##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AGRBU l01n02.3320000000e591.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16710914
Sequences flagged as poor quality	0
Sequence length	12
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.67705093808753	33.0	32.0	34.0	16.0	34.0
2	31.01261307430581	33.0	32.0	34.0	16.0	35.0
3	31.317211673760035	33.0	32.0	34.0	18.0	35.0
4	31.068019080225056	33.0	32.0	34.0	16.0	35.0
5	30.980472761693346	33.0	32.0	34.0	16.0	35.0
6	32.73059702180264	35.0	33.0	37.0	18.0	37.0
7	32.7122820451353	34.0	33.0	37.0	18.0	37.0
8	32.67251904952656	35.0	33.0	37.0	18.0	37.0
9	32.43313004902066	34.0	32.0	37.0	16.0	37.0
10	32.60282728999742	34.0	33.0	37.0	18.0	37.0
11	33.11940519830333	35.0	33.0	37.0	18.0	37.0
12	32.89605577528554	36.0	33.0	37.0	18.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.3350528827385162
1101	2	0.3188798857601718
1101	3	0.3026400490769241
1101	4	0.49861260168546906
1101	5	0.5808095338838655
1101	6	0.6899237879946796
1101	7	0.640099894798702
1101	8	0.8888379161623732
1101	9	0.7638403632173407
1101	10	0.7297712406966141
1101	11	0.5993103654821823
1101	12	0.6781808830274016
1102	1	0.46699329682469326
1102	2	0.5710348890404262
1102	3	0.4324363420509947
1102	4	0.5067942174893467
1102	5	0.7543508496020266
1102	6	-0.6245869390101575
1102	7	0.34609145550813736
1102	8	0.5729843289277028
1102	9	0.5602954137196008
1102	10	0.6561605097484602
1102	11	0.6039879182418844
1102	12	0.027658215861237068
1103	1	0.6133851873052052
1103	2	0.5274861218171552
1103	3	0.4924899987535767
1103	4	0.7084715169740115
1103	5	0.7340300843143339
1103	6	0.7194015635982893
1103	7	0.7826405897154913
1103	8	0.9085272003288694
1103	9	0.728334211657959
1103	10	0.69492063347748
1103	11	0.5339069612896381
1103	12	0.6277741524017131
2112	1	0.5335343727162289
2112	2	0.4889561037265082
2112	3	0.48262544042583144
2112	4	0.6734067899614367
2112	5	0.6886100619250541
2112	6	0.559687099511919
2112	7	0.7102665577233012
2112	8	0.9272138389510332
2112	9	0.7208846914959679
2112	10	0.7214045664585171
2112	11	0.4399717829314582
2112	12	0.6810819616967194
1113	1	-6.771082222542397
1113	2	-4.451509117387808
1113	3	-2.499990132632629
1113	4	-7.342973030608388
1113	5	-8.885966812495791
1113	6	-2.742397556592163
1113	7	-9.055680554434456
1113	8	-8.784529911859405
1113	9	-10.503126529051961
1113	10	-10.04602644855267
1113	11	-6.903035770541415
1113	12	-9.488123495513694
2113	1	0.46589106035654737
2113	2	0.4681316527461341
2113	3	0.3783494664359921
2113	4	0.5664123353825943
2113	5	0.6259793813083299
2113	6	0.002806652694573586
2113	7	0.5110820648023733
2113	8	0.5736281338144593
2113	9	0.62739262752212
2113	10	0.6016557781176957
2113	11	0.5789596719043217
2113	12	0.40584260610278733
1112	1	0.7082914202646009
1112	2	0.5333584576520352
1112	3	0.3347857618142669
1112	4	0.4972911321257527
1112	5	0.6146446316782956
1112	6	0.4920980791960403
1112	7	0.37802052085240234
1112	8	0.7152339287580531
1112	9	0.6647758202233263
1112	10	0.5569864863766725
1112	11	0.5031449343942995
1112	12	0.6537159010028475
2114	1	0.5667061211339579
2114	2	0.47917721420527926
2114	3	0.27103073586794935
2114	4	0.48124314149939096
2114	5	0.5323153632682001
2114	6	-0.021922441945278592
2114	7	0.3386278626397754
2114	8	0.6534706436185047
2114	9	0.6217422595709721
2114	10	0.6120976102640014
2114	11	0.381357977000647
2114	12	0.6922362292039423
1114	1	0.7322632006959005
1114	2	0.47084007984800635
1114	3	0.31165201029591216
1114	4	0.5743060738992831
1114	5	0.5991900524862146
1114	6	-0.28459173457266473
1114	7	0.32104946932714995
1114	8	0.7261642127111969
1114	9	0.5386836819607126
1114	10	0.3764089855266448
1114	11	0.4025603699312512
1114	12	0.5524995976122398
1109	1	0.6023343656649054
1109	2	0.39396233004098846
1109	3	0.2932666698778128
1109	4	0.4502494761829112
1109	5	0.5058271712005826
1109	6	-0.6003779509548224
1109	7	0.1757886993893294
1109	8	0.6064958783416472
1109	9	0.5964228120099122
1109	10	0.6144150133959343
1109	11	0.3638899792851191
1109	12	0.6116934364523559
1108	1	0.43909460693131663
1108	2	0.25571033780708774
1108	3	0.199939361345443
1108	4	0.2955516072880826
1108	5	0.47191454201872673
1108	6	0.38926224151053646
1108	7	0.4563007982403704
1108	8	0.5043692667844439
1108	9	0.45459731567377304
1108	10	0.4241033265504939
1108	11	0.33566643610321734
1108	12	0.46724316816096234
1111	1	0.4730648423804453
1111	2	0.0841522971477815
1111	3	0.132674726332624
1111	4	0.1696937162635983
1111	5	0.319581821418236
1111	6	0.1851602375833039
1111	7	0.3276725003612597
1111	8	0.5918984891014603
1111	9	0.4359735608051736
1111	10	0.35567024233956346
1111	11	0.3464791887625509
1111	12	0.20029571906921007
1110	1	0.5538745991095269
1110	2	0.24041506036449078
1110	3	0.09465766924586205
1110	4	0.35708687842939213
1110	5	0.3349808992965606
1110	6	0.292854996768213
1110	7	0.3536725894377355
1110	8	0.6182258530959572
1110	9	0.5620623532279652
1110	10	0.4004273654001764
1110	11	0.2921866953235934
1110	12	0.4777202482256442
1105	1	0.4599436206465093
1105	2	0.28575780307576437
1105	3	0.14388880651298663
1105	4	0.04434384736447683
1105	5	0.3181425757097358
1105	6	0.6147285377336544
1105	7	0.42336127473434004
1105	8	0.5022910817630688
1105	9	0.4829087859526524
1105	10	0.2031329348273303
1105	11	0.16495154864789185
1105	12	0.16135476139709937
1104	1	-0.8204784077532814
1104	2	-0.5125920431768058
1104	3	-0.3008575098689583
1104	4	0.10501328201119264
1104	5	0.1197130128838495
1104	6	0.5504108293489551
1104	7	0.49282755349688045
1104	8	-4.49131590287336
1104	9	-0.7052581204562856
1104	10	-0.15966248220539825
1104	11	0.20472144645263768
1104	12	0.40754103109231465
1107	1	0.036516472283867785
1107	2	0.13847034773709055
1107	3	0.010829060019030834
1107	4	0.3610109541054918
1107	5	0.3479804179433721
1107	6	0.4005144966668581
1107	7	0.4725351033879974
1107	8	0.5806104116578581
1107	9	0.28438169078760467
1107	10	0.4365281252321296
1107	11	0.33946438544042934
1107	12	0.32497480686275537
1106	1	0.2123722215586703
1106	2	0.1044709537292583
1106	3	0.15768522100984583
1106	4	0.17479983260038878
1106	5	0.2617198451146834
1106	6	0.3650378849487126
1106	7	0.4520281040901821
1106	8	0.5658830336671343
1106	9	0.4594950178313866
1106	10	0.49295050931181095
1106	11	0.303052236247936
1106	12	0.44971567601153595
2103	1	0.26325229521484417
2103	2	0.24322396396231127
2103	3	0.1287356563474198
2103	4	0.16969264291639163
2103	5	0.23114627929593468
2103	6	0.21520822241060955
2103	7	0.4433520730307521
2103	8	0.2673126487334443
2103	9	0.34113080176960153
2103	10	0.48307358911947773
2103	11	0.22032619745817783
2103	12	0.7121745186306185
2102	1	-0.6499670872421319
2102	2	-0.46448664363811787
2102	3	-0.43606776344945786
2102	4	-0.21311256828508718
2102	5	-0.2857980755560483
2102	6	-0.40628731252801487
2102	7	0.1357815772959654
2102	8	0.05004132782777759
2102	9	-0.05061200302346691
2102	10	-0.22543577674809256
2102	11	-0.3140332036627669
2102	12	-0.3005041629304799
2101	1	0.28969495325697636
2101	2	0.2833909294874033
2101	3	0.21611252181491025
2101	4	0.43222439942922364
2101	5	0.5394043615234239
2101	6	0.1499080938896853
2101	7	0.45110203862437004
2101	8	0.6108729760303362
2101	9	0.5010512606025941
2101	10	0.5600726823942068
2101	11	0.3512435216230472
2101	12	0.5260098970068015
2107	1	0.18260163200189794
2107	2	-0.006027721587795298
2107	3	-0.1642603536904943
2107	4	0.12623258820518757
2107	5	0.14991639059786266
2107	6	0.27644319762010383
2107	7	0.15933526493938643
2107	8	0.25596002811494856
2107	9	0.38837453523565557
2107	10	0.3483225005656436
2107	11	0.20784139924963085
2107	12	0.27321048492297706
2106	1	0.007213872811956179
2106	2	0.011449096975852058
2106	3	0.0645486192013287
2106	4	0.17205082958105677
2106	5	-0.0498768981566009
2106	6	0.15049605553648604
2106	7	0.3575424102887155
2106	8	0.42829448615879784
2106	9	0.38340120459235294
2106	10	0.22427278319180743
2106	11	0.1908808008766556
2106	12	0.18541929575633276
2105	1	0.22421683571883477
2105	2	0.06210565861127293
2105	3	-0.1200081056077984
2105	4	0.17822082171659304
2105	5	0.2126909889539732
2105	6	0.04101471496154119
2105	7	-0.5413397956526182
2105	8	0.21429983245202067
2105	9	0.1773425658308554
2105	10	0.31520322718858296
2105	11	-0.07556582139108059
2105	12	0.2619721946883615
2104	1	-0.026745859385584936
2104	2	-0.2121306406525747
2104	3	-0.1911554226868688
2104	4	0.11175427099678004
2104	5	0.056883334077845404
2104	6	-2.8895191297618155
2104	7	-0.2127467497244666
2104	8	0.15940719002532688
2104	9	0.008436246371473999
2104	10	-0.0026027338459400085
2104	11	0.06692553462678319
2104	12	0.28772738174472323
2111	1	0.03086065772581037
2111	2	0.07222482022581644
2111	3	-0.08633588274959081
2111	4	-0.12943193195030034
2111	5	0.1749086502504369
2111	6	0.26548553386543006
2111	7	0.2695367191624385
2111	8	0.35638011493720967
2111	9	0.25738722898088895
2111	10	0.27266788646350903
2111	11	-0.08831455825286838
2111	12	0.20875790379740522
2110	1	0.1274871364712169
2110	2	-0.12965440733484002
2110	3	-0.03161428415279843
2110	4	0.013592765448098731
2110	5	0.12564981103613704
2110	6	0.4605564012964649
2110	7	0.32086826411805447
2110	8	0.5404518144661807
2110	9	0.3288914306973041
2110	10	0.06985082773445583
2110	11	0.1340432185970215
2110	12	0.2879048970654736
2109	1	0.06047786215103912
2109	2	0.060502283788007816
2109	3	-0.24309404798842849
2109	4	0.017581218978200752
2109	5	0.057880068396702455
2109	6	0.3443497685235073
2109	7	0.22816074811522924
2109	8	0.18150425721168517
2109	9	0.1492730162560889
2109	10	0.16769876368521608
2109	11	-0.1642372956582605
2109	12	-0.35716043664341157
2108	1	-0.116849939040371
2108	2	-0.31729971397078316
2108	3	-0.37496461360159117
2108	4	-1.1940969063672924E-4
2108	5	-0.136628341975765
2108	6	0.40433466970542753
2108	7	0.26202296573102046
2108	8	0.27548692109133555
2108	9	0.221917756538609
2108	10	0.11593185328554512
2108	11	-0.019685920363819776
2108	12	-0.0169168727058846
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	41.0
15	2222.0
16	58406.0
17	127695.0
18	197917.0
19	270154.0
20	274575.0
21	315791.0
22	335202.0
23	332391.0
24	315558.0
25	310407.0
26	294898.0
27	287313.0
28	295377.0
29	316702.0
30	369454.0
31	490362.0
32	814397.0
33	2049320.0
34	4386601.0
35	4227940.0
36	638191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.21037197606307	18.153315850946274	26.182188478739103	27.454123694251553
2	21.858122182903937	23.143425907164623	22.097265296201034	32.9011866137304
3	21.287243773739725	39.47548290895399	12.835234506024026	26.402038811282257
4	33.28850833652785	11.211738627821315	29.106834012789484	26.392919022861346
5	29.78936998897846	20.399518542193444	25.33444310706165	24.47666836176645
6	33.91757626183703	4.707600075016842	21.09803210045842	40.27679156268771
7	14.672315350315369	34.535980497535924	41.724216880058144	9.067487272090563
8	22.772823796472174	23.039350211484543	15.651747115687389	38.536078876355894
9	26.17535618789949	18.769770388966776	29.70361942522231	25.351253997911428
10	29.71505927204221	26.137217868513957	37.928308409701586	6.2194144497422466
11	13.919938789703542	19.263081600443876	39.43938673851113	27.37759287134145
12	22.63474772443612	16.626410537832374	33.927051143166146	26.81179059456536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1170.0
1	1170.0
2	1170.0
3	1170.0
4	3373.0
5	5576.0
6	5576.0
7	5576.0
8	5576.0
9	5576.0
10	5576.0
11	5576.0
12	5576.0
13	13554.0
14	21532.0
15	21532.0
16	21532.0
17	21532.0
18	21532.0
19	21532.0
20	21532.0
21	44293.5
22	67055.0
23	67055.0
24	67055.0
25	67055.0
26	67055.0
27	67055.0
28	67055.0
29	159526.5
30	251998.0
31	251998.0
32	251998.0
33	251998.0
34	251998.0
35	251998.0
36	251998.0
37	251998.0
38	2293602.5
39	4335207.0
40	4335207.0
41	4335207.0
42	4335207.0
43	4335207.0
44	4335207.0
45	4335207.0
46	5008618.0
47	5682029.0
48	5682029.0
49	5682029.0
50	5682029.0
51	5682029.0
52	5682029.0
53	5682029.0
54	5852244.0
55	6022459.0
56	6022459.0
57	6022459.0
58	6022459.0
59	6022459.0
60	6022459.0
61	6022459.0
62	6022459.0
63	3142372.0
64	262285.0
65	262285.0
66	262285.0
67	262285.0
68	262285.0
69	262285.0
70	262285.0
71	155183.0
72	48081.0
73	48081.0
74	48081.0
75	48081.0
76	48081.0
77	48081.0
78	48081.0
79	28867.5
80	9654.0
81	9654.0
82	9654.0
83	9654.0
84	9654.0
85	9654.0
86	9654.0
87	9654.0
88	6175.5
89	2697.0
90	2697.0
91	2697.0
92	2697.0
93	2697.0
94	2697.0
95	2697.0
96	1934.0
97	1171.0
98	1171.0
99	1171.0
100	1171.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.984113137079157E-6
10	0.0
11	0.0
12	5.984113137079157E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	1.6710914E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.2742596786930824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26307306674718	1.6434499333415522
2	10.122399430551281	0.4604192975305719
3	4.2575541138009925	0.2904835095261437
4	2.60527554699068	0.23700292513623875
5	1.6109196796528182	0.1831824836523791
6	1.167570658324694	0.1593215322151794
7	1.022389086589928	0.1627624792897127
8	0.7514305907327828	0.13671586350720738
9	0.5977226277768017	0.12234388242557287
>10	4.415738303670809	2.0295547758810666
>50	0.5696264308881823	0.9038430215858078
>100	0.43947488938325363	2.0514754097152776
>500	0.08668358553366011	1.4173194004894574
>1k	0.08269858606500315	3.409121291128419
>5k	0.0013291791592658748	0.1991606492328676
>10k+	0.006114224132623015	86.59384354534254
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTCGTTCGACT	990059	5.924625068383453	No Hit
TTACTGTGCGAT	840954	5.032363879079265	No Hit
TCCGACACAATT	835089	4.997267055530296	No Hit
ATGTGCACGACT	822559	4.9222861179226935	No Hit
TACACGATCTAC	817919	4.894519832966647	No Hit
GCACACACGTTA	786844	4.708563517231912	No Hit
CTAACCTCCGCT	779557	4.664957284802016	No Hit
CCAGTGTATGCA	773555	4.629040637753267	No Hit
GCTGATGAGCTG	754692	4.516162311648543	No Hit
GTATGCGCTGTA	689806	4.127877146636025	No Hit
GGATGGTGTTGC	685805	4.10393470997457	No Hit
AGCTGTTGTTTG	685625	4.102857569609897	No Hit
CACGCCATAATG	684404	4.095550967469523	No Hit
CAGGCGTATTGG	645460	3.8625056654591123	No Hit
GACTTGGTATTC	638475	3.820706635196615	No Hit
TGAGTCACTGGT	625095	3.740639201422496	No Hit
AGGCTACACGAC	590260	3.532182620292343	No Hit
TAGGATTGCTCG	569904	3.4103700132739596	No Hit
GGATCGCAGATC	537114	3.2141509435091344	No Hit
ACGCGCAGATAC	534374	3.1977544735135375	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTGGC	1250	0.0	6.0000005	6
GACGGTG	245	0.0	6.0000005	2
CCAGTCT	425	0.0	6.0000005	1
CCCTGCG	55	0.009160799	6.0	4
TCCGGCA	75	1.7513952E-4	6.0	1
CTTAATG	240	0.0	6.0	6
GTCATTA	60	0.0033966054	6.0	4
ATCCTGC	55	0.009160799	6.0	3
GCCCCAA	65	0.0012619871	6.0	4
GTCATAG	60	0.0033966054	6.0	4
GTCATAA	200	0.0	6.0	4
TATCAAG	65	0.0012619871	6.0	3
AACGTCC	195	0.0	6.0	3
GGGTGGT	80	6.5395114E-5	6.0	1
GGGTGAT	75	1.7513952E-4	6.0	3
TGATAAG	165	3.6379788E-12	6.0	3
GTCGCGA	90	9.152645E-6	6.0	5
TTGGCAT	865	0.0	6.0	4
TCCATAA	545	0.0	6.0	4
TCGCAGG	210	0.0	6.0	4
>>END_MODULE