FastQCFastQC Report
Sun 19 Jul 2015
000000000-AGRBU l01n01.3310000000e594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGRBU l01n01.3310000000e594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16710914
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG232777013.929639037098749No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG11274686.746896070436363No Hit
ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC6937254.151328886020238No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC6619083.9609323583377902No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG5349573.2012432114724545No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGTGTCACCGCT5194753.108597171884195No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA5013873.000356533460707No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG4603742.75493010136968No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG3501032.095055961630824No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC3393192.0305232855605624No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTGCAAAGCT3227321.9312648009558304No Hit
GCTATCCGGGACTGAGTTGTTTCGAGAGGAGTGGAGACTGTTGTTTTGGG2887501.7279126683316066No Hit
GCTCCTACCGATACCGGGTGTTGTGCTGAACCTATTAGACCACTTGTGGA1879811.1248995716212769No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA1874681.1218297215819553No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCATAA1730391.0354849531270403No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG1666310.997138756144637No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT1652130.9886532837162587No Hit
ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG1524480.9122660795214433No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACTAGCAGACGTTTT1515580.9069402188294428No Hit
GCTACCGGGGACTCTGAGCTGCGGCGAGAAGAATTTTTTTCTGATCGTCC1366510.8177350442950038No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG1351710.8088785568521267No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTTTTCTAAA1346250.8056112310792815No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC1221120.73073202339501No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTAATAATAGG1208720.7233117231050319No Hit
ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG1160860.694671757630971No Hit
GCTACTACTGATGAATTGTTTAGTGAGGTCTCTGGACGTGTACAAGATGA1118660.6694188001924969No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG1088430.6513288261791067No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGACAGGAC985030.5894530963417082No Hit
GTTGCTACTGATGGTATGGTAACAGAGGAAGGAGGAGAAACGTTAGTTAC980960.587017562294917No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG931820.5576116303393099No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG854160.5111390077167532No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCGGTAGCAATA747790.44748599627764224No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG698060.41772700164694765No Hit
GCTGTCCGGGACTGAGCTGTTTCGAGAAGAGTGAGGACTGCTGCAGCGAG659970.39493351470781307No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTGCGGAG613720.367256991448822No Hit
GCTATTACCGATTGAGTGTTAAGGTGAACCTTCTCGATAGAGCAATCTAA610060.365066806040651No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTG583950.34944228663973737No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC558270.3340750841037181No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTGTCATGT504440.301862603086821No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACGGCATGTG498880.298535436182605No Hit
GCTATCCGGGACTGAACTGATTCGAGAAGAGTGGGGACTGTCGCTTCGAG477520.28575337052180394No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA476160.28493953113516113No Hit
ACACCATGGGAGTGGAGGATACCAGAAGTAGCTAGACTAACCGCAAGGGG459700.27508968091152886No Hit
GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGCGATTGGG444600.2660536700745393No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG442820.2649884979361392No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT438280.2622717105719053No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACTGATGCATTAGG391800.23445755271076135No Hit
ACACCATGGGAGTTGAATTCACCCGAAGCCGGAATACTAAACTAGTTACC341110.20412408321890713No Hit
GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGCAAACTAG328360.1964943389691312No Hit
GCTTTTACCGATTGAGTGTCCAGGTGAGTCTTCTTGATAGTTGCAAAATT323100.19334669545902755No Hit
GCTACCGCGAAAGGGTCCTAAGTAAAGCTTCACGGAGTGGCCGCACGGGG322790.1931611879517781No Hit
ACTTCTACCGATTCGAGTGGTTCGGCGAATGCTTCGGATCGACCCGCAAG317810.19018109960951268No Hit
ACTACTACCGATTGAATGGCTTAGTGAGGCTTCCGGATTGGTTTAAAGAA313300.18748226458468997No Hit
ACACCATGGGAGCGGGTTCCGCCAGAAGTAGGTAGCCTAACCGCAAGGGG307480.1839995107389099No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGGCGTTTC301730.1805586456850894No Hit
GTTGTTTCCGATGGTTGGCGAAATACAGGTTGTTGGACTGGCTTCGTGCC292870.17525672144563725No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA284580.17029589165499864No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTATGTG279320.167148248144895No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGTCGCAAGACT262860.1572983979212627No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTCCGTGCC250100.1496626695583497No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGTCGCAAGATT247770.14826837119741026No Hit
GCTTGTAGTAACGAATGATCTGGTGAACCTTCTGGACTGCTTCTGAATAA245980.1471972149458731No Hit
GCTACCGCGAAGAGGTCTCATGTAAAGCTCCACGGAGTTGGCGCTTGGTT244080.14606023344982805No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGCCGCAAGGCT236680.14163198972838947No Hit
GCTATTACCGATTGAGTGTATAGGTGAACCTTCCTGATAGCTTTCGGGCT235520.14093783260448828No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTGTCAAAGCT229970.13761664981340938No Hit
ACTCCTACCGATTCGAGTGGTTCGACGAATTCTCCGGATATCGTTCCCAG225560.13497765591995747No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGTAAGGAG218760.13090845898674364No Hit
GCTGCTACCGATTGAATAGTAAATGTGAAATGGGAGGACTTTGCTGTCTG218400.1306930309138088No Hit
ACACCATGGGAGCGGGTTCTGCCAGAAGTGGGTAGCCTAACCGCAAGGAG214200.12817970339623555No Hit
GCTGCTACCGATTGAATAGGAAATGTGAAATGGGAGGACGGGAGACTCTG209370.1252893767510263No Hit
GTTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATGCGAATTCACTT209060.12510386924377684No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGGCGCAAGCTT206170.12337446054716099No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGGGGAATTT205320.12286581093050924No Hit
GCACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG204240.12221952671170469No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCTTAGGGAG199610.11944888232923706No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTTAATGGATT197400.11812639332594256No Hit
GCTACCGCGAAAGGGTCCTAAGTAAAGCTTTACGGAGTGACAGTATGGGG197160.11798277461065267No Hit
GCACCTACCGATTGAATGATTCGGTGAAGATTCTGGACCGTGATCGATCG195650.11707917352695371No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCCTAACCTTCGGGAG194330.11628927059285925No Hit
GCTACTACCGATCGAATGGCTTAGTGAGGCTTCCGGATTGTTGATGCGGA193270.11565495460032886No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGCCTAACCTTCGGGAG185170.11080782295929476No Hit
GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGTAAACTAA184300.11028720511636884No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGGTAGTCTAACCTTCGGGAG175310.1049074874061347No Hit
GCTGCTACCGATTGAATAGTAAATGTGAAATGGGAGGACTCTTGTCTCTT171030.10234628698346483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC156.6195085E-495.03
CCGAGTC750.095.08
CCATGGG8235850.094.986734
GCTATCC971500.094.980441
CACCATG8234550.094.959622
GCTATTA1035200.094.944951
TATCCGG973800.094.941473
CCGGGAC1052850.094.932336
ACCATGG8225700.094.929553
ACACCAT8166900.094.927881
CTGTCCG201000.094.905472
TTGTAGT1674400.094.903553
ATCCGGG974950.094.902564
CTATCCG974800.094.8976752
CCGATTG2949100.094.880818
CTTGTAG1680150.094.861472
AGTAACG1679550.094.861437
CATGGGA8246550.094.853125
TACTACC252850.094.8497243
CTACTAC353200.094.8386152
Produced by FastQC (version 0.11.2)