FastQCFastQC Report
Mon 27 Jul 2015
000000000-AGWYV l01n01 ca65a.3410000000eafb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGWYV l01n01 ca65a.3410000000eafb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409508
Sequences flagged as poor quality0
Sequence length351
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCCTGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCT139193.3989567969368117No Hit
GTGGCCTCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTA114352.7923752405325417No Hit
GTGGCCTCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTAT106912.6106938081795716No Hit
GTGGCCTCGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA106192.5931117340808973No Hit
GTGGCCTCTCGTCATCAGCTCTCGGCCTTGAAATCCCGGATTTACCTAAG94732.3132637213436613No Hit
GTGGCCTGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAA79181.9335397599070103No Hit
GTGGCCTGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTG76921.8783515828750597No Hit
GTGGCCTCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTT63861.5594322943629917No Hit
GTGGCCTGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAA61381.4988718169120017No Hit
GTGGCCTCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCC58321.4241480019926351No Hit
GTGGCCTCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTT55871.3643201109624232No Hit
GTGGCCTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCAC46571.137218320521211No Hit
GTGGCCTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTT46331.1313576291549858No Hit
GTGGCCTGCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTG44491.086425662013929No Hit
GTGGCCTGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGAT37880.9250124539691533No Hit
GTGGCCTGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCT37720.92110532639167No Hit
GTGGCCTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCAC34410.8402766246324859No Hit
GTGGCCTCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATAC32640.7970540258065777No Hit
GTGGCCTCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAAC31320.764820223292341No Hit
GTGGCCTACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCA29070.7098762417339832No Hit
GTGGCCTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTA28940.7067017005772781No Hit
GTGGCCTCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGT23230.5672660851558455No Hit
GTGGCCTCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAG20420.49864715707629637No Hit
GTGGCCTCCCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAA20220.49376324760444246No Hit
GTGGCCTCCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTC20010.48863514265899566No Hit
GTGGCCTCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGC19140.4673901364564306No Hit
GTGGCCTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC18230.44516834835949487No Hit
GTGGCCTCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTT17640.4307608154175254No Hit
GTGGCCTCGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCC16440.40145735858640125No Hit
GTGGCCTGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATG16230.39632925364095456No Hit
GTGGCCTGCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTAC15530.37923557048946543No Hit
GTGGCCTCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACA15220.3716655108080917No Hit
GTGGCCTCTCTCGTACTAGGAGCAGCCCCTCTCAAATCTCAAACGTCCAC14790.3611651054436055No Hit
GTGGCCTCCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCACC14690.35872315070767846No Hit
GTGGCCTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTT14080.34382722681852373No Hit
GTGGCCTCTCCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAA13900.33943170829385505No Hit
GTGGCCTCCCCATTCAGACATCTCCGGATCACAGTCTGTTTGCCGACTCC13890.3391875128202624No Hit
GTGGCCTTGCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCA13740.33552458071637187No Hit
GTGGCCTCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATA12570.30695371030602575No Hit
GTGGCCTGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAG12240.2988952596774666No Hit
GTGGCCTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCA12220.2984068687302812No Hit
GTGGCCTATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGC12080.2949881320999834No Hit
GTGGCCTCCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGA10970.26788243453119354No Hit
GTGGCCTCGTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCT10070.2459048419078504No Hit
GTGGCCTACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTCACA9650.23564863201695693No Hit
GTGGCCTGTCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAG9340.22807857233558318No Hit
GTGGCCTCGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAG9330.22783437686199046No Hit
GTGGCCTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC8980.21928753528624592No Hit
GTGGCCTCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTTCA8930.21806655791828242No Hit
GTGGCCTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGTAG8830.21562460318235543No Hit
GTGGCCTGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAA8760.21391523486720648No Hit
GTGGCCTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGC8660.2114732801312795No Hit
GTGGCCTCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTAA8600.21000810728972327No Hit
GTGGCCTAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAAT8550.20878712992175977No Hit
GTGGCCTCCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTC8470.20683356613301818No Hit
GTGGCCTGCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGT8440.20610097971224006No Hit
GTGGCCTCCCATGGATAGATCGCCCGGTTTCGGGTCTATTCCCAGCGACT8020.1958447698213466No Hit
GTGGCCTCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT7950.19413540150619768No Hit
GTGGCCTCTTCTCCCGAAGTTACGGTGCCATTTTGCCTAGTTCCTTCACC7870.19218183771745606No Hit
GTGGCCTGTTTCCCTTTTCACGACGGACGTTAGCACCCGCCGTGTGTCTC7730.18876310108715827No Hit
GTGGCCTCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACTA7700.18803051466638016No Hit
GTGGCCTACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCCAACCAC7640.18656534182482393No Hit
GTGGCCTCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATT7570.18485597350967503No Hit
GTGGCCTCTCCGGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTTCGC7490.1829024097209334No Hit
GTGGCCTCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTA7450.1819256278265626No Hit
GTGGCCTCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTTTCT7440.1816814323529699No Hit
GTGGCCTCCTTTATACTCTACGACCGATTTCCGACCGGTCTGAGCGCACC7030.17166941793566914No Hit
GTGGCCTGCCCCTCTCAAATCTCAAACGTCCACGGCAGATAGGGACCGAA6990.17069263604129833No Hit
GTGGCCTCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAG6640.16214579446555377No Hit
GTGGCCTTGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT6470.15799447141447787No Hit
GTGGCCTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG6220.15188958457466034No Hit
GTGGCCTCCCCATATTCAGACAAGGTTTCTCGTGCCCCGTCCTACTCGAT6050.1477382615235844No Hit
GTGGCCTCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAG6010.1467614796292136No Hit
GTGGCCTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGT5970.1457846977348428No Hit
GTGGCCTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACC5940.14505211131406467No Hit
GTGGCCTCCCCGTCCTACTCGATTTCATTGCAAAGGGATTTTCGCGTACA5900.1440753294196939No Hit
GTGGCCTGCATCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGT5890.14383113394610117No Hit
GTGGCCTCCCACTTAACCATGACTTTGGGACCTTAGCTGACGGTCTGGGT5890.14383113394610117No Hit
GTGGCCTCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAAG5830.14236596110454497No Hit
GTGGCCTGTCGTTACTCATGTCAGCATTCGCACTTCTGATACCTCCAGCA5620.13723785615909823No Hit
GTGGCCTGACGGTCTGGGTTGTTTCCCTTTTCACGACGGACGTTAGCACC5580.13626107426472742No Hit
GTGGCCTAGCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTA5570.13601687879113472No Hit
GTGGCCTCCCATGCTCGGCACTTGTAGGTATTCGGAGTTTGCATCGGTTT5510.13455170594957852No Hit
GTGGCCTCTCGCCTTAGGGGCCGACTAACCCTGCGTCGATTAACGTTGCG5380.1313771647928734No Hit
GTGGCCTCTTTCGGCGTGGGTGTTTTTCACACCCATTGTCGTTACTCATG5280.1289352100569464No Hit
GTGGCCTCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCC5270.1286910145833537No Hit
GTGGCCTCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCT5210.1272258417417975No Hit
GTGGCCTCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCC5170.12624905984742668No Hit
GTGGCCTGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGT5120.12502808247946315No Hit
GTGGCCTGGCCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAG5000.12209773679635073No Hit
GTGGCCTCGCTAATCTCAATACAACTTAAGGGCTGGTCCCCGTTCGCTCG4980.12160934584916534No Hit
GTGGCCTCGGTTTGGTAAGTCGGGATGACCCCCTAGCCGAAACAGTGCTC4890.11941158658683104No Hit
GTGGCCTGGTTGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAGTTCCC4890.11941158658683104No Hit
GTGGCCTGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTAAATGCTT4750.1159928499565332No Hit
GTGGCCTCGCGCTTTTGCCTTTATACTCTACGACCGATTTCCGACCGGTC4710.11501606806216241No Hit
GTGGCCTGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGA4580.11184152690545729No Hit
GTGGCCTCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAAC4460.10891118122234487No Hit
GTGGCCTCCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGA4440.10842279027515946No Hit
GTGGCCTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCAC4370.10671342196001055No Hit
GTGGCCTCTGGTTACCTGAAGCTTAGAAGCTTTTCTTGGAAGCATGGCAT4330.10573664006563975No Hit
GTGGCCTCGCAGCGGCCTGGTATCTTCGACCGGCGTGGGCTTACGCAGTA4280.10451566269767623No Hit
GTGGCCTCGCGGATACACTGCATCTTCACAGCGATTTCAATTTCACTGAG4230.10329468532971273No Hit
GTGGCCTGGCCCTTTAGGGAACTCAAGGTTCCAGTGAGATCTCATCTTGA4180.10207370796174922No Hit
GTGGCCTCCTGGTTCGCCTCTTGCACCTATGTATTCAGTACAAGATAACC4170.10182951248815651No Hit
GTGGCCTCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTC4160.10158531701456382No Hit
GTGGCCTGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTTTTCCG4120.10060853512019301No Hit
GTGGCCTCGCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATT4120.10060853512019301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCCT402050.0343.282931
TGGCCTC217850.0341.831852
GGCCTCC85550.0340.966433
GGCCTCT67500.0337.84363
GGCCTCG46100.0327.03823
TGGCCTG129550.0323.827822
GCCTCTC47350.0321.319124
GGCCTGT38000.0319.12423
GGCCTCA23850.0318.96153
GGCCTGC65600.0317.91463
CTGCCCC23250.0314.579866
GCCTGCC54300.0313.548954
GCCTGTC27700.0313.23934
GGCCTAT13400.0310.241763
CCTGCCC35800.0308.379125
TGGCCTT25150.0307.27562
GCCTCCG10800.0300.277044
GGCCTAC16450.0299.908083
GGCCTAA4550.0299.504733
TCCCGTT12200.0298.339457
Produced by FastQC (version 0.11.2)