##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AGE3A l01n03.3330000000daa9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16049282
Sequences flagged as poor quality	0
Sequence length	301
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.84302157566924	31.0	21.0	33.0	12.0	34.0
2	25.22451328352259	31.0	19.0	34.0	11.0	34.0
3	17.303353882123822	19.0	2.0	32.0	2.0	34.0
4	10.592083558628978	2.0	2.0	23.0	2.0	34.0
5	17.3993260259244	23.0	11.0	23.0	2.0	33.0
6	8.688316399450144	2.0	2.0	11.0	2.0	30.0
7	2.693976216506134	2.0	2.0	2.0	2.0	2.0
8	11.69995679557503	2.0	2.0	25.0	2.0	28.0
9	18.383969014937865	25.0	11.0	28.0	2.0	28.0
10-14	15.537133598873769	14.8	7.2	22.2	3.8	29.4
15-19	14.889094689718828	15.6	7.6	21.0	3.8	23.0
20-24	14.98304645653307	13.2	5.6	22.2	2.0	28.6
25-29	9.695453915010031	9.6	2.0	15.8	2.0	17.4
30-34	20.41539311229001	24.2	9.2	29.8	2.0	33.6
35-39	13.285453367945058	11.2	5.4	20.2	3.8	26.8
40-44	18.394926850933267	18.6	5.6	27.6	3.8	33.2
45-49	21.06472427863128	24.2	7.4	34.2	3.8	37.0
50-54	11.385166900301211	10.8	5.6	15.6	3.8	17.2
55-59	11.916606724213583	12.4	2.0	19.2	2.0	23.6
60-64	9.161484183529206	8.2	2.0	11.2	2.0	17.6
65-69	14.145050501324608	14.0	5.6	19.8	2.0	31.6
70-74	21.07896325829405	23.0	11.0	30.6	5.6	35.4
75-79	20.01173787089042	17.8	7.4	34.0	3.8	37.0
80-84	22.038279456987546	23.8	9.0	33.4	7.4	37.0
85-89	10.288675942014104	6.6	2.0	16.4	2.0	25.2
90-94	20.47329834443684	23.4	9.0	29.0	5.4	35.6
95-99	20.03849927990548	21.8	2.0	34.0	2.0	37.0
100-104	22.112037398308537	24.0	12.0	33.6	5.6	37.0
105-109	22.139214987935286	22.2	11.6	33.4	7.0	37.0
110-114	21.56996754122708	24.2	9.2	33.8	5.2	37.2
115-119	21.13385838693594	20.4	11.0	31.0	10.0	36.8
120-124	21.71662166569196	23.4	7.4	33.4	7.2	37.0
125-129	19.466500769317907	20.6	3.6	33.2	2.0	37.0
130-134	2.0	2.0	2.0	2.0	2.0	2.0
135-139	2.0	2.0	2.0	2.0	2.0	2.0
140-144	2.0	2.0	2.0	2.0	2.0	2.0
145-149	2.0	2.0	2.0	2.0	2.0	2.0
150-154	2.0	2.0	2.0	2.0	2.0	2.0
155-159	2.0	2.0	2.0	2.0	2.0	2.0
160-164	2.0	2.0	2.0	2.0	2.0	2.0
165-169	2.0	2.0	2.0	2.0	2.0	2.0
170-174	2.0	2.0	2.0	2.0	2.0	2.0
175-179	2.0	2.0	2.0	2.0	2.0	2.0
180-184	2.0	2.0	2.0	2.0	2.0	2.0
185-189	2.0	2.0	2.0	2.0	2.0	2.0
190-194	2.0	2.0	2.0	2.0	2.0	2.0
195-199	2.0	2.0	2.0	2.0	2.0	2.0
200-204	2.0	2.0	2.0	2.0	2.0	2.0
205-209	2.0	2.0	2.0	2.0	2.0	2.0
210-214	2.0	2.0	2.0	2.0	2.0	2.0
215-219	2.0	2.0	2.0	2.0	2.0	2.0
220-224	2.0	2.0	2.0	2.0	2.0	2.0
225-229	2.0	2.0	2.0	2.0	2.0	2.0
230-234	2.0	2.0	2.0	2.0	2.0	2.0
235-239	2.0	2.0	2.0	2.0	2.0	2.0
240-244	2.0	2.0	2.0	2.0	2.0	2.0
245-249	2.0	2.0	2.0	2.0	2.0	2.0
250-254	2.0	2.0	2.0	2.0	2.0	2.0
255-259	2.0	2.0	2.0	2.0	2.0	2.0
260-264	2.0	2.0	2.0	2.0	2.0	2.0
265-269	2.0	2.0	2.0	2.0	2.0	2.0
270-274	2.0	2.0	2.0	2.0	2.0	2.0
275-279	2.0	2.0	2.0	2.0	2.0	2.0
280-284	2.0	2.0	2.0	2.0	2.0	2.0
285-289	2.0	2.0	2.0	2.0	2.0	2.0
290-294	2.0	2.0	2.0	2.0	2.0	2.0
295-299	2.0	2.0	2.0	2.0	2.0	2.0
300-301	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6157194829911958
1101	2	1.8395248589491366
1101	3	8.809456583328291
1101	4	17.89823333774458
1101	5	8.98217769141354
1101	6	-7.331853146198535
1101	7	-0.8190144953058107
1101	8	11.897956293511324
1101	9	5.685675987368256
1101	10-14	13.094018646184585
1101	15-19	15.886495185019912
1101	20-24	16.87466936083373
1101	25-29	5.8822804449565265
1101	30-34	8.496965333434215
1101	35-39	10.05442669289199
1101	40-44	10.407973605263468
1101	45-49	8.633237184940015
1101	50-54	18.068311425590196
1101	55-59	18.276233185078787
1101	60-64	21.343253946519088
1101	65-69	16.020559581639105
1101	70-74	7.611547562778398
1101	75-79	11.106243186441976
1101	80-84	8.00025621334154
1101	85-89	13.837481110090934
1101	90-94	8.579296129762973
1101	95-99	10.805582444936562
1101	100-104	7.068299819262663
1101	105-109	5.621787466150884
1101	110-114	6.411087733185447
1101	115-119	3.1476933271321705
1101	120-124	4.561065368654781
1101	125-129	8.493095197252465
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-154	0.0
1101	155-159	0.0
1101	160-164	0.0
1101	165-169	0.0
1101	170-174	0.0
1101	175-179	0.0
1101	180-184	0.0
1101	185-189	0.0
1101	190-194	0.0
1101	195-199	0.0
1101	200-204	0.0
1101	205-209	0.0
1101	210-214	0.0
1101	215-219	0.0
1101	220-224	0.0
1101	225-229	0.0
1101	230-234	0.0
1101	235-239	0.0
1101	240-244	0.0
1101	245-249	0.0
1101	250-254	0.0
1101	255-259	0.0
1101	260-264	0.0
1101	265-269	0.0
1101	270-274	0.0
1101	275-279	0.0
1101	280-284	0.0
1101	285-289	0.0
1101	290-294	0.0
1101	295-299	0.0
1101	300-301	0.0
1102	1	0.5549317517827639
1102	2	1.0674799917466515
1102	3	7.571900448382841
1102	4	-9.107532874564386
1102	5	2.532365470519757
1102	6	-7.331853146198535
1102	7	-0.8190144953058107
1102	8	-10.227396866339976
1102	9	5.905553438769182
1102	10-14	3.3010399905616037
1102	15-19	1.761155742578742
1102	20-24	5.8628874296544105
1102	25-29	4.8446204409311395
1102	30-34	7.00192740805414
1102	35-39	5.317069844644227
1102	40-44	-0.08681245435135665
1102	45-49	6.327976658292478
1102	50-54	10.767990855649721
1102	55-59	8.438520211159656
1102	60-64	2.7350695332715613
1102	65-69	-0.5743171799255204
1102	70-74	3.021268214726618
1102	75-79	10.36982799231708
1102	80-84	6.61458037996514
1102	85-89	13.230737568748818
1102	90-94	7.881455404996149
1102	95-99	9.106197556176987
1102	100-104	5.80843943407557
1102	105-109	4.927391186410709
1102	110-114	5.697030864413492
1102	115-119	1.9258234005394783
1102	120-124	4.113927266289895
1102	125-129	8.04613912180767
1102	130-134	0.0
1102	135-139	0.0
1102	140-144	0.0
1102	145-149	0.0
1102	150-154	0.0
1102	155-159	0.0
1102	160-164	0.0
1102	165-169	0.0
1102	170-174	0.0
1102	175-179	0.0
1102	180-184	0.0
1102	185-189	0.0
1102	190-194	0.0
1102	195-199	0.0
1102	200-204	0.0
1102	205-209	0.0
1102	210-214	0.0
1102	215-219	0.0
1102	220-224	0.0
1102	225-229	0.0
1102	230-234	0.0
1102	235-239	0.0
1102	240-244	0.0
1102	245-249	0.0
1102	250-254	0.0
1102	255-259	0.0
1102	260-264	0.0
1102	265-269	0.0
1102	270-274	0.0
1102	275-279	0.0
1102	280-284	0.0
1102	285-289	0.0
1102	290-294	0.0
1102	295-299	0.0
1102	300-301	0.0
1103	1	0.9136774001916486
1103	2	2.7132203616795856
1103	3	-15.400440153557295
1103	4	-9.107532874564386
1103	5	3.317577949543743
1103	6	12.42435542212045
1103	7	-0.8190144953058107
1103	8	-10.227396866339976
1103	9	6.261825727895545
1103	10-14	-4.692679546352295
1103	15-19	-3.4758677869421746
1103	20-24	-3.330241592339071
1103	25-29	0.938640253002994
1103	30-34	4.97744490388909
1103	35-39	-1.1911208833581242
1103	40-44	-4.255850433653942
1103	45-49	7.021526018583593
1103	50-54	2.051032547503935
1103	55-59	-1.3110793087833557
1103	60-64	1.4340034002268673
1103	65-69	-0.6918097600847517
1103	70-74	2.9908462592907767
1103	75-79	-0.8377944862961613
1103	80-84	2.3885520383485392
1103	85-89	0.7124171207986674
1103	90-94	3.2282902363172674
1103	95-99	8.330927695366626
1103	100-104	5.670305946268748
1103	105-109	5.067024806534434
1103	110-114	5.982328662674863
1103	115-119	1.7409157946847493
1103	120-124	4.521549461721424
1103	125-129	8.417510999469851
1103	130-134	0.0
1103	135-139	0.0
1103	140-144	0.0
1103	145-149	0.0
1103	150-154	0.0
1103	155-159	0.0
1103	160-164	0.0
1103	165-169	0.0
1103	170-174	0.0
1103	175-179	0.0
1103	180-184	0.0
1103	185-189	0.0
1103	190-194	0.0
1103	195-199	0.0
1103	200-204	0.0
1103	205-209	0.0
1103	210-214	0.0
1103	215-219	0.0
1103	220-224	0.0
1103	225-229	0.0
1103	230-234	0.0
1103	235-239	0.0
1103	240-244	0.0
1103	245-249	0.0
1103	250-254	0.0
1103	255-259	0.0
1103	260-264	0.0
1103	265-269	0.0
1103	270-274	0.0
1103	275-279	0.0
1103	280-284	0.0
1103	285-289	0.0
1103	290-294	0.0
1103	295-299	0.0
1103	300-301	0.0
1117	1	0.1507599031245519
1117	2	0.048021467471222934
1117	3	8.633759572844895
1117	4	-9.107532874564386
1117	5	2.700359727030012
1117	6	16.176778213322017
1117	7	-0.8190144953058107
1117	8	-10.227396866339976
1117	9	6.136873212288599
1117	10-14	4.314466359854071
1117	15-19	2.751496522819087
1117	20-24	6.321966481485973
1117	25-29	11.50510244582396
1117	30-34	7.2121440734296804
1117	35-39	8.654467781480964
1117	40-44	8.837718310430631
1117	45-49	7.947784785946354
1117	50-54	2.5521561189962156
1117	55-59	2.830230667049012
1117	60-64	11.059640364559979
1117	65-69	12.739371362505448
1117	70-74	6.1572547685620655
1117	75-79	9.842325627741019
1117	80-84	5.210487810248193
1117	85-89	5.76130520802497
1117	90-94	5.74368192883059
1117	95-99	7.744360598634859
1117	100-104	5.360031301423547
1117	105-109	4.7976276515781
1117	110-114	4.941055423716893
1117	115-119	1.3409724107030065
1117	120-124	2.9542179474235333
1117	125-129	8.103408465858376
1117	130-134	0.0
1117	135-139	0.0
1117	140-144	0.0
1117	145-149	0.0
1117	150-154	0.0
1117	155-159	0.0
1117	160-164	0.0
1117	165-169	0.0
1117	170-174	0.0
1117	175-179	0.0
1117	180-184	0.0
1117	185-189	0.0
1117	190-194	0.0
1117	195-199	0.0
1117	200-204	0.0
1117	205-209	0.0
1117	210-214	0.0
1117	215-219	0.0
1117	220-224	0.0
1117	225-229	0.0
1117	230-234	0.0
1117	235-239	0.0
1117	240-244	0.0
1117	245-249	0.0
1117	250-254	0.0
1117	255-259	0.0
1117	260-264	0.0
1117	265-269	0.0
1117	270-274	0.0
1117	275-279	0.0
1117	280-284	0.0
1117	285-289	0.0
1117	290-294	0.0
1117	295-299	0.0
1117	300-301	0.0
2117	1	0.1468843587148605
2117	2	2.409685770153615
2117	3	-15.400440153557295
2117	4	-9.107532874564386
2117	5	2.9685595024308356
2117	6	-7.331853146198535
2117	7	-0.8190144953058107
2117	8	-10.227396866339976
2117	9	5.965934564918669
2117	10-14	-0.7046111952927347
2117	15-19	-2.9918951205889037
2117	20-24	5.376853550422368
2117	25-29	0.9924301964801412
2117	30-34	4.327757977178447
2117	35-39	3.710826514031524
2117	40-44	-2.609045887451181
2117	45-49	6.128112912783742
2117	50-54	2.2877698321026294
2117	55-59	2.574526997471567
2117	60-64	1.3217087886396346
2117	65-69	4.488980585966441
2117	70-74	2.2544768416932897
2117	75-79	9.495081361217252
2117	80-84	5.9943840302661044
2117	85-89	3.612086459378361
2117	90-94	5.572581005157243
2117	95-99	7.798513741496862
2117	100-104	5.578631004472527
2117	105-109	3.8966872429823383
2117	110-114	5.065147450837205
2117	115-119	0.8811888713289555
2117	120-124	3.4445712471283514
2117	125-129	7.115667274138097
2117	130-134	0.0
2117	135-139	0.0
2117	140-144	0.0
2117	145-149	0.0
2117	150-154	0.0
2117	155-159	0.0
2117	160-164	0.0
2117	165-169	0.0
2117	170-174	0.0
2117	175-179	0.0
2117	180-184	0.0
2117	185-189	0.0
2117	190-194	0.0
2117	195-199	0.0
2117	200-204	0.0
2117	205-209	0.0
2117	210-214	0.0
2117	215-219	0.0
2117	220-224	0.0
2117	225-229	0.0
2117	230-234	0.0
2117	235-239	0.0
2117	240-244	0.0
2117	245-249	0.0
2117	250-254	0.0
2117	255-259	0.0
2117	260-264	0.0
2117	265-269	0.0
2117	270-274	0.0
2117	275-279	0.0
2117	280-284	0.0
2117	285-289	0.0
2117	290-294	0.0
2117	295-299	0.0
2117	300-301	0.0
1116	1	0.5667772191418692
1116	2	3.03428785669076
1116	3	-15.400440153557295
1116	4	-9.107532874564386
1116	5	-15.563613304042882
1116	6	14.805094905749518
1116	7	-0.8190144953058107
1116	8	-10.227396866339976
1116	9	6.711979268453135
1116	10-14	-4.751497723030669
1116	15-19	-4.071569402705457
1116	20-24	0.5919424247173861
1116	25-29	-7.333689815266522
1116	30-34	-1.400710231294319
1116	35-39	-2.515681660959862
1116	40-44	-4.364426865187184
1116	45-49	-5.346124882360716
1116	50-54	-1.6919653329672322
1116	55-59	-4.897490032224354
1116	60-64	-2.5946049585163973
1116	65-69	4.400693830393237
1116	70-74	2.08116612760886
1116	75-79	2.556631479891603
1116	80-84	3.6973425265533173
1116	85-89	0.46396151557970455
1116	90-94	2.586613889141141
1116	95-99	7.35931946865221
1116	100-104	4.532649625074363
1116	105-109	4.435702107787645
1116	110-114	4.703890524776348
1116	115-119	0.6077234973956109
1116	120-124	2.6689600711229
1116	125-129	7.53349968289487
1116	130-134	0.0
1116	135-139	0.0
1116	140-144	0.0
1116	145-149	0.0
1116	150-154	0.0
1116	155-159	0.0
1116	160-164	0.0
1116	165-169	0.0
1116	170-174	0.0
1116	175-179	0.0
1116	180-184	0.0
1116	185-189	0.0
1116	190-194	0.0
1116	195-199	0.0
1116	200-204	0.0
1116	205-209	0.0
1116	210-214	0.0
1116	215-219	0.0
1116	220-224	0.0
1116	225-229	0.0
1116	230-234	0.0
1116	235-239	0.0
1116	240-244	0.0
1116	245-249	0.0
1116	250-254	0.0
1116	255-259	0.0
1116	260-264	0.0
1116	265-269	0.0
1116	270-274	0.0
1116	275-279	0.0
1116	280-284	0.0
1116	285-289	0.0
1116	290-294	0.0
1116	295-299	0.0
1116	300-301	0.0
2118	1	0.45416138556677055
2118	2	2.1557720836989667
2118	3	-15.400440153557295
2118	4	-9.107532874564386
2118	5	-15.563613304042882
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1107	125-129	-16.2383314859363
1107	130-134	0.0
1107	135-139	0.0
1107	140-144	0.0
1107	145-149	0.0
1107	150-154	0.0
1107	155-159	0.0
1107	160-164	0.0
1107	165-169	0.0
1107	170-174	0.0
1107	175-179	0.0
1107	180-184	0.0
1107	185-189	0.0
1107	190-194	0.0
1107	195-199	0.0
1107	200-204	0.0
1107	205-209	0.0
1107	210-214	0.0
1107	215-219	0.0
1107	220-224	0.0
1107	225-229	0.0
1107	230-234	0.0
1107	235-239	0.0
1107	240-244	0.0
1107	245-249	0.0
1107	250-254	0.0
1107	255-259	0.0
1107	260-264	0.0
1107	265-269	0.0
1107	270-274	0.0
1107	275-279	0.0
1107	280-284	0.0
1107	285-289	0.0
1107	290-294	0.0
1107	295-299	0.0
1107	300-301	0.0
1106	1	-5.492725091522935
1106	2	-23.023358247205344
1106	3	-15.400440153557295
1106	4	-9.107532874564386
1106	5	-15.563613304042882
1106	6	-7.331853146198535
1106	7	-0.8190144953058107
1106	8	-10.227396866339976
1106	9	-15.467549952326085
1106	10-14	-9.500448323759112
1106	15-19	-9.194103390236858
1106	20-24	-7.925651801062816
1106	25-29	-7.333689815266522
1106	30-34	-17.490025166359256
1106	35-39	-10.6496426999209
1106	40-44	-15.54260218986251
1106	45-49	-14.725252289742633
1106	50-54	-9.003361436863337
1106	55-59	-9.061580941315263
1106	60-64	-2.814701038989475
1106	65-69	-11.32560811765871
1106	70-74	-11.459524638501687
1106	75-79	-13.089578842779996
1106	80-84	-12.046117112854201
1106	85-89	-7.580385887017698
1106	90-94	-10.990075872610054
1106	95-99	-16.705300661217922
1106	100-104	-11.4536282766333
1106	105-109	-8.164728500944591
1106	110-114	-12.121728508041421
1106	115-119	-1.1202840309978654
1106	120-124	-11.065007366708947
1106	125-129	-16.2383314859363
1106	130-134	0.0
1106	135-139	0.0
1106	140-144	0.0
1106	145-149	0.0
1106	150-154	0.0
1106	155-159	0.0
1106	160-164	0.0
1106	165-169	0.0
1106	170-174	0.0
1106	175-179	0.0
1106	180-184	0.0
1106	185-189	0.0
1106	190-194	0.0
1106	195-199	0.0
1106	200-204	0.0
1106	205-209	0.0
1106	210-214	0.0
1106	215-219	0.0
1106	220-224	0.0
1106	225-229	0.0
1106	230-234	0.0
1106	235-239	0.0
1106	240-244	0.0
1106	245-249	0.0
1106	250-254	0.0
1106	255-259	0.0
1106	260-264	0.0
1106	265-269	0.0
1106	270-274	0.0
1106	275-279	0.0
1106	280-284	0.0
1106	285-289	0.0
1106	290-294	0.0
1106	295-299	0.0
1106	300-301	0.0
2101	1	-1.281789912205344
2101	2	-1.6377001984767574
2101	3	7.419178954858495
2101	4	14.817757049895663
2101	5	5.264474790435255
2101	6	16.8544645471731
2101	7	22.932405868562686
2101	8	-10.227396866339976
2101	9	5.828587934683171
2101	10-14	-8.741926377363885
2101	15-19	-4.475911394392883
2101	20-24	-12.120288094763133
2101	25-29	-0.1367241806245385
2101	30-34	-9.923760670136254
2101	35-39	-6.895732709496387
2101	40-44	-8.7637193299838
2101	45-49	-3.1669770433394326
2101	50-54	-5.547661894358815
2101	55-59	-9.061580941315263
2101	60-64	-6.604195867607306
2101	65-69	-8.29647735906737
2101	70-74	-11.86680671282871
2101	75-79	-13.434992568950285
2101	80-84	-8.937938054207553
2101	85-89	-7.580385887017698
2101	90-94	-7.631822537848189
2101	95-99	-16.705300661217922
2101	100-104	-8.482596074247066
2101	105-109	-8.893761218996218
2101	110-114	-8.889722299467183
2101	115-119	-0.5320099237475588
2101	120-124	-6.951193183989895
2101	125-129	-16.2383314859363
2101	130-134	0.0
2101	135-139	0.0
2101	140-144	0.0
2101	145-149	0.0
2101	150-154	0.0
2101	155-159	0.0
2101	160-164	0.0
2101	165-169	0.0
2101	170-174	0.0
2101	175-179	0.0
2101	180-184	0.0
2101	185-189	0.0
2101	190-194	0.0
2101	195-199	0.0
2101	200-204	0.0
2101	205-209	0.0
2101	210-214	0.0
2101	215-219	0.0
2101	220-224	0.0
2101	225-229	0.0
2101	230-234	0.0
2101	235-239	0.0
2101	240-244	0.0
2101	245-249	0.0
2101	250-254	0.0
2101	255-259	0.0
2101	260-264	0.0
2101	265-269	0.0
2101	270-274	0.0
2101	275-279	0.0
2101	280-284	0.0
2101	285-289	0.0
2101	290-294	0.0
2101	295-299	0.0
2101	300-301	0.0
2106	1	-0.49995866420855606
2106	2	-0.14115116578012277
2106	3	6.864945750608907
2106	4	14.582671526195137
2106	5	7.163204522384014
2106	6	19.019582126894285
2106	7	-0.8190144953058107
2106	8	10.648711700620819
2106	9	-15.467549952326085
2106	10-14	-4.9799322447247665
2106	15-19	-4.480116961675025
2106	20-24	-8.563357456988086
2106	25-29	-3.6693091607373143
2106	30-34	-17.490025166359256
2106	35-39	-6.8797644468213806
2106	40-44	-11.956724830318223
2106	45-49	-14.894383246748479
2106	50-54	-5.346692163993907
2106	55-59	-9.061580941315263
2106	60-64	-6.604195867607306
2106	65-69	-11.32560811765871
2106	70-74	-11.919813818168585
2106	75-79	-13.353016596426135
2106	80-84	-8.958415906374192
2106	85-89	-7.580385887017698
2106	90-94	-14.107481297337742
2106	95-99	-16.705300661217922
2106	100-104	-11.962541760748573
2106	105-109	-8.605883315584915
2106	110-114	-8.446086770797509
2106	115-119	-5.524924987622105
2106	120-124	-7.36735608750346
2106	125-129	-13.35028502106419
2106	130-134	0.0
2106	135-139	0.0
2106	140-144	0.0
2106	145-149	0.0
2106	150-154	0.0
2106	155-159	0.0
2106	160-164	0.0
2106	165-169	0.0
2106	170-174	0.0
2106	175-179	0.0
2106	180-184	0.0
2106	185-189	0.0
2106	190-194	0.0
2106	195-199	0.0
2106	200-204	0.0
2106	205-209	0.0
2106	210-214	0.0
2106	215-219	0.0
2106	220-224	0.0
2106	225-229	0.0
2106	230-234	0.0
2106	235-239	0.0
2106	240-244	0.0
2106	245-249	0.0
2106	250-254	0.0
2106	255-259	0.0
2106	260-264	0.0
2106	265-269	0.0
2106	270-274	0.0
2106	275-279	0.0
2106	280-284	0.0
2106	285-289	0.0
2106	290-294	0.0
2106	295-299	0.0
2106	300-301	0.0
2111	1	0.8988464090454187
2111	2	0.7651786216337086
2111	3	8.213566992067086
2111	4	-9.107532874564386
2111	5	1.6690069017166635
2111	6	13.036275647224047
2111	7	-0.8190144953058107
2111	8	10.933594911887393
2111	9	-15.467549952326085
2111	10-14	-1.014409804234301
2111	15-19	6.000897574497531
2111	20-24	-12.120288094763133
2111	25-29	0.31346700105649283
2111	30-34	-13.643006097012263
2111	35-39	-6.69175883784006
2111	40-44	-8.156419933394858
2111	45-49	-10.578416014004674
2111	50-54	-5.166311804906038
2111	55-59	-9.061580941315263
2111	60-64	-3.1187152597987744
2111	65-69	-7.826051490737785
2111	70-74	-7.050221305327538
2111	75-79	-13.107329899244089
2111	80-84	-11.85656682640486
2111	85-89	-7.580385887017698
2111	90-94	-7.20773705160809
2111	95-99	-16.705300661217922
2111	100-104	-11.956749397419653
2111	105-109	-8.099104275113165
2111	110-114	-8.255524020387499
2111	115-119	0.48408464226641
2111	120-124	-6.078208511972793
2111	125-129	-16.2383314859363
2111	130-134	0.0
2111	135-139	0.0
2111	140-144	0.0
2111	145-149	0.0
2111	150-154	0.0
2111	155-159	0.0
2111	160-164	0.0
2111	165-169	0.0
2111	170-174	0.0
2111	175-179	0.0
2111	180-184	0.0
2111	185-189	0.0
2111	190-194	0.0
2111	195-199	0.0
2111	200-204	0.0
2111	205-209	0.0
2111	210-214	0.0
2111	215-219	0.0
2111	220-224	0.0
2111	225-229	0.0
2111	230-234	0.0
2111	235-239	0.0
2111	240-244	0.0
2111	245-249	0.0
2111	250-254	0.0
2111	255-259	0.0
2111	260-264	0.0
2111	265-269	0.0
2111	270-274	0.0
2111	275-279	0.0
2111	280-284	0.0
2111	285-289	0.0
2111	290-294	0.0
2111	295-299	0.0
2111	300-301	0.0
2110	1	0.27064771014399014
2110	2	-0.20013235718181122
2110	3	9.220277046727684
2110	4	15.781648670805764
2110	5	7.052051973704909
2110	6	-7.331853146198535
2110	7	-0.8190144953058107
2110	8	10.915179614153988
2110	9	-15.467549952326085
2110	10-14	-8.920049193171529
2110	15-19	0.678258975694142
2110	20-24	-3.5823533379678665
2110	25-29	0.5961964085293285
2110	30-34	-4.031668984230972
2110	35-39	-6.722131311505779
2110	40-44	-8.108046176131644
2110	45-49	-6.422875627686118
2110	50-54	-1.2638161098916818
2110	55-59	-9.061580941315263
2110	60-64	-6.604195867607306
2110	65-69	-7.972025354645692
2110	70-74	-2.829496341552627
2110	75-79	-13.166819694670572
2110	80-84	-8.354794625587273
2110	85-89	-7.580385887017698
2110	90-94	-6.9365371322343385
2110	95-99	-16.705300661217922
2110	100-104	-8.384807308463614
2110	105-109	-7.80653333211626
2110	110-114	-12.068261021123726
2110	115-119	-1.0691017152110334
2110	120-124	-6.503628907187588
2110	125-129	-16.2383314859363
2110	130-134	0.0
2110	135-139	0.0
2110	140-144	0.0
2110	145-149	0.0
2110	150-154	0.0
2110	155-159	0.0
2110	160-164	0.0
2110	165-169	0.0
2110	170-174	0.0
2110	175-179	0.0
2110	180-184	0.0
2110	185-189	0.0
2110	190-194	0.0
2110	195-199	0.0
2110	200-204	0.0
2110	205-209	0.0
2110	210-214	0.0
2110	215-219	0.0
2110	220-224	0.0
2110	225-229	0.0
2110	230-234	0.0
2110	235-239	0.0
2110	240-244	0.0
2110	245-249	0.0
2110	250-254	0.0
2110	255-259	0.0
2110	260-264	0.0
2110	265-269	0.0
2110	270-274	0.0
2110	275-279	0.0
2110	280-284	0.0
2110	285-289	0.0
2110	290-294	0.0
2110	295-299	0.0
2110	300-301	0.0
2109	1	0.7808879863152178
2109	2	0.8021019857116123
2109	3	7.023629341084771
2109	4	15.427119335187692
2109	5	8.430827970025558
2109	6	17.334242852786943
2109	7	-0.8190144953058107
2109	8	10.182617294672852
2109	9	5.663978380267494
2109	10-14	12.14392990881374
2109	15-19	3.9276432310507854
2109	20-24	-3.5420072839923087
2109	25-29	0.48866767633198194
2109	30-34	0.9438682667352403
2109	35-39	-2.7257528599192096
2109	40-44	-3.7648920467728715
2109	45-49	-9.968874786769076
2109	50-54	-1.368998788119864
2109	55-59	-9.061580941315263
2109	60-64	-6.604195867607306
2109	65-69	-4.444032229425032
2109	70-74	2.131351550412127
2109	75-79	-9.191258551293366
2109	80-84	-8.460881962487749
2109	85-89	-7.580385887017698
2109	90-94	-7.107357066852115
2109	95-99	-16.705300661217922
2109	100-104	-7.9680736496166205
2109	105-109	-7.844858428620759
2109	110-114	-3.818056689423866
2109	115-119	1.0188109157103966
2109	120-124	-6.281843367966488
2109	125-129	-13.268526147023104
2109	130-134	0.0
2109	135-139	0.0
2109	140-144	0.0
2109	145-149	0.0
2109	150-154	0.0
2109	155-159	0.0
2109	160-164	0.0
2109	165-169	0.0
2109	170-174	0.0
2109	175-179	0.0
2109	180-184	0.0
2109	185-189	0.0
2109	190-194	0.0
2109	195-199	0.0
2109	200-204	0.0
2109	205-209	0.0
2109	210-214	0.0
2109	215-219	0.0
2109	220-224	0.0
2109	225-229	0.0
2109	230-234	0.0
2109	235-239	0.0
2109	240-244	0.0
2109	245-249	0.0
2109	250-254	0.0
2109	255-259	0.0
2109	260-264	0.0
2109	265-269	0.0
2109	270-274	0.0
2109	275-279	0.0
2109	280-284	0.0
2109	285-289	0.0
2109	290-294	0.0
2109	295-299	0.0
2109	300-301	0.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	1083.0
3	937353.0
4	1722025.0
5	1046386.0
6	921567.0
7	1527997.0
8	2262620.0
9	2391394.0
10	2193572.0
11	1401950.0
12	919754.0
13	482811.0
14	157972.0
15	68991.0
16	13807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.5631591422113456	12.29277871115368	71.30462897124032	9.839433175394666
2	82.32061779084898	7.687119035126987	5.718685670185302	4.273577503838729
3	8.099014650681049	79.5272449361118	4.640022662909159	7.733717750297994
4	5.918592241164944	78.81713732368556	5.483481322251788	9.78078911289771
5	17.269407593057696	72.53790956494412	3.8197187650539277	6.372964076944259
6	5.393528743159195	82.64739960251	5.192383658431645	6.766687995899151
7	8.143876471118876	9.29326888169663	12.012734845467888	70.5501198017166
8	9.638040544149574	78.68756656973021	4.179367293547503	7.495025592572723
9	7.578092733850338	7.951211460656259	80.00119509708175	4.469500708411657
10-14	29.807894129465613	8.469303597124169	40.232048254049346	21.490754019360878
15-19	34.57135229379262	28.3737152621708	12.153238788755361	24.901693655281214
20-24	20.055252865078355	4.697801237159733	62.60784916489597	12.639096732865942
25-29	30.942832853349067	23.85096916881183	13.732891295746185	31.47330668209291
30-34	28.704223950304698	18.86311912082168	44.23261739669584	8.200039532177783
35-39	37.83609967480096	13.121800338039517	28.931032843617448	20.11106714354207
40-44	12.786739505881014	24.582782279018247	42.86568733541622	19.764790879684515
45-49	13.596245430605848	44.51590310600714	28.70491407064074	13.182937392746268
50-54	30.672398012622416	32.51598340462928	17.88123685790482	18.930381724843485
55-59	4.388470048687379	25.669813529562273	64.33946375357971	5.602252668170638
60-64	3.589234636701738	18.322976581047214	40.79853405919868	37.289254723052366
65-69	22.540337172177473	23.075866997632026	26.442007175387	27.941788654803503
70-74	16.287978305284316	32.11957833869042	32.025826328621115	19.566617027404146
75-79	9.738088858019866	44.32355523666533	27.56451372512943	18.373842180185378
80-84	25.299379293734788	33.3835711600464	32.10530208253273	9.21174746368609
85-89	3.7250216791424364	13.197129673722063	6.165371391664106	76.91247725547139
90-94	22.48607339584076	33.016415317656964	25.161965400873115	19.33554588562916
95-99	3.8616909061895024	13.969689030618415	41.975520706913194	40.19309935627889
100-104	4.113800607598823	40.65416561884825	40.52658100532957	14.70545276822336
105-109	4.008036661617233	19.84147326599639	54.74039138614345	21.41009868624292
110-114	4.949093790919447	34.57040537598552	28.02217082653114	32.458330006563884
115-119	17.24690556569172	27.004951741864303	26.355612312146693	29.392530380297288
120-124	8.077982318484604	51.92409790623606	14.901029178351836	25.09689059692751
125-129	3.5954046419615153	48.51761043981857	10.448797267031916	37.438187651187995
130-134	NaN	NaN	NaN	NaN
135-139	NaN	NaN	NaN	NaN
140-144	NaN	NaN	NaN	NaN
145-149	NaN	NaN	NaN	NaN
150-154	NaN	NaN	NaN	NaN
155-159	NaN	NaN	NaN	NaN
160-164	NaN	NaN	NaN	NaN
165-169	NaN	NaN	NaN	NaN
170-174	NaN	NaN	NaN	NaN
175-179	NaN	NaN	NaN	NaN
180-184	NaN	NaN	NaN	NaN
185-189	NaN	NaN	NaN	NaN
190-194	NaN	NaN	NaN	NaN
195-199	NaN	NaN	NaN	NaN
200-204	NaN	NaN	NaN	NaN
205-209	NaN	NaN	NaN	NaN
210-214	NaN	NaN	NaN	NaN
215-219	NaN	NaN	NaN	NaN
220-224	NaN	NaN	NaN	NaN
225-229	NaN	NaN	NaN	NaN
230-234	NaN	NaN	NaN	NaN
235-239	NaN	NaN	NaN	NaN
240-244	NaN	NaN	NaN	NaN
245-249	NaN	NaN	NaN	NaN
250-254	NaN	NaN	NaN	NaN
255-259	NaN	NaN	NaN	NaN
260-264	NaN	NaN	NaN	NaN
265-269	NaN	NaN	NaN	NaN
270-274	NaN	NaN	NaN	NaN
275-279	NaN	NaN	NaN	NaN
280-284	NaN	NaN	NaN	NaN
285-289	NaN	NaN	NaN	NaN
290-294	NaN	NaN	NaN	NaN
295-299	NaN	NaN	NaN	NaN
300-301	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1888.0
1	24200.75
2	90049.0
3	171463.0
4	255283.0
5	278532.0
6	241166.75
7	225786.5
8	236407.0
9	272789.0
10	344174.25
11	429129.25
12	481567.5
13	492343.75
14	457679.75
15	397799.5
16	314753.0
17	229111.0
18	162354.0
19	109854.0
20	65672.25
21	35052.0
22	17046.5
23	7943.25
24	3889.0
25	2090.25
26	1320.5
27	881.25
28	550.5
29	365.75
30	216.0
31	195.5
32	122.25
33	97.5
34	92.5
35	104.25
36	82.75
37	41.25
38	25.5
39	12.5
40	4.75
41	12.75
42	6.75
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	8.723131664083166E-5
2	2.915750374378119
3	35.29149154460617
4	64.46910210687307
5	24.57980986314528
6	71.70279642416402
7	97.16809138253039
8	54.622412392030995
9	23.255189858337587
10-14	38.49183284336333
15-19	47.34292661815027
20-24	43.30021617166425
25-29	63.557663202628
30-34	20.5438386589506
35-39	52.26136720633359
40-44	24.827788557768503
45-49	21.80389627398908
50-54	53.52292520001829
55-59	54.00880986451606
60-64	68.05548933590924
65-69	41.28194519854533
70-74	12.217602008613222
75-79	29.791459829791762
80-84	14.099431987050885
85-89	60.15775035917494
90-94	15.972295832299539
95-99	28.06222234739224
100-104	14.08359077994891
105-109	12.404341826631248
110-114	14.563148681666881
115-119	2.9221668607978852
120-124	11.603965834733291
125-129	26.760039483386233
130-134	100.0
135-139	100.0
140-144	100.0
145-149	100.0
150-154	100.0
155-159	100.0
160-164	100.0
165-169	100.0
170-174	100.0
175-179	100.0
180-184	100.0
185-189	100.0
190-194	100.0
195-199	100.0
200-204	100.0
205-209	100.0
210-214	100.0
215-219	100.0
220-224	100.0
225-229	100.0
230-234	100.0
235-239	100.0
240-244	100.0
245-249	100.0
250-254	100.0
255-259	100.0
260-264	100.0
265-269	100.0
270-274	100.0
275-279	100.0
280-284	100.0
285-289	100.0
290-294	100.0
295-299	100.0
300-301	100.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	1.6049282E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.1550887278028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.39789425877417	84.9796492155559
2	2.148853820371974	3.788647983959387
3	0.56378437067809	1.4910138366142645
4	0.21852480444685454	0.7705629410095287
5	0.1776869685915342	0.7832005240980504
6	0.0799334725400496	0.4227925418453677
7	0.08062670939234871	0.49753583042192906
8	0.05998212866503897	0.4230183899639195
9	0.043920210769659664	0.3484611069608833
>10	0.2077592888243472	3.2726666748198894
>50	0.012544767478317115	0.7721626418993035
>100	0.007521358649393065	1.2113123713131455
>500	5.59567943013049E-4	0.3252787949989604
>1k	3.778136829992368E-4	0.6263196045295287
>5k	2.2932327999233785E-5	0.15952831525089284
>10k+	7.5268642113169154E-6	0.1278492267590528
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGAAANNATCTTTGGCAAATGCTTNCGNATTAGTTAGNCCTTAATAAATC	19268	0.12005521493111031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	2.49232333259519E-6	0.0	0.0
15-19	6.230808331487975E-6	0.0	0.0
20-24	6.230808331487975E-6	0.0	0.0
25-29	6.230808331487975E-6	0.0	0.0
30-34	6.230808331487975E-6	0.0	0.0
35-39	7.476969997785571E-6	0.0	0.0
40-44	1.8692424994463924E-5	0.0	0.0
45-49	3.240020332373747E-5	0.0	0.0
50-54	5.856959831598697E-5	0.0	0.0
55-59	9.844677163751E-5	0.0	0.0
60-64	1.7072414828277053E-4	0.0	0.0
65-69	4.511105231997294E-4	0.0	6.230808331487975E-6
70-74	0.0011551918646578706	4.98464666519038E-6	6.230808331487975E-6
75-79	0.0019165966427657014	6.230808331487975E-6	6.230808331487975E-6
80-84	0.0023353069626416935	6.230808331487975E-6	6.230808331487975E-6
85-89	0.0028910950658104207	6.230808331487975E-6	6.230808331487975E-6
90-94	0.00539961850006748	6.230808331487975E-6	8.847747830712926E-5
95-99	0.009191688450611062	6.230808331487975E-6	1.4330859162422344E-4
100-104	0.013500915492668148	6.230808331487975E-6	1.4330859162422344E-4
105-109	0.018894303184404136	6.230808331487975E-6	2.0187818994021038E-4
110-114	0.025079003534239103	6.230808331487975E-6	2.1807829160207915E-4
115-119	0.03177961481392126	6.230808331487975E-6	2.1807829160207915E-4
120-124	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
125-129	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
130-134	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
135-139	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
140-144	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
145-149	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
150-154	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
155-159	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
160-164	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
165-169	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
170-174	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
175-179	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
180-184	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
185-189	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
190-194	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
195-199	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
200-204	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
205-209	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
210-214	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
215-219	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
220-224	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
225-229	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
230-234	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
235-239	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
240-244	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
245-249	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
250-254	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
255-259	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
260-264	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
265-269	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
270-274	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
275-279	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
280-284	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
285-289	0.03338467104011257	6.230808331487975E-6	2.1807829160207915E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAAAAC	14600	0.0	1107.1533	1
TGAAGAC	2950	0.0	748.5249	1
TGAACAC	5965	0.0	591.85126	1
CGAAGAC	2850	0.0	591.53046	1
TCCTTGG	33950	0.0	551.40796	9
ATTCTTG	17980	0.0	544.0632	8
ATCCTTG	32350	0.0	534.9281	8
TCTTTGG	12125	0.0	514.26447	9
TCGTTGG	2250	0.0	510.17932	9
TGATGGG	325	0.0	508.55563	1
TTCTTGG	19855	0.0	504.4721	9
ATCTTTG	12080	0.0	504.41736	8
TGAAGAT	705	0.0	497.01395	1
GCTTTCG	40515	0.0	479.52032	20-24
TGCTTTC	37645	0.0	476.82394	20-24
TGAAAGC	345	0.0	459.91113	1
TGAAAAT	4175	0.0	457.63916	1
TCCATGG	845	0.0	441.8311	9
TAATTGG	255	0.0	437.04684	9
GTAATCG	625	0.0	427.95627	8
>>END_MODULE