FastQCFastQC Report
Thu 16 Jul 2015
000000000-AGRGJ l01n03.3330000000e383.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGRGJ l01n03.3330000000e383.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16154709
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC248179315.362659890685743No Hit
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT11281986.983709827270798No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC9534205.901808568634694No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT9145435.6611542801544745No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA7616124.714489131311496No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT7055044.36717244488898No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA6646134.1140512032745375No Hit
TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG4256492.634829262476966No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC3942692.440582495172151No Hit
AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA3080121.906639110614744No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC2787821.7257011562387163No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG2275021.4082704925232636No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT2258231.397877238147713No Hit
CGTTACCTTGTTACGACTTCTCCTTCCTCTAGATAACGTGATTTAATCAC1699341.0519161936002683No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA1636881.0132525445057539No Hit
AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG1539810.9531648016686652No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGTTAAGCTTTACATAT1435250.8884406398159199No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT1434120.887741153368965No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA1423240.8810062750124437No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC1171620.7252498327267919No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA1084150.6711046296160458No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC843490.5221325868513014No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA806900.49948284429016954No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC719520.44539335248935774No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA710660.43990888353358765No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGTCAAGGTTTACTTAA627670.38853686562846784No Hit
GGAAACCTTGTTACGCCTTATCCTTCCTCTAAGTGATATGGCTTACACAA572570.35442916365748217No Hit
AGCAACCTTGTTACGACTTTTGCTTCTTCTAGCATGATAACATTGGATGG529590.3278239180910037No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA491230.30407851976782746No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT422050.26125509286487303No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC421480.26090225456862143No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT391820.24254228287244295No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG379990.2352193406888357No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA355090.21980587827363524No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA352880.21843785610746688No Hit
GGAAACCTTGTTACGCCTTTTCCTTCCTCTAAGTGATATGACTTACAAAA331340.20510428259648628No Hit
TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC324560.200907363914757No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC307300.19022317269843733No Hit
GGAAACCTTGTTACGACTTCATCTTCCTCTAAATGTTAAGGTTTATTCAA302130.18702286744998006No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC300920.186273859838639No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC290120.1795885026465039No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTACTTAA281900.17450020300582325No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA268820.16640349262868184No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTAAATCCACTGTGGAAGGT267250.16543163977760292No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG252100.15605356927196895No Hit
AGCAACCTTGTTACGACTTCTACCATGTCAAGATCCCAAGTATCGTTACA251830.15588643534216556No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC242960.15039577624084716No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAGATATCGGTAAA239750.14840873951985145No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC229680.14217526295274027No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCCAAGGTTTGTTTAA227460.1408010506410236No Hit
AGCAACCTTGTTACGACTTCTACCATGTCAAAATCCCAGGTATCGCTACA221850.13732837898844233No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAGATAA219660.13597273711337046No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTGACTAG217620.13470994742152273No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC216470.1339980806834713No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCTAT213850.1323762625498237No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA213010.13185629032376875No Hit
GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT193600.11984121781457036No Hit
GGAAACCTTGTTACGCCTTACCCTTCCTCTAAGTGATATGACTTACACAA190900.11816987851653658No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATAACATGGTTTACTCAA185960.11511194661568958No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCGACCAGGTTTGCAATC173360.10731236322486527No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACTCCGTGGTAACC169430.10487963602439387No Hit
GGTTACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT163030.10091794287349898No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGC162980.10088699214575762No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC162750.10074461879814735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTGT14983700.094.962915
TGTTACG14993850.094.96049
CTTGTTA14979400.094.957197
CCTTGTT15012400.094.937986
TTGTTAC14988750.094.9242558
GTTACCT6820650.094.900412
GATACCT1695250.094.8430942
TACCTTG11293800.094.814944
AGATACC1498000.094.765361
TCGTACC494000.094.751
AAACCTT3492900.094.695373
AACCTTG3682850.094.4776464
AGGTACC265200.094.462671
GGAAACC3466350.094.403911
CTACCTT1992250.094.346713
GGTTACC6575800.094.343391
TTACCTT6878450.094.1305853
GAAACCT3517200.094.127582
ATACCTT1735150.094.0692443
GGTACCT285350.093.851412
Produced by FastQC (version 0.11.2)