##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AGRGJ l01n01.3310000000e389.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16154709 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.968628404262805 33.0 32.0 33.0 31.0 34.0 2 32.68135470592507 33.0 32.0 34.0 32.0 34.0 3 32.426383106003335 33.0 32.0 34.0 32.0 34.0 4 32.388470012056544 33.0 32.0 34.0 32.0 34.0 5 32.50599958191757 33.0 32.0 34.0 32.0 34.0 6 34.15215699645224 34.0 33.0 37.0 32.0 37.0 7 35.9602820453157 37.0 37.0 37.0 34.0 37.0 8 35.97262383370694 37.0 37.0 37.0 33.0 37.0 9 35.820978700390086 37.0 37.0 37.0 33.0 37.0 10-11 34.02386366105388 35.0 33.0 35.5 32.0 37.0 12-13 35.28548787229779 35.5 35.5 36.0 34.0 37.5 14-15 36.94220035161265 38.0 38.0 38.0 35.5 38.0 16-17 37.002008114166586 38.0 38.0 38.0 35.5 38.0 18-19 36.883966866874545 38.0 38.0 38.0 36.0 38.0 20-21 36.91313149001941 38.0 38.0 38.0 36.0 38.0 22-23 36.95552677550552 38.5 38.0 38.5 35.0 38.5 24-25 37.40104167150272 39.0 38.0 39.0 35.0 39.0 26-27 37.68190318377137 39.0 38.0 39.0 36.5 39.0 28-29 37.36848593187287 39.0 38.0 39.0 35.0 39.0 30-31 37.39370028268537 39.0 38.0 39.0 35.0 39.0 32-33 37.262932900865 39.0 38.0 39.0 35.0 39.0 34-35 37.35185691057635 39.0 38.0 39.0 36.0 39.0 36-37 37.10396776568368 38.5 38.0 39.0 34.5 39.0 38-39 37.591270724839426 39.0 38.0 39.0 36.0 39.0 40-41 37.42868519018201 39.0 38.0 39.0 36.0 39.0 42-43 36.5797334696651 39.0 37.0 39.0 33.5 39.0 44-45 36.89085386805792 39.0 37.5 39.0 34.0 39.0 46-47 36.80850889978891 38.5 37.5 39.0 34.0 39.0 48-49 36.85004065997103 38.0 38.0 39.0 34.5 39.0 50-51 36.93631067572929 38.0 38.0 39.0 34.5 39.0 52-53 36.95963551556392 38.5 38.0 39.0 35.0 39.0 54-55 36.58101479884286 38.0 37.5 39.0 33.5 39.0 56-57 36.865619306420186 38.0 37.5 39.0 34.0 39.0 58-59 36.558089594804834 38.5 37.0 39.0 33.0 39.0 60-61 37.06384998949842 39.0 38.0 39.0 34.5 39.0 62-63 36.93343971717472 38.5 38.0 39.0 34.0 39.0 64-65 36.35891550259432 38.0 37.5 39.0 32.0 39.0 66-67 36.62298553319654 38.5 37.0 39.0 33.0 39.0 68-69 36.76081958517483 38.0 37.0 39.0 33.5 39.0 70-71 36.514771482420386 38.5 37.0 39.0 33.0 39.0 72-73 36.56809754357073 38.0 37.0 39.0 33.0 39.0 74-75 37.12762665672282 39.0 38.0 39.0 35.0 39.0 76-77 36.79880962263077 39.0 37.5 39.0 34.0 39.0 78-79 37.03192004263277 39.0 38.0 39.0 34.0 39.0 80-81 37.24015691028542 39.0 38.0 39.0 36.0 39.0 82-83 36.670654791739054 38.0 37.5 39.0 33.5 39.0 84-85 36.697276038831774 38.5 37.0 39.0 33.5 39.0 86-87 37.106804245127535 39.0 38.0 39.0 35.0 39.0 88-89 36.62776658496294 39.0 37.0 39.0 32.5 39.0 90-91 36.3488632323863 38.0 37.0 39.0 33.0 39.0 92-93 36.55777915900559 38.5 37.0 39.0 33.0 39.0 94-95 36.610188521501684 38.5 37.5 39.0 33.0 39.0 96-97 36.5881390683051 39.0 37.0 39.0 33.0 39.0 98-99 36.81113457382612 38.0 37.5 39.0 33.5 39.0 100-101 36.49382557742142 38.5 37.5 39.0 32.5 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1871562115371006 1101 2 0.18927741082620742 1101 3 0.26484697696433557 1101 4 0.13048341593023594 1101 5 0.24540652493977433 1101 6 0.33875170844420666 1101 7 0.2649991318511553 1101 8 0.1794777158078631 1101 9 0.24137240613294608 1101 10-11 0.23489069427172637 1101 12-13 0.20390533747095674 1101 14-15 0.27621107352724295 1101 16-17 0.16246342176945205 1101 18-19 0.2568060958746514 1101 20-21 0.12238923056793993 1101 22-23 0.24885295703726484 1101 24-25 0.2755070118884788 1101 26-27 0.20455496355917546 1101 28-29 0.20244132480661392 1101 30-31 0.3105585911698725 1101 32-33 0.37186464060841473 1101 34-35 0.34328132919139165 1101 36-37 0.2731177679965313 1101 38-39 0.20818172938392365 1101 40-41 0.3252911518681145 1101 42-43 1.0309229141805503 1101 44-45 0.2894849247167386 1101 46-47 0.2997935689332536 1101 48-49 0.26662596833430285 1101 50-51 0.3651676059992752 1101 52-53 0.24227984804799263 1101 54-55 0.29350783514436785 1101 56-57 0.20657956301430858 1101 58-59 0.19372657374483992 1101 60-61 0.22892540379540804 1101 62-63 0.25812214903809405 1101 64-65 0.6214171131269879 1101 66-67 0.6962117294795291 1101 68-69 0.5321133387204569 1101 70-71 0.42236256508761727 1101 72-73 0.2645114477249848 1101 74-75 0.24936168640419254 1101 76-77 0.212263421894626 1101 78-79 0.27526341754519024 1101 80-81 0.32987554394581053 1101 82-83 0.28544292479555367 1101 84-85 0.2436624373370435 1101 86-87 0.36955711484056764 1101 88-89 0.4739440398246728 1101 90-91 0.4552424949148346 1101 92-93 0.3907621306378033 1101 94-95 0.29559435914174514 1101 96-97 0.30231262738935527 1101 98-99 0.34620090285449834 1101 100-101 0.3028544795079 1102 1 0.22919401851421384 1102 2 0.14418976370429704 1102 3 0.25709008039235215 1102 4 0.27627734640653756 1102 5 0.29661901054158335 1102 6 0.36428836677604437 1102 7 0.21352704845941162 1102 8 0.15118970136587961 1102 9 0.14335100399086542 1102 10-11 0.22372233978374823 1102 12-13 0.256785829971939 1102 14-15 0.1913796877324998 1102 16-17 0.16636490869657194 1102 18-19 0.20882802623054886 1102 20-21 0.10803326567327787 1102 22-23 0.15063699779801 1102 24-25 0.26618398396298204 1102 26-27 0.2251734430804646 1102 28-29 0.2656590450220335 1102 30-31 0.23982923041165094 1102 32-33 0.37875271227561313 1102 34-35 0.33032094125843514 1102 36-37 0.28373010394303577 1102 38-39 0.26507345943419836 1102 40-41 0.29832402580187534 1102 42-43 0.9270551188914524 1102 44-45 0.4314660594209627 1102 46-47 0.35056849103695953 1102 48-49 0.27014733174922156 1102 50-51 0.3276446306393552 1102 52-53 0.27081470774599836 1102 54-55 0.3819603494864907 1102 56-57 0.19694230293135462 1102 58-59 0.26440560594110707 1102 60-61 0.23653303833790318 1102 62-63 0.3058457843205744 1102 64-65 0.5484618322982584 1102 66-67 0.571143708315752 1102 68-69 0.4377817207693866 1102 70-71 0.3429513591740587 1102 72-73 0.20560742150804145 1102 74-75 0.16633675122334068 1102 76-77 0.2590958153134153 1102 78-79 0.25996239996334225 1102 80-81 0.18145846398216037 1102 82-83 0.2913402384585311 1102 84-85 0.3580585650494541 1102 86-87 0.40599112293703854 1102 88-89 0.33460155605894926 1102 90-91 0.4230249286611141 1102 92-93 0.406034602318023 1102 94-95 0.3804996940875043 1102 96-97 0.2820823750014938 1102 98-99 0.25099295065167126 1102 100-101 0.25642458378059985 1103 1 0.32077667202019455 1103 2 0.11379680555861427 1103 3 0.20492369509464226 1103 4 0.29645328553339567 1103 5 0.29236120176343405 1103 6 0.3331991275667434 1103 7 0.19576417085389153 1103 8 0.13460452881493978 1103 9 0.18040838021831007 1103 10-11 0.22952624342094907 1103 12-13 0.22785830580250632 1103 14-15 0.1374788883344351 1103 16-17 0.19305758846551413 1103 18-19 0.19549263787592253 1103 20-21 0.06884402316324412 1103 22-23 0.19684972006594847 1103 24-25 0.24511360589743703 1103 26-27 0.29243199091401095 1103 28-29 0.2623101292054244 1103 30-31 0.2854444148557391 1103 32-33 0.3381551800479272 1103 34-35 0.32859492215295205 1103 36-37 0.31012051828212606 1103 38-39 0.2740948372124805 1103 40-41 0.28168241711281183 1103 42-43 0.8563857536489152 1103 44-45 0.3091444383026669 1103 46-47 0.33828988119138614 1103 48-49 0.26897091920761085 1103 50-51 0.293923293628211 1103 52-53 0.26787763386585794 1103 54-55 0.2591977542474737 1103 56-57 0.0715624659928622 1103 58-59 0.16310570514544764 1103 60-61 0.2086919983242268 1103 62-63 0.2519326710003469 1103 64-65 0.5276505910595546 1103 66-67 0.5595389190423745 1103 68-69 0.4475782014794092 1103 70-71 0.289169507110941 1103 72-73 0.19532681475689628 1103 74-75 0.2230705975375642 1103 76-77 0.3874134881740332 1103 78-79 0.31152912467884164 1103 80-81 0.273651984561468 1103 82-83 0.31560716781093134 1103 84-85 0.31562825570112096 1103 86-87 0.39359737188586763 1103 88-89 0.14286780955050915 1103 90-91 0.5370792147974015 1103 92-93 0.4320948316938811 1103 94-95 0.44137924827256825 1103 96-97 0.44589158550836316 1103 98-99 0.6183452025420522 1103 100-101 0.44610844188548526 2112 1 0.18653312285813684 2112 2 0.13373533443560603 2112 3 0.2136101907833421 2112 4 0.2591373940714803 2112 5 0.313564277851178 2112 6 0.2949421930765368 2112 7 0.1188237124170044 2112 8 0.07256178728243157 2112 9 0.25450879443088326 2112 10-11 0.2331629886822384 2112 12-13 0.16555991944214554 2112 14-15 0.14150654323766076 2112 16-17 0.1794671698025283 2112 18-19 0.16290607110695277 2112 20-21 0.06783314848407684 2112 22-23 0.25817651268560127 2112 24-25 0.11747247664642657 2112 26-27 0.2347604978253628 2112 28-29 0.27863381311642854 2112 30-31 0.266811562647824 2112 32-33 0.28266094706048506 2112 34-35 0.28319034290461786 2112 36-37 0.2929372302590494 2112 38-39 0.35977555861265387 2112 40-41 0.36932319455445395 2112 42-43 0.9492094311052668 2112 44-45 0.3990081686332303 2112 46-47 0.31152196713131985 2112 48-49 0.23389582504020012 2112 50-51 0.008908619607602475 2112 52-53 0.0949682201510953 2112 54-55 0.017106470144881314 2112 56-57 -0.08872133294624973 2112 58-59 -0.14869222134048954 2112 60-61 0.03185630735942624 2112 62-63 0.1830145704245254 2112 64-65 0.6465207446952803 2112 66-67 0.5191327571191593 2112 68-69 0.46025138411724953 2112 70-71 0.3617224922391742 2112 72-73 0.3992871266547624 2112 74-75 0.24931375962690794 2112 76-77 0.2661332643945329 2112 78-79 0.19819748367785195 2112 80-81 0.3138136566232106 2112 82-83 0.2849216295298689 2112 84-85 0.3462164115639226 2112 86-87 0.3654114854971269 2112 88-89 0.05562434852130593 2112 90-91 0.2923797108002546 2112 92-93 0.351258778041327 2112 94-95 0.14137570423379486 2112 96-97 0.093125813909964 2112 98-99 -0.0062026400506240975 2112 100-101 0.10046684396510841 1113 1 0.18305621790255344 1113 2 0.09479404519037615 1113 3 0.17060966296415359 1113 4 0.28910473917792245 1113 5 0.2580166366693959 1113 6 0.2870054674304825 1113 7 0.05411940834347462 1113 8 0.08692816502102119 1113 9 0.22479519095599443 1113 10-11 0.20322367021027787 1113 12-13 0.23299560136426578 1113 14-15 0.08334148767124816 1113 16-17 0.18510197940072004 1113 18-19 0.12982683346790935 1113 20-21 0.17793682773277197 1113 22-23 0.14555726044311257 1113 24-25 0.2586602734469565 1113 26-27 0.2244592731965156 1113 28-29 0.2945023831645557 1113 30-31 0.29018382255707564 1113 32-33 0.20735962437418465 1113 34-35 0.32793400228606373 1113 36-37 0.331183695792852 1113 38-39 0.3441528205967188 1113 40-41 0.407971954648751 1113 42-43 0.9666023649694111 1113 44-45 0.31081056733362544 1113 46-47 0.33719968996422267 1113 48-49 0.33072634034615334 1113 50-51 0.23772093180595277 1113 52-53 0.2680869616982733 1113 54-55 0.3009446114489407 1113 56-57 0.2421470831088115 1113 58-59 0.169346422257469 1113 60-61 0.37525041277057625 1113 62-63 0.25812673784101037 1113 64-65 0.5075005902240264 1113 66-67 0.36413774687176215 1113 68-69 0.3136376523373272 1113 70-71 0.12615119768991434 1113 72-73 0.1547254236455231 1113 74-75 0.15045999204949823 1113 76-77 0.15477527443726302 1113 78-79 0.018640572518293652 1113 80-81 0.21454935468244685 1113 82-83 0.17024844694830676 1113 84-85 0.19729731750754098 1113 86-87 0.25147198422450856 1113 88-89 0.2622650019738728 1113 90-91 0.36237268555795055 1113 92-93 0.3083446908642813 1113 94-95 0.1806463321006504 1113 96-97 0.20598999783939576 1113 98-99 0.103440970561401 1113 100-101 0.2295801723228692 2113 1 0.04733847027106464 2113 2 0.04265104217440552 2113 3 0.14781896455055943 2113 4 0.20425343790348194 2113 5 0.13807860179081644 2113 6 0.16161320686002512 2113 7 0.1673804019678542 2113 8 0.0720794128342348 2113 9 0.12982385500720994 2113 10-11 0.16170630908094807 2113 12-13 0.11722415299966826 2113 14-15 0.05264026593326321 2113 16-17 0.0349947355911695 2113 18-19 0.04828400776155917 2113 20-21 0.08737163726656405 2113 22-23 0.06782206484454889 2113 24-25 0.14988553852429476 2113 26-27 0.08740418867095201 2113 28-29 0.10080070129691165 2113 30-31 0.10926376224136902 2113 32-33 0.004915622977968326 2113 34-35 0.13473919915079335 2113 36-37 0.13547618178140652 2113 38-39 0.1608727530459646 2113 40-41 0.21867334698222862 2113 42-43 0.8333916022248644 2113 44-45 0.2086485254104815 2113 46-47 0.2463976568372246 2113 48-49 0.2856700616240033 2113 50-51 0.32748671881914504 2113 52-53 0.25587646587308654 2113 54-55 0.16889611791474124 2113 56-57 0.2111040327241156 2113 58-59 0.09921734320916187 2113 60-61 0.21419298468764936 2113 62-63 0.17047582640188352 2113 64-65 0.5428080433161355 2113 66-67 0.541586097749871 2113 68-69 0.3383109287050843 2113 70-71 0.24395899819629818 2113 72-73 0.284703169020311 2113 74-75 0.11238024249549738 2113 76-77 0.22356546548044065 2113 78-79 0.11355795950557024 2113 80-81 0.1681218302231926 2113 82-83 0.26640343434694813 2113 84-85 0.09945509074606917 2113 86-87 0.2711943949776483 2113 88-89 0.46331037600140235 2113 90-91 0.2236289171631185 2113 92-93 0.08693015254750946 2113 94-95 0.19260349755511186 2113 96-97 0.16296448844062184 2113 98-99 0.20851706339465892 2113 100-101 0.27394629494930456 1112 1 0.0685987903224543 1112 2 0.04846170617226875 1112 3 0.14588899639716146 1112 4 0.16592114736113217 1112 5 0.14705902899633116 1112 6 0.17223861595088863 1112 7 0.16089049420981638 1112 8 -0.07237992634181722 1112 9 0.18438024166234612 1112 10-11 0.181201042853111 1112 12-13 0.14812824056232898 1112 14-15 0.22474302236140176 1112 16-17 0.08404010406108142 1112 18-19 0.1743241395289985 1112 20-21 0.16776127363156945 1112 22-23 0.20879940171200673 1112 24-25 0.2798471824211717 1112 26-27 0.11602696125204659 1112 28-29 0.18410651490880525 1112 30-31 0.198998935306129 1112 32-33 0.07971904217426129 1112 34-35 0.1546100029266384 1112 36-37 0.22389724494168917 1112 38-39 0.17852469708592622 1112 40-41 0.22956037279555375 1112 42-43 0.7723223772849366 1112 44-45 0.2337254117211529 1112 46-47 0.2761677003838301 1112 48-49 0.2796186007042678 1112 50-51 0.2599763125034613 1112 52-53 0.3031316587135038 1112 54-55 0.2846886220619922 1112 56-57 0.25292573597497636 1112 58-59 0.29793453940785497 1112 60-61 0.3619554953274289 1112 62-63 0.20636619725762273 1112 64-65 0.5891157934389355 1112 66-67 0.5117596342545951 1112 68-69 0.42193439150443623 1112 70-71 0.23524097043854653 1112 72-73 0.3277689055405517 1112 74-75 0.23918126971094722 1112 76-77 0.2040048382897126 1112 78-79 0.17274700898174444 1112 80-81 0.18902650469129867 1112 82-83 0.1651454587310397 1112 84-85 0.2565234936720273 1112 86-87 0.2969228253002356 1112 88-89 0.41220167517627715 1112 90-91 0.33511736042824936 1112 92-93 0.23223432843484204 1112 94-95 0.11087981631098387 1112 96-97 0.11238469048529964 1112 98-99 0.08291095502850254 1112 100-101 0.16762985740676584 2114 1 0.019358296118770824 2114 2 0.024694312302280252 2114 3 0.06974481613601569 2114 4 0.07251710305920511 2114 5 0.15051449826327 2114 6 0.056460909330617426 2114 7 -0.005503773506035259 2114 8 -0.13268914840261203 2114 9 0.16299211701343097 2114 10-11 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100-101 -0.3006532585302324 2109 1 -0.46432948923655104 2109 2 -0.24919668313032162 2109 3 -0.29429125856938754 2109 4 -0.5786892222011026 2109 5 -0.32102424463180057 2109 6 -0.475250979170589 2109 7 -0.32017524215049065 2109 8 -0.036023615437947853 2109 9 -0.30689072515733073 2109 10-11 -0.3127391890540139 2109 12-13 -0.22244291418287077 2109 14-15 -0.3663980189144169 2109 16-17 -0.24167134968271142 2109 18-19 -0.3442956159024604 2109 20-21 -0.3424460066950772 2109 22-23 -0.4655879436418431 2109 24-25 -0.5233680141324939 2109 26-27 -0.3994635929370034 2109 28-29 -0.2816675494405416 2109 30-31 -0.4675782805587332 2109 32-33 -0.25084633960405966 2109 34-35 -0.23037872438014517 2109 36-37 -0.20470274661555976 2109 38-39 -0.2937272407031628 2109 40-41 -0.5801380358632002 2109 42-43 -4.605147729680738 2109 44-45 -0.5421551876095805 2109 46-47 -0.6122143907321842 2109 48-49 -0.4218881959122669 2109 50-51 -0.34288857299523556 2109 52-53 -0.30710871683299246 2109 54-55 -0.37995613210914314 2109 56-57 -0.1385357738923787 2109 58-59 -0.08871063834244097 2109 60-61 -0.372231434277289 2109 62-63 -0.17296149493106583 2109 64-65 -4.344444719251342 2109 66-67 -3.063425014861515 2109 68-69 -0.8546366526830909 2109 70-71 -0.1078764244693744 2109 72-73 -0.5202231863868505 2109 74-75 -0.20506650761345924 2109 76-77 -0.5685553364653018 2109 78-79 -0.42122676866549114 2109 80-81 -0.16575970338716672 2109 82-83 -0.09969649267232938 2109 84-85 -0.13384054879058738 2109 86-87 -0.4564051342271753 2109 88-89 -0.23904798473722622 2109 90-91 -0.47775888313724835 2109 92-93 -0.44776375144772373 2109 94-95 -0.6890817452986369 2109 96-97 -0.8057255670550703 2109 98-99 -0.8352423271987064 2109 100-101 -0.7591544923656386 2108 1 -0.33677798017396654 2108 2 -0.21485443350099587 2108 3 -0.27291001589427566 2108 4 -0.28634085557802536 2108 5 -0.181814775610043 2108 6 -0.31332365965366904 2108 7 -0.36286888818615637 2108 8 0.021771912212990685 2108 9 -0.0354392086670714 2108 10-11 -0.1847901093119404 2108 12-13 -0.06161763132134013 2108 14-15 -0.34732586551476885 2108 16-17 -0.13004597949946373 2108 18-19 -0.20721937349374286 2108 20-21 -0.21724763577754658 2108 22-23 -0.08838214004868661 2108 24-25 -0.3441118427394798 2108 26-27 -0.24642032412111803 2108 28-29 -0.2514568342733128 2108 30-31 -0.37999533324043 2108 32-33 -0.09959326724099782 2108 34-35 -0.4041688931156884 2108 36-37 -0.6490724832494763 2108 38-39 -0.39320373725728075 2108 40-41 -0.4748684765765674 2108 42-43 -4.888722410547224 2108 44-45 -0.9287341867823713 2108 46-47 -0.39517103190818403 2108 48-49 -0.42260602942152303 2108 50-51 -0.4037844340075907 2108 52-53 -0.23651610317251226 2108 54-55 -0.43884447736479615 2108 56-57 -0.20298289797653268 2108 58-59 -0.4898857978550737 2108 60-61 -0.4142493253242705 2108 62-63 -0.3363191880379972 2108 64-65 -1.5133444361738029 2108 66-67 -0.7965527810462021 2108 68-69 -0.5563238467158556 2108 70-71 -0.6348452721594171 2108 72-73 -0.3710689627659036 2108 74-75 -0.330890637388201 2108 76-77 -0.4793848125454687 2108 78-79 -0.5078777564666765 2108 80-81 -0.5403948176581466 2108 82-83 -0.4893325515965401 2108 84-85 -0.39081006474095403 2108 86-87 -0.741303763945659 2108 88-89 -0.17894264187550846 2108 90-91 -0.7868656870710922 2108 92-93 -0.6789561142899885 2108 94-95 -0.4392391967340643 2108 96-97 -0.41506599801161315 2108 98-99 -0.5540881809339524 2108 100-101 -0.3956997121134975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 3.0 15 17.0 16 24.0 17 46.0 18 51.0 19 86.0 20 219.0 21 802.0 22 3160.0 23 10203.0 24 25613.0 25 50341.0 26 79303.0 27 116172.0 28 150735.0 29 189345.0 30 239852.0 31 302616.0 32 390989.0 33 522951.0 34 728365.0 35 1059781.0 36 1770028.0 37 5677111.0 38 4836895.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.33147523734411 50.86394313880863 1.6788788953115776 1.1257027285356858 2 1.0982370527379972 1.9160667022847644 5.819987224777617 91.16570902019963 3 1.238518131152966 52.053243422707276 45.16952301647773 1.5387154296620262 4 10.520461866567823 15.8215044294515 17.063705697205688 56.59432800677499 5 10.26885102046716 1.4165095762480155 25.9925201995282 62.32211920375662 6 1.1521717909001024 49.994425773933784 39.21782806486951 9.635574370296611 7 1.6265969260108617 35.80941012307928 53.92948272853445 8.634510222375408 8 64.91059665636813 1.5425099888831177 1.8255977250967503 31.721295629652012 9 57.324369012156154 1.6344398404205238 10.226640417973485 30.814550729449845 10-11 43.20111863358232 54.0196360082995 1.5265951246785072 1.2526502334396739 12-13 31.73071393610371 2.9388768315170517 55.97200481915211 9.358404413227127 14-15 57.599929531383076 24.594949373585127 11.0415204631665 6.763600631865296 16-17 38.1092287084837 20.63348216300275 37.104834262257526 4.152454866256025 18-19 41.465516339539136 5.928432384637817 42.447468413080045 10.158582862742993 20-21 5.227553154934577 33.97784200260122 26.39249026398433 34.40211457847987 22-23 20.39487062255346 20.51966395680665 22.068317665146427 37.01714775549346 24-25 46.29151784783001 25.914932915226142 18.732033489430233 9.061515747513619 26-27 18.378467851076735 75.19188367924177 3.094853023969667 3.3347954457118356 28-29 41.026576213783855 14.900571715652694 24.026452596577258 20.04639947398619 30-31 36.84944742737242 26.610055309569486 23.154508694647486 13.385988568410609 32-33 27.067318266147662 3.817923925463467 50.652970598232386 18.461787210156494 34-35 42.144364841236076 29.26624366926077 20.848753759662277 7.740637729840878 36-37 26.559125887071072 23.3182875655637 19.00427609064329 31.118310456721936 38-39 9.163031039432527 35.189046116522434 44.03162260613917 11.616300237905863 40-41 17.88824236945401 36.69322672417064 17.76152080486253 27.657010101512814 42-43 18.578403362140413 11.939397979870762 38.552040770279426 30.9301578877094 44-45 17.23961106325097 21.926854268931738 33.21174649447415 27.62178817334314 46-47 40.236726022115285 23.517272889285717 19.079746345167838 17.166254743431157 48-49 35.72436680846433 35.4043486639097 13.864780851205676 15.006503676420294 50-51 52.7451252758561 17.611654286066063 16.367552643628557 13.275667794449284 52-53 23.3309247477005 28.33047008150998 16.766804651201085 31.57180051958844 54-55 28.82491105225108 34.17819534848941 14.476156766426435 22.520736832833077 56-57 17.557456466718158 19.312780564478135 48.44108612541396 14.68867684338975 58-59 19.84742653055527 40.90613764692388 17.032860201938643 22.21357562058221 60-61 22.0252156816938 38.06899895256547 11.8363691973653 28.069416168375426 62-63 20.357677133026662 31.399534959125543 24.83229193419702 23.41049597365078 64-65 24.961904296759542 18.617320807202407 41.27539530424225 15.145379591795805 66-67 36.92094360845497 17.60933298148546 34.41114290576203 11.05858050429754 68-69 32.18833282605091 34.86601337108579 17.55930113009154 15.38635267277176 70-71 14.122569462563517 21.60407841453535 37.060014513415254 27.213337609485876 72-73 19.35095828714711 29.39807519900235 17.911210904510877 33.33975560933967 74-75 36.209841353378756 21.080438527243047 36.199027169105925 6.51069295027227 76-77 20.131637778185915 35.77647854876247 15.080163313371973 29.011720359679643 78-79 35.75446639119281 28.185484492478324 29.562596887384352 6.497452228944514 80-81 52.07397422014844 20.683430447431768 19.706724522243018 7.535870810176773 82-83 30.842718986767263 24.87625744295363 32.079503877166715 12.201519693112392 84-85 31.172285430830105 45.96539002961923 17.33977380836758 5.522550731183087 86-87 26.520239392736816 55.21432171882515 8.226718909019036 10.038719979419005 88-89 14.720676800801549 26.533545110592833 23.705589497155287 35.04018859145033 90-91 31.077529777849914 27.463332827598443 32.02172505861913 9.437412335932512 92-93 24.5675363140246 48.03299149492572 20.374217201931646 7.025254989118033 94-95 12.880652941504547 54.56643632516067 8.539485298063864 24.013425435270914 96-97 25.18157708690389 34.354976620129776 14.242977078695754 26.220469214270587 98-99 42.5778669241272 18.538031232874573 34.74583788541162 4.1382639575866085 100-101 23.703426041286164 55.41454197658404 12.19976788192223 8.682264100207563 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 4.0 2 6.0 3 8.0 4 9.0 5 6.5 6 4.5 7 3.0 8 3.0 9 3.5 10 3.0 11 5.5 12 5.0 13 5.0 14 16.0 15 24.0 16 47.5 17 139.5 18 625.0 19 1200.0 20 1461.5 21 1249.5 22 694.5 23 560.5 24 1406.0 25 1645.0 26 3692.0 27 5408.5 28 8741.0 29 16622.0 30 19729.0 31 13649.5 32 25453.0 33 96770.0 34 166030.5 35 149977.0 36 140609.0 37 536049.5 38 740695.5 39 394898.5 40 222004.5 41 219912.5 42 240351.5 43 635959.0 44 839392.0 45 645037.0 46 457635.5 47 315859.0 48 529387.5 49 1477914.5 50 2887545.5 51 2467501.5 52 1066024.0 53 775606.5 54 520163.5 55 196835.0 56 124231.0 57 67815.0 58 36743.5 59 25374.5 60 21700.5 61 22760.5 62 18330.0 63 5414.5 64 2189.5 65 1387.5 66 1203.0 67 835.5 68 510.0 69 466.5 70 389.0 71 180.5 72 86.5 73 45.5 74 26.0 75 21.0 76 15.0 77 10.0 78 8.0 79 6.0 80 5.0 81 4.0 82 3.5 83 2.5 84 2.0 85 3.0 86 4.5 87 4.5 88 3.5 89 2.0 90 3.0 91 3.5 92 2.0 93 3.5 94 3.5 95 10.0 96 13.5 97 8.5 98 8.5 99 45.0 100 137.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6154709E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 2.4462704617723876 #Duplication Level Percentage of deduplicated Percentage of total 1 72.4497273775451 1.7723162804715087 2 9.94499123701865 0.4865627661140792 3 4.059202310430626 0.2978972013109401 4 2.096918409024773 0.20518518258976207 5 1.2361823068773319 0.15120181313398331 6 0.9392953132220563 0.13786622277698157 7 0.7667723363558832 0.13130127621321191 8 0.6285229242627153 0.1230029651336547 9 0.35760064675089404 0.07873091093316727 >10 4.252830295784879 2.415639868516256 >50 2.1018245325980334 3.6021706172198944 >100 0.8674598041993683 4.120090322033765 >500 0.12400873432970676 2.1358544836264803 >1k 0.12393067102663625 6.013811783960811 >5k 0.022804272490580334 3.8633345180440495 >10k+ 0.027928828083044397 74.46503378792146 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG 2134537 13.213094708174564 No Hit ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG 998320 6.179746103752163 No Hit ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC 727820 4.505311732944246 No Hit ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC 646901 4.004411345323521 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA 635483 3.933732263453337 No Hit GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTGCAAAGCT 547238 3.387482869545963 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG 392728 2.4310434808822614 No Hit GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGTGTCACCGCT 356894 2.209225805305438 No Hit ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC 327690 2.028448794713665 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG 320016 1.9809456177762161 No Hit GCTATCCGGGACTGAGTTGTTTCGAGAGGAGTGGAGACTGTTGTTTTGGG 313188 1.9386793039726062 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG 271422 1.6801416850034254 No Hit GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT 241857 1.4971300318687264 No Hit GCTCCTACCGATTGGATGACTCGGTGAAATCACCGGATGCTTACGAGCAG 160144 0.9913146686826733 No Hit ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG 153566 0.9505958912661319 No Hit GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACTGATGCATTAGG 149157 0.9233035395437948 No Hit ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG 147814 0.914990174072464 No Hit GCTCCTACCGATACCGGGTGTTGTGCTGAACCTATTAGACCACTTGTGGA 141926 0.8785425970842311 No Hit GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTAATAATAGG 134987 0.8355891771247629 No Hit GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC 133991 0.8294237921586827 No Hit GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTTTTCTAAA 130925 0.8104448059076768 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG 120672 0.7469772436012311 No Hit ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG 104907 0.6493895990327031 No Hit GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGACAGGAC 101793 0.6301134857953802 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCATAA 98444 0.609382688354213 No Hit GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACTAGCAGACGTTTT 95329 0.5901003849713418 No Hit ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG 79246 0.49054427411846296 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG 78677 0.48702208130149544 No Hit ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG 74985 0.46416806393727056 No Hit GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGTCGCAAGACT 71918 0.4451828875407165 No Hit GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA 71153 0.44044742619628746 No Hit GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTGCGGAG 67078 0.4152225830870738 No Hit GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTG 61234 0.3790473725029649 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG 58214 0.3603531329471797 No Hit GCTATTACCGATTGAGTGTTAAGGTGAACCTTCTCGATAGAGCAATCTAA 57041 0.35309209221905513 No Hit ACTTCTACCGATTCGAGTGGTTCGGCGAATGCTTCGGATCGACCCGCAAG 56979 0.3527083031950622 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA 56542 0.35000320959046677 No Hit GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGCGATTGGG 54734 0.3388114264391887 No Hit GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC 52502 0.3249950215754428 No Hit GTTGCTACTGATGGTATGGTAACAGAGGAAGGAGGAGAAACGTTAGTTAC 51970 0.32170186414376145 No Hit GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGGCGTTTC 46970 0.2907511364023951 No Hit GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA 45766 0.2832982011622741 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTATGTG 43272 0.2678599781648806 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTGTCATGT 41566 0.2572995898595264 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT 36295 0.22467133267457806 No Hit GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAATGTCAAAGTT 36193 0.22403993782865417 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG 35275 0.21835738421533935 No Hit TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTAGTTC 34850 0.2157265723573232 No Hit GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGATGCAAGTCT 34393 0.21289767584176228 No Hit GTTGTTTCCGATGGTTGGCGAAATACAGGTTGTTGGACTGGCTTCGTGCC 33781 0.20910930676621906 No Hit ACACCATGGGAGTTGAATTCACCCGAAGCCGGAATACTAAACTAGTTACC 32610 0.20186064632919104 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACGGCATGTG 29834 0.1846768022871845 No Hit GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGCCGCAAGGCT 27005 0.16716488053111944 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTGTGTG 25921 0.1604547627567912 No Hit ACACCATGGGAGTGGAGGATACCAGAAGTAGCTAGACTAACCGCAAGGGG 25481 0.157731098715551 No Hit GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGTAAACTAA 25453 0.15755777464019935 No Hit GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGCAAACTAG 24487 0.15157809404056738 No Hit GTTGTTTCCGATGATGGTGCGATACAGGTGATCGGACCGGCTTCGGCCGG 23650 0.14639694221666263 No Hit ACACCATGGGAGCGGGTTCCGCCAGAAGTAGGTAGCCTAACCGCAAGGGG 23519 0.14558603314983884 No Hit GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTGTCAAAGCT 23186 0.14352471468226385 No Hit ACTACTACCGATTGAATGGCTTAGTGAGGCTTCCGGATTGGTTTAAAGAA 22987 0.14229287571815746 No Hit GCTTTTACCGATTGAGTGTCCAGGTGAGTCTTCTTGATAGTTGCAAAATT 21736 0.13454900363726763 No Hit ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGTAAGGAG 21012 0.1300673382603178 No Hit GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACCGCGCGGGAC 20899 0.1293678518133629 No Hit GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTCCGTGCC 20835 0.1289716824982734 No Hit GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTTAATGGATT 19974 0.12364196718121012 No Hit GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGGCGCAAGCTT 19566 0.12111638779751464 No Hit GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG 19434 0.12029928858514258 No Hit GCTGCTACCGATTGAATAGTAAATGTGAAATGGGAGGACTTTGCTGTCTG 19277 0.11932743573406367 No Hit GCTGCTACCGATTGAATAGGAAATGTGAAATGGGAGGACGGGAGACTCTG 19176 0.11870223103368809 No Hit ACTTCTACCGATTCGAGTGGTTCGACGAATGACTAGGATTGATGGCTCAC 18797 0.11635616587089251 No Hit ACTCCTACCGATTCGAGTGGTTCGACGAATTCTCCGGATATCGTTCCCAG 17807 0.11022792177810198 No Hit GCACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG 17611 0.10901465325064041 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACTTTCGGGAG 17328 0.10726284206047908 No Hit ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAACTAGCTACC 17305 0.1071204687128688 No Hit GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGGCGTTTT 17049 0.10553579145251085 No Hit ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCTTAGGGAG 16725 0.1035301842948703 No Hit GCTCCTACCAATTTCGAGCGGCTCGGTGAGCCTTCCTTCTCTCGACTGCA 16356 0.10124602058755748 No Hit GCTACCGCGAAGAGGTCTCATGTAAAGCTCCACGGAGTTGGCGCTTGGTT 16255 0.10062081588718187 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.280777140584829E-4 0.0 0.0 2 3.3426785960675613E-4 0.0 0.0 3 3.3426785960675613E-4 0.0 0.0 4 3.3426785960675613E-4 0.0 0.0 5 3.404580051550294E-4 0.0 0.0 6 3.404580051550294E-4 0.0 0.0 7 3.528382962515759E-4 0.0 0.0 8 3.528382962515759E-4 0.0 0.0 9 3.6521858734812245E-4 0.0 0.0 10-11 3.6521858734812245E-4 0.0 0.0 12-13 3.683136601222591E-4 0.0 0.0 14-15 3.899791695412155E-4 0.0 0.0 16-17 4.02359460637762E-4 0.0 0.0 18-19 4.209298972825818E-4 0.0 0.0 20-21 4.3331018837912833E-4 0.0 0.0 22-23 4.425954067015382E-4 0.0 0.0 24-25 4.6735598889463125E-4 0.0 0.0 26-27 4.921165710877243E-4 0.0 0.0 28-29 5.014017894101342E-4 0.0 0.0 30-31 5.323525171515005E-4 0.0 0.0 32-33 5.571130993445936E-4 0.0 0.0 34-35 5.911588998600965E-4 0.0 0.0 36-37 6.12824409279053E-4 0.0 0.0 38-39 6.561554281169658E-4 0.0 0.0 40-41 7.21151956373835E-4 0.0 0.0 42-43 8.04718921275524E-4 0.0 0.0 44-45 8.913809589513497E-4 0.0 0.0 46-47 0.0010306592337874982 0.0 0.0 48-49 0.00114827199920469 0.0 0.0 50-51 0.001265884764621882 0.0 0.0 52-53 0.0014051630394580304 0.0 0.0 54-55 0.0016218181336475946 0.0 0.0 56-57 0.0018322830822888854 0.0 0.0 58-59 0.0020582233948008596 0.0 0.0 60-61 0.0023367799444731566 0.0 0.0 62-63 0.002606051275823043 0.0 0.0 64-65 0.0028536570977539736 0.0 0.0 66-67 0.003218875685102096 0.0 0.0 68-69 0.0036026647090950385 0.0 0.0 70-71 0.004039069970248303 0.0 0.0 72-73 0.004469285085853295 0.0 0.0 74-75 0.005048063694616845 0.0 0.0 76-77 0.005605176793961438 0.0 0.0 78-79 0.006202525839369808 0.0 0.0 80-81 0.006799874884778179 0.0 0.0 82-83 0.007483885967862374 0.0 0.0 84-85 0.008356696490168904 0.0 0.0 86-87 0.009235697158023706 0.0 0.0 88-89 0.010145648553619876 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAGTC 40 9.094947E-12 95.00001 8 CCAGTAC 90 0.0 95.0 2 CCCGAGT 2685 0.0 95.0 7 CCATGGG 763565 0.0 94.98444 4 GCTATCC 88330 0.0 94.94623 1 CTCCTAC 80140 0.0 94.940735 2 GCTATTA 100405 0.0 94.92431 1 CACCATG 763580 0.0 94.9241 2 ACCATGG 762295 0.0 94.91961 3 TATCCGG 88435 0.0 94.90868 3 CTATCCG 88480 0.0 94.898 2 ACACCAT 757925 0.0 94.89409 1 CTGTCCG 26405 0.0 94.89207 2 GCTGTCC 26340 0.0 94.8918 1 CGATTGA 296745 0.0 94.88795 9 CCGGGAC 88885 0.0 94.88778 6 AGTAACG 126765 0.0 94.88759 7 ATCCGGG 88440 0.0 94.887215 4 TTGTAGT 126400 0.0 94.8835 3 TACTACC 23810 0.0 94.86035 3 >>END_MODULE