FastQCFastQC Report
Sun 19 Jul 2015
000000000-AGRBU l01n03.3330000000e59e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGRBU l01n03.3330000000e59e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16710914
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC266452615.944825040688979No Hit
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT14267108.537594053802204No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC10267526.1442001317222985No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT8861575.302863745214655No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA7176144.294283364751922No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT6233623.7302687333559374No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA4889472.925914166035443No Hit
TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG4716042.8221316918990786No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC4482622.6824505230533773No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC2847021.70368897835271No Hit
AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA2650861.5863046150557651No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT2115801.266118657543208No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA1873721.1212552467207957No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGTTAAGCTTTACATAT1825651.0924896148708563No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT1526570.9135167591670929No Hit
AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG1318270.788867682521734No Hit
CGGTACCTTGTTACGACTTTTACCGGGTTCAAGCCGCGGACGATCAGAAA1258940.7533639392794433No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA1193740.7143475216256873No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC1188360.7111280687579387No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTCGGTCAG1050900.6288704495756486No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG1043820.6246336974745965No Hit
AGCAACCTTGTTACGACTTTTGCTTCTTCTAGCATGATAACATTGGATGG956650.5724701832586775No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC878170.5255068633588803No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC634880.3799193748468815No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGTCAAGGTTTACTTAA612700.36664661190883996No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAAGTG600440.3593100892027809No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA588480.3521530898908342No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA552070.330364933958729No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC533920.31950376861493035No Hit
AGCAACCTTGTTACGACTTCTACCATGTCAAGATCCCAAGTATCGTTACA502420.300653812233131No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA481480.28812307932408726No Hit
TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC473290.2832220906648194No Hit
AGCTACCTTGTTACGACTTTTACCCGGTTCAAACCAATGCGATAAAAATG422760.2529843669831584No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC409920.24530076571514878No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT404450.24202745582916652No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA383730.2296283734091385No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT373940.223769926647938No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC344370.2060749041015949No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA321580.19243711026219154No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG320560.19182673072220946No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC314630.18827815163192152No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCCAAGGTTTGTTTAA312260.18685991681843375No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA309630.18528609506338192No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC308780.1847774454467302No Hit
GGAAACCTTGTTACGCCTTATCCTTCCTCTAAGTGATATGGCTTACACAA307460.18398754251263574No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC305750.18296425916619521No Hit
GGAAACCTTGTTACGACTTCATCTTCCTCTAAATGTTAAGGTTTATTCAA300060.17955929879119717No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAGATAA293810.1758192280805227No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC287310.17192955454142125No Hit
AGCAACCTTGTTACGACTTCTACCATGTCAAAATCCCAGGTATCGCTACA285290.17072076368773126No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTGACTAG282770.16921276717718733No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA246840.14771184867566192No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG238070.1424637814544435No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTAAATCCACTGTGGAAGGT236080.14127294294016474No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAAGC230130.13771239562360263No Hit
GGAAACCTTGTTACGCCTTACCCTTCCTCTAAGTGATATGACTTACACAA221000.13224890032944936No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA219530.13136923569829873No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTACTTAA198400.11872480463965046No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGC198060.11852134479298979No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC179040.10713956160626523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTGT15778600.094.960565
CTTGTTA15771200.094.952117
TGTTACG15784650.094.9506459
TTGTTAC15780150.094.9418
CCTTGTT15810300.094.920986
GTTACCT7020800.094.8856662
GATACCT2163050.094.8572542
TACCTTG12263450.094.857084
TCGTACC542200.094.789741
AGATACC1870850.094.771491
AAACCTT3214400.094.635013
AACCTTG3521500.094.569724
GGAAACC3177000.094.532031
TTACCTT7060050.094.46583
GAAACCT3224500.094.373932
GGTTACC6978450.094.347921
ATACCTT2196600.094.314513
CTACCTT2154450.094.2525943
CGTACCT557900.094.174142
GTACCTT952000.093.90733
Produced by FastQC (version 0.11.2)