##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AGRBU l01n03.3330000000e59e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16710914 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.116955422067278 32.0 31.0 33.0 18.0 33.0 2 31.285864256138233 32.0 32.0 33.0 29.0 33.0 3 31.078305950231087 33.0 32.0 33.0 29.0 34.0 4 31.503267684819633 32.0 32.0 33.0 29.0 34.0 5 32.053329339137285 33.0 32.0 33.0 32.0 34.0 6 36.25127578300026 37.0 37.0 37.0 34.0 37.0 7 36.43562458642298 37.0 37.0 37.0 37.0 37.0 8 36.38302842082725 37.0 37.0 37.0 37.0 37.0 9 36.4304658620109 37.0 37.0 37.0 37.0 37.0 10-11 36.375725917804374 37.0 37.0 37.0 37.0 37.0 12-13 36.84526714098343 37.5 37.5 37.5 37.0 37.5 14-15 37.313995841280736 38.0 38.0 38.0 37.0 38.0 16-17 37.149398770169 38.0 38.0 38.0 36.5 38.0 18-19 37.516706177771006 38.0 38.0 38.0 38.0 38.0 20-21 36.85150207822265 38.0 38.0 38.0 36.0 38.0 22-23 36.91726957603875 38.5 38.0 38.5 34.5 38.5 24-25 36.8662363111916 38.5 38.0 39.0 34.0 39.0 26-27 36.04914207565187 38.0 37.0 38.0 32.0 39.0 28-29 37.900843035874644 39.0 38.0 39.0 37.0 39.0 30-31 36.47276034692058 39.0 37.0 39.0 32.5 39.0 32-33 35.73455162296928 38.0 36.0 39.0 31.0 39.0 34-35 35.87572187852801 38.0 37.0 39.0 31.0 39.0 36-37 36.23875627030335 39.0 37.0 39.0 32.5 39.0 38-39 36.7011188316809 39.0 37.0 39.0 33.0 39.0 40-41 35.96905268018254 38.0 36.5 39.0 32.5 39.0 42-43 36.822225193666846 38.5 37.0 39.0 34.0 39.0 44-45 36.06826110768088 38.0 36.5 39.0 32.5 39.0 46-47 36.15558753997537 39.0 37.0 39.0 32.0 39.0 48-49 36.20970414903697 38.5 37.0 39.0 32.5 39.0 50-51 35.984471046885886 38.5 37.0 39.0 32.0 39.0 52-53 36.270391224561386 38.0 37.0 39.0 32.5 39.0 54-55 35.951286686054395 38.0 36.5 39.0 31.0 39.0 56-57 36.15273102356939 38.5 37.0 39.0 32.0 39.0 58-59 35.6643907089702 38.0 36.5 39.0 31.0 39.0 60-61 35.28795815716603 38.0 36.0 38.5 29.5 39.0 62-63 35.57538064644459 38.0 36.5 39.0 31.0 39.0 64-65 36.040426155026594 38.5 37.0 39.0 31.5 39.0 66-67 35.605177999240496 38.5 37.0 39.0 23.5 39.0 68-69 35.26410156260752 38.0 36.0 39.0 20.0 39.0 70-71 35.30973467399808 38.0 36.0 39.0 20.0 39.0 72-73 35.41323580505531 38.0 36.5 39.0 25.0 39.0 74-75 35.84909281443254 38.0 37.0 39.0 30.5 39.0 76-77 35.97294708117103 39.0 37.0 39.0 30.5 39.0 78-79 35.83667416396255 39.0 37.0 39.0 24.5 39.0 80-81 35.7228350585731 38.5 37.0 39.0 28.0 39.0 82-83 35.37622149213382 38.0 36.5 39.0 19.5 39.0 84-85 35.41293058536475 38.0 37.0 39.0 19.5 39.0 86-87 35.827835239891726 38.0 37.0 39.0 30.0 39.0 88-89 36.328814958894526 39.0 37.0 39.0 32.0 39.0 90-91 35.78988875174632 38.0 37.0 39.0 30.0 39.0 92-93 35.64103366817638 38.0 36.5 39.0 29.5 39.0 94-95 35.92680606817796 39.0 37.0 39.0 30.0 39.0 96-97 35.504144207791384 38.5 36.5 39.0 24.0 39.0 98-99 35.342692476306205 38.0 36.0 39.0 24.0 39.0 100-101 34.96011854887171 38.0 35.0 38.5 18.5 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0828745334399592 1101 2 0.7633043076493671 1101 3 0.8016276065992045 1101 4 0.6522546836598302 1101 5 0.45954048469265985 1101 6 0.1836320034783938 1101 7 0.13806375953326722 1101 8 0.2990334287841563 1101 9 0.25474592717683464 1101 10-11 0.22697362691121015 1101 12-13 0.250508000791541 1101 14-15 0.22952665355482083 1101 16-17 0.2505458138724421 1101 18-19 0.20380782217253568 1101 20-21 0.4234116834705972 1101 22-23 0.2741337547628788 1101 24-25 0.5819733219748215 1101 26-27 0.857694412771572 1101 28-29 0.3797058900381103 1101 30-31 0.6490044524918162 1101 32-33 0.9648214937046831 1101 34-35 0.9459873851176113 1101 36-37 0.819141444873047 1101 38-39 0.5356222258162973 1101 40-41 0.6614846929645495 1101 42-43 0.4968566760195756 1101 44-45 0.8135799098506666 1101 46-47 0.8116730765103313 1101 48-49 0.7381239643216659 1101 50-51 0.848179939559202 1101 52-53 0.8205114989655655 1101 54-55 0.9009420421554282 1101 56-57 0.7408361473361467 1101 58-59 0.6798379898697604 1101 60-61 0.871799531141285 1101 62-63 0.9490746052877697 1101 64-65 0.9200156193064473 1101 66-67 0.780462108079 1101 68-69 0.814587703507982 1101 70-71 0.8910870418697101 1101 72-73 0.7607729983663134 1101 74-75 0.7071109143939651 1101 76-77 0.8031447820707811 1101 78-79 0.7635310666912147 1101 80-81 0.7885597857230664 1101 82-83 0.9984142573305306 1101 84-85 0.9414764849129185 1101 86-87 0.7646961657979503 1101 88-89 0.6513978499585136 1101 90-91 0.6373064478763908 1101 92-93 0.8222289306211366 1101 94-95 0.6855790001226651 1101 96-97 0.9136945781344536 1101 98-99 1.0524804994522867 1101 100-101 1.0556779768850646 1102 1 0.9030111666890868 1102 2 0.8345049479012694 1102 3 0.6936071822216867 1102 4 0.5455105804150371 1102 5 0.3882741990634031 1102 6 0.13924395835424264 1102 7 0.13242277006611403 1102 8 0.30238603682792586 1102 9 0.2983813399034929 1102 10-11 0.2670096266075248 1102 12-13 0.29020980471312896 1102 14-15 0.22779785643182038 1102 16-17 0.20731772223117417 1102 18-19 0.20411905435994981 1102 20-21 0.36322151787840795 1102 22-23 0.2478509581784749 1102 24-25 0.8063299671729425 1102 26-27 0.8976406067029998 1102 28-29 0.322361505566775 1102 30-31 -0.29045610781998477 1102 32-33 -0.9118338571228222 1102 34-35 0.6566360277205519 1102 36-37 0.6556739721644718 1102 38-39 0.5325563866281016 1102 40-41 0.6829904290455886 1102 42-43 0.585703061329852 1102 44-45 0.7630065227266556 1102 46-47 0.676189238812654 1102 48-49 0.8158992320564309 1102 50-51 0.725780682369404 1102 52-53 0.6402236011692892 1102 54-55 0.7464244968204099 1102 56-57 0.6041775204620521 1102 58-59 0.563467142173927 1102 60-61 0.879399820229402 1102 62-63 0.866170751771044 1102 64-65 1.0015318997829326 1102 66-67 0.9777068501223809 1102 68-69 0.8977228146369001 1102 70-71 0.7977598848515441 1102 72-73 0.952313967401814 1102 74-75 0.8235494288205274 1102 76-77 0.706109999905614 1102 78-79 0.8500621959456964 1102 80-81 0.9807718858776582 1102 82-83 0.8308924705491378 1102 84-85 0.9126321245232774 1102 86-87 0.8192659143606207 1102 88-89 0.4039820123201707 1102 90-91 0.5443887450800347 1102 92-93 0.7191697679171227 1102 94-95 0.8705191877099736 1102 96-97 0.9519424241820147 1102 98-99 1.1362012591209378 1102 100-101 0.9943951412650875 1103 1 0.5783443513974369 1103 2 0.6466065279397846 1103 3 0.40440286467734055 1103 4 0.45658097448195534 1103 5 0.3220468733993229 1103 6 0.18169753507431352 1103 7 0.1497359198435646 1103 8 0.3182928453168259 1103 9 0.2981717373820345 1103 10-11 0.3049605652919851 1103 12-13 0.27161197125762016 1103 14-15 0.23504462278835803 1103 16-17 0.04335031253294375 1103 18-19 0.20192687898450856 1103 20-21 0.20552442411074168 1103 22-23 0.1967562172047863 1103 24-25 0.4955359788360596 1103 26-27 0.8896875266751962 1103 28-29 0.41265850541324767 1103 30-31 0.7770871164344229 1103 32-33 0.1460862840904582 1103 34-35 0.8910859530030493 1103 36-37 0.762065702161209 1103 38-39 0.6916327330016401 1103 40-41 0.8561114506652103 1103 42-43 0.5857685571191809 1103 44-45 0.7547528401320136 1103 46-47 1.1809192633817673 1103 48-49 0.703331629396942 1103 50-51 0.7769888512659406 1103 52-53 0.9224889482291232 1103 54-55 0.9219222733377848 1103 56-57 0.7341627561913597 1103 58-59 1.087815460639149 1103 60-61 1.0519632171255466 1103 62-63 1.0510542124028248 1103 64-65 1.040232337240603 1103 66-67 0.9497999491810489 1103 68-69 0.8532407527920896 1103 70-71 0.6164492919451448 1103 72-73 0.8845946189453073 1103 74-75 0.8800005017799748 1103 76-77 0.5728759710533353 1103 78-79 0.9851709618067304 1103 80-81 0.6850483060870545 1103 82-83 1.209987542537604 1103 84-85 1.071754010353139 1103 86-87 0.9044765672121073 1103 88-89 0.850352619192769 1103 90-91 0.968732530205088 1103 92-93 1.0505887210388352 1103 94-95 0.7240500983994167 1103 96-97 0.8992457923501789 1103 98-99 1.1279773987649122 1103 100-101 1.0214626790486108 2112 1 0.6013499485186387 2112 2 0.5380916022914022 2112 3 0.7416664444446823 2112 4 0.5916355685506751 2112 5 0.38615749330847393 2112 6 0.17445545304992294 2112 7 0.13936412408955334 2112 8 0.28865173051799076 2112 9 0.2640892392799472 2112 10-11 0.2735468406807229 2112 12-13 0.28159986309050566 2112 14-15 0.1850795123749478 2112 16-17 0.12397608191110976 2112 18-19 0.20267103162521494 2112 20-21 0.46253043139873284 2112 22-23 0.36805871177977423 2112 24-25 0.5938403847197051 2112 26-27 0.8180984543257921 2112 28-29 0.3951040140544535 2112 30-31 0.6217570176214267 2112 32-33 0.09385412210101407 2112 34-35 0.8932134898672786 2112 36-37 0.878784566822894 2112 38-39 0.6955535616272428 2112 40-41 0.8345729824362493 2112 42-43 0.7106447081442795 2112 44-45 0.7164072715925585 2112 46-47 1.1382643114629047 2112 48-49 0.8231730846138348 2112 50-51 0.8875717591226646 2112 52-53 0.5126674003484979 2112 54-55 0.5667536680114935 2112 56-57 0.6273242151153653 2112 58-59 0.689869385609704 2112 60-61 0.7654339577703624 2112 62-63 0.1376166659562159 2112 64-65 0.6063981557926823 2112 66-67 0.841240144115595 2112 68-69 0.7974477021352371 2112 70-71 0.26933904611525605 2112 72-73 0.7422355872593016 2112 74-75 0.6753275587179886 2112 76-77 0.6676412591123366 2112 78-79 0.9586891869200826 2112 80-81 0.9369054975295938 2112 82-83 1.1999074461146577 2112 84-85 0.8324704247485997 2112 86-87 0.688401873696499 2112 88-89 0.5797657168442711 2112 90-91 0.202985507889629 2112 92-93 0.7541698548598177 2112 94-95 0.6916046496536978 2112 96-97 0.9796608348248128 2112 98-99 1.1031449070781747 2112 100-101 0.8538957886202567 1113 1 -6.002020622704922 1113 2 -4.818766214127429 1113 3 -5.303096791968809 1113 4 -4.534222722917786 1113 5 -3.149862242759472 1113 6 -1.6389240131110938 1113 7 -1.1450603491443871 1113 8 -1.336112518260677 1113 9 -1.7197395064593408 1113 10-11 -0.758355121517134 1113 12-13 -1.410343442042631 1113 14-15 -0.9034552898335946 1113 16-17 -2.9297569410304547 1113 18-19 -2.0207968673063945 1113 20-21 -3.8813580516507997 1113 22-23 -4.398293279898013 1113 24-25 -5.469754861998403 1113 26-27 -9.987568074277974 1113 28-29 -3.558404345066677 1113 30-31 -7.069147047566034 1113 32-33 -10.170811357750722 1113 34-35 -11.473999105194217 1113 36-37 -8.556457128246528 1113 38-39 -6.140173827091676 1113 40-41 -7.62192680236501 1113 42-43 -5.345705347033984 1113 44-45 -10.920110825594854 1113 46-47 -13.075029116428247 1113 48-49 -7.466991360028551 1113 50-51 -10.617254146467559 1113 52-53 -7.675701896384922 1113 54-55 -7.523551801315108 1113 56-57 -7.501837204199017 1113 58-59 -8.96475135468944 1113 60-61 -10.317176675225092 1113 62-63 -11.495250232187026 1113 64-65 -10.585453023304723 1113 66-67 -8.128284175565742 1113 68-69 -8.71688643271479 1113 70-71 -8.498609974768197 1113 72-73 -9.368316605832852 1113 74-75 -9.402277250267261 1113 76-77 -9.416493422983045 1113 78-79 -11.64587060668537 1113 80-81 -9.689060453785892 1113 82-83 -9.05929496312526 1113 84-85 -11.29001414623853 1113 86-87 -8.71232692993135 1113 88-89 -6.129043564559161 1113 90-91 -5.34829290112895 1113 92-93 -9.805672892445081 1113 94-95 -6.493575776684878 1113 96-97 -10.483044020747133 1113 98-99 -9.19554009494166 1113 100-101 -11.405768221121576 2113 1 0.6443691694751585 2113 2 0.42814845946788793 2113 3 0.723297687933627 2113 4 0.48859197782386943 2113 5 0.33120241347943136 2113 6 0.12034603055547421 2113 7 0.11854560872995279 2113 8 0.2782769061836987 2113 9 0.2603069672929408 2113 10-11 0.2715278359022122 2113 12-13 0.26997719252656793 2113 14-15 0.18536163984982323 2113 16-17 0.21586105969806368 2113 18-19 0.18572763350255883 2113 20-21 0.5202473960436791 2113 22-23 0.45592331824642685 2113 24-25 0.441271238953334 2113 26-27 0.9414398614523805 2113 28-29 0.3704349670689595 2113 30-31 0.50618398908221 2113 32-33 1.0142619161106907 2113 34-35 0.6913620989253744 2113 36-37 0.9945985781929849 2113 38-39 0.635127610014024 2113 40-41 0.5520126962583873 2113 42-43 0.6433342289744246 2113 44-45 0.6228075233168369 2113 46-47 0.943210892562206 2113 48-49 0.8527099814105839 2113 50-51 0.744798175063309 2113 52-53 0.8278249860212483 2113 54-55 0.7514745707384733 2113 56-57 0.4147495181945686 2113 58-59 0.9939851315637895 2113 60-61 0.9137744796125986 2113 62-63 0.8807580898779079 2113 64-65 0.7640010958135761 2113 66-67 1.028431462325905 2113 68-69 0.46480629018595465 2113 70-71 -0.318117334660208 2113 72-73 0.5238380463600052 2113 74-75 0.5189659391164128 2113 76-77 0.7075175688694415 2113 78-79 0.567650338619373 2113 80-81 0.7244842667583953 2113 82-83 1.1328351505908927 2113 84-85 0.95514660392206 2113 86-87 0.9178492977551187 2113 88-89 0.26861922808434713 2113 90-91 -0.06349747492168234 2113 92-93 0.8704070995986157 2113 94-95 0.4843630911350516 2113 96-97 1.0603808593497135 2113 98-99 1.1005154270756492 2113 100-101 0.6958833244911347 1112 1 0.6168432533431805 1112 2 0.3983849542529647 1112 3 0.5233758409037783 1112 4 0.3874361421835424 1112 5 0.2160921164251306 1112 6 0.14026017852631867 1112 7 0.1321137960853136 1112 8 0.2720625777924113 1112 9 0.2738626883655684 1112 10-11 0.25293751680468546 1112 12-13 0.2738693943209398 1112 14-15 0.20853953512078505 1112 16-17 0.21301947616416328 1112 18-19 0.19586431353341993 1112 20-21 0.2817415980988969 1112 22-23 0.09380463234843006 1112 24-25 0.3856459650095232 1112 26-27 0.6206771833099936 1112 28-29 0.3464774492789857 1112 30-31 0.6377925444344257 1112 32-33 0.6087052103848762 1112 34-35 0.7984833206923909 1112 36-37 0.6612950845740286 1112 38-39 0.33764032666024946 1112 40-41 0.5741074266251474 1112 42-43 0.4756583452926435 1112 44-45 0.63102053138104 1112 46-47 0.7593458399140758 1112 48-49 0.5392016077926058 1112 50-51 0.6986267988157309 1112 52-53 0.6725816723870892 1112 54-55 0.596383488609213 1112 56-57 0.40420610523795375 1112 58-59 0.601955313289956 1112 60-61 0.8260480697572135 1112 62-63 0.927541703463298 1112 64-65 0.8497541773602322 1112 66-67 0.6675340142655628 1112 68-69 0.6070958882097486 1112 70-71 0.6049755652264608 1112 72-73 0.6646651858067187 1112 74-75 0.6235127220963079 1112 76-77 0.7425958460140336 1112 78-79 0.49007396639848366 1112 80-81 0.6650795687548907 1112 82-83 0.8197378932439037 1112 84-85 0.8769255600504877 1112 86-87 0.6531918539887371 1112 88-89 0.36602915455183904 1112 90-91 0.35096725671905205 1112 92-93 0.5931922581239419 1112 94-95 0.43852528624011455 1112 96-97 0.7138282627109334 1112 98-99 0.43565032263322934 1112 100-101 0.4710315468834665 2114 1 1.010613426227195 2114 2 0.5333002311114932 2114 3 0.47804943610712414 2114 4 0.4100377861397426 2114 5 0.30873020826654596 2114 6 0.14114870966806592 2114 7 0.11202858064945076 2114 8 0.3049855729303985 2114 9 0.3028673868361267 2114 10-11 0.2808683503787961 2114 12-13 0.27291276421355093 2114 14-15 0.22160207827335654 2114 16-17 0.20978374030398328 2114 18-19 0.22083423354020937 2114 20-21 0.3580030281514581 2114 22-23 0.3844809400022555 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-0.12222042647979947 2109 38-39 0.15478690934456552 2109 40-41 -0.03462252672247246 2109 42-43 0.07845615130549533 2109 44-45 0.06233346349750235 2109 46-47 -0.17088941070575459 2109 48-49 -0.06888248466903946 2109 50-51 0.047545786284231895 2109 52-53 -0.25024677989734556 2109 54-55 0.04976729557608195 2109 56-57 0.369349167097198 2109 58-59 0.24037868377325822 2109 60-61 0.1666313428602777 2109 62-63 0.4998887234453733 2109 64-65 -0.03870019598850405 2109 66-67 -0.26089487747601936 2109 68-69 0.007902059056767996 2109 70-71 0.3593520653746154 2109 72-73 -0.25478676513684206 2109 74-75 0.17035213533068827 2109 76-77 0.37103833465494773 2109 78-79 0.11018830891518405 2109 80-81 0.06651906608540514 2109 82-83 -1.7989687658657019 2109 84-85 -0.5509029447311988 2109 86-87 -0.39719961701657525 2109 88-89 0.02600204967093589 2109 90-91 -0.011325769536888686 2109 92-93 0.18721704745517798 2109 94-95 0.08793763777975983 2109 96-97 0.10978860473384344 2109 98-99 -0.415478856203336 2109 100-101 0.17990028975876982 2108 1 -0.053938688873845564 2108 2 -0.13717108882879003 2108 3 0.2621820820417362 2108 4 0.03199242149130299 2108 5 0.09777434449848244 2108 6 0.09980032247832327 2108 7 0.07718128121234713 2108 8 0.08026092196155332 2108 9 0.04228120920256373 2108 10-11 -0.023322044282565457 2108 12-13 0.036677364852558014 2108 14-15 -0.009325089563986921 2108 16-17 0.10148904701730288 2108 18-19 0.08140115946525839 2108 20-21 -0.057639666791665434 2108 22-23 0.232549565759804 2108 24-25 -0.1987745511372765 2108 26-27 0.020194801314168842 2108 28-29 0.12419552214963403 2108 30-31 0.1615559719280455 2108 32-33 0.5167239648526092 2108 34-35 0.0029447907101811666 2108 36-37 -0.1368377915406498 2108 38-39 0.20168333702265784 2108 40-41 -0.0781394331798353 2108 42-43 -0.07884462838454454 2108 44-45 0.30525136884802606 2108 46-47 0.44503283566505303 2108 48-49 0.05049151721594569 2108 50-51 -0.04423001294463802 2108 52-53 -0.4688334864646251 2108 54-55 0.1561385604700476 2108 56-57 0.10784426139861836 2108 58-59 -0.17422278891741882 2108 60-61 -0.23067131000899366 2108 62-63 -0.13472485672838985 2108 64-65 -0.4390091240150795 2108 66-67 -0.47500165774635406 2108 68-69 0.0316071814293295 2108 70-71 -0.023736885471357994 2108 72-73 0.2029497024470075 2108 74-75 0.032183346842145966 2108 76-77 -0.00233658982549656 2108 78-79 0.19550057682320698 2108 80-81 0.10148958714571421 2108 82-83 -0.46721163747133687 2108 84-85 -0.21696814744034754 2108 86-87 -0.17361931844779832 2108 88-89 0.07287949856070952 2108 90-91 -0.09567844417620819 2108 92-93 0.04573206573467559 2108 94-95 -0.01735956942601291 2108 96-97 -0.18355203670007825 2108 98-99 -0.2481150392780478 2108 100-101 -0.31895410971080906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 122.0 14 66.0 15 359.0 16 550.0 17 2654.0 18 11601.0 19 28877.0 20 40673.0 21 37915.0 22 39192.0 23 56257.0 24 86219.0 25 114396.0 26 146272.0 27 189936.0 28 241859.0 29 300117.0 30 374372.0 31 444623.0 32 508831.0 33 618860.0 34 810440.0 35 1159137.0 36 1956299.0 37 5200798.0 38 4340489.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 70.61387545887675 21.639941417926035 5.093294119041005 2.652889004156206 2 93.15626302666628 1.5740311990116158 1.1569325292440618 4.112773245078037 3 6.665661734600513 33.98383236249076 43.863429612527476 15.487076290381244 4 1.0224635841261336 22.339126533660966 75.5990471615764 1.0393627206365086 5 0.8874798829076614 96.78058902104337 1.104727126236183 1.2272039698127823 6 0.7575408502491247 1.2166300418995633 1.0086162851415548 97.01721282270975 7 0.8088067474944817 0.9828187734076066 1.017556550168351 97.19081792892956 8 0.7603473993104147 1.0103337256118965 97.08845967371983 1.1408592013578671 9 0.759916543164545 0.9977192151189337 97.2197451318342 1.02261910988232 10-11 48.71492067998196 1.0133347583501418 49.39217268427089 0.8795718773970113 12-13 0.8225731997663324 48.86059493813445 49.300358436408686 1.01647342569054 14-15 48.69531818233635 1.015079157701343 1.3994537162794842 48.89014894368282 16-17 0.8153234467007611 48.565596112815854 0.9588284638410561 49.660251976642336 18-19 0.7695749017677908 0.9428987546701515 97.23201555582179 1.0555107877402756 20-21 1.0370318463729753 27.327460963535565 35.32324742979348 36.31225976029797 22-23 10.566115653518413 9.189521291295017 13.695474705931703 66.54888834925487 24-25 1.1835528919469305 1.3774561157294003 38.27145356236809 59.16753742995557 26-27 39.25005239091052 25.89387091573806 7.028131435539672 27.827945257811752 28-29 0.7648235159369499 1.0485452800487156 53.15630252181299 45.03032868220134 30-31 7.182982331187869 42.7213346918068 32.871325889176376 17.22435708782895 32-33 35.900373253072814 49.3606154636425 8.378688921503635 6.36032236178105 34-35 18.86771124547706 34.68582269048838 34.13553262257229 12.310933441462268 36-37 7.978794577005184 32.1714808657384 25.228554225101036 34.62117033215538 38-39 12.700029453804863 52.888941323018 7.373325001852082 27.03770422132506 40-41 32.665879316954175 15.015710690630089 9.125066408695538 43.19334358372019 42-43 26.73347490149252 7.106580166710211 64.60166691061902 1.558278021178255 44-45 58.377985788210026 15.891829734747004 19.611024866742778 6.119159610300191 46-47 6.28111664029867 44.09398552347286 36.7208490211846 12.90404881504387 48-49 26.16703072016288 38.48523186703014 29.221453715817102 6.1262836969898835 50-51 27.621358712036937 20.032806105040095 25.860620191091883 26.485214991831086 52-53 7.463948411200009 2.4460511256296336 57.682990888469654 32.4070095747007 54-55 2.1692709327568798 8.402891667086552 42.78041883286575 46.64741856729081 56-57 15.033938749069584 13.739015524196208 15.958690708320644 55.26835501841356 58-59 21.027907270661554 7.249549605724738 32.31360056068747 39.40894256292624 60-61 19.76100469429739 45.94933287311514 18.075253095073077 16.214409337514393 62-63 44.158581032731064 19.246655209882597 19.120498136726695 17.474265620659647 64-65 12.945114791447073 19.677212150095443 51.54275223964411 15.83492081881338 66-67 17.76450707603426 35.79183640104904 17.12025446363975 29.323402059276948 68-69 26.865269009223553 32.05504797643026 9.558773984475057 31.520909029871135 70-71 12.87530412758991 31.846352030774618 29.952724309394448 25.325619532241024 72-73 15.612688809241673 26.153303164626422 17.376326633001643 40.857681393130264 74-75 17.51550513634383 46.96803538094924 24.64358921361213 10.872870269094797 76-77 13.612681508623645 17.89766256950398 33.1664743173234 35.32318160454898 78-79 13.303075463137445 19.05287466622113 31.07183125949903 36.57221861114239 80-81 28.19830201986558 20.236203118512847 33.89852284560856 17.66697201601301 82-83 27.604773742477523 13.962602524314349 37.21732096760237 21.21530276560576 84-85 26.645331907039914 22.573208742502057 23.450518625133252 27.330940725324776 86-87 5.869260053639196 50.98582878231556 18.201814095865732 24.943097068179515 88-89 8.252002501239684 17.302294695020713 6.739748248951202 67.7059545547884 90-91 10.985781507821775 35.674835020992866 12.489660948527412 40.84972252265794 92-93 11.069188076726384 16.853931508474044 33.37488302554845 38.70199738925112 94-95 11.515184028832893 13.368030019183868 17.2922857481045 57.824500203878735 96-97 16.79876097740674 28.170912734037167 20.393660095432242 34.63666619312385 98-99 35.7875697283823 14.603306557618573 34.51174184727418 15.097381866724943 100-101 31.91422384197537 17.401932653115203 37.61454340558512 13.06930009932431 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 177.0 1 101.5 2 18.0 3 8.0 4 5.5 5 3.5 6 2.0 7 1.5 8 2.5 9 4.5 10 5.5 11 4.5 12 7.0 13 14.0 14 18.5 15 30.0 16 65.5 17 90.5 18 110.0 19 150.0 20 240.5 21 353.5 22 568.5 23 1076.0 24 1553.5 25 2681.5 26 4132.5 27 10368.0 28 13458.5 29 16877.0 30 19658.0 31 16665.0 32 26344.5 33 85033.5 34 152129.5 35 155995.5 36 133341.0 37 380602.5 38 640824.5 39 456861.0 40 255276.5 41 214177.0 42 290113.0 43 583129.0 44 899363.0 45 817908.5 46 659144.5 47 665246.0 48 466979.0 49 452760.0 50 1495085.0 51 2949767.0 52 2202584.0 53 643815.0 54 575148.0 55 554158.5 56 249100.5 57 204128.5 58 173412.5 59 72402.0 60 40605.0 61 33555.0 62 29939.0 63 26364.5 64 18762.0 65 5446.5 66 2664.5 67 1664.0 68 1200.5 69 867.0 70 619.5 71 466.0 72 376.5 73 309.5 74 249.0 75 222.5 76 216.5 77 197.0 78 177.0 79 163.0 80 133.5 81 127.5 82 130.0 83 108.0 84 99.5 85 100.0 86 108.0 87 113.5 88 108.5 89 110.0 90 118.0 91 119.0 92 154.0 93 203.5 94 182.5 95 117.0 96 82.5 97 91.0 98 118.0 99 169.5 100 633.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 5.984113137079157E-6 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.9920565685395783E-6 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.9920565685395783E-6 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.9920565685395783E-6 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.9920565685395783E-6 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6710914E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 5.1716440153379155 #Duplication Level Percentage of deduplicated Percentage of total 1 86.33847044348155 4.465118339624609 2 5.949335376975563 0.6153568939514763 3 1.9031274879332511 0.2952689365018524 4 0.9281504104708663 0.19200254062580344 5 0.5159860897308757 0.1334248186477148 6 0.46990142294030546 0.14580977290488006 7 0.3122159204315439 0.11302687174751089 8 0.3199807773656371 0.1323861337828937 9 0.2549334334127815 0.11865824686968827 >10 2.2021399953433027 2.7675377303021755 >50 0.42924750693513847 1.4579680675501347 >100 0.2515802962012918 2.8013586656499774 >500 0.05191322232367642 1.9166169869897807 >1k 0.0547901674342446 5.876293679470159 >5k 0.008295826167233386 2.957452931347509 >10k+ 0.009931622852891248 76.01171938403381 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 2664526 15.944825040688979 No Hit AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 1426710 8.537594053802204 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC 1026752 6.1442001317222985 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT 886157 5.302863745214655 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA 717614 4.294283364751922 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT 623362 3.7302687333559374 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA 488947 2.925914166035443 No Hit TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG 471604 2.8221316918990786 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 448262 2.6824505230533773 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 284702 1.70368897835271 No Hit AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA 265086 1.5863046150557651 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT 211580 1.266118657543208 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA 187372 1.1212552467207957 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGTTAAGCTTTACATAT 182565 1.0924896148708563 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT 152657 0.9135167591670929 No Hit AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG 131827 0.788867682521734 No Hit CGGTACCTTGTTACGACTTTTACCGGGTTCAAGCCGCGGACGATCAGAAA 125894 0.7533639392794433 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA 119374 0.7143475216256873 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC 118836 0.7111280687579387 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTCGGTCAG 105090 0.6288704495756486 No Hit AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG 104382 0.6246336974745965 No Hit AGCAACCTTGTTACGACTTTTGCTTCTTCTAGCATGATAACATTGGATGG 95665 0.5724701832586775 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC 87817 0.5255068633588803 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC 63488 0.3799193748468815 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGTCAAGGTTTACTTAA 61270 0.36664661190883996 No Hit AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAAGTG 60044 0.3593100892027809 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA 58848 0.3521530898908342 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA 55207 0.330364933958729 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 53392 0.31950376861493035 No Hit AGCAACCTTGTTACGACTTCTACCATGTCAAGATCCCAAGTATCGTTACA 50242 0.300653812233131 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA 48148 0.28812307932408726 No Hit TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC 47329 0.2832220906648194 No Hit AGCTACCTTGTTACGACTTTTACCCGGTTCAAACCAATGCGATAAAAATG 42276 0.2529843669831584 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC 40992 0.24530076571514878 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT 40445 0.24202745582916652 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA 38373 0.2296283734091385 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT 37394 0.223769926647938 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 34437 0.2060749041015949 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA 32158 0.19243711026219154 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG 32056 0.19182673072220946 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC 31463 0.18827815163192152 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCCAAGGTTTGTTTAA 31226 0.18685991681843375 No Hit GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA 30963 0.18528609506338192 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 30878 0.1847774454467302 No Hit GGAAACCTTGTTACGCCTTATCCTTCCTCTAAGTGATATGGCTTACACAA 30746 0.18398754251263574 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC 30575 0.18296425916619521 No Hit GGAAACCTTGTTACGACTTCATCTTCCTCTAAATGTTAAGGTTTATTCAA 30006 0.17955929879119717 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAGATAA 29381 0.1758192280805227 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC 28731 0.17192955454142125 No Hit AGCAACCTTGTTACGACTTCTACCATGTCAAAATCCCAGGTATCGCTACA 28529 0.17072076368773126 No Hit GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTGACTAG 28277 0.16921276717718733 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA 24684 0.14771184867566192 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG 23807 0.1424637814544435 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTAAATCCACTGTGGAAGGT 23608 0.14127294294016474 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAAGC 23013 0.13771239562360263 No Hit GGAAACCTTGTTACGCCTTACCCTTCCTCTAAGTGATATGACTTACACAA 22100 0.13224890032944936 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA 21953 0.13136923569829873 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTACTTAA 19840 0.11872480463965046 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGC 19806 0.11852134479298979 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC 17904 0.10713956160626523 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.1542807293484963E-4 0.0 0.0 2 2.1542807293484963E-4 0.0 0.0 3 2.1542807293484963E-4 0.0 0.0 4 2.1542807293484963E-4 0.0 0.0 5 2.214121860719288E-4 0.0 0.0 6 2.2739629920900796E-4 0.0 0.0 7 2.3338041234608712E-4 0.0 0.0 8 2.3338041234608712E-4 0.0 0.0 9 2.3936452548316628E-4 0.0 0.0 10-11 2.3936452548316628E-4 0.0 0.0 12-13 2.4235658205170587E-4 0.0 0.0 14-15 2.4534863862024544E-4 0.0 0.0 16-17 2.5432480832586414E-4 0.0 0.0 18-19 2.633009780314829E-4 0.0 0.0 20-21 2.6928509116856203E-4 0.0 0.0 22-23 2.6928509116856203E-4 0.0 0.0 24-25 2.9621360028541825E-4 0.0 0.0 26-27 3.05189769991037E-4 0.0 0.0 28-29 3.171579962651953E-4 0.0 0.0 30-31 3.2314210940227446E-4 0.0 0.0 32-33 3.3511033567643273E-4 0.0 0.0 34-35 3.6503090136182857E-4 0.0 0.0 36-37 3.7999118420452646E-4 0.0 0.0 38-39 4.0392763675284305E-4 0.0 0.0 40-41 4.607767115550951E-4 0.0 0.0 42-43 4.996734469461096E-4 0.0 0.0 44-45 5.445542954742033E-4 0.0 0.0 46-47 6.193557096876928E-4 1.0771403646742482E-4 0.0 48-49 6.971491804697217E-4 1.0771403646742482E-4 0.0 50-51 8.048632169371466E-4 1.0771403646742482E-4 0.0 52-53 8.79664631150636E-4 1.0771403646742482E-4 0.0 54-55 9.873786676180608E-4 1.0771403646742482E-4 0.0 56-57 0.0010921006475169462 1.0771403646742482E-4 0.0 58-59 0.0012237511365326874 1.0771403646742482E-4 0.0 60-61 0.001370361908391127 1.0771403646742482E-4 0.0 62-63 0.0015648455853461995 1.0771403646742482E-4 0.0 64-65 0.0017922418845552073 1.0771403646742482E-4 0.0 66-67 0.002034598466606913 1.0771403646742482E-4 0.0 68-69 0.0023008915012069356 1.0771403646742482E-4 0.0 70-71 0.002570176592375498 1.0771403646742482E-4 0.0 72-73 0.002917255154326089 1.0771403646742482E-4 0.0 74-75 0.0032733098859822987 1.0771403646742482E-4 0.0 76-77 0.003599444051953113 1.0771403646742482E-4 0.0 78-79 0.0038926655956699916 1.0771403646742482E-4 0.0 80-81 0.004293601175854295 1.0771403646742482E-4 0.0 82-83 0.004772330226820627 1.0771403646742482E-4 0.0 84-85 0.005260035447492579 1.0771403646742482E-4 0.0 86-87 0.005738764498458912 1.0771403646742482E-4 0.0 88-89 0.0062474141151106395 1.0771403646742482E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTTGT 1577860 0.0 94.96056 5 CTTGTTA 1577120 0.0 94.95211 7 TGTTACG 1578465 0.0 94.950645 9 TTGTTAC 1578015 0.0 94.941 8 CCTTGTT 1581030 0.0 94.92098 6 GTTACCT 702080 0.0 94.885666 2 GATACCT 216305 0.0 94.857254 2 TACCTTG 1226345 0.0 94.85708 4 TCGTACC 54220 0.0 94.78974 1 AGATACC 187085 0.0 94.77149 1 AAACCTT 321440 0.0 94.63501 3 AACCTTG 352150 0.0 94.56972 4 GGAAACC 317700 0.0 94.53203 1 TTACCTT 706005 0.0 94.4658 3 GAAACCT 322450 0.0 94.37393 2 GGTTACC 697845 0.0 94.34792 1 ATACCTT 219660 0.0 94.31451 3 CTACCTT 215445 0.0 94.252594 3 CGTACCT 55790 0.0 94.17414 2 GTACCTT 95200 0.0 93.9073 3 >>END_MODULE