##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AGWYV l01n01 ca65a.3410000000eafb.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 409508 Sequences flagged as poor quality 0 Sequence length 351 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.673112613184603 32.0 32.0 33.0 30.0 34.0 2 31.774375592174025 32.0 32.0 33.0 29.0 34.0 3 31.510375865672955 33.0 32.0 33.0 29.0 34.0 4 31.44533440128154 33.0 32.0 33.0 29.0 34.0 5 32.065158678218744 33.0 32.0 34.0 31.0 34.0 6 35.92318587182668 37.0 37.0 37.0 34.0 37.0 7 36.73190755736152 37.0 37.0 37.0 37.0 37.0 8 35.13577756722702 37.0 33.0 37.0 32.0 37.0 9 34.79185510417379 37.0 33.0 37.0 32.0 37.0 10-19 36.017557166160366 37.1 35.9 37.7 33.6 37.7 20-29 36.88785444972992 38.0 37.1 38.7 34.3 38.7 30-39 36.93531042128603 38.0 37.0 39.0 34.0 39.0 40-49 37.01509494320013 38.0 37.0 39.0 34.5 39.0 50-59 37.04740420211571 38.2 37.4 39.0 34.9 39.0 60-69 36.837697920431346 38.0 37.0 39.0 34.1 39.0 70-79 36.776459312150195 38.0 37.0 39.0 33.8 39.0 80-89 36.83276443927836 38.0 37.0 39.0 33.9 39.0 90-99 36.802633403987215 38.0 37.0 39.0 33.9 39.0 100-109 36.807356632837454 38.0 37.0 39.0 34.0 39.0 110-119 36.58979507115856 38.0 37.0 39.0 33.5 39.0 120-129 36.439126219756396 38.0 37.0 39.0 32.5 39.0 130-139 36.11778890766481 38.0 37.0 39.0 31.3 39.0 140-149 35.23771183957334 38.0 35.5 39.0 26.4 39.0 150-159 35.00261753128144 38.0 35.5 38.9 24.9 39.0 160-169 34.31627709348779 38.0 34.4 38.4 17.3 39.0 170-179 33.128295662111604 37.2 32.4 38.0 14.9 39.0 180-189 32.42805219922443 37.0 31.2 38.0 14.3 38.6 190-199 31.333075055920766 37.0 25.9 37.9 14.0 38.0 200-209 30.06043227482735 36.5 22.4 37.0 13.8 37.9 210-219 29.214635367318827 35.9 15.1 37.0 13.6 37.0 220-229 28.39369731482658 33.9 14.4 37.0 13.1 37.0 230-239 27.423578782343686 32.8 14.0 37.0 13.0 37.0 240-249 26.35987135782451 29.6 14.0 37.0 13.0 37.0 250-259 25.38419151762603 25.8 14.0 37.0 13.0 37.0 260-269 24.391316408959042 24.2 14.0 37.0 13.0 37.0 270-279 23.502676138195103 24.0 14.0 34.6 13.0 37.0 280-289 22.95626630004786 20.5 14.0 33.1 13.0 37.0 290-299 22.112655674614416 15.0 14.0 32.7 13.0 37.0 300-309 21.158055520282876 14.7 14.0 29.7 13.0 37.0 310-319 20.179511999765573 14.0 14.0 26.0 13.0 35.7 320-329 19.53621492132022 14.0 14.0 25.3 13.0 33.7 330-339 18.585389540619474 14.0 14.0 24.2 13.0 31.8 340-349 18.21722164157965 14.0 13.7 24.0 13.0 26.1 350-351 18.010143879973043 14.0 13.5 24.0 13.0 26.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11078226435971317 1101 2 0.8056545927947063 1101 3 0.7760508975890694 1101 4 0.530897112176028 1101 5 0.4771779137685783 1101 6 0.3093614966878775 1101 7 0.05372412569288798 1101 8 0.054078317950931876 1101 9 0.14094425348280026 1101 10-19 0.12644860768966737 1101 20-29 0.14371389582253613 1101 30-39 0.11767190639333336 1101 40-49 0.14884653148896376 1101 50-59 0.10637590596267188 1101 60-69 0.2544248888031575 1101 70-79 0.20297307143442112 1101 80-89 0.08218247788673949 1101 90-99 0.3131319343080463 1101 100-109 0.314695498886266 1101 110-119 0.2471361700429 1101 120-129 0.26683208493563626 1101 130-139 0.15848455712795584 1101 140-149 0.3883879002273929 1101 150-159 0.19364235904613736 1101 160-169 0.20075667275304454 1101 170-179 0.3805924215933416 1101 180-189 -0.20621560937765793 1101 190-199 0.04086004684555178 1101 200-209 -0.08008831469047095 1101 210-219 -0.017462293772975812 1101 220-229 0.01705644515650917 1101 230-239 0.08501988594101206 1101 240-249 0.19237055756672206 1101 250-259 -0.07060121583884893 1101 260-269 0.041821914781035474 1101 270-279 0.0843011391982138 1101 280-289 0.047707855707312063 1101 290-299 -0.008460580173821342 1101 300-309 -0.0516013263758488 1101 310-319 0.17875146347932613 1101 320-329 0.11352084170007615 1101 330-339 0.09954304301481542 1101 340-349 0.146210958133846 1101 350-351 0.1368115937839285 1102 1 -0.5870316358592973 1102 2 0.2472377067813234 1102 3 -1.0033569667441533 1102 4 -0.10544981060723302 1102 5 -0.08206076894556347 1102 6 0.008992793278125077 1102 7 0.03505688303179966 1102 8 0.039225698850692936 1102 9 0.3034912204702849 1102 10-19 0.18201512499963002 1102 20-29 0.2857724061402038 1102 30-39 0.5018877837467031 1102 40-49 0.3550141257745665 1102 50-59 0.30458694094429006 1102 60-69 0.38963164401221206 1102 70-79 0.4924628140452896 1102 80-89 0.3253433812603177 1102 90-99 0.3105030890429177 1102 100-109 0.1668413125453725 1102 110-119 -0.6215094728271495 1102 120-129 0.09764152454663844 1102 130-139 0.18189933339430553 1102 140-149 0.854316590777735 1102 150-159 0.4363944738163852 1102 160-169 0.041877048890796686 1102 170-179 0.3217700622933748 1102 180-189 -0.04721804060995538 1102 190-199 0.07195601019623155 1102 200-209 0.15592416295626066 1102 210-219 -0.44441672323709724 1102 220-229 -0.13334068960610068 1102 230-239 -0.017639500423996424 1102 240-249 0.1574680077867363 1102 250-259 -0.14206799234631262 1102 260-269 -0.05617824596160048 1102 270-279 0.03015026214623262 1102 280-289 0.29408963954865897 1102 290-299 0.12250809510597094 1102 300-309 0.12964452947820249 1102 310-319 0.6105205365971287 1102 320-329 -0.06806237275068838 1102 330-339 0.1052416706746051 1102 340-349 0.15171448442531243 1102 350-351 -0.27788379893040727 1103 1 0.20170306992651987 1103 2 0.003617767117365389 1103 3 -0.25662094807199054 1103 4 0.2582222392986573 1103 5 0.329216580320697 1103 6 0.29451872784856903 1103 7 0.1354577388391931 1103 8 0.0696226646244753 1103 9 0.129392968756747 1103 10-19 0.1174955745416355 1103 20-29 -0.12882798407879648 1103 30-39 -0.026940303106400165 1103 40-49 -0.24455994593576236 1103 50-59 -0.9552238960410264 1103 60-69 0.07881537516226444 1103 70-79 -0.08733783186238497 1103 80-89 -0.6103760926126256 1103 90-99 0.09894406690010982 1103 100-109 -0.08833552189924632 1103 110-119 -0.17676370273218112 1103 120-129 0.019797038770583697 1103 130-139 0.06702613430765325 1103 140-149 0.7232889634348538 1103 150-159 -0.04962278010353316 1103 160-169 -0.1752435563335979 1103 170-179 0.23639780431120982 1103 180-189 -0.03492016533821385 1103 190-199 -0.07704863758351621 1103 200-209 0.12115501213843771 1103 210-219 0.2707081630503261 1103 220-229 0.22383049618555617 1103 230-239 0.07844620390508794 1103 240-249 0.2084031860495621 1103 250-259 0.27245692716849135 1103 260-269 0.21513569393333043 1103 270-279 0.3292274428443669 1103 280-289 0.1804600862339285 1103 290-299 0.2699526266709569 1103 300-309 0.4401051236403113 1103 310-319 0.5071125531135614 1103 320-329 0.2107718429314147 1103 330-339 0.24310137215504923 1103 340-349 0.0747990480830758 1103 350-351 -0.027867337199626974 2112 1 -0.01967237274901734 2112 2 0.6443894846805058 2112 3 0.8748272243552897 2112 4 0.6077125569486306 2112 5 0.2643338915803568 2112 6 0.23231989970534528 2112 7 0.044192126341300764 2112 8 0.0876310821872579 2112 9 0.13294154914518685 2112 10-19 0.01149061585803679 2112 20-29 3.607939393859283E-4 2112 30-39 -0.07192797815832819 2112 40-49 0.012327852186238886 2112 50-59 -0.07940232253805135 2112 60-69 -0.08922766707265595 2112 70-79 -0.8593209106236444 2112 80-89 -0.851639731683612 2112 90-99 -0.2309777495137766 2112 100-109 -0.1898887882795819 2112 110-119 0.06355197381237332 2112 120-129 -0.33231434862029374 2112 130-139 -0.0858581188220171 2112 140-149 0.46383176347647037 2112 150-159 -0.740597401833476 2112 160-169 -0.10753599667332026 2112 170-179 0.49864865916386947 2112 180-189 -0.11487660642423947 2112 190-199 0.04748931141595847 2112 200-209 0.3979582758277438 2112 210-219 0.19736664896543843 2112 220-229 0.28338242063559704 2112 230-239 0.49233231205605676 2112 240-249 0.47584594769905664 2112 250-259 0.27396247249810557 2112 260-269 -0.9301081678119658 2112 270-279 0.3605862728533751 2112 280-289 0.2374034991143077 2112 290-299 0.41361057079068786 2112 300-309 0.39950096796204804 2112 310-319 0.07649357879322949 2112 320-329 0.46099196973306533 2112 330-339 0.4670580572284031 2112 340-349 0.18551870305629592 2112 350-351 0.5101157487377286 1113 1 -0.22220725042950207 1113 2 -0.5155657873632009 1113 3 -0.6246819127385415 1113 4 -0.07575790983337782 1113 5 0.08819114952770946 1113 6 -0.10678492483867785 1113 7 0.04784608340069951 1113 8 0.08717323696535573 1113 9 0.18600757130665357 1113 10-19 -0.121305629811161 1113 20-29 0.18822745532322216 1113 30-39 0.13841360079135256 1113 40-49 0.054877962025067006 1113 50-59 0.11667605704205641 1113 60-69 0.2641522432072918 1113 70-79 0.3113844791831397 1113 80-89 -0.17454431938078585 1113 90-99 0.023731232617052456 1113 100-109 0.052756412489934235 1113 110-119 0.13524657343993596 1113 120-129 0.28594443078194587 1113 130-139 0.2643322831572803 1113 140-149 0.4620514499405459 1113 150-159 -1.467205693234348 1113 160-169 0.21321715871670932 1113 170-179 0.6612788038693651 1113 180-189 0.4335739837118382 1113 190-199 0.7289166438952392 1113 200-209 0.9848322055044818 1113 210-219 0.7385964916691918 1113 220-229 0.47859529665198863 1113 230-239 0.3074117085808332 1113 240-249 0.6877032584747056 1113 250-259 0.8496812674846979 1113 260-269 0.22658979961938286 1113 270-279 1.0192181961114741 1113 280-289 0.994283724162841 1113 290-299 0.8323831053602042 1113 300-309 0.7435232262836031 1113 310-319 0.5320231604371557 1113 320-329 0.47531768258486906 1113 330-339 0.5032780166386637 1113 340-349 0.41540587062324263 1113 350-351 0.41262333179922095 2113 1 0.22949491784976672 2113 2 0.2798871425353333 2113 3 0.6287144014304253 2113 4 -0.028876182196551525 2113 5 0.14288150559995216 2113 6 -0.03148819797362279 2113 7 0.04096967694379572 2113 8 0.19844022615239254 2113 9 0.18194661739376272 2113 10-19 -0.14555365772500295 2113 20-29 0.1041937369151853 2113 30-39 -0.030355541239480033 2113 40-49 0.025392430760092566 2113 50-59 0.058626299947832194 2113 60-69 0.050215321530174606 2113 70-79 0.13350175027085243 2113 80-89 0.09736157697399506 2113 90-99 -0.02656518960029075 2113 100-109 -0.15193551759544022 2113 110-119 0.05139777618907715 2113 120-129 0.11369448561853801 2113 130-139 0.2287455722963827 2113 140-149 0.5184353426070629 2113 150-159 -0.04555514243130432 2113 160-169 0.2539841499347375 2113 170-179 0.5770486892405984 2113 180-189 0.4243170093103714 2113 190-199 0.39514192681140514 2113 200-209 0.3889414968532918 2113 210-219 0.2711957763563859 2113 220-229 0.2013124698462434 2113 230-239 -0.12237176814002027 2113 240-249 0.2165939143202884 2113 250-259 0.39592572289645744 2113 260-269 0.11062108084577815 2113 270-279 0.35304145372969487 2113 280-289 0.3102928087574135 2113 290-299 0.37722084999230887 2113 300-309 0.13720046625662619 2113 310-319 0.5452239009341824 2113 320-329 0.32349588930661355 2113 330-339 0.25453737947814403 2113 340-349 0.14953717784623777 2113 350-351 -0.17929624323754112 1112 1 0.10701171329835191 1112 2 0.18828595962465755 1112 3 0.48049204583093186 1112 4 0.49333743775220285 1112 5 0.2735598467005502 1112 6 0.17708807252892456 1112 7 0.06494354060308183 1112 8 0.126008383057858 1112 9 0.2108695903394704 1112 10-19 0.17117361714200996 1112 20-29 0.06678754933070508 1112 30-39 0.15464993955756512 1112 40-49 0.19663850509718372 1112 50-59 0.2461971215966443 1112 60-69 0.06951403169352943 1112 70-79 0.1589156261022211 1112 80-89 0.21964513592788393 1112 90-99 0.17960045488091225 1112 100-109 0.1666843555056161 1112 110-119 0.11548140709942345 1112 120-129 0.23172033951326654 1112 130-139 0.027473052272981136 1112 140-149 0.619298400017577 1112 150-159 0.3973323885873157 1112 160-169 0.3728025758363742 1112 170-179 0.3940447921585317 1112 180-189 0.31765208271269074 1112 190-199 0.3091423790612211 1112 200-209 -0.09799554459229043 1112 210-219 -0.08623082971890028 1112 220-229 0.18570245377372174 1112 230-239 -0.07077334244012334 1112 240-249 -0.02494472698004202 1112 250-259 -0.08870866927306409 1112 260-269 0.27503650172391403 1112 270-279 0.22141586782240807 1112 280-289 0.4141599229664621 1112 290-299 0.0698778428840825 1112 300-309 0.18743810756368617 1112 310-319 0.22396467100297457 1112 320-329 0.4279336536794176 1112 330-339 0.267637367037068 1112 340-349 0.25129557220807897 1112 350-351 0.21669575580752465 2114 1 0.2313887781945212 2114 2 0.43651321902711615 2114 3 0.1918317755556629 2114 4 0.4548138368637886 2114 5 0.3245738411175694 2114 6 0.2658809187118223 2114 7 0.09383690150797719 2114 8 0.13836276743820264 2114 9 0.12767160591582183 2114 10-19 0.07904339696563767 2114 20-29 0.1460341378779404 2114 30-39 0.04775150273844275 2114 40-49 0.03674324534611628 2114 50-59 0.005796726509863959 2114 60-69 0.042439363991611856 2114 70-79 0.10473063781484626 2114 80-89 0.058202144888639396 2114 90-99 0.1774491334794135 2114 100-109 0.1308224404439855 2114 110-119 0.1766095963819012 2114 120-129 0.18459633878435966 2114 130-139 -0.08747554155088721 2114 140-149 0.42194463626150736 2114 150-159 0.3320874796870399 2114 160-169 0.3525708849125522 2114 170-179 0.5651782369401417 2114 180-189 0.01814422455307607 2114 190-199 0.11444686789130287 2114 200-209 -0.41469561933129384 2114 210-219 0.3781654085057511 2114 220-229 0.48103720794048854 2114 230-239 0.18597495612504744 2114 240-249 -0.4523389501565305 2114 250-259 -0.3027430967537761 2114 260-269 -0.17744203391972135 2114 270-279 0.07348531963951643 2114 280-289 -0.09517703903812702 2114 290-299 -0.11209579510973455 2114 300-309 0.22841792803160388 2114 310-319 0.22562279418236741 2114 320-329 0.08392690340013687 2114 330-339 0.13853339002393739 2114 340-349 0.12466656555023192 2114 350-351 0.1025206975084565 1114 1 0.38194407564369115 1114 2 0.4435927084189437 1114 3 0.46766361555920355 1114 4 0.020293582887106254 1114 5 0.13975492118216692 1114 6 0.10892642655456797 1114 7 0.04802209652579137 1114 8 0.12577178493781105 1114 9 0.10608469412183297 1114 10-19 -0.06979595393367077 1114 20-29 0.16161233305060563 1114 30-39 0.1821113620335808 1114 40-49 0.1677414533503665 1114 50-59 0.01637199033147141 1114 60-69 -0.022553114050907652 1114 70-79 0.16819521824448458 1114 80-89 0.16279662288690844 1114 90-99 0.2957194704188666 1114 100-109 0.07240642088689953 1114 110-119 0.05485039857655494 1114 120-129 0.06741751530699958 1114 130-139 -0.07885681630143893 1114 140-149 0.3328993164676959 1114 150-159 0.1541212842488875 1114 160-169 0.41250374038034465 1114 170-179 0.38507077250351074 1114 180-189 0.24206847401658393 1114 190-199 0.2839619228679666 1114 200-209 -0.05013869581190633 1114 210-219 0.08118359394412522 1114 220-229 0.025857565897169366 1114 230-239 -0.2995815028028517 1114 240-249 -0.24831580907557793 1114 250-259 -0.3078173760838787 1114 260-269 0.0024278804627009265 1114 270-279 0.02156611431220057 1114 280-289 -0.07054594680565174 1114 290-299 -0.10887711631944441 1114 300-309 -0.017647147962282617 1114 310-319 0.10185838631528199 1114 320-329 -0.048621878706455846 1114 330-339 -0.026321093283954156 1114 340-349 -0.10246834296949103 1114 350-351 -0.21456714346851413 1109 1 0.23600426161608112 1109 2 -0.105490932478979 1109 3 0.37482286900242556 1109 4 0.31975897061852976 1109 5 0.33675129041971275 1109 6 0.1493015720017823 1109 7 0.11018973506321572 1109 8 0.07501154195345094 1109 9 0.2295531224917866 1109 10-19 0.004706983349819893 1109 20-29 -0.00969147408222426 1109 30-39 0.11068469576699869 1109 40-49 0.22181964310355085 1109 50-59 0.17160571441352346 1109 60-69 -0.06209283405877386 1109 70-79 0.18831456830800164 1109 80-89 0.1005541352978554 1109 90-99 0.23106243410789773 1109 100-109 -0.015011561889473057 1109 110-119 0.2194849301615136 1109 120-129 0.12248591300384959 1109 130-139 0.19608500481432856 1109 140-149 0.4832756871722097 1109 150-159 -0.16351708647563612 1109 160-169 0.32134768094119437 1109 170-179 0.36263401911863014 1109 180-189 0.01867839543341887 1109 190-199 0.07458528710778012 1109 200-209 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2105 140-149 -2.986379624616937 2105 150-159 0.05223626794728631 2105 160-169 -0.1781538341248634 2105 170-179 0.17566004099779065 2105 180-189 0.18584401702066344 2105 190-199 0.3061906504245542 2105 200-209 0.2980032658908094 2105 210-219 0.15348464551228957 2105 220-229 -0.3807618031619313 2105 230-239 -0.3275152172722535 2105 240-249 0.06885827367227648 2105 250-259 0.04061388166512003 2105 260-269 -0.04019507035697245 2105 270-279 -0.48136702615710547 2105 280-289 -0.25429485620547965 2105 290-299 -0.3052075337323146 2105 300-309 -0.391832641831968 2105 310-319 -0.22428846586821294 2105 320-329 -0.3095984824627145 2105 330-339 -0.24706947460369122 2105 340-349 -0.34016292900062695 2105 350-351 -0.12272425877365478 2104 1 -0.08302960003063475 2104 2 -0.37368403385218585 2104 3 -0.4190513331645036 2104 4 -0.35260733285010204 2104 5 -0.24318292346687898 2104 6 -0.01491018796248511 2104 7 -0.09313430240626985 2104 8 -0.14426776393061402 2104 9 -0.09985013133915288 2104 10-19 -0.003407813603807597 2104 20-29 -0.09572604058013923 2104 30-39 -0.05316697716346397 2104 40-49 0.07232887256837728 2104 50-59 0.15556872411892897 2104 60-69 -0.1791922087935376 2104 70-79 -0.03612677441963541 2104 80-89 0.07081930422533844 2104 90-99 -0.07977114764137383 2104 100-109 0.02613206152866354 2104 110-119 -0.05927397450527394 2104 120-129 -0.10746209144998886 2104 130-139 0.04585748367111364 2104 140-149 -2.611958394937851 2104 150-159 0.3710549369674041 2104 160-169 0.11113699636257479 2104 170-179 0.5150602302972587 2104 180-189 0.3239795452045513 2104 190-199 0.6207535988916142 2104 200-209 0.4375665341204744 2104 210-219 0.6578765849364672 2104 220-229 0.26403469991419115 2104 230-239 0.41100530509194755 2104 240-249 0.4287408568425235 2104 250-259 0.40730133290132287 2104 260-269 0.42411238778610283 2104 270-279 -0.11813620871597053 2104 280-289 -0.06791226663997207 2104 290-299 0.13441361152944253 2104 300-309 -0.1132359520144881 2104 310-319 -0.13864676626599248 2104 320-329 -0.15081172575089496 2104 330-339 -0.04858355055370822 2104 340-349 -0.11120714577409174 2104 350-351 0.1857107590690994 2111 1 -0.16188286244554106 2111 2 -0.2097370219599668 2111 3 -0.5064716753793661 2111 4 0.01826091610033842 2111 5 -0.04849344247602971 2111 6 -0.09608168009287965 2111 7 -0.009440522822075081 2111 8 -0.16663048492347343 2111 9 -0.021354806268490734 2111 10-19 -0.012389030256251488 2111 20-29 0.013244471404064484 2111 30-39 -0.18450606425597016 2111 40-49 -0.21008536464039196 2111 50-59 0.15146281480753743 2111 60-69 -0.24013823384914446 2111 70-79 -0.16488185495553154 2111 80-89 0.019279839124223486 2111 90-99 0.21591838352760817 2111 100-109 -0.10469905560382387 2111 110-119 -0.05530287710951143 2111 120-129 -0.15945031631492412 2111 130-139 0.021547014840095358 2111 140-149 -2.6447258861984935 2111 150-159 0.38565794431013956 2111 160-169 0.2510112165107614 2111 170-179 0.3074473713145309 2111 180-189 0.1520732779558145 2111 190-199 0.711849991083902 2111 200-209 -1.3159603195433718 2111 210-219 -0.3545664210681849 2111 220-229 0.09547715395937573 2111 230-239 -0.1708787165181036 2111 240-249 -0.2787607739467717 2111 250-259 -0.09342942796642362 2111 260-269 -0.07876649892839538 2111 270-279 -0.3608100341295213 2111 280-289 -0.34049610593613977 2111 290-299 -0.16295370912555285 2111 300-309 -0.3855419717177604 2111 310-319 -0.4112212389774541 2111 320-329 -0.41540218856053457 2111 330-339 -0.16158035587928055 2111 340-349 -0.12809225457701245 2111 350-351 -0.237888016584467 2110 1 0.32963060850718406 2110 2 0.008482142053587438 2110 3 0.23684419877762863 2110 4 0.15176541782913944 2110 5 0.042390128781370606 2110 6 -0.0643333661015788 2110 7 -0.05382791113469665 2110 8 0.01736184673240615 2110 9 -0.22251190462184667 2110 10-19 -0.12417226260023995 2110 20-29 -0.2830692460311468 2110 30-39 -0.2614921311485858 2110 40-49 -0.0519832798093276 2110 50-59 0.08361919425364306 2110 60-69 -0.2828557032955885 2110 70-79 -0.11787559019226279 2110 80-89 -0.15663471361276038 2110 90-99 -0.17644065929872 2110 100-109 -0.2860868889542729 2110 110-119 -0.15173161970648152 2110 120-129 -0.1490234223366258 2110 130-139 -0.03598336755010223 2110 140-149 0.04729835124630455 2110 150-159 -0.20343246683635385 2110 160-169 -0.3546263857623444 2110 170-179 -3.456216317022051 2110 180-189 -0.53503637251832 2110 190-199 -0.35428331486248865 2110 200-209 -2.295657693820175 2110 210-219 -1.7812700601082732 2110 220-229 -0.8214027885280366 2110 230-239 -0.48591072500805055 2110 240-249 -0.5062730124870463 2110 250-259 -0.4403569552677844 2110 260-269 -0.503216877210555 2110 270-279 -0.36014932412301803 2110 280-289 -0.4746325613810072 2110 290-299 -0.5753873813723089 2110 300-309 -0.5552362649634617 2110 310-319 -0.4978523026178614 2110 320-329 -0.22550112389311394 2110 330-339 -0.1529281358251957 2110 340-349 -0.20715630579059408 2110 350-351 -0.02215421767310133 2109 1 0.13240118050770633 2109 2 0.3110738528433039 2109 3 0.4845894814612599 2109 4 -0.05633900868969732 2109 5 -0.11285784866553072 2109 6 0.09087078442612295 2109 7 -0.10110057379771575 2109 8 -0.08556389625232441 2109 9 -0.09441292685512792 2109 10-19 -0.11579966271632713 2109 20-29 -0.16740135931781452 2109 30-39 -0.15185672373591075 2109 40-49 0.0021843048536211995 2109 50-59 0.008222797537094095 2109 60-69 0.06292601495654537 2109 70-79 -0.08396693970602342 2109 80-89 -0.07790285144248799 2109 90-99 0.07597799438569552 2109 100-109 -0.23991333435422746 2109 110-119 -0.12856838220011468 2109 120-129 -0.18006425244217183 2109 130-139 0.163140212516943 2109 140-149 0.4210569241470594 2109 150-159 0.0744364788301155 2109 160-169 -0.08246654780349161 2109 170-179 -3.330610261800132 2109 180-189 -0.263774157245237 2109 190-199 0.30791525661684815 2109 200-209 0.2717257206521815 2109 210-219 -0.08752913642634041 2109 220-229 0.06950952209821182 2109 230-239 0.014866906679994685 2109 240-249 -0.6323005168401252 2109 250-259 -0.2598491552606781 2109 260-269 0.2886388966899247 2109 270-279 -0.06190642822971881 2109 280-289 -0.1823673019137999 2109 290-299 -0.036978416990564256 2109 300-309 -0.16215487718998745 2109 310-319 -0.46095145806128457 2109 320-329 -0.18841727233607486 2109 330-339 -0.17053897647288352 2109 340-349 0.010557587342795216 2109 350-351 -0.47902438338623554 2108 1 0.09397220802330963 2108 2 -0.40662461592480525 2108 3 -0.16617481149168967 2108 4 -0.13693573137188153 2108 5 -0.32604358891970264 2108 6 -0.0862091587381073 2108 7 -0.26989461292126293 2108 8 -0.09029624157461313 2108 9 -0.19870841994777777 2108 10-19 -0.19277429565196513 2108 20-29 -0.4044765132480279 2108 30-39 -0.2941711938375988 2108 40-49 -0.18708873705084983 2108 50-59 -0.21013544775106396 2108 60-69 -0.10879408739018004 2108 70-79 -0.27652867716602714 2108 80-89 -0.3065398248582767 2108 90-99 -0.3821058886511537 2108 100-109 -0.2514340881426165 2108 110-119 -0.011308750150888613 2108 120-129 -0.07965174328043645 2108 130-139 -0.019525274040134377 2108 140-149 0.24995029357163645 2108 150-159 0.008909835077680839 2108 160-169 -0.41395364465451223 2108 170-179 -0.4264663836848186 2108 180-189 -0.25540292016756894 2108 190-199 -0.027184933629520458 2108 200-209 0.037168222061168876 2108 210-219 -0.2997423446574743 2108 220-229 -0.14342868619907279 2108 230-239 -0.14714031156884033 2108 240-249 -0.17991397008952958 2108 250-259 -0.24517306359314972 2108 260-269 -0.08595011559009436 2108 270-279 -0.31835482754070554 2108 280-289 -0.0919064770542306 2108 290-299 -0.09683458121101651 2108 300-309 -0.2144478883481078 2108 310-319 -0.39172427108799823 2108 320-329 -0.359760321728686 2108 330-339 -0.20751212765656746 2108 340-349 -0.0619818444582485 2108 350-351 0.24254053190253444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 27.0 18 137.0 19 436.0 20 1267.0 21 3153.0 22 5898.0 23 10349.0 24 15719.0 25 22033.0 26 27463.0 27 30401.0 28 31665.0 29 30301.0 30 34313.0 31 33949.0 32 33185.0 33 35819.0 34 39131.0 35 34057.0 36 18396.0 37 1809.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 99.68401105717103 0.13967981089502526 0.1470056751028063 0.02930345683112418 2 0.026861502095197165 0.08644519765181632 99.8026900573371 0.08400324291588931 3 99.34311417603563 0.09425945280678277 0.47007628666595036 0.09255008449163386 4 99.73944342967658 0.06837473260595642 0.17020424509411294 0.021977592623343135 5 0.004151323051075925 0.12234193226994343 0.09767818943708058 99.7758285552419 6 0.03662932103890522 0.10451566269767623 0.3274661300878127 99.5313888861756 7 0.2915693954696856 0.2605565703234125 99.22101643923928 0.22685759496761967 8 30.30050694980318 10.807847465739375 5.833585668656046 53.0580599158014 9 20.42914912529181 9.090909090909092 28.895406194750773 41.58453558904832 10-19 22.644807915840474 18.066899791945456 26.227448548013715 33.060843744200355 20-29 23.179107612061305 20.873902341346202 24.02504957168114 31.92194047491136 30-39 26.094630629926645 23.018378151342585 22.048873282084845 28.838117936645922 40-49 20.924865936685 24.34589800443459 28.06812076931342 26.661115289566993 50-59 21.139709114351856 19.5267735917247 28.28528380397941 31.04823348994403 60-69 22.993665569415008 21.770319505357648 26.853785518231632 28.382229406995712 70-79 23.891987458120475 17.648666204323238 26.747340711292576 31.71200562626371 80-89 23.633066997470134 24.633877726442464 20.254671459409828 31.478383816677574 90-99 23.529015306172283 21.429495882864316 23.215614835363414 31.82587397559999 100-109 21.943502935229592 21.988190706897058 24.9129443136642 31.155362044209152 110-119 22.55992556921965 22.273313341863897 23.601639040018753 31.565122048897702 120-129 20.33857702413628 27.438365062465202 22.28149389022925 29.941564023169263 130-139 23.773259618859704 24.0880275843207 23.204162067651914 28.934550729167686 140-149 22.948977797747542 24.746622776600212 23.88654189905936 28.417857526592886 150-159 22.404153276614867 22.817698311144106 23.67411625658107 31.10403215565996 160-169 24.972161716010433 20.963815114723033 29.011032751496916 25.052990417769617 170-179 20.58156157220937 24.13905180804859 26.210538602683513 29.068848017058528 180-189 21.69664573097473 26.104105414302044 24.460743135665236 27.738505719057994 190-199 19.439234261414736 26.73985044000372 21.101155801878303 32.71975949670324 200-209 19.488961990214104 24.24630843327244 25.565062634963887 30.699666941549573 210-219 18.359230676624307 24.854880699493222 24.67676447755953 32.10912414632294 220-229 17.859870869433564 26.796130966916397 26.000297918477784 29.343700245172254 230-239 20.088154565966963 27.572892348867423 25.68477294704865 26.654180138116963 240-249 18.510729585436568 28.48919398136153 24.776127640028434 28.223948793173463 250-259 17.821829566657932 30.139882527296784 24.224148056728577 27.814139849316703 260-269 17.02868247474591 30.39565292879576 23.711435115171163 28.864229481287175 270-279 18.913578956596442 28.20314333374277 24.31391433474177 28.56936337491902 280-289 15.942647260284845 27.48586779400349 25.436285844546592 31.135199101165078 290-299 15.88753620810153 30.083676061847907 23.49737904870188 30.531408681348683 300-309 14.71250866893931 30.97050607069947 24.795413032224033 29.521572228137178 310-319 13.754557604383214 31.52149201517236 22.35754182031653 32.3664085601279 320-329 11.447376582326394 34.83589323573324 22.74821138938013 30.96851879256024 330-339 12.711375601941842 35.44585209568555 20.700523555095383 31.14224874727722 340-349 9.944521216806807 39.04576688264134 20.545391187813472 30.464320712738385 350-351 7.586054484894069 41.634595661134824 16.772566103714702 34.0067837502564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.25 2 0.25 3 0.0 4 0.25 5 0.0 6 0.0 7 0.0 8 0.25 9 0.25 10 0.0 11 0.0 12 0.25 13 0.25 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.25 21 0.25 22 0.25 23 0.25 24 0.75 25 0.75 26 0.0 27 0.0 28 1.0 29 0.75 30 0.25 31 0.5 32 2.0 33 3.0 34 9.0 35 13.0 36 22.75 37 43.25 38 81.25 39 132.75 40 233.25 41 472.25 42 757.25 43 1309.5 44 1973.75 45 3083.5 46 4358.0 47 5839.5 48 7420.5 49 9176.25 50 11859.75 51 16462.25 52 17510.25 53 17183.75 54 16764.5 55 9285.75 56 3828.5 57 2303.0 58 1598.75 59 1309.5 60 1098.75 61 886.5 62 683.5 63 506.5 64 321.75 65 204.75 66 111.75 67 64.5 68 32.5 69 17.0 70 14.75 71 8.75 72 4.25 73 4.5 74 3.75 75 2.75 76 2.75 77 0.75 78 1.25 79 0.5 80 1.0 81 1.0 82 0.25 83 0.25 84 0.25 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.25 91 0.25 92 0.25 93 0.0 94 0.0 95 0.0 96 0.0 97 0.25 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-19 0.0 20-29 0.0 30-39 0.0 40-49 0.0 50-59 0.0 60-69 0.0 70-79 0.0 80-89 0.0 90-99 0.0 100-109 0.0 110-119 0.0 120-129 0.0 130-139 0.0 140-149 0.0 150-159 0.0 160-169 0.0 170-179 4.8839094718540294E-5 180-189 0.0 190-199 2.4419547359270147E-5 200-209 7.325864207781044E-5 210-219 2.4419547359270147E-5 220-229 0.0 230-239 0.0 240-249 2.4419547359270147E-5 250-259 2.4419547359270147E-5 260-269 2.4419547359270147E-5 270-279 2.4419547359270147E-5 280-289 2.4419547359270147E-5 290-299 4.8839094718540294E-5 300-309 0.0 310-319 2.4419547359270147E-5 320-329 4.8839094718540294E-5 330-339 0.0 340-349 2.4419547359270147E-5 350-351 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 351 409508.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.22177984147618 #Duplication Level Percentage of deduplicated Percentage of total 1 90.00830390786383 21.80161321160958 2 4.805804292980284 2.3281026709157904 3 1.3445799423080194 0.9770435802554875 4 0.7651971909948275 0.7413775158237085 5 0.4699344925537933 0.5691324909276905 6 0.29826936033425605 0.43347688676845675 7 0.26311275645433524 0.4461141482224594 8 0.15877515673683135 0.30766535126203215 9 0.17609237203607384 0.38387436004989867 >10 1.1575187292824305 5.85306164270729 >50 0.2000197485044092 3.4044047175185264 >100 0.2622910487638689 13.668130456084308 >500 0.04555668599344585 7.7963411116216585 >1k 0.03340823639519357 17.8251125396616 >5k 0.007086595598980455 12.021872659569825 >10k+ 0.004049483199417402 11.44267665700172 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGGCCTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT 13919 3.3989567969368117 No Hit GTGGCCTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA 11435 2.7923752405325417 No Hit GTGGCCTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT 10691 2.6106938081795716 No Hit GTGGCCTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 10619 2.5931117340808973 No Hit GTGGCCTCTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAG 9473 2.3132637213436613 No Hit GTGGCCTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA 7918 1.9335397599070103 No Hit GTGGCCTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG 7692 1.8783515828750597 No Hit GTGGCCTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT 6386 1.5594322943629917 No Hit GTGGCCTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA 6138 1.4988718169120017 No Hit GTGGCCTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC 5832 1.4241480019926351 No Hit GTGGCCTCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTT 5587 1.3643201109624232 No Hit GTGGCCTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC 4657 1.137218320521211 No Hit GTGGCCTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT 4633 1.1313576291549858 No Hit GTGGCCTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG 4449 1.086425662013929 No Hit GTGGCCTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT 3788 0.9250124539691533 No Hit GTGGCCTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT 3772 0.92110532639167 No Hit GTGGCCTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC 3441 0.8402766246324859 No Hit GTGGCCTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC 3264 0.7970540258065777 No Hit GTGGCCTCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC 3132 0.764820223292341 No Hit GTGGCCTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA 2907 0.7098762417339832 No Hit GTGGCCTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA 2894 0.7067017005772781 No Hit GTGGCCTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT 2323 0.5672660851558455 No Hit GTGGCCTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG 2042 0.49864715707629637 No Hit GTGGCCTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA 2022 0.49376324760444246 No Hit GTGGCCTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC 2001 0.48863514265899566 No Hit GTGGCCTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC 1914 0.4673901364564306 No Hit GTGGCCTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC 1823 0.44516834835949487 No Hit GTGGCCTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT 1764 0.4307608154175254 No Hit GTGGCCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCC 1644 0.40145735858640125 No Hit GTGGCCTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG 1623 0.39632925364095456 No Hit GTGGCCTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC 1553 0.37923557048946543 No Hit GTGGCCTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA 1522 0.3716655108080917 No Hit GTGGCCTCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC 1479 0.3611651054436055 No Hit GTGGCCTCCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACC 1469 0.35872315070767846 No Hit GTGGCCTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT 1408 0.34382722681852373 No Hit GTGGCCTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA 1390 0.33943170829385505 No Hit GTGGCCTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC 1389 0.3391875128202624 No Hit GTGGCCTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA 1374 0.33552458071637187 No Hit GTGGCCTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA 1257 0.30695371030602575 No Hit GTGGCCTGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAG 1224 0.2988952596774666 No Hit GTGGCCTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA 1222 0.2984068687302812 No Hit GTGGCCTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC 1208 0.2949881320999834 No Hit GTGGCCTCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGA 1097 0.26788243453119354 No Hit GTGGCCTCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT 1007 0.2459048419078504 No Hit GTGGCCTACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACA 965 0.23564863201695693 No Hit GTGGCCTGTCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAG 934 0.22807857233558318 No Hit GTGGCCTCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG 933 0.22783437686199046 No Hit GTGGCCTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC 898 0.21928753528624592 No Hit GTGGCCTCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTCA 893 0.21806655791828242 No Hit GTGGCCTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG 883 0.21562460318235543 No Hit GTGGCCTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA 876 0.21391523486720648 No Hit GTGGCCTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC 866 0.2114732801312795 No Hit GTGGCCTCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAA 860 0.21000810728972327 No Hit GTGGCCTAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT 855 0.20878712992175977 No Hit GTGGCCTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC 847 0.20683356613301818 No Hit GTGGCCTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT 844 0.20610097971224006 No Hit GTGGCCTCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACT 802 0.1958447698213466 No Hit GTGGCCTCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT 795 0.19413540150619768 No Hit GTGGCCTCTTCTCCCGAAGTTACGGTGCCATTTTGCCTAGTTCCTTCACC 787 0.19218183771745606 No Hit GTGGCCTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC 773 0.18876310108715827 No Hit GTGGCCTCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTA 770 0.18803051466638016 No Hit GTGGCCTACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCAC 764 0.18656534182482393 No Hit GTGGCCTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT 757 0.18485597350967503 No Hit GTGGCCTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC 749 0.1829024097209334 No Hit GTGGCCTCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTA 745 0.1819256278265626 No Hit GTGGCCTCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCT 744 0.1816814323529699 No Hit GTGGCCTCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACC 703 0.17166941793566914 No Hit GTGGCCTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA 699 0.17069263604129833 No Hit GTGGCCTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG 664 0.16214579446555377 No Hit GTGGCCTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT 647 0.15799447141447787 No Hit GTGGCCTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG 622 0.15188958457466034 No Hit GTGGCCTCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGAT 605 0.1477382615235844 No Hit GTGGCCTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG 601 0.1467614796292136 No Hit GTGGCCTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT 597 0.1457846977348428 No Hit GTGGCCTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC 594 0.14505211131406467 No Hit GTGGCCTCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACA 590 0.1440753294196939 No Hit GTGGCCTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT 589 0.14383113394610117 No Hit GTGGCCTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT 589 0.14383113394610117 No Hit GTGGCCTCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAAG 583 0.14236596110454497 No Hit GTGGCCTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA 562 0.13723785615909823 No Hit GTGGCCTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC 558 0.13626107426472742 No Hit GTGGCCTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA 557 0.13601687879113472 No Hit GTGGCCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT 551 0.13455170594957852 No Hit GTGGCCTCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGCG 538 0.1313771647928734 No Hit GTGGCCTCTTTCGGCGTGGGTGTTTTTCACACCCATTGTCGTTACTCATG 528 0.1289352100569464 No Hit GTGGCCTCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCC 527 0.1286910145833537 No Hit GTGGCCTCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCT 521 0.1272258417417975 No Hit GTGGCCTCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCC 517 0.12624905984742668 No Hit GTGGCCTGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT 512 0.12502808247946315 No Hit GTGGCCTGGCCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAG 500 0.12209773679635073 No Hit GTGGCCTCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTCG 498 0.12160934584916534 No Hit GTGGCCTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC 489 0.11941158658683104 No Hit GTGGCCTGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCCC 489 0.11941158658683104 No Hit GTGGCCTGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTT 475 0.1159928499565332 No Hit GTGGCCTCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTC 471 0.11501606806216241 No Hit GTGGCCTGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGA 458 0.11184152690545729 No Hit GTGGCCTCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC 446 0.10891118122234487 No Hit GTGGCCTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA 444 0.10842279027515946 No Hit GTGGCCTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCAC 437 0.10671342196001055 No Hit GTGGCCTCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCAT 433 0.10573664006563975 No Hit GTGGCCTCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTA 428 0.10451566269767623 No Hit GTGGCCTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG 423 0.10329468532971273 No Hit GTGGCCTGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA 418 0.10207370796174922 No Hit GTGGCCTCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAACC 417 0.10182951248815651 No Hit GTGGCCTCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTC 416 0.10158531701456382 No Hit GTGGCCTGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTTTTCCG 412 0.10060853512019301 No Hit GTGGCCTCGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT 412 0.10060853512019301 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 2.4419547359270146E-4 0.0 0.0 8 0.0014651728415562089 0.0 0.0 9 0.034675757250163614 0.0 0.0 10-19 0.039071275774832236 0.0 0.0 20-29 0.04151323051075926 0.0 0.0 30-39 0.04156206960547779 0.0 0.0 40-49 0.04971819842347402 0.0 0.0 50-59 0.13027828516170623 0.0 0.0 60-69 0.5331763970423045 0.0 0.0 70-79 1.425857370307784 0.0 0.0 80-89 2.9444601814860762 0.0 0.0 90-99 5.613663225138459 0.0 0.0 100-109 8.856139562597068 0.0 0.0 110-119 12.592623343133711 0.0 0.0 120-129 16.528761342879747 0.0 0.0 130-139 20.56211356066304 0.0 0.0 140-149 23.529137403909083 0.0 0.0 150-159 26.32559070885062 0.0 0.0 160-169 29.89697392969124 0.0 0.0 170-179 33.81042616994051 0.0 0.0 180-189 38.00336012971664 0.0 0.0 190-199 41.25912070093869 2.4419547359270147E-5 0.0 200-209 43.73311388300106 2.4419547359270146E-4 0.0 210-219 45.76784824716489 2.4419547359270146E-4 0.0 220-229 48.69247975619523 2.4419547359270146E-4 0.0 230-239 52.37209041093215 2.4419547359270146E-4 0.0 240-249 54.99396837180226 2.4419547359270146E-4 0.0 250-259 58.37473260595641 2.4419547359270146E-4 1.465172841556209E-4 260-269 61.102762339197284 2.4419547359270146E-4 2.930345683112418E-4 270-279 62.66104691483439 2.4419547359270146E-4 6.837473260595642E-4 280-289 63.993011125545785 2.4419547359270146E-4 9.767818943708058E-4 290-299 65.6569835021538 2.4419547359270146E-4 0.0011721382732449669 300-309 66.87913300839057 2.4419547359270146E-4 0.001489592388915479 310-319 67.66807974447384 2.4419547359270146E-4 0.0018070465045859911 320-329 68.29720054309072 2.4419547359270146E-4 0.0019535637887416117 330-339 68.74036648856676 2.4419547359270146E-4 0.0019535637887416117 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGCCT 40205 0.0 343.28293 1 TGGCCTC 21785 0.0 341.83185 2 GGCCTCC 8555 0.0 340.96643 3 GGCCTCT 6750 0.0 337.8436 3 GGCCTCG 4610 0.0 327.0382 3 TGGCCTG 12955 0.0 323.82782 2 GCCTCTC 4735 0.0 321.31912 4 GGCCTGT 3800 0.0 319.1242 3 GGCCTCA 2385 0.0 318.9615 3 GGCCTGC 6560 0.0 317.9146 3 CTGCCCC 2325 0.0 314.57986 6 GCCTGCC 5430 0.0 313.54895 4 GCCTGTC 2770 0.0 313.2393 4 GGCCTAT 1340 0.0 310.24176 3 CCTGCCC 3580 0.0 308.37912 5 TGGCCTT 2515 0.0 307.2756 2 GCCTCCG 1080 0.0 300.27704 4 GGCCTAC 1645 0.0 299.90808 3 GGCCTAA 455 0.0 299.50473 3 TCCCGTT 1220 0.0 298.33945 7 >>END_MODULE