FastQCFastQC Report
Sun 19 Jul 2015
000000000-AGRBU l01n02.3320000000e591.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGRBU l01n02.3320000000e591.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16710914
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGTTCGACT9900595.924625068383453No Hit
TTACTGTGCGAT8409545.032363879079265No Hit
TCCGACACAATT8350894.997267055530296No Hit
ATGTGCACGACT8225594.9222861179226935No Hit
TACACGATCTAC8179194.894519832966647No Hit
GCACACACGTTA7868444.708563517231912No Hit
CTAACCTCCGCT7795574.664957284802016No Hit
CCAGTGTATGCA7735554.629040637753267No Hit
GCTGATGAGCTG7546924.516162311648543No Hit
GTATGCGCTGTA6898064.127877146636025No Hit
GGATGGTGTTGC6858054.10393470997457No Hit
AGCTGTTGTTTG6856254.102857569609897No Hit
CACGCCATAATG6844044.095550967469523No Hit
CAGGCGTATTGG6454603.8625056654591123No Hit
GACTTGGTATTC6384753.820706635196615No Hit
TGAGTCACTGGT6250953.740639201422496No Hit
AGGCTACACGAC5902603.532182620292343No Hit
TAGGATTGCTCG5699043.4103700132739596No Hit
GGATCGCAGATC5371143.2141509435091344No Hit
ACGCGCAGATAC5343743.1977544735135375No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGC12500.06.00000056
GACGGTG2450.06.00000052
CCAGTCT4250.06.00000051
CCCTGCG550.0091607996.04
TCCGGCA751.7513952E-46.01
CTTAATG2400.06.06
GTCATTA600.00339660546.04
ATCCTGC550.0091607996.03
GCCCCAA650.00126198716.04
GTCATAG600.00339660546.04
GTCATAA2000.06.04
TATCAAG650.00126198716.03
AACGTCC1950.06.03
GGGTGGT806.5395114E-56.01
GGGTGAT751.7513952E-46.03
TGATAAG1653.6379788E-126.03
GTCGCGA909.152645E-66.05
TTGGCAT8650.06.04
TCCATAA5450.06.04
TCGCAGG2100.06.04
Produced by FastQC (version 0.11.2)