##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AGRGJ l01n02.3320000000e386.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16154709
Sequences flagged as poor quality	0
Sequence length	12
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046734856071996	33.0	32.0	34.0	16.0	35.0
2	31.41731980439883	33.0	32.0	34.0	18.0	35.0
3	31.461918688847938	33.0	32.0	34.0	18.0	35.0
4	31.50828609788019	33.0	32.0	34.0	18.0	35.0
5	31.536358779350344	33.0	32.0	34.0	18.0	35.0
6	34.099267464365965	37.0	34.0	37.0	29.0	37.0
7	33.54908479007576	37.0	33.0	37.0	18.0	37.0
8	33.56533057946138	37.0	33.0	37.0	18.0	37.0
9	33.64194396816433	37.0	34.0	37.0	18.0	37.0
10	33.39726961346069	37.0	33.0	37.0	18.0	37.0
11	33.262075472854384	35.0	33.0	37.0	18.0	37.0
12	33.0004865454401	35.0	33.0	37.0	18.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.07587036113330825
1101	2	0.005300957454544175
1101	3	0.14825148643013364
1101	4	0.20043408912845706
1101	5	0.2314347424494656
1101	6	0.1947741287367606
1101	7	0.2332193017367743
1101	8	0.2296913220481045
1101	9	0.2353664694753448
1101	10	0.24270019741915405
1101	11	0.2891041573719306
1101	12	0.2828571373404358
1102	1	0.4498918271797585
1102	2	0.41520432943179486
1102	3	0.3310600718474035
1102	4	0.32062005793299164
1102	5	0.38926653911278564
1102	6	0.2688752425527525
1102	7	0.38543007165661436
1102	8	0.2646890780247091
1102	9	0.2371200768103634
1102	10	0.19670547576659203
1102	11	0.3107355553236246
1102	12	0.24747185596127252
1103	1	0.41683296580259466
1103	2	0.23144310821611214
1103	3	0.4112538688137519
1103	4	0.11390675607521317
1103	5	0.28970309181610787
1103	6	0.21136819775593096
1103	7	0.26761864531705726
1103	8	0.21770318787826426
1103	9	0.16901065409368243
1103	10	0.2829056412615927
1103	11	0.2735914514615203
1103	12	0.25577980782442467
2112	1	0.41265059084530975
2112	2	0.3795895324914227
2112	3	0.3480052889687819
2112	4	0.301587079449984
2112	5	0.18749120491387217
2112	6	0.1374215602558948
2112	7	0.15867170883536375
2112	8	0.12752940015380432
2112	9	0.1842623897612654
2112	10	0.21730248183985879
2112	11	0.26354847854464936
2112	12	0.03298982518077054
1113	1	0.27116501451407515
1113	2	0.23242770328365836
1113	3	0.2967020096874755
1113	4	0.3003008200392081
1113	5	0.19154553167478028
1113	6	0.08933000974090533
1113	7	0.1336748768567233
1113	8	0.13443590185347887
1113	9	0.13914109783880235
1113	10	0.10887073160951388
1113	11	0.14183574128840348
1113	12	0.15173998475268746
2113	1	0.3138962064628217
2113	2	0.1893177398331467
2113	3	0.1789947911873746
2113	4	0.18158446154446395
2113	5	0.08054141039423968
2113	6	-0.14342026879175052
2113	7	-0.025444678251609787
2113	8	0.04240098043548812
2113	9	0.061752027418151556
2113	10	0.12006597919314999
2113	11	0.03698810310566358
2113	12	0.13673747187965546
1112	1	0.28053375283869286
1112	2	0.13641040855981856
1112	3	0.2892026504047642
1112	4	0.1852360045104362
1112	5	0.14388485286739439
1112	6	0.162847776516017
1112	7	0.08316244037741427
1112	8	0.1389227255108949
1112	9	0.10874131891161198
1112	10	0.12610386488528746
1112	11	0.14847620114044702
1112	12	0.19119176095356494
2114	1	0.3146337842896969
2114	2	0.21469351524311975
2114	3	0.27780422475574795
2114	4	0.14467770941147506
2114	5	-0.012459678950961006
2114	6	0.1266953893457341
2114	7	0.00834778946546777
2114	8	0.04553224563337466
2114	9	0.17815540421985787
2114	10	0.10925453894146386
2114	11	0.048808736990416435
2114	12	0.17169040124993984
1114	1	0.3164444554613688
1114	2	0.12897096160698496
1114	3	0.1421208821288964
1114	4	0.20899518861011046
1114	5	0.2033443178929879
1114	6	0.1066455111761968
1114	7	0.029512734083972703
1114	8	0.14835043296429262
1114	9	0.21294517629272036
1114	10	0.1399760938078103
1114	11	0.14834245093873477
1114	12	-0.011027282556085538
1109	1	0.3045290710477957
1109	2	0.15562249130528727
1109	3	0.10867837486421905
1109	4	0.15803659574617868
1109	5	0.15252444287365918
1109	6	0.11194450443984749
1109	7	0.10602734131586544
1109	8	0.12068810999934954
1109	9	0.17795134936745427
1109	10	0.07157224694261544
1109	11	0.11366127413997162
1109	12	-0.019318827322294396
1108	1	0.2821095091290893
1108	2	0.20474296694255045
1108	3	-0.05597709094377379
1108	4	0.04402908956918594
1108	5	0.057469606280715624
1108	6	0.05560581016056432
1108	7	0.07544193030702218
1108	8	0.060697407939386494
1108	9	0.1730130207581766
1108	10	0.15218530559997134
1108	11	7.243421592093569E-4
1108	12	-0.19034183382217407
1111	1	0.29240116172377384
1111	2	0.024909708023773902
1111	3	0.025378169879253676
1111	4	0.04602136047252259
1111	5	0.026734788758567163
1111	6	0.01148282627035968
1111	7	0.0028641814046324043
1111	8	-0.034891797942798064
1111	9	0.020829490130857664
1111	10	0.11632669755289271
1111	11	0.0247007150600993
1111	12	-0.18880090629241408
1110	1	0.21083771563298725
1110	2	0.07952511241382965
1110	3	-0.013165014879099601
1110	4	0.02391006252697636
1110	5	0.06113896424067988
1110	6	-0.07376035816108129
1110	7	0.01980402953380178
1110	8	0.017413907867862122
1110	9	0.028225771016124668
1110	10	0.13113270427001567
1110	11	-0.046584437873192996
1110	12	-0.07028563822413503
1105	1	0.2885340913372829
1105	2	0.15306326290786032
1105	3	0.10425450421739058
1105	4	0.06754701893303405
1105	5	0.10959864883273696
1105	6	0.03381817172871848
1105	7	-0.07737176342311614
1105	8	0.06036637821087254
1105	9	0.14852043909304768
1105	10	0.17950530598914582
1105	11	-0.024810369011667888
1105	12	0.15592453209679036
1104	1	-1.504596514451496
1104	2	-0.7199801899890588
1104	3	-0.4484369400916961
1104	4	-0.19318664936197294
1104	5	-0.060285426594305136
1104	6	0.11441180419044628
1104	7	0.08508787499646786
1104	8	0.16431474688992154
1104	9	0.14165838562519184
1104	10	0.11806861331314167
1104	11	0.0968717745530796
1104	12	0.07460547385596072
1107	1	-0.6035761291012065
1107	2	-0.26731163386023127
1107	3	-0.2566602318053164
1107	4	-0.03389061606283761
1107	5	-0.09825588113590555
1107	6	0.03541569212190865
1107	7	-0.005319395301526697
1107	8	-0.002561601868549701
1107	9	-0.01767423969200621
1107	10	-0.07673655097501353
1107	11	0.10856363175228978
1107	12	0.017331702825877926
1106	1	-0.37458200795801844
1106	2	-0.15082407250620022
1106	3	-0.18709460083765705
1106	4	0.006152087259010841
1106	5	-0.23893475511231443
1106	6	-0.06486077293002523
1106	7	-0.14918699327789398
1106	8	-0.03761267778124022
1106	9	-0.0740926092032268
1106	10	-0.06109459912442361
1106	11	0.04055432282517302
1106	12	-0.1808413532599431
2103	1	-0.010748235576748755
2103	2	0.02401535296848678
2103	3	0.156976224139175
2103	4	0.020356821996280416
2103	5	0.0353075643559535
2103	6	0.06971964372652906
2103	7	0.08644711562783414
2103	8	0.1498465668280886
2103	9	0.01357003251573019
2103	10	0.14889097646612726
2103	11	0.17696845558759833
2103	12	0.11178106804187138
2102	1	0.09997184699633976
2102	2	0.07138728180257559
2102	3	0.10025412309614978
2102	4	-0.29251112158128123
2102	5	-0.10395120687321935
2102	6	0.03594630399322796
2102	7	0.06095841849567307
2102	8	-0.20190709413887475
2102	9	-0.12139574859266844
2102	10	0.02709843984330007
2102	11	0.04775046496878588
2102	12	-0.08124512959302876
2101	1	0.015062752253747647
2101	2	0.04584404208068804
2101	3	-0.24939129985299502
2101	4	-0.1755248874927986
2101	5	-0.04151504436783782
2101	6	0.01928008945409232
2101	7	-0.13890182260819017
2101	8	-0.1966378244578877
2101	9	-0.08452201255313696
2101	10	0.06569458198151779
2101	11	-0.2496495951720732
2101	12	-0.010813876974644643
2107	1	-0.23628470226870846
2107	2	-0.07609104829283808
2107	3	-0.013604867838417078
2107	4	-0.056515076932353026
2107	5	-0.02292767931320938
2107	6	-0.11682046103420163
2107	7	-0.009235829762737069
2107	8	-0.06411999934576329
2107	9	0.01877052132656587
2107	10	-0.30240463804516793
2107	11	-0.17027971337733305
2107	12	0.12923508529229366
2106	1	-0.08704456538559668
2106	2	-0.20134343909271735
2106	3	-0.08555763066651068
2106	4	-0.14681537336304373
2106	5	-0.1464781512236506
2106	6	-0.24596122416954813
2106	7	-0.18515256099827582
2106	8	-0.2547416382250347
2106	9	-0.22608457879187682
2106	10	-0.4915791297844123
2106	11	-0.3592089144078514
2106	12	-0.04256296745680288
2105	1	-0.39884994980456767
2105	2	-0.22344267276527674
2105	3	-0.3205989142218719
2105	4	-0.4429586187084844
2105	5	-0.43429739820816593
2105	6	-0.4468518745710881
2105	7	-0.48161613603588904
2105	8	-0.2806284033749975
2105	9	-0.36382943583844707
2105	10	-0.7349304470294271
2105	11	-0.6123114684755109
2105	12	-0.3168675645271577
2104	1	-0.16567848604119106
2104	2	-0.41423737782900005
2104	3	-0.24686036216099794
2104	4	-0.20851864876756565
2104	5	-0.22269000967258634
2104	6	-0.3150479819703662
2104	7	-0.33173850837107466
2104	8	-0.18637112473943773
2104	9	-0.3696550399703895
2104	10	-0.6210836923451382
2104	11	-0.3090689128030846
2104	12	-0.12263851713919394
2111	1	-0.4732694070864021
2111	2	-0.2186782170730588
2111	3	-0.4119847035254196
2111	4	-0.28988065135250096
2111	5	-0.14981709030611157
2111	6	-0.10929402246092224
2111	7	-0.11206073185704213
2111	8	-0.14400696330706353
2111	9	-0.34983561360305515
2111	10	-0.15543349183720068
2111	11	-0.12160361482692394
2111	12	-0.05094244163789341
2110	1	-0.17782223999410007
2110	2	-0.09870079796738551
2110	3	0.00934121478488592
2110	4	-0.013403834794424085
2110	5	-0.012383018173327542
2110	6	0.0387976472802265
2110	7	0.1277155864988302
2110	8	0.10030864575723086
2110	9	-0.1394107270545959
2110	10	0.12688253976079267
2110	11	0.05830795132450817
2110	12	0.10421357576207413
2109	1	-0.07096194521072263
2109	2	-0.07849619025128263
2109	3	-0.39753451457652744
2109	4	-0.2048617579400407
2109	5	-0.259102204721124
2109	6	-0.12913806687529217
2109	7	-0.07946281158168489
2109	8	-0.2647639900741652
2109	9	-0.2713851682614674
2109	10	-0.1271147984219354
2109	11	-0.09498365225394423
2109	12	-0.3896534051823224
2108	1	-0.09021020150329662
2108	2	-0.24336283493892452
2108	3	-0.24141171380508197
2108	4	-0.26532796684835347
2108	5	-0.35688816181138705
2108	6	-0.1792252784817947
2108	7	-0.26849281504051703
2108	8	-0.3546479227390975
2108	9	-0.2311484510943842
2108	10	-0.11086506888118208
2108	11	-0.341033130334381
2108	12	-0.38820993902949397
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	55.0
15	2239.0
16	70949.0
17	152397.0
18	213501.0
19	247239.0
20	232579.0
21	259656.0
22	252124.0
23	217182.0
24	209715.0
25	209723.0
26	197723.0
27	201412.0
28	220170.0
29	237747.0
30	292997.0
31	367029.0
32	578023.0
33	1348363.0
34	3929651.0
35	5727493.0
36	986742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.434021002792438	41.41167135848748	33.95446491793817	11.19984272078191
2	31.32989891678024	38.005079509634	20.423865264301572	10.241156309284184
3	15.192796106695578	17.458587462021136	32.93103577415105	34.417580657132234
4	28.47963754464875	15.717010528262755	22.962904867293478	32.84044705979502
5	37.83955501767318	24.596654758683677	12.49172609670654	25.07206412693661
6	17.393454750562203	46.848352390624925	26.14688385906549	9.61130899974738
7	23.680965098164254	13.18557951121249	34.24709166844169	28.886363722181564
8	22.648355968529053	33.44210657090759	21.64631377761122	22.263223682952134
9	11.25128283028806	36.01303496088973	29.07722447987147	23.658457728950737
10	33.20408720504804	25.057724661920517	5.799628554080244	35.9385595789512
11	31.71203290780823	22.818810641381486	1.4132475445082353	44.055908906302044
12	16.0270977335463	32.508310734659474	25.10815267548304	26.356438856311186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	867.0
1	867.0
2	867.0
3	867.0
4	2558.5
5	4250.0
6	4250.0
7	4250.0
8	4250.0
9	4250.0
10	4250.0
11	4250.0
12	4250.0
13	10767.0
14	17284.0
15	17284.0
16	17284.0
17	17284.0
18	17284.0
19	17284.0
20	17284.0
21	38656.0
22	60028.0
23	60028.0
24	60028.0
25	60028.0
26	60028.0
27	60028.0
28	60028.0
29	152942.5
30	245857.0
31	245857.0
32	245857.0
33	245857.0
34	245857.0
35	245857.0
36	245857.0
37	245857.0
38	3183352.5
39	6120848.0
40	6120848.0
41	6120848.0
42	6120848.0
43	6120848.0
44	6120848.0
45	6120848.0
46	5699419.5
47	5277991.0
48	5277991.0
49	5277991.0
50	5277991.0
51	5277991.0
52	5277991.0
53	5277991.0
54	4730807.5
55	4183624.0
56	4183624.0
57	4183624.0
58	4183624.0
59	4183624.0
60	4183624.0
61	4183624.0
62	4183624.0
63	2184607.5
64	185591.0
65	185591.0
66	185591.0
67	185591.0
68	185591.0
69	185591.0
70	185591.0
71	115870.0
72	46149.0
73	46149.0
74	46149.0
75	46149.0
76	46149.0
77	46149.0
78	46149.0
79	28002.5
80	9856.0
81	9856.0
82	9856.0
83	9856.0
84	9856.0
85	9856.0
86	9856.0
87	9856.0
88	5742.0
89	1628.0
90	1628.0
91	1628.0
92	1628.0
93	1628.0
94	1628.0
95	1628.0
96	1182.0
97	736.0
98	736.0
99	736.0
100	736.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	1.2380291096546522E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.2380291096546522E-5
11	1.2380291096546522E-5
12	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	1.6154709E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.7935254459671195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56646549687898	2.055097933353936
2	11.14652417135086	0.6227619781351238
3	4.482257030311253	0.375638972086185
4	2.16745526036486	0.24219365691298098
5	1.5377742637400886	0.21479057679556457
6	1.0645567877421425	0.17843198851408157
7	0.9204197581819774	0.17998512108165798
8	0.6454894287346282	0.1442552915418371
9	0.40332079906980084	0.10140182235803526
>10	3.2915278178785092	1.8090480373306486
>50	0.3874221525726912	0.751027856895388
>100	0.27409557637755194	1.5919188641877036
>500	0.06010597204619808	1.188499568189044
>1k	0.046818068416729405	2.188875093015916
>5k	8.872948205810344E-4	0.15925523236439076
>10k+	0.004880121513195687	88.19681800723748
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCGTTGACCA	1116052	6.908524319441471	No Hit
GAGTGAGTACAA	953130	5.900013426425694	No Hit
TGCCCATTTAAG	916277	5.671887992535179	No Hit
AGCAGTCGCGAT	912883	5.650878638544341	No Hit
AATCAGTCTCGT	882188	5.460872120940093	No Hit
ATCGAATGCGGC	851767	5.272561703216072	No Hit
AGTTCAGACGCT	819109	5.070403929900563	No Hit
CAGGGTGATACC	769944	4.7660654240197085	No Hit
TAATGACCACGC	735385	4.552140184016933	No Hit
TGAGAACCTCCA	731778	4.529812329024311	No Hit
TGCCTACAAGCA	704796	4.362789821840802	No Hit
ATTTCGACATGC	661798	4.096625943556148	No Hit
TTCAGACTACCA	661099	4.092299031817905	No Hit
AATCCGTACAGC	653092	4.0427345364128815	No Hit
CTTATCAAGGCG	608557	3.7670564044205315	No Hit
GGAGACAAGGGA	515333	3.189986275828305	No Hit
AACGGATAAACG	474992	2.9402696142654134	No Hit
ACACCTGGTGAT	468070	2.897421426780266	No Hit
GCGGATGTGACT	440765	2.728399502584664	No Hit
ACCGTGCATAGG	331107	2.049600522052115	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCCG	65	0.0012619785	6.0000005	5
GTTGAAC	160	1.0913936E-11	6.0000005	5
TATCACG	235	0.0	6.0000005	3
AACGTAT	65	0.0012619785	6.0000005	1
GATGTCA	65	0.0012619785	6.0000005	4
GGCAAGG	65	0.0012619785	6.0000005	4
CCGGATG	130	3.6470738E-9	6.0000005	1
TGAGTCT	130	3.6470738E-9	6.0000005	3
AGTGGCG	80	6.5394415E-5	6.0000005	4
CAGGGTC	80	6.5394415E-5	6.0000005	1
AACGGGT	65	0.0012619785	6.0000005	1
AGTTGCG	205	0.0	6.0000005	4
CCTCAGT	80	6.5394415E-5	6.0000005	1
TTGACCG	65	0.0012619785	6.0000005	6
TATTAAG	130	3.6470738E-9	6.0000005	3
CAAGCCG	205	0.0	6.0000005	6
ATGCGGG	80	6.5394415E-5	6.0000005	6
TACTAGC	65	0.0012619785	6.0000005	5
TAAGGCT	65	0.0012619785	6.0000005	6
AGCTCAG	955	0.0	6.0000005	1
>>END_MODULE