FastQCFastQC Report
Thu 16 Jul 2015
000000000-AGRGJ l01n02.3320000000e386.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGRGJ l01n02.3320000000e386.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16154709
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCGTTGACCA11160526.908524319441471No Hit
GAGTGAGTACAA9531305.900013426425694No Hit
TGCCCATTTAAG9162775.671887992535179No Hit
AGCAGTCGCGAT9128835.650878638544341No Hit
AATCAGTCTCGT8821885.460872120940093No Hit
ATCGAATGCGGC8517675.272561703216072No Hit
AGTTCAGACGCT8191095.070403929900563No Hit
CAGGGTGATACC7699444.7660654240197085No Hit
TAATGACCACGC7353854.552140184016933No Hit
TGAGAACCTCCA7317784.529812329024311No Hit
TGCCTACAAGCA7047964.362789821840802No Hit
ATTTCGACATGC6617984.096625943556148No Hit
TTCAGACTACCA6610994.092299031817905No Hit
AATCCGTACAGC6530924.0427345364128815No Hit
CTTATCAAGGCG6085573.7670564044205315No Hit
GGAGACAAGGGA5153333.189986275828305No Hit
AACGGATAAACG4749922.9402696142654134No Hit
ACACCTGGTGAT4680702.897421426780266No Hit
GCGGATGTGACT4407652.728399502584664No Hit
ACCGTGCATAGG3311072.049600522052115No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCG650.00126197856.00000055
GTTGAAC1601.0913936E-116.00000055
TATCACG2350.06.00000053
AACGTAT650.00126197856.00000051
GATGTCA650.00126197856.00000054
GGCAAGG650.00126197856.00000054
CCGGATG1303.6470738E-96.00000051
TGAGTCT1303.6470738E-96.00000053
AGTGGCG806.5394415E-56.00000054
CAGGGTC806.5394415E-56.00000051
AACGGGT650.00126197856.00000051
AGTTGCG2050.06.00000054
CCTCAGT806.5394415E-56.00000051
TTGACCG650.00126197856.00000056
TATTAAG1303.6470738E-96.00000053
CAAGCCG2050.06.00000056
ATGCGGG806.5394415E-56.00000056
TACTAGC650.00126197856.00000055
TAAGGCT650.00126197856.00000056
AGCTCAG9550.06.00000051
Produced by FastQC (version 0.11.2)