354826182139E-5 130-139 5.9140161196366035E-5 140-149 1.084236288600044E-4 150-159 1.084236288600044E-4 160-169 9.856693532727673E-5 170-179 6.899685472909371E-5 180-189 1.084236288600044E-4 190-199 7.885354826182139E-5 200-209 8.871024179454905E-5 210-219 7.885354826182139E-5 220-229 1.774204835890981E-4 230-239 6.899685472909371E-5 240-249 6.899685472909371E-5 250-259 5.9140161196366035E-5 260-269 9.856693532727673E-5 270-279 8.871024179454905E-5 280-289 6.899685472909371E-5 290-299 4.928346766363837E-5 300-309 6.899685472909371E-5 310-319 3.942677413091069E-5 320-329 5.9140161196366035E-5 330-339 6.899685472909371E-5 340-349 1.9713387065455346E-5 350-351 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 351 1014539.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.578442448281383 #Duplication Level Percentage of deduplicated Percentage of total 1 75.413561779955 4.961037759893466 2 8.432566487960903 1.1094630666471412 3 3.778701850106378 0.7457391795041757 4 2.19836328185404 0.5784722532036716 5 1.5050684547878486 0.49505031052728254 6 1.009024054097244 0.39826840012861675 7 0.8584117404312133 0.39529085619490556 8 0.7045023541525715 0.37076225531771523 9 0.5017822106904631 0.29708508551387536 >10 3.835167099885367 4.957273643232407 >50 0.6314315150320229 3.006391257047804 >100 0.7140362835032852 9.853601815769395 >500 0.1345908586644577 6.348802636275559 >1k 0.2540591489396489 34.57340764341521 >5k 0.01512256838926482 6.628946611701774 >10k+ 0.013610311550338336 25.280407225627027 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGTTCCTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA 66077 6.513007385620464 No Hit AGTTCCTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 42073 4.147006670024513 No Hit AGTTCCTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT 40347 3.976880139649634 No Hit AGTTCCTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC 22488 2.2165732416397987 No Hit AGTTCCTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT 20996 2.069511374131502 No Hit AGTTCCTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC 19552 1.9271807195189146 No Hit AGTTCCTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA 18096 1.7836672616823996 No Hit AGTTCCTGCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGG 13612 1.3416931236748908 No Hit AGTTCCTCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCC 10877 1.0721125555547888 No Hit AGTTCCTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT 9299 0.9165739316083462 No Hit AGTTCCTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG 9046 0.8916364969705453 No Hit AGTTCCTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 8155 0.8038133575939417 No Hit AGTTCCTGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTA 6012 0.5925844151875876 No Hit AGTTCCTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT 5944 0.5858818635853328 No Hit AGTTCCTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG 5791 0.5708011224802595 No Hit AGTTCCTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT 5754 0.5671541458731503 No Hit AGTTCCTGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTC 5749 0.5666613111965139 No Hit AGTTCCTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA 5728 0.564591405554641 No Hit AGTTCCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACT 5156 0.5082111185474388 No Hit AGTTCCTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC 4894 0.48238658149169233 No Hit AGTTCCTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC 4826 0.4756840298894375 No Hit AGTTCCTACCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 4591 0.4525208000875274 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGG 4569 0.45035232751032733 TruSeq Adapter, Index 19 (97% over 36bp) AGTTCCTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA 4275 0.421373648524108 No Hit AGTTCCTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC 4259 0.4197965775588715 No Hit AGTTCCTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT 4178 0.4118126557973622 No Hit AGTTCCTCCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCA 4135 0.40757427757828923 No Hit AGTTCCTGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACCGC 4105 0.404617269518471 No Hit AGTTCCTCCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 4085 0.40264593081192546 No Hit AGTTCCTCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCC 4069 0.401068859846689 No Hit AGTTCCTCCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCC 4061 0.40028032436407074 No Hit AGTTCCTCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC 3775 0.37209018086046963 No Hit AGTTCCTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC 3726 0.3672604010294331 No Hit AGTTCCTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT 3667 0.3614449518451237 No Hit AGTTCCTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA 3665 0.3612478179744692 No Hit AGTTCCTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA 3658 0.3605578494271783 No Hit AGTTCCTCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCC 3580 0.3528696284716507 No Hit AGTTCCTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCT 3554 0.3503068881531415 No Hit AGTTCCTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCG 3494 0.3443928720335049 No Hit AGTTCCTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG 3423 0.33739461962526823 No Hit AGTTCCTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC 3385 0.33364907608283173 No Hit AGTTCCTGTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTAC 3249 0.32024397287832207 No Hit AGTTCCTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT 3229 0.31827263417177654 No Hit AGTTCCTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA 3226 0.3179769333657947 No Hit AGTTCCTGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC 3140 0.3095001769276489 No Hit AGTTCCTCCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGT 3111 0.3066417358031579 No Hit AGTTCCTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC 2867 0.28259140358330237 No Hit AGTTCCTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC 2848 0.2807186318120841 No Hit AGTTCCTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTT 2835 0.2794372616528295 No Hit AGTTCCTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCG 2800 0.27598741891637485 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGT 2725 0.26859489876682907 TruSeq Adapter, Index 9 (97% over 36bp) AGTTCCTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC 2677 0.2638636858711198 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC 2657 0.26189234716457427 TruSeq Adapter, Index 6 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGG 2653 0.2614980794232652 TruSeq Adapter, Index 10 (97% over 36bp) AGTTCCTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA 2475 0.24395316493500988 No Hit AGTTCCTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGC 2418 0.23833484962135512 No Hit AGTTCCTCGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT 2416 0.23813771575070058 No Hit AGTTCCTGAGGGATGTCCACCTGCGGGAAGCGTCAACTCGAGGAAACGAA 2410 0.23754631413873692 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTG 2399 0.23646207785013684 TruSeq Adapter, Index 10 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGG 2366 0.23320936898433672 TruSeq Adapter, Index 7 (97% over 36bp) AGTTCCTACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTA 2340 0.23064662866582752 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGT 2325 0.22916812463591837 TruSeq Adapter, Index 13 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTT 2322 0.22887242382993658 TruSeq Adapter, Index 12 (97% over 36bp) AGTTCCTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC 2317 0.2283795891533002 No Hit AGTTCCTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCA 2308 0.2274924867353547 No Hit AGTTCCTCCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCT 2297 0.22640825044675464 No Hit AGTTCCTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG 2295 0.22621111657610007 No Hit AGTTCCTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA 2290 0.2257182818994637 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT 2268 0.2235498093222636 TruSeq Adapter, Index 8 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGG 2263 0.2230569746456272 TruSeq Adapter, Index 15 (97% over 36bp) AGTTCCTCGCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGT 2230 0.2198042657798271 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG 2206 0.21743865933197246 TruSeq Adapter, Index 15 (97% over 36bp) AGTTCCTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT 2176 0.21448165127215413 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGA 2164 0.21329884804822682 TruSeq Adapter, Index 2 (97% over 36bp) AGTTCCTGCCGGTGCTTATTCTGTCGGTAACGTCAAAACAATCACGTATT 2164 0.21329884804822682 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAT 2161 0.21300314724224498 TruSeq Adapter, Index 9 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT 2151 0.21201747788897224 TruSeq Adapter, Index 12 (100% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGC 2101 0.2070891311226084 TruSeq Adapter, Index 11 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA 2095 0.20649772951064474 TruSeq Adapter, Index 3 (100% over 36bp) AGTTCCTACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCC 2087 0.2057091940280265 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCG 2055 0.20255505209755367 TruSeq Adapter, Index 16 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG 2040 0.20107654806764452 TruSeq Adapter, Index 15 (100% over 36bp) AGTTCCTTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTAT 1997 0.19683816984857164 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAT 1960 0.19319119324146236 TruSeq Adapter, Index 9 (97% over 36bp) AGTTCCTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG 1956 0.1927969255001533 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCA 1952 0.19240265775884416 TruSeq Adapter, Index 5 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA 1938 0.1910227206642623 TruSeq Adapter, Index 2 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCT 1919 0.189149948893044 TruSeq Adapter, Index 8 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAT 1916 0.1888542480870622 TruSeq Adapter, Index 8 (97% over 36bp) AGTTCCTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC 1900 0.18727717712182576 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATA 1896 0.1868829093805167 TruSeq Adapter, Index 3 (97% over 36bp) AGTTCCTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT 1885 0.1857986730919166 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT 1882 0.1855029722859348 TruSeq Adapter, Index 9 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG 1877 0.1850101376092984 TruSeq Adapter, Index 7 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC 1870 0.1843201690620075 TruSeq Adapter, Index 6 (100% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTA 1869 0.1842216021266802 TruSeq Adapter, Index 3 (97% over 36bp) AGTTCCTGTCAGCATTCGCACTTCTGATACCTCCAGCAAGCTTCTCAACT 1868 0.18412303519135292 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCT 1854 0.18274309809677103 TruSeq Adapter, Index 8 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA 1849 0.18225026342013464 TruSeq Adapter, Index 3 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG 1826 0.17998322390760732 TruSeq Adapter, Index 7 (97% over 36bp) AGTTCCTACGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCC 1811 0.17850471987769814 No Hit AGTTCCTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTTTCCGAGCGT 1781 0.17554771181787984 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAG 1779 0.17535057794722528 TruSeq Adapter, Index 7 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC 1773 0.17475917633526164 TruSeq Adapter, Index 1 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC 1769 0.1743649085939525 TruSeq Adapter, Index 1 (100% over 36bp) AGTTCCTTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCT 1764 0.17387207391731616 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT 1758 0.17328067230535246 TruSeq Adapter, Index 8 (97% over 36bp) AGTTCCTGCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGAC 1743 0.1718021682754433 No Hit AGTTCCTTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 1742 0.17170360134011606 No Hit AGTTCCTGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTAC 1739 0.17140790053413424 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC 1727 0.1702250973102069 TruSeq Adapter, Index 11 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCA 1726 0.17012653037487963 TruSeq Adapter, Index 3 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAA 1721 0.16963369569824324 TruSeq Adapter, Index 5 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT 1705 0.1680566247330068 TruSeq Adapter, Index 9 (100% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG 1704 0.16795805779767953 TruSeq Adapter, Index 7 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT 1698 0.1673666561857159 TruSeq Adapter, Index 12 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG 1695 0.16707095537973404 TruSeq Adapter, Index 10 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG 1693 0.16687382150907948 TruSeq Adapter, Index 7 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCT 1690 0.16657812070309766 TruSeq Adapter, Index 8 (97% over 36bp) AGTTCCTCTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAG 1681 0.16569101828515218 No Hit AGTTCCTGAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAG 1676 0.1651981836085158 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTC 1668 0.16440964812589756 TruSeq Adapter, Index 1 (97% over 36bp) AGTTCCTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT 1645 0.1621426086133702 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTC 1610 0.15869276587691553 TruSeq Adapter, Index 1 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTA 1607 0.1583970650709337 TruSeq Adapter, Index 2 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC 1585 0.1562285924937336 TruSeq Adapter, Index 6 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAA 1577 0.15544005701111538 TruSeq Adapter, Index 7 (97% over 35bp) AGTTCCTGGCCAGATTTTCTTAAAGATCTTCTTCAAGACGGAGATGACCT 1572 0.154947222334479 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAC 1568 0.1545529545931699 TruSeq Adapter, Index 7 (97% over 35bp) AGTTCCTTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC 1557 0.15346871830456987 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG 1550 0.15277874975727893 TruSeq Adapter, Index 7 (100% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA 1537 0.15149737959802434 TruSeq Adapter, Index 2 (97% over 36bp) AGTTCCTTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACT 1535 0.15130024572736978 No Hit AGTTCCTCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTG 1532 0.15100454492138793 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG 1523 0.15011744250344244 TruSeq Adapter, Index 15 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGC 1515 0.14932890702082424 TruSeq Adapter, Index 1 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA 1505 0.14834323766755145 TruSeq Adapter, Index 2 (100% over 36bp) AGTTCCTCACCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTC 1490 0.14686473363764233 No Hit AGTTCCTCACCCGTCCGCCGCTCGCCACCAGGTACAAGTACCCGTGCTGC 1487 0.1465690328316605 No Hit AGTTCCTGTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATG 1467 0.14459769412511495 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT 1455 0.14341489090118764 TruSeq Adapter, Index 8 (100% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCA 1449 0.14282348928922398 TruSeq Adapter, Index 2 (97% over 36bp) AGTTCCTTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGT 1429 0.14085215058267844 No Hit AGTTCCTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC 1422 0.1401621820353875 No Hit AGTTCCTGACCCATTATACAAAAGGTACGCAGTCACCCAACAAAGTGGGC 1416 0.13957078042342383 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGA 1408 0.13878224494080563 TruSeq Adapter, Index 2 (97% over 36bp) AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC 1398 0.13779657558753286 TruSeq Adapter, Index 7 (97% over 35bp) AGTTCCTCCTCTACCATACTCTAGCTTGCCAGTTTTGGATGCAGTTCCCA 1390 0.13700804010491466 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA 1387 0.1367123392989328 TruSeq Adapter, Index 5 (100% over 36bp) AGTTCCTACTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAA 1386 0.13661377236360556 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAC 1378 0.13582523688098733 TruSeq Adapter, Index 1 (97% over 36bp) AGTTCCTCGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT 1375 0.13552953607500548 No Hit AGTTCCTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTA 1357 0.1337553312391145 No Hit AGTTCCTCGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAA 1356 0.13365676430378723 No Hit AGTTCCTGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC 1346 0.13267109495051446 No Hit AGTTCCTGCCTCTGACTGCCAAGGCATCCACCGTATGCGCTAGGAACTAG 1345 0.1325725280151872 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCC 1318 0.12991122076135073 TruSeq Adapter, Index 6 (97% over 36bp) AGTTCCTTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTC 1294 0.1275456143134961 No Hit AGTTCCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACC 1287 0.12685564576620514 No Hit AGTTCCTCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGA 1277 0.12586997641293238 No Hit AGTTCCTTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGT 1261 0.12429290544769594 No Hit AGTTCCTGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTAC 1252 0.12340580302975046 No Hit AGTTCCTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT 1237 0.12192729899984131 No Hit AGTTCCTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC 1236 0.12182873206451404 No Hit AGTTCCTTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA 1236 0.12182873206451404 No Hit AGTTCCTACCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA 1235 0.12173016512918675 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACC 1223 0.12054736190525944 TruSeq Adapter, Index 1 (97% over 36bp) AGTTCCTCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTC 1205 0.11877315706936846 No Hit AGTTCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAGTATTTAGCCT 1201 0.11837888932805934 No Hit AGTTCCTTCCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGC 1191 0.11739321997478658 No Hit AGTTCCTGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAC 1169 0.1152247473975865 TruSeq Adapter, Index 6 (97% over 36bp) AGTTCCTCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTC 1154 0.11374624336767734 No Hit AGTTCCTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC 1146 0.11295770788505913 No Hit AGTTCCTCCGTCTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAAT 1138 0.11216917240244093 No Hit AGTTCCTCCTTCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCA 1128 0.11118350304916814 No Hit AGTTCCTGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACT 1127 0.11108493611384086 No Hit AGTTCCTGTCCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACT 1123 0.11069066837253176 No Hit AGTTCCTCCCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTCCGTT 1104 0.10881789660131351 No Hit AGTTCCTTAGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCAC 1095 0.10793079418336801 No Hit AGTTCCTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCTGTATTACC 1083 0.10674799095944068 No Hit AGTTCCTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTC 1074 0.1058608885414952 No Hit AGTTCCTGGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC 1054 0.10388954983494966 No Hit AGTTCCTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT 1042 0.10270674661102236 No Hit AGTTCCTGCGGCATGGCTGGATCAGGCTTTCGCCCATTGTCCAATATTCC 1037 0.10221391193438598 No Hit AGTTCCTGCCCGGGGATTTCACATCCAACTTAACAAACCACCTACGCGCG 1030 0.10152394338709503 No Hit AGTTCCTCGCCTCTGACTGCCAAGGCATCCACCGTATGCGCTTCAGGAAC 1027 0.1012282425811132 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 2.957008059818302E-4 0.0 0.0 8 0.007096819343563924 0.0 0.0 9 0.007589654020200308 0.0 0.0 10-19 8.643226135220036 0.0 0.0 20-29 14.277893703445605 0.0 0.0 30-39 14.289672452217214 0.0 0.0 40-49 14.564615061619122 0.0 0.0 50-59 16.218252822217774 0.0 0.0 60-69 17.911691911301585 0.0 0.0 70-79 20.22450590859494 0.0 0.0 80-89 23.071119000846686 0.0 0.0 90-99 26.218390815927233 0.0 0.0 100-109 29.620744002941233 0.0 9.856693532727671E-6 110-119 34.02787867198797 0.0 9.856693532727672E-5 120-129 38.5679801367912 6.89968547290937E-5 9.856693532727672E-5 130-139 43.57796003899308 9.856693532727672E-5 1.4785040299091504E-4 140-149 47.98620851440901 9.856693532727672E-5 1.9713387065455344E-4 150-159 52.741826583305325 9.856693532727672E-5 1.9713387065455344E-4 160-169 57.49429051027117 9.856693532727672E-5 1.9713387065455344E-4 170-179 62.66406712802565 9.856693532727672E-5 1.9713387065455344E-4 180-189 66.19356180491829 9.856693532727672E-5 2.7598741891637484E-4 190-199 68.59213889263992 9.856693532727672E-5 2.957008059818302E-4 200-209 70.48978895833477 9.856693532727672E-5 4.041244348418345E-4 210-219 72.0646323108328 9.856693532727672E-5 4.928346766363835E-4 220-229 73.53454130398141 9.856693532727672E-5 5.519748378327497E-4 230-239 75.66655397180394 9.856693532727672E-5 5.914016119636604E-4 240-249 77.54748708526729 9.856693532727672E-5 5.914016119636604E-4 250-259 78.86951610534439 9.856693532727672E-5 6.998252408236647E-4 260-269 79.9642793426374 9.856693532727672E-5 9.166724985436734E-4 270-279 80.93907676294356 9.856693532727672E-5 0.0010546662080018609 280-289 81.82392199807006 9.856693532727672E-5 0.0011630898368618654 290-299 83.01669033915897 9.856693532727672E-5 0.0011828032239273208 300-309 84.23146867690646 9.856693532727672E-5 0.0013602237075164187 310-319 85.1326168831361 9.856693532727672E-5 0.0014883607234418784 320-329 86.02853118509982 9.856693532727672E-5 0.0015770709652364275 330-339 86.68817068639056 9.856693532727672E-5 0.0016953512876291595 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGGAA 14945 0.0 339.1131 5 GTTCCTC 39610 0.0 338.16238 2 TCCTGGA 15115 0.0 337.69568 4 TTCCTGC 21995 0.0 334.2552 3 CTGGAAC 15245 0.0 333.4582 6 TGGAACT 15340 0.0 332.63007 7 AGTTCCT 103520 0.0 328.6195 1 TCCTGCC 16395 0.0 327.6392 4 GTTCCTA 7485 0.0 320.57083 2 TCCTGGC 2005 0.0 311.44608 4 TTCCTCG 6720 0.0 308.8055 3 TTCCTGT 6675 0.0 307.26935 3 CCTGCCC 8305 0.0 306.78177 5 TCCTGGG 610 0.0 305.40952 4 CCTGGCC 1530 0.0 304.41144 5 TCCTCGG 1990 0.0 302.52484 4 CCTGCCT 7040 0.0 301.13962 5 CTGCCCC 6235 0.0 300.73346 6 GTTCCTG 52545 0.0 299.99112 2 TCCTGCG 4060 0.0 299.96277 4 >>END_MODULE