FastQCFastQC Report
Thu 16 Jul 2015
000000000-AGRGJ l01n01.3310000000e389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGRGJ l01n01.3310000000e389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16154709
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG213453713.213094708174564No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG9983206.179746103752163No Hit
ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC7278204.505311732944246No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC6469014.004411345323521No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA6354833.933732263453337No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTGCAAAGCT5472383.387482869545963No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG3927282.4310434808822614No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGTGTCACCGCT3568942.209225805305438No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC3276902.028448794713665No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG3200161.9809456177762161No Hit
GCTATCCGGGACTGAGTTGTTTCGAGAGGAGTGGAGACTGTTGTTTTGGG3131881.9386793039726062No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG2714221.6801416850034254No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT2418571.4971300318687264No Hit
GCTCCTACCGATTGGATGACTCGGTGAAATCACCGGATGCTTACGAGCAG1601440.9913146686826733No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG1535660.9505958912661319No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACTGATGCATTAGG1491570.9233035395437948No Hit
ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG1478140.914990174072464No Hit
GCTCCTACCGATACCGGGTGTTGTGCTGAACCTATTAGACCACTTGTGGA1419260.8785425970842311No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTAATAATAGG1349870.8355891771247629No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC1339910.8294237921586827No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTTTTCTAAA1309250.8104448059076768No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG1206720.7469772436012311No Hit
ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG1049070.6493895990327031No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGACAGGAC1017930.6301134857953802No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCATAA984440.609382688354213No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACTAGCAGACGTTTT953290.5901003849713418No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG792460.49054427411846296No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG786770.48702208130149544No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG749850.46416806393727056No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGTCGCAAGACT719180.4451828875407165No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA711530.44044742619628746No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTGCGGAG670780.4152225830870738No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTG612340.3790473725029649No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG582140.3603531329471797No Hit
GCTATTACCGATTGAGTGTTAAGGTGAACCTTCTCGATAGAGCAATCTAA570410.35309209221905513No Hit
ACTTCTACCGATTCGAGTGGTTCGGCGAATGCTTCGGATCGACCCGCAAG569790.3527083031950622No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA565420.35000320959046677No Hit
GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGCGATTGGG547340.3388114264391887No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC525020.3249950215754428No Hit
GTTGCTACTGATGGTATGGTAACAGAGGAAGGAGGAGAAACGTTAGTTAC519700.32170186414376145No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGGCGTTTC469700.2907511364023951No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA457660.2832982011622741No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTATGTG432720.2678599781648806No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTGTCATGT415660.2572995898595264No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT362950.22467133267457806No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAATGTCAAAGTT361930.22403993782865417No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG352750.21835738421533935No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTAGTTC348500.2157265723573232No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGATGCAAGTCT343930.21289767584176228No Hit
GTTGTTTCCGATGGTTGGCGAAATACAGGTTGTTGGACTGGCTTCGTGCC337810.20910930676621906No Hit
ACACCATGGGAGTTGAATTCACCCGAAGCCGGAATACTAAACTAGTTACC326100.20186064632919104No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACGGCATGTG298340.1846768022871845No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGCCGCAAGGCT270050.16716488053111944No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTGTGTG259210.1604547627567912No Hit
ACACCATGGGAGTGGAGGATACCAGAAGTAGCTAGACTAACCGCAAGGGG254810.157731098715551No Hit
GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGTAAACTAA254530.15755777464019935No Hit
GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGCAAACTAG244870.15157809404056738No Hit
GTTGTTTCCGATGATGGTGCGATACAGGTGATCGGACCGGCTTCGGCCGG236500.14639694221666263No Hit
ACACCATGGGAGCGGGTTCCGCCAGAAGTAGGTAGCCTAACCGCAAGGGG235190.14558603314983884No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTGTCAAAGCT231860.14352471468226385No Hit
ACTACTACCGATTGAATGGCTTAGTGAGGCTTCCGGATTGGTTTAAAGAA229870.14229287571815746No Hit
GCTTTTACCGATTGAGTGTCCAGGTGAGTCTTCTTGATAGTTGCAAAATT217360.13454900363726763No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGTAAGGAG210120.1300673382603178No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACCGCGCGGGAC208990.1293678518133629No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTCCGTGCC208350.1289716824982734No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTTAATGGATT199740.12364196718121012No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGGCGCAAGCTT195660.12111638779751464No Hit
GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG194340.12029928858514258No Hit
GCTGCTACCGATTGAATAGTAAATGTGAAATGGGAGGACTTTGCTGTCTG192770.11932743573406367No Hit
GCTGCTACCGATTGAATAGGAAATGTGAAATGGGAGGACGGGAGACTCTG191760.11870223103368809No Hit
ACTTCTACCGATTCGAGTGGTTCGACGAATGACTAGGATTGATGGCTCAC187970.11635616587089251No Hit
ACTCCTACCGATTCGAGTGGTTCGACGAATTCTCCGGATATCGTTCCCAG178070.11022792177810198No Hit
GCACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG176110.10901465325064041No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACTTTCGGGAG173280.10726284206047908No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAACTAGCTACC173050.1071204687128688No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGGCGTTTT170490.10553579145251085No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCTTAGGGAG167250.1035301842948703No Hit
GCTCCTACCAATTTCGAGCGGCTCGGTGAGCCTTCCTTCTCTCGACTGCA163560.10124602058755748No Hit
GCTACCGCGAAGAGGTCTCATGTAAAGCTCCACGGAGTTGGCGCTTGGTT162550.10062081588718187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTC409.094947E-1295.000018
CCAGTAC900.095.02
CCCGAGT26850.095.07
CCATGGG7635650.094.984444
GCTATCC883300.094.946231
CTCCTAC801400.094.9407352
GCTATTA1004050.094.924311
CACCATG7635800.094.92412
ACCATGG7622950.094.919613
TATCCGG884350.094.908683
CTATCCG884800.094.8982
ACACCAT7579250.094.894091
CTGTCCG264050.094.892072
GCTGTCC263400.094.89181
CGATTGA2967450.094.887959
CCGGGAC888850.094.887786
AGTAACG1267650.094.887597
ATCCGGG884400.094.8872154
TTGTAGT1264000.094.88353
TACTACC238100.094.860353
Produced by FastQC (version 0.11.2)