##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AGRGJ l01n03.3330000000e383.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16154709 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.844484354376178 32.0 32.0 33.0 29.0 33.0 2 31.85378046735475 33.0 32.0 33.0 30.0 34.0 3 31.565022310213077 33.0 32.0 33.0 29.0 34.0 4 31.83340987448304 33.0 32.0 33.0 30.0 34.0 5 32.249960367593125 33.0 32.0 33.0 32.0 34.0 6 36.31493207336635 37.0 37.0 37.0 34.0 37.0 7 36.48926928983988 37.0 37.0 37.0 37.0 37.0 8 36.59837487632863 37.0 37.0 37.0 37.0 37.0 9 36.63881206402418 37.0 37.0 37.0 37.0 37.0 10-11 36.583667864274126 37.0 37.0 37.0 37.0 37.0 12-13 37.04606802264281 37.5 37.5 37.5 37.0 37.5 14-15 37.35636284132385 38.0 38.0 38.0 37.0 38.0 16-17 37.22444260679657 38.0 38.0 38.0 36.5 38.0 18-19 37.59790922262976 38.0 38.0 38.0 38.0 38.0 20-21 37.034030665609635 38.0 38.0 38.0 37.0 38.0 22-23 36.99649817276189 38.5 38.0 38.5 35.0 38.5 24-25 37.34296278564968 38.5 38.0 39.0 36.0 39.0 26-27 36.74266156697716 38.0 37.5 39.0 34.0 39.0 28-29 38.16444957318637 39.0 38.5 39.0 37.5 39.0 30-31 36.958673226487704 39.0 37.5 39.0 34.0 39.0 32-33 36.55057825554147 38.5 37.0 39.0 33.0 39.0 34-35 36.51859005940621 38.5 37.0 39.0 33.0 39.0 36-37 36.74139555840962 39.0 37.0 39.0 33.0 39.0 38-39 37.22584705796929 39.0 38.0 39.0 35.0 39.0 40-41 36.675108694313224 38.5 37.0 39.0 33.5 39.0 42-43 37.39881702604485 38.5 37.5 39.0 35.5 39.0 44-45 36.93656091236308 38.5 37.5 39.0 34.5 39.0 46-47 36.833446705849056 39.0 37.5 39.0 33.0 39.0 48-49 36.663393998616755 38.5 37.0 39.0 33.5 39.0 50-51 36.537424072448474 38.5 37.0 39.0 33.0 39.0 52-53 36.971279581699676 39.0 38.0 39.0 34.5 39.0 54-55 36.740375267669634 38.0 37.5 39.0 34.0 39.0 56-57 36.80187089721022 38.5 37.5 39.0 33.0 39.0 58-59 36.12356465226331 38.0 37.0 39.0 32.0 39.0 60-61 36.046182478434 38.0 36.5 39.0 32.0 39.0 62-63 36.30253636880739 38.0 37.0 39.0 32.5 39.0 64-65 36.80336120570169 39.0 37.5 39.0 33.0 39.0 66-67 36.40570829842865 39.0 37.0 39.0 32.5 39.0 68-69 36.10650807761378 38.0 37.0 39.0 32.0 39.0 70-71 36.044142794525115 38.0 37.0 39.0 31.5 39.0 72-73 36.035992044177334 38.0 36.5 39.0 31.5 39.0 74-75 36.40171221901923 38.5 37.0 39.0 32.5 39.0 76-77 36.48669508686291 39.0 37.0 39.0 33.0 39.0 78-79 36.33098045901043 39.0 37.0 39.0 32.0 39.0 80-81 36.37248755146255 39.0 37.0 39.0 32.5 39.0 82-83 36.300994063093306 39.0 37.0 39.0 32.0 39.0 84-85 36.268089292106716 39.0 37.0 39.0 32.0 39.0 86-87 36.35947338946186 39.0 37.0 39.0 32.5 39.0 88-89 36.63508491548811 39.0 37.0 39.0 33.0 39.0 90-91 36.08742172328824 38.0 37.0 39.0 31.5 39.0 92-93 36.22823537087545 38.5 37.0 39.0 32.0 39.0 94-95 36.2766883327951 39.0 37.0 39.0 31.5 39.0 96-97 36.07625767198901 38.5 37.0 39.0 30.0 39.0 98-99 36.11396135331191 38.0 37.0 39.0 31.0 39.0 100-101 35.713646714403836 38.5 36.5 39.0 30.5 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5564028157914578 1101 2 0.3890100098770404 1101 3 0.542037894230802 1101 4 0.40705086859331985 1101 5 0.26225697170895756 1101 6 0.09040264711939017 1101 7 0.05905103528096589 1101 8 0.057674766950917444 1101 9 0.07304243005428646 1101 10-11 0.06615086523760993 1101 12-13 0.07586455686681859 1101 14-15 0.16494863568596685 1101 16-17 0.11063374826094474 1101 18-19 0.09354082850300927 1101 20-21 0.16999723419297652 1101 22-23 0.39141377413650247 1101 24-25 0.2418558003850606 1101 26-27 0.3945828817277288 1101 28-29 0.14772649618961964 1101 30-31 0.34879002481384447 1101 32-33 0.3876147728577948 1101 34-35 0.5135474690840098 1101 36-37 0.3352972254462543 1101 38-39 0.34978092619423506 1101 40-41 0.3878433191574544 1101 42-43 0.32398808458696493 1101 44-45 0.39274988603757066 1101 46-47 0.4681539421628571 1101 48-49 0.40307260476240714 1101 50-51 0.5302588478710746 1101 52-53 0.36216082315791454 1101 54-55 0.380963409138559 1101 56-57 0.2884639330050547 1101 58-59 0.45020446974994854 1101 60-61 0.4389079979249715 1101 62-63 0.4647843420900273 1101 64-65 0.2802866380116811 1101 66-67 0.4350571948776576 1101 68-69 0.45052574972847026 1101 70-71 0.5302164992953848 1101 72-73 0.3701272649751104 1101 74-75 0.3844807848183365 1101 76-77 0.390972602702945 1101 78-79 0.5167198606456651 1101 80-81 0.35505719333551866 1101 82-83 0.44656597663794884 1101 84-85 0.4781130991611846 1101 86-87 0.4781048353431956 1101 88-89 0.39710148735162676 1101 90-91 0.4726564489762808 1101 92-93 0.3850411140394243 1101 94-95 0.5531998757885574 1101 96-97 0.6027125939264195 1101 98-99 0.4410434077951848 1101 100-101 0.41186529762205737 1102 1 0.5536705692676058 1102 2 0.31251349224950786 1102 3 0.6412869559079297 1102 4 0.46310902966099476 1102 5 0.2978108030731903 1102 6 0.09740717536887189 1102 7 0.04976077190826089 1102 8 0.06540361371991565 1102 9 0.05582023328312857 1102 10-11 0.06723092607301595 1102 12-13 0.07535601515393608 1102 14-15 0.1613969326704705 1102 16-17 0.1496936583873918 1102 18-19 0.09829278323498158 1102 20-21 0.17403606706612607 1102 22-23 0.39389569111258993 1102 24-25 0.17765184868486728 1102 26-27 0.30479832324360956 1102 28-29 0.11543633117089769 1102 30-31 0.2459492237571439 1102 32-33 0.18006212014040557 1102 34-35 0.37083949041220166 1102 36-37 0.3090120002329684 1102 38-39 0.4804187835663285 1102 40-41 0.3039325075480619 1102 42-43 0.24674063052176365 1102 44-45 0.1852460484985059 1102 46-47 0.3722496010255085 1102 48-49 0.33973027576322323 1102 50-51 0.4343230535577405 1102 52-53 0.3171488143296628 1102 54-55 0.42110992277286385 1102 56-57 0.2238977501173096 1102 58-59 0.3920394683826913 1102 60-61 0.29121420773959983 1102 62-63 0.3578789462695866 1102 64-65 0.3993731911729981 1102 66-67 0.33597122436034255 1102 68-69 0.3023319998581684 1102 70-71 0.3553896349300416 1102 72-73 0.23373727653342513 1102 74-75 0.36696380872810863 1102 76-77 0.5349867976440166 1102 78-79 0.5953938213419576 1102 80-81 0.42339122975079846 1102 82-83 0.31882167282160623 1102 84-85 0.3741878519948969 1102 86-87 0.3975192440150508 1102 88-89 0.34723856915157825 1102 90-91 0.5220720294763481 1102 92-93 0.4460931885017061 1102 94-95 0.6068260918485393 1102 96-97 0.5719511206929226 1102 98-99 0.44998204845744283 1102 100-101 0.6593485657889957 1103 1 0.3724051569266962 1103 2 0.39428057697963226 1103 3 0.23448613537248875 1103 4 0.3301127106676809 1103 5 0.22100500393636224 1103 6 0.07877850790139718 1103 7 0.06615065302199952 1103 8 0.058650524497700474 1103 9 0.05010507700515632 1103 10-11 0.0992277491172402 1103 12-13 0.05497921959945273 1103 14-15 0.16626121683424344 1103 16-17 0.15942934318727708 1103 18-19 0.11652653822294212 1103 20-21 0.2916286114572628 1103 22-23 0.3503720500149825 1103 24-25 0.2622350100687214 1103 26-27 0.4582580115311927 1103 28-29 0.1699524264921095 1103 30-31 0.4615285951866497 1103 32-33 0.15414844547111528 1103 34-35 0.4968151625667119 1103 36-37 0.1799798126801022 1103 38-39 0.07413922495590697 1103 40-41 -0.5991302908711091 1103 42-43 -0.06594517060363358 1103 44-45 0.05724185848887231 1103 46-47 0.5049513349053498 1103 48-49 0.5057119743887739 1103 50-51 0.1940142112203347 1103 52-53 0.4407141099621441 1103 54-55 0.4559600268495956 1103 56-57 0.45592137651985354 1103 58-59 0.5222037903260528 1103 60-61 0.267264817738166 1103 62-63 0.41602308599532734 1103 64-65 0.45692558978202413 1103 66-67 0.5185054727368907 1103 68-69 0.456081257976642 1103 70-71 0.5491159549291709 1103 72-73 0.5479956603625311 1103 74-75 0.45930667758496213 1103 76-77 0.5313555304825073 1103 78-79 0.3527790539690301 1103 80-81 0.528447602706045 1103 82-83 0.05351821589417227 1103 84-85 0.15770464150589447 1103 86-87 0.3748723430413605 1103 88-89 -0.02753589946801327 1103 90-91 -0.43728041852507005 1103 92-93 -0.5844161067410454 1103 94-95 -0.9436679161821573 1103 96-97 -2.1490117682110466 1103 98-99 0.0411542704385468 1103 100-101 0.30922370479552796 2112 1 0.7470767513877696 2112 2 0.30851018647639705 2112 3 0.40779804074587744 2112 4 0.2555922791912657 2112 5 0.13802881134637346 2112 6 0.05926741039251482 2112 7 0.04267067727321461 2112 8 0.03717412575951329 2112 9 0.014996480210754726 2112 10-11 0.04344877166094818 2112 12-13 0.03231838977003321 2112 14-15 0.03980508139996175 2112 16-17 0.04295591317578129 2112 18-19 0.06015044284882265 2112 20-21 0.11115867426636328 2112 22-23 -0.7676782504491726 2112 24-25 -0.17709512302412378 2112 26-27 0.06923309601571503 2112 28-29 0.0851846224383479 2112 30-31 0.1952768796291764 2112 32-33 0.00423100914180452 2112 34-35 -0.5903149134835886 2112 36-37 0.10035667537614756 2112 38-39 0.23983740070787718 2112 40-41 0.34971102880899707 2112 42-43 0.2924069273032117 2112 44-45 0.2196805613072499 2112 46-47 0.5391785606458512 2112 48-49 0.43915256253374224 2112 50-51 0.06801433196224593 2112 52-53 0.36000248553811787 2112 54-55 0.32738806924240293 2112 56-57 0.350614399567732 2112 58-59 0.4787419117246756 2112 60-61 0.4393889155222581 2112 62-63 0.3684104832063113 2112 64-65 0.28113203958701405 2112 66-67 0.488773636776223 2112 68-69 0.4236531743252243 2112 70-71 0.6250880186573866 2112 72-73 0.4188386069641652 2112 74-75 0.4157491611884865 2112 76-77 0.3877616342456207 2112 78-79 0.44679273274444853 2112 80-81 0.30291996546844757 2112 82-83 0.15950903477263978 2112 84-85 0.3066196467954754 2112 86-87 0.2170085249186542 2112 88-89 0.33440329035807537 2112 90-91 0.11602339154728014 2112 92-93 -0.0664500257651639 2112 94-95 -0.7798852713611168 2112 96-97 -2.1977109833358597 2112 98-99 -0.12028999703380094 2112 100-101 0.32988318109107695 1113 1 0.6780861510117973 1113 2 0.2845172296471645 1113 3 0.14254667178649782 1113 4 0.16483433161101146 1113 5 0.1054309815646377 1113 6 0.032596445539532226 1113 7 -0.01559768151943075 1113 8 0.04021744728262178 1113 9 0.024042888588027722 1113 10-11 0.028207186241807847 1113 12-13 0.05061791910356561 1113 14-15 0.030813175921352354 1113 16-17 0.0675033598264676 1113 18-19 0.020780909950950388 1113 20-21 0.09526945770396367 1113 22-23 -0.6439572285123916 1113 24-25 0.09659442206981339 1113 26-27 -0.002966483064973602 1113 28-29 0.06831273411675909 1113 30-31 0.07526701189136986 1113 32-33 0.23318919515078562 1113 34-35 -0.8672497570712352 1113 36-37 -0.022802797305686795 1113 38-39 0.17839986643008388 1113 40-41 0.3491778557139682 1113 42-43 0.33213328963351074 1113 44-45 0.17445969385321547 1113 46-47 0.5269737895780509 1113 48-49 0.29838410917600555 1113 50-51 -0.11720558240625678 1113 52-53 0.14222764794353537 1113 54-55 0.2953614744925588 1113 56-57 0.14206169628734955 1113 58-59 0.4506224889085715 1113 60-61 0.34133444717700456 1113 62-63 0.323976041085551 1113 64-65 0.18014571113411648 1113 66-67 0.42317326971544134 1113 68-69 0.2397271342350038 1113 70-71 0.46388513771131556 1113 72-73 0.19126920552315596 1113 74-75 0.3092856320053414 1113 76-77 0.13204576242902277 1113 78-79 0.3310937483478469 1113 80-81 0.25636901930207756 1113 82-83 0.24628811098388326 1113 84-85 0.2866479254343446 1113 86-87 0.25051766068846604 1113 88-89 0.1397287853360325 1113 90-91 -0.3554901302777793 1113 92-93 0.11327969775410196 1113 94-95 -0.14999615714392434 1113 96-97 0.1223878437036916 1113 98-99 0.25694540521558196 1113 100-101 0.3043226969370565 2113 1 0.2639645350452078 2113 2 0.11478688824282557 2113 3 0.30444863940229894 2113 4 0.27149419095959715 2113 5 0.1040465306393159 2113 6 0.0403044635741594 2113 7 0.020925555590970646 2113 8 0.05223647026942757 2113 9 -0.021748803400164718 2113 10-11 0.028236005066595737 2113 12-13 0.04206190659053988 2113 14-15 0.019166809579360233 2113 16-17 0.08490853119891284 2113 18-19 0.049831264607448134 2113 20-21 0.23911492907078014 2113 22-23 -0.48881727361458616 2113 24-25 0.2653171439311919 2113 26-27 0.19907408572486673 2113 28-29 0.0879930149554724 2113 30-31 0.18871075899762957 2113 32-33 0.2887744748889318 2113 34-35 0.17500320831232585 2113 36-37 0.3454870628905411 2113 38-39 0.20687746281861052 2113 40-41 0.29087520057441907 2113 42-43 0.2144634305621267 2113 44-45 0.0693852342643666 2113 46-47 0.3534444503666947 2113 48-49 0.23691219423624688 2113 50-51 -0.08756843058367991 2113 52-53 0.06510445601492165 2113 54-55 0.1821042622375444 2113 56-57 0.08133635054632293 2113 58-59 0.2305016289626991 2113 60-61 0.21977822866958263 2113 62-63 0.025863165627619367 2113 64-65 -0.03286472341733315 2113 66-67 0.1608783211042777 2113 68-69 0.053335294471452244 2113 70-71 0.1749726572642274 2113 72-73 -0.054397368382502975 2113 74-75 0.08745791548059856 2113 76-77 0.046622817593849675 2113 78-79 0.06861056525926301 2113 80-81 0.008538739288219688 2113 82-83 -0.18826309861201196 2113 84-85 -0.33248744076814774 2113 86-87 -0.029097488534567617 2113 88-89 0.12812538529048112 2113 90-91 0.19416176870252144 2113 92-93 0.3644389607990064 2113 94-95 0.40582487164366654 2113 96-97 0.39587864464756706 2113 98-99 0.1481369108088586 2113 100-101 0.2569345868016768 1112 1 0.17989413263012466 1112 2 0.14695219245833613 1112 3 0.27675159646163294 1112 4 0.19654424877148458 1112 5 0.13122288392305848 1112 6 0.03232670527492587 1112 7 0.019716269368359463 1112 8 0.04497916781112821 1112 9 -0.047847423173521975 1112 10-11 0.014016039984035444 1112 12-13 0.04612793474868937 1112 14-15 0.05789768753940905 1112 16-17 0.03744212734509489 1112 18-19 0.054844484872575094 1112 20-21 0.211366862552957 1112 22-23 0.2015273595777245 1112 24-25 0.31357944075188726 1112 26-27 0.24196219624307957 1112 28-29 0.05658690095508234 1112 30-31 0.16339685639759693 1112 32-33 0.31820481689756974 1112 34-35 0.5072718599759227 1112 36-37 0.36764799735151144 1112 38-39 0.2087646753292347 1112 40-41 0.2781430453036364 1112 42-43 0.1078799554048615 1112 44-45 -0.08437474337932827 1112 46-47 0.18147325080683174 1112 48-49 0.2121405439208175 1112 50-51 0.3242838181414598 1112 52-53 0.21839548574666168 1112 54-55 0.20656912814696682 1112 56-57 0.12817072850970845 1112 58-59 0.39268782882695774 1112 60-61 0.11224799460568846 1112 62-63 0.301162382153521 1112 64-65 -0.037649408810629836 1112 66-67 0.035723119178008744 1112 68-69 0.051401585168143527 1112 70-71 0.2540653069838328 1112 72-73 -0.02355257745719541 1112 74-75 0.08191427094932635 1112 76-77 0.07647429920663029 1112 78-79 0.3222549857712451 1112 80-81 0.10691733710675067 1112 82-83 0.19652702471343275 1112 84-85 -0.3460053180099081 1112 86-87 0.10864630035479905 1112 88-89 0.2649305093456249 1112 90-91 0.24240636180058317 1112 92-93 0.4653066791340166 1112 94-95 0.45437361503463336 1112 96-97 0.5273023418447522 1112 98-99 0.28763478919727703 1112 100-101 0.2159071969364419 2114 1 0.5364829049110824 2114 2 0.16328091694050073 2114 3 0.25331281903102365 2114 4 0.25518075204187696 2114 5 0.16161202944238795 2114 6 0.06487661745514828 2114 7 0.03280940045291203 2114 8 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-0.10392366431157285 2110 90-91 -0.017721229514052084 2110 92-93 0.05191642390254003 2110 94-95 0.09697618009190734 2110 96-97 -0.081309753685332 2110 98-99 -0.4059354106035826 2110 100-101 -0.09231938801594453 2109 1 -0.4335331107547624 2109 2 -0.3590169534800083 2109 3 -0.032304685640472286 2109 4 -0.11693884561265122 2109 5 -0.06315431661509052 2109 6 -0.014882098576158853 2109 7 -0.0015315285448167515 2109 8 -0.044494880846592366 2109 9 -0.09835385527409812 2109 10-11 -0.030377845572722606 2109 12-13 -0.06977545820514308 2109 14-15 -0.01671991422490038 2109 16-17 -0.07922859175150876 2109 18-19 -0.020378863935675895 2109 20-21 -0.291202567240191 2109 22-23 0.08506867785142447 2109 24-25 -0.40649951382841465 2109 26-27 -0.09834102372898457 2109 28-29 -0.09182294941857805 2109 30-31 0.16165507741284557 2109 32-33 -0.14785627392286216 2109 34-35 -0.08564060451914202 2109 36-37 -0.301338916340832 2109 38-39 -0.34668504550361945 2109 40-41 -0.3971010319427535 2109 42-43 -0.2099331344529034 2109 44-45 -0.23621875217366295 2109 46-47 -0.8544911074524748 2109 48-49 -0.6137130039196421 2109 50-51 -0.5890012770843356 2109 52-53 -0.4770787542019761 2109 54-55 -0.42615885460027414 2109 56-57 -0.417851292290905 2109 58-59 -0.3907203787244953 2109 60-61 -0.47642712391155584 2109 62-63 -0.5250532488368478 2109 64-65 -0.2545284529903995 2109 66-67 -0.38922292763942323 2109 68-69 -0.28487896174005556 2109 70-71 -0.5149225573062992 2109 72-73 -0.515449024109131 2109 74-75 -0.21924227317987288 2109 76-77 -2.878742475609684 2109 78-79 -1.8141491748916678 2109 80-81 -0.5790821009142562 2109 82-83 -0.47168400295081625 2109 84-85 -0.6884072867114455 2109 86-87 -0.3025149521618644 2109 88-89 -0.4987882724709678 2109 90-91 -0.35205129715456707 2109 92-93 -0.5706242279944291 2109 94-95 -0.4660479016517982 2109 96-97 -0.32984835435099313 2109 98-99 -0.10208048153454996 2109 100-101 -0.29306266186013374 2108 1 -0.8304848528788291 2108 2 -0.6062036526451351 2108 3 -0.36582306070310366 2108 4 -0.3165162577931966 2108 5 -0.18310006315325467 2108 6 -0.03486408275612973 2108 7 -0.10453860802719106 2108 8 -0.07404229204468038 2108 9 -0.0656262968313186 2108 10-11 -0.05962544647189816 2108 12-13 -0.0837205910759451 2108 14-15 -0.12889049918621964 2108 16-17 -0.05287841595475129 2108 18-19 -0.031600731286197004 2108 20-21 -0.14498270424458326 2108 22-23 -0.1555372710866294 2108 24-25 -0.4683286847942085 2108 26-27 -0.46446916595537147 2108 28-29 -0.15705248952709638 2108 30-31 -0.36940150740710465 2108 32-33 -0.21429646502920008 2108 34-35 -0.18393577048780685 2108 36-37 -0.3587790680152807 2108 38-39 -0.22421936956357058 2108 40-41 -0.3121471089936563 2108 42-43 -0.2456480587608425 2108 44-45 -0.39262970124897834 2108 46-47 -0.6379957096706192 2108 48-49 -0.6165887792714742 2108 50-51 -0.643029339447871 2108 52-53 -0.5281153154909788 2108 54-55 -0.5607709284514826 2108 56-57 -0.5800650755484966 2108 58-59 -0.4954446309753777 2108 60-61 -0.438495368348498 2108 62-63 -0.6391550798090435 2108 64-65 -0.26635518586231655 2108 66-67 -0.6499234246467935 2108 68-69 -0.34980200524303484 2108 70-71 -0.5052461024976296 2108 72-73 -0.4409813649898524 2108 74-75 -0.22682416816400064 2108 76-77 -0.3725611844770995 2108 78-79 -0.18665551287717363 2108 80-81 -0.2745052211343335 2108 82-83 -0.6425139828399224 2108 84-85 -0.4253523027613966 2108 86-87 -0.583735433674029 2108 88-89 -0.36564674936975194 2108 90-91 -0.44625074447390034 2108 92-93 -0.22360758535297975 2108 94-95 -0.3359210872689218 2108 96-97 -0.36638273885071726 2108 98-99 -0.6122707494212491 2108 100-101 -0.5279964298954667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 15.0 15 220.0 16 334.0 17 2074.0 18 9152.0 19 23789.0 20 35457.0 21 31079.0 22 24243.0 23 25395.0 24 34280.0 25 47363.0 26 67871.0 27 102228.0 28 148247.0 29 205901.0 30 275388.0 31 349435.0 32 408978.0 33 510546.0 34 694840.0 35 1033756.0 36 1808922.0 37 5175279.0 38 5139917.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 71.40818197344191 20.016207039074487 5.176391601978098 3.3992193855054893 2 91.9495981017052 2.3586930597140436 1.5478273239090843 4.1438815146716665 3 6.181720760182062 34.73195957909239 44.4005150448702 14.685804615855352 4 1.7539963115398733 24.506012457420308 72.35245153595771 1.3875396950820966 5 1.2761604062320158 95.39704491117729 1.5956214376873021 1.73117324490339 6 1.1914730249860892 1.8404416941215098 1.435191435512704 95.53289384537969 7 1.3914085360497672 1.3891058019058096 1.5260380115791625 95.69344765046526 8 1.1785727616634878 1.6718840308420289 95.61853450904006 1.531008698454426 9 1.1654806038288898 1.628193983562316 95.84206685493375 1.3642585576750408 10-11 48.27838743489592 1.5626651027882954 48.919259393654194 1.2396880686615896 12-13 1.3054955059852829 48.37641767487115 48.85715366336837 1.4609331557751986 14-15 48.39582997131053 1.4667983186821874 1.811474908028365 48.325896801978914 16-17 1.3070182817901579 47.89463245670349 1.4127831086279548 49.3855661528784 18-19 1.2651759929566047 1.4022227203226008 95.88152284265846 1.4510784440623474 20-21 1.4555662995848455 27.110754517459895 35.129595958676816 36.30408322427844 22-23 10.310377611877751 8.605989126761738 15.02862416153705 66.05500909982347 24-25 1.1223507647212958 1.8034524793978028 39.75707021401623 57.317126541864674 26-27 36.64702347779833 25.385545477792267 6.182903077981782 31.78452796642762 28-29 1.1522120268461662 1.7720777266863799 52.281765025912875 44.793945220554576 30-31 8.115940064287138 41.78684369987723 33.57775742045245 16.51945881538318 32-33 36.59108003740581 47.605029592300305 9.079931430519734 6.723958939774155 34-35 19.72099280773179 35.084352184864485 33.58057084160414 11.614084165799582 36-37 6.721990473489805 32.07322397450799 26.12740656609785 35.07737898590435 38-39 11.957284405432496 52.97413280548724 8.819567718613811 26.249015070466452 40-41 34.138590797271554 14.894731313327897 9.892734681881302 41.07394320751924 42-43 25.72650178966393 6.980463095930728 65.19707659234221 2.0959585220631336 44-45 57.75649069259 16.06462858600548 20.57540312239608 5.603477599008437 46-47 5.9245233077240815 44.39339947256246 37.1360233106025 12.54605390911096 48-49 26.515228469915492 37.273967609073 29.01607822214563 7.194725698865885 50-51 26.215823510036607 21.659746393450973 26.187769770411833 25.93666032610058 52-53 8.37818248536696 1.9643838833618112 58.195994121590175 31.46143950968105 54-55 2.331310950881257 8.998113181735432 43.60327072434421 45.0673051430391 56-57 15.585034679361911 15.125558745750233 16.99297709417112 52.29642948071673 58-59 19.989236574920664 7.936138001619218 33.68740965869456 38.38721576476555 60-61 20.72116557469404 43.449074198736724 20.37070119926023 15.459059027309003 62-63 42.80940003314203 20.027748565449244 18.30205669442885 18.860794706979867 64-65 12.978896741501192 21.61216583969417 49.678183618163594 15.73075380064104 66-67 16.57717263740251 34.088661083279185 18.88274496309404 30.451421316224263 68-69 26.59089371402481 30.54028085556973 10.818619512118726 32.050205918286736 70-71 13.663607311032344 31.668961044114134 28.26275607935742 26.404675565496106 72-73 17.025722964121485 26.174649756922268 18.356508309744235 38.44311896921201 74-75 17.403204229800735 48.46802563884005 23.836619403048363 10.29215072831086 76-77 13.818583794978915 18.831177955604154 33.44220251816359 33.90803573125334 78-79 12.457206750056592 19.311725144662155 31.420340038313288 36.81072806696797 80-81 27.181777152408003 20.5674302149299 33.9185249328849 18.332267699777198 82-83 25.064592621259845 15.213932358670156 35.65626901728778 24.065206002782222 84-85 27.8290621019543 22.98965892855142 23.165793329981987 26.015485639512292 86-87 5.733291141301276 51.20948944360434 18.61781601884627 24.439403396248117 88-89 8.372732062211705 18.00671556510241 7.498213059733852 66.12233931295202 90-91 9.621674398467963 35.71535086147327 13.31176562821404 41.351209111844724 92-93 11.335016929119552 17.41860531192484 33.72833580598697 37.51804195296863 94-95 12.82926854330833 14.112222634279577 17.73123242269483 55.32727639971726 96-97 17.95204419962006 28.22148018884153 20.33732393446394 33.48915167707447 98-99 36.137676636576984 16.391508506900372 33.44366029744021 14.027154559082433 100-101 31.838942440869715 19.834711971599116 34.603083843850115 13.723261743681054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 1.5 6 3.0 7 2.0 8 0.5 9 1.0 10 3.5 11 6.5 12 13.5 13 19.0 14 28.5 15 42.0 16 71.5 17 94.5 18 109.0 19 139.5 20 174.0 21 238.5 22 403.0 23 1083.5 24 1647.5 25 3874.5 26 5706.0 27 11631.5 28 14278.0 29 18236.5 30 23357.5 31 18324.5 32 25260.5 33 91465.0 34 162690.5 35 161423.0 36 145430.5 37 535352.0 38 746511.5 39 405908.0 40 240230.0 41 221296.5 42 237262.0 43 526836.5 44 792566.0 45 742223.5 46 690645.5 47 628766.0 48 432180.5 49 463807.0 50 1507551.0 51 2906897.0 52 2131202.0 53 597328.5 54 533358.5 55 517481.5 56 223777.0 57 125403.5 58 92292.5 59 47132.5 60 28984.0 61 23338.5 62 21279.0 63 20839.0 64 14942.5 65 4060.0 66 2080.0 67 1437.0 68 1114.5 69 775.5 70 598.0 71 464.5 72 365.0 73 302.5 74 208.5 75 151.0 76 112.0 77 90.0 78 60.5 79 42.0 80 37.0 81 34.0 82 32.0 83 27.0 84 25.0 85 25.5 86 23.5 87 27.0 88 32.0 89 27.5 90 26.0 91 29.0 92 28.5 93 31.5 94 28.0 95 30.0 96 44.5 97 49.5 98 53.0 99 78.5 100 526.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6154709E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 4.0867310199490445 #Duplication Level Percentage of deduplicated Percentage of total 1 83.768951824356 3.423411739292128 2 7.1508875350742755 0.5844750781951 3 2.2075297736094592 0.2706474120981261 4 1.0354824584867712 0.1692695313484395 5 0.7654912412393202 0.1564178400536014 6 0.5449303354886073 0.13361902234515188 7 0.39863767268419326 0.11403874598751508 8 0.26843103227558035 0.0877604341054046 9 0.20297187974498776 0.0746542329418088 >10 2.1558389682714005 2.16215240411574 >50 0.9886622221727454 2.792338666480691 >100 0.3493676859385927 2.81072987259577 >500 0.06605723526534885 1.947082551678301 >1k 0.07299897646404993 5.9715872195676605 >5k 0.010442802335406664 2.853762628067638 >10k+ 0.013318356593408652 76.44805262112688 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 2481793 15.362659890685743 No Hit AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 1128198 6.983709827270798 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC 953420 5.901808568634694 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT 914543 5.6611542801544745 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA 761612 4.714489131311496 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT 705504 4.36717244488898 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA 664613 4.1140512032745375 No Hit TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG 425649 2.634829262476966 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 394269 2.440582495172151 No Hit AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA 308012 1.906639110614744 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 278782 1.7257011562387163 No Hit AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG 227502 1.4082704925232636 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT 225823 1.397877238147713 No Hit CGTTACCTTGTTACGACTTCTCCTTCCTCTAGATAACGTGATTTAATCAC 169934 1.0519161936002683 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA 163688 1.0132525445057539 No Hit AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG 153981 0.9531648016686652 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGTTAAGCTTTACATAT 143525 0.8884406398159199 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT 143412 0.887741153368965 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA 142324 0.8810062750124437 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC 117162 0.7252498327267919 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA 108415 0.6711046296160458 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC 84349 0.5221325868513014 No Hit GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA 80690 0.49948284429016954 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC 71952 0.44539335248935774 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA 71066 0.43990888353358765 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGTCAAGGTTTACTTAA 62767 0.38853686562846784 No Hit GGAAACCTTGTTACGCCTTATCCTTCCTCTAAGTGATATGGCTTACACAA 57257 0.35442916365748217 No Hit AGCAACCTTGTTACGACTTTTGCTTCTTCTAGCATGATAACATTGGATGG 52959 0.3278239180910037 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA 49123 0.30407851976782746 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT 42205 0.26125509286487303 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 42148 0.26090225456862143 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT 39182 0.24254228287244295 No Hit CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG 37999 0.2352193406888357 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA 35509 0.21980587827363524 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA 35288 0.21843785610746688 No Hit GGAAACCTTGTTACGCCTTTTCCTTCCTCTAAGTGATATGACTTACAAAA 33134 0.20510428259648628 No Hit TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC 32456 0.200907363914757 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC 30730 0.19022317269843733 No Hit GGAAACCTTGTTACGACTTCATCTTCCTCTAAATGTTAAGGTTTATTCAA 30213 0.18702286744998006 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 30092 0.186273859838639 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 29012 0.1795885026465039 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTACTTAA 28190 0.17450020300582325 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA 26882 0.16640349262868184 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTAAATCCACTGTGGAAGGT 26725 0.16543163977760292 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG 25210 0.15605356927196895 No Hit AGCAACCTTGTTACGACTTCTACCATGTCAAGATCCCAAGTATCGTTACA 25183 0.15588643534216556 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC 24296 0.15039577624084716 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAGATATCGGTAAA 23975 0.14840873951985145 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC 22968 0.14217526295274027 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCCAAGGTTTGTTTAA 22746 0.1408010506410236 No Hit AGCAACCTTGTTACGACTTCTACCATGTCAAAATCCCAGGTATCGCTACA 22185 0.13732837898844233 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAGATAA 21966 0.13597273711337046 No Hit GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTGACTAG 21762 0.13470994742152273 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC 21647 0.1339980806834713 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCTAT 21385 0.1323762625498237 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA 21301 0.13185629032376875 No Hit GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT 19360 0.11984121781457036 No Hit GGAAACCTTGTTACGCCTTACCCTTCCTCTAAGTGATATGACTTACACAA 19090 0.11816987851653658 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATAACATGGTTTACTCAA 18596 0.11511194661568958 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCGACCAGGTTTGCAATC 17336 0.10731236322486527 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACTCCGTGGTAACC 16943 0.10487963602439387 No Hit GGTTACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 16303 0.10091794287349898 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGC 16298 0.10088699214575762 No Hit AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 16275 0.10074461879814735 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.9712698631711656E-4 0.0 0.0 2 3.0331713186538986E-4 0.0 0.0 3 3.095072774136631E-4 0.0 0.0 4 3.095072774136631E-4 0.0 0.0 5 3.1569742296193635E-4 0.0 0.0 6 3.1569742296193635E-4 0.0 0.0 7 3.280777140584829E-4 0.0 0.0 8 3.280777140584829E-4 0.0 0.0 9 3.404580051550294E-4 0.0 0.0 10-11 3.4355307792916607E-4 0.0 0.0 12-13 3.4974322347743926E-4 0.0 0.0 14-15 3.7140873289639575E-4 0.0 3.095072774136631E-6 16-17 3.899791695412155E-4 0.0 6.190145548273262E-6 18-19 4.116446789601719E-4 0.0 6.190145548273262E-6 20-21 4.271200428308551E-4 0.0 6.190145548273262E-6 22-23 4.395003339274016E-4 0.0 6.190145548273262E-6 24-25 4.6735598889463125E-4 0.0 6.190145548273262E-6 26-27 4.921165710877243E-4 0.0 6.190145548273262E-6 28-29 4.983067166359975E-4 0.0 6.190145548273262E-6 30-31 5.261623716032273E-4 0.0 6.190145548273262E-6 32-33 5.540180265704569E-4 0.0 6.190145548273262E-6 34-35 6.03539190956643E-4 0.0 6.190145548273262E-6 36-37 6.282997731497361E-4 0.0 6.190145548273262E-6 38-39 6.71630791987649E-4 0.0 6.190145548273262E-6 40-41 7.366273202445182E-4 0.0 6.190145548273262E-6 42-43 8.294795034686171E-4 0.0 6.190145548273262E-6 44-45 9.161415411444427E-4 0.0 6.190145548273262E-6 46-47 0.0010523247432064546 2.4760582193093048E-5 6.190145548273262E-6 48-49 0.0011544621447529633 2.4760582193093048E-5 6.190145548273262E-6 50-51 0.001265884764621882 2.4760582193093048E-5 6.190145548273262E-6 52-53 0.0013927827483614839 2.4760582193093048E-5 6.190145548273262E-6 54-55 0.0016032476970027749 2.4760582193093048E-5 6.190145548273262E-6 56-57 0.0018137126456440659 2.4760582193093048E-5 6.190145548273262E-6 58-59 0.002055128322026723 2.4760582193093048E-5 6.190145548273262E-6 60-61 0.002358445453892113 2.4760582193093048E-5 6.190145548273262E-6 62-63 0.0026462872218868195 2.4760582193093048E-5 6.190145548273262E-6 64-65 0.0028753226071729305 2.4760582193093048E-5 6.190145548273262E-6 66-67 0.003231255976198643 2.4760582193093048E-5 6.190145548273262E-6 68-69 0.003611949927417448 2.4760582193093048E-5 6.190145548273262E-6 70-71 0.00403287982470003 2.4760582193093048E-5 6.190145548273262E-6 72-73 0.004441429430886066 2.4760582193093048E-5 6.190145548273262E-6 74-75 0.005017112966875479 2.4760582193093048E-5 6.190145548273262E-6 76-77 0.005571130993445935 2.4760582193093048E-5 6.190145548273262E-6 78-79 0.0061560997477577584 2.4760582193093048E-5 6.190145548273262E-6 80-81 0.006741068502069582 2.4760582193093048E-5 6.190145548273262E-6 82-83 0.007421984512379641 2.4760582193093048E-5 6.190145548273262E-6 84-85 0.008288604889137897 2.4760582193093048E-5 6.190145548273262E-6 86-87 0.0091676055569927 2.4760582193093048E-5 6.190145548273262E-6 88-89 0.010046606224847505 2.4760582193093048E-5 6.190145548273262E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTTGT 1498370 0.0 94.96291 5 TGTTACG 1499385 0.0 94.9604 9 CTTGTTA 1497940 0.0 94.95719 7 CCTTGTT 1501240 0.0 94.93798 6 TTGTTAC 1498875 0.0 94.924255 8 GTTACCT 682065 0.0 94.90041 2 GATACCT 169525 0.0 94.843094 2 TACCTTG 1129380 0.0 94.81494 4 AGATACC 149800 0.0 94.76536 1 TCGTACC 49400 0.0 94.75 1 AAACCTT 349290 0.0 94.69537 3 AACCTTG 368285 0.0 94.477646 4 AGGTACC 26520 0.0 94.46267 1 GGAAACC 346635 0.0 94.40391 1 CTACCTT 199225 0.0 94.34671 3 GGTTACC 657580 0.0 94.34339 1 TTACCTT 687845 0.0 94.130585 3 GAAACCT 351720 0.0 94.12758 2 ATACCTT 173515 0.0 94.069244 3 GGTACCT 28535 0.0 93.85141 2 >>END_MODULE