FastQCFastQC Report
Thu 2 Jul 2015
000000000-AGE3A l01n03.3330000000daa9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGE3A l01n03.3330000000daa9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16049282
Sequences flagged as poor quality0
Sequence length301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAANNATCTTTGGCAAATGCTTNCGNATTAGTTAGNCCTTAATAAATC192680.12005521493111031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAC146000.01107.15331
TGAAGAC29500.0748.52491
TGAACAC59650.0591.851261
CGAAGAC28500.0591.530461
TCCTTGG339500.0551.407969
ATTCTTG179800.0544.06328
ATCCTTG323500.0534.92818
TCTTTGG121250.0514.264479
TCGTTGG22500.0510.179329
TGATGGG3250.0508.555631
TTCTTGG198550.0504.47219
ATCTTTG120800.0504.417368
TGAAGAT7050.0497.013951
GCTTTCG405150.0479.5203220-24
TGCTTTC376450.0476.8239420-24
TGAAAGC3450.0459.911131
TGAAAAT41750.0457.639161
TCCATGG8450.0441.83119
TAATTGG2550.0437.046849
GTAATCG6250.0427.956278
Produced by FastQC (version 0.11.2)