##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AGE3A l01n02.3320000000daac.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16049282
Sequences flagged as poor quality	0
Sequence length	12
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.593666931642176	34.0	23.0	34.0	12.0	34.0
2	29.033684185996606	34.0	24.0	34.0	12.0	34.0
3	28.900404454230415	34.0	24.0	34.0	12.0	34.0
4	28.907883418086865	34.0	24.0	34.0	12.0	34.0
5	29.832523411327685	34.0	31.0	34.0	11.0	34.0
6	31.3666795810554	37.0	26.0	38.0	12.0	38.0
7	31.124978114285735	37.0	26.0	38.0	12.0	38.0
8	31.07098361160331	37.0	26.0	38.0	12.0	38.0
9	31.003891762883846	37.0	26.0	38.0	11.0	38.0
10	31.5345031634437	37.0	27.0	38.0	11.0	38.0
11	31.168860762743154	37.0	26.0	38.0	11.0	38.0
12	30.76155593751795	37.0	26.0	38.0	11.0	38.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.4313737418893808
1101	2	-0.08964510323891162
1101	3	0.007961632122523099
1101	4	-0.007343139548353861
1101	5	-0.05260660496884739
1101	6	0.07125869585527767
1101	7	0.05503701453362808
1101	8	0.06665951486965582
1101	9	0.1494014605665086
1101	10	0.07843925506666594
1101	11	0.021049949835543202
1101	12	0.16336312552821042
1102	1	-0.04117456676825526
1102	2	0.02980790996352667
1102	3	-0.013797767235299574
1102	4	-0.008446915990294457
1102	5	-0.0541073364323168
1102	6	-0.10832697153609061
1102	7	-0.11087544731826426
1102	8	-0.12909080171770881
1102	9	-0.08800040831007294
1102	10	-0.12984702997870912
1102	11	-0.06177746324475919
1102	12	-0.1109871497367827
1103	1	0.5786789618387225
1103	2	0.4618658632486685
1103	3	0.660367815330229
1103	4	0.5920248276634474
1103	5	0.39821406001250637
1103	6	0.921687509041174
1103	7	1.1000178460306635
1103	8	0.48462895193027933
1103	9	0.5965315478151041
1103	10	0.810345391010582
1103	11	0.8407957257948624
1103	12	0.5608107482329281
1117	1	-0.27163917793902215
1117	2	-0.14517062954983473
1117	3	-0.02674736608440753
1117	4	-0.13285339010256791
1117	5	-0.11682385199560841
1117	6	0.03075815974273155
1117	7	-0.25898772034231143
1117	8	-0.6659956439634236
1117	9	-0.16244735157873436
1117	10	-0.1764391519602455
1117	11	-0.2678099422380562
1117	12	-0.4312118166932848
2117	1	-0.07869938889157169
2117	2	-0.031012650421498478
2117	3	-0.042277732497751686
2117	4	-0.16837216431744295
2117	5	-0.0954892140640844
2117	6	-0.14207950617873877
2117	7	-0.5375829862219348
2117	8	-0.0631300043962888
2117	9	-0.028063288222405447
2117	10	-0.1516061966074176
2117	11	-0.39473473358846434
2117	12	-0.14489096829983694
1116	1	0.032860810216917
1116	2	-0.22590986181779726
1116	3	-0.09213314195924127
1116	4	-0.25682841986077065
1116	5	-0.30115447748047686
1116	6	-0.15341941826521577
1116	7	-0.6599421517977682
1116	8	-0.10627237304829151
1116	9	-0.17570378734543013
1116	10	-0.22860234387549028
1116	11	-0.07133640039002742
1116	12	-0.11512489460944053
2118	1	0.6297608363843565
2118	2	0.46849674887331005
2118	3	0.4979387758294962
2118	4	0.6264717633112902
2118	5	0.33545371776273925
2118	6	0.7807635603855694
2118	7	0.38827947928687223
2118	8	0.8208470827258942
2118	9	0.8020544312209346
2118	10	0.6508344091437159
2118	11	0.7636215539421052
2118	12	0.6622997673470898
1119	1	-0.15263076247896024
1119	2	-0.1226035808397512
1119	3	-0.11717214472581361
1119	4	-0.17498198405760235
1119	5	-0.18069649629575935
1119	6	-0.17635069194877317
1119	7	-0.8430044462579112
1119	8	-0.22383142363115027
1119	9	-0.024737094554559746
1119	10	-0.29873961281276706
1119	11	-0.22069738359921232
1119	12	-0.3854726898019578
2119	1	-0.1467414777569367
2119	2	-0.19293252974816966
2119	3	-0.06613797069795169
2119	4	-0.024968932264755495
2119	5	-0.12489656993957254
2119	6	-0.052393753307367064
2119	7	-0.6384662435733937
2119	8	-0.17675520422900348
2119	9	-0.0018390304242252853
2119	10	-0.1873558572185594
2119	11	-0.11170384383872545
2119	12	-0.1631301889125112
1118	1	-0.0993243566472728
1118	2	-0.40426940567216363
1118	3	-0.2373403649714021
1118	4	-0.19896986314799037
1118	5	-0.16963726607142604
1118	6	-0.1580242182137752
1118	7	-0.17156019632216513
1118	8	0.02445144979815339
1118	9	0.10317131695839166
1118	10	-0.09996884996420263
1118	11	-0.27104812180128235
1118	12	0.016141779388330946
1113	1	-0.1105080650166812
1113	2	-0.19469071110050962
1113	3	-0.2422798518773881
1113	4	-0.27079861619613865
1113	5	-0.23856851831452985
1113	6	-0.2034919516108893
1113	7	-0.2346994945238734
1113	8	-0.1770288318498352
1113	9	-0.07628821344649594
1113	10	-0.09512802737896564
1113	11	-0.3196852795253058
1113	12	-0.15227627944211264
2113	1	0.5482190330616952
2113	2	0.5346856931600428
2113	3	0.29851129054282666
2113	4	0.46494478726274835
2113	5	0.24056080641399902
2113	6	0.7532435492821286
2113	7	0.7336761899491222
2113	8	0.8285358466923292
2113	9	0.8859703163618384
2113	10	0.7613656558322042
2113	11	0.636267399679042
2113	12	0.8433845494620762
1112	1	-0.1435502945604874
1112	2	-0.13745635579931914
1112	3	-0.1835034922589891
1112	4	-0.15678581214923426
1112	5	-0.17246214753525635
1112	6	-0.1392004412336476
1112	7	-0.17907322001886072
1112	8	0.09480420322139693
1112	9	-0.06950831711155203
1112	10	0.006565689433966071
1112	11	-0.33588935173008494
1112	12	-0.15714973700643142
2114	1	-0.19172720487080497
2114	2	-0.2669304169038611
2114	3	-0.05457289970174273
2114	4	-0.25534165993587266
2114	5	-0.09714106760953811
2114	6	-0.05419773795803806
2114	7	-0.07143793890305261
2114	8	0.17857125109172856
2114	9	0.007262856457220579
2114	10	0.094183127348483
2114	11	-0.24946198330660607
2114	12	0.062330622144664005
1115	1	-0.10683853616218641
1115	2	-0.14596821166063734
1115	3	-0.24413069193267134
1115	4	-0.1581035958979271
1115	5	-0.10919467552306372
1115	6	-0.23661208034414116
1115	7	-0.05736106816113562
1115	8	0.13745095369946725
1115	9	-0.0165273324225943
1115	10	-0.19196975625028045
1115	11	-0.113359433521655
1115	12	-0.02694960117210954
1114	1	-0.12650932532191916
1114	2	-0.12804465207240412
1114	3	-0.217072120928119
1114	4	-0.19255432065542166
1114	5	-0.16574760718327397
1114	6	-0.14066433107101872
1114	7	-0.2030832872801298
1114	8	0.07180884207733484
1114	9	-0.007553492436077391
1114	10	-0.25388072290386887
1114	11	-0.29165807628514173
1114	12	-0.09436360033603108
1109	1	-0.1675853718349991
1109	2	-0.0747770042366831
1109	3	-0.286131980578439
1109	4	-0.17316565124581018
1109	5	-0.15231916346940366
1109	6	-0.36441411535665225
1109	7	-0.20469349578561236
1109	8	-0.4149072210461888
1109	9	-0.12747821651580793
1109	10	-0.35470859561025847
1109	11	-0.40976669197695514
1109	12	-0.45464067551020193
1108	1	-0.012423380628945324
1108	2	-0.032190061060550335
1108	3	-0.09853201841318437
1108	4	-0.10630860560747735
1108	5	-0.0705101896831053
1108	6	-0.3827967076425054
1108	7	-0.12367168034557707
1108	8	-0.4645683446977884
1108	9	-0.23099629953221523
1108	10	-0.310726308383817
1108	11	-0.14916032939787627
1108	12	-0.17766966078306012
1111	1	-0.12104934074573137
1111	2	-0.12922528891625618
1111	3	-0.08282103071164926
1111	4	-0.12517657543438432
1111	5	-0.15771098284420404
1111	6	-0.1653968043459919
1111	7	-0.1756748945099531
1111	8	-0.7076955179128142
1111	9	-0.2572789417608732
1111	10	-0.2567770320594214
1111	11	-0.028840501365241522
1111	12	-0.19592982868070408
1110	1	-0.6367538155359611
1110	2	-0.03111787212272432
1110	3	0.3623418260171114
1110	4	0.46823394770681404
1110	5	0.3143358685600255
1110	6	0.6324902345079799
1110	7	0.7032915309385608
1110	8	0.7123120334759001
1110	9	0.6858329813806137
1110	10	0.5496402902294797
1110	11	0.6864275170229881
1110	12	0.7195361367514437
1105	1	0.09594635756717196
1105	2	0.03182134573751583
1105	3	0.09678709101808636
1105	4	0.2161270188011244
1105	5	0.05979348389857009
1105	6	-0.25003805700611537
1105	7	0.1720230687329085
1105	8	-0.2875564231111767
1105	9	0.027360349380760596
1105	10	-0.31911204419055395
1105	11	0.1832184196564306
1105	12	0.0976928951129068
1104	1	0.33762176342724004
1104	2	-0.007855470092518857
1104	3	0.020918106313526863
1104	4	0.0672635777755275
1104	5	0.17766266799806374
1104	6	-0.020619335180324327
1104	7	0.1754635812820169
1104	8	0.10957093306399912
1104	9	0.1053567224492582
1104	10	0.0469834323906646
1104	11	0.26872642978356254
1104	12	0.05565686425776306
1107	1	0.2607003664642349
1107	2	0.19028422266930178
1107	3	0.036031170976070115
1107	4	-0.13529249667116972
1107	5	0.20291474507446594
1107	6	-0.3014260255376122
1107	7	-0.07113091019924411
1107	8	0.09402844462450588
1107	9	-0.1799766990416778
1107	10	-0.17590518521102538
1107	11	-0.13798170978046542
1107	12	0.1193526397651361
1106	1	0.10786398231621064
1106	2	0.2437397502591594
1106	3	0.014757304332650989
1106	4	0.16830370098871583
1106	5	-0.03108986850623552
1106	6	-0.16877384416242336
1106	7	0.23302055980040492
1106	8	0.059343631705136346
1106	9	-0.27243529286871393
1106	10	-0.06656730896852281
1106	11	-0.05572557145914914
1106	12	0.020322733559467565
2101	1	0.06300049418440423
2101	2	0.3393315668779877
2101	3	0.1450541831637686
2101	4	0.25060687306207186
2101	5	0.1728461225419089
2101	6	0.4178021402592478
2101	7	0.50299770744396
2101	8	0.06037257432798171
2101	9	-0.32836131653822775
2101	10	0.1510252363589366
2101	11	0.32228860358248923
2101	12	0.03875112390869617
2106	1	0.1614178454412709
2106	2	-0.15422924181231323
2106	3	0.025932133265925472
2106	4	-0.3879932655701843
2106	5	-0.014360957279020425
2106	6	-0.21393768030028681
2106	7	0.17117767345126111
2106	8	-0.4330198193080541
2106	9	-0.7529543280478848
2106	10	0.08205798582608281
2106	11	-0.07839834255263511
2106	12	-0.07583421753826869
2111	1	0.132105126337283
2111	2	0.017536340832378983
2111	3	-0.22764133689804567
2111	4	0.02391653887383427
2111	5	0.1664398343352964
2111	6	-0.05398627166922765
2111	7	0.15173487500866756
2111	8	0.1656687419363294
2111	9	-0.19242466300027417
2111	10	0.02110704089195181
2111	11	0.07419502327863725
2111	12	-0.07503536815939071
2110	1	-0.05656038253924578
2110	2	0.17730935020789929
2110	3	-0.012486035627457426
2110	4	0.07759654122921944
2110	5	0.15556735422416423
2110	6	0.08629085181833673
2110	7	0.16182683805324416
2110	8	0.10385368704093167
2110	9	-0.04543247712323506
2110	10	0.035371028037467056
2110	11	-0.08446858990973993
2110	12	-0.07049493468723966
2109	1	-0.053086387651053
2109	2	0.019150255236329627
2109	3	0.07817661818740262
2109	4	-0.02120416802147318
2109	5	0.08072833437404014
2109	6	-0.20814475802384536
2109	7	-0.007301182950072871
2109	8	-0.16305653336940296
2109	9	-0.3249354323094309
2109	10	0.009415481803785042
2109	11	-0.14308687306426648
2109	12	-0.5284813740894201
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	167.0
11	8897.0
12	37946.0
13	92395.0
14	165258.0
15	246890.0
16	325929.0
17	393956.0
18	439582.0
19	459362.0
20	459944.0
21	446085.0
22	429343.0
23	407800.0
24	387090.0
25	366532.0
26	349012.0
27	339788.0
28	336675.0
29	343142.0
30	363176.0
31	399082.0
32	465249.0
33	582447.0
34	814276.0
35	1528665.0
36	5860593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.671275767775228	24.859892302829394	40.527286583475714	13.941545345919664
2	9.003562776647925	26.87409653267935	27.17247128843163	36.9498694022411
3	45.540675622789585	15.932751130720435	16.867808595288476	21.65876465120151
4	15.403693162329205	32.373531250403055	26.744437959557192	25.47833762771055
5	63.08563604811954	9.72400570467923	22.09138216222895	5.09897608497228
6	22.203443257053074	23.93452392041737	25.32928422151746	28.5327486010121
7	19.38036974989721	31.003974570574222	28.55374642639754	21.061909253131027
8	22.998619065863448	23.67092332672099	25.73258577751863	27.59787182989693
9	23.101140792537684	24.883719089970448	28.661651571805614	23.35348854568625
10	18.588284422211814	9.300186095564031	45.50894211398006	26.602587368244095
11	20.31578860137099	16.731285345430738	31.910688086276263	31.04223796692201
12	23.328348686046976	26.585622532599594	21.804847320253618	28.281181461099813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1170.0
1	1170.0
2	1170.0
3	1170.0
4	5419.0
5	9668.0
6	9668.0
7	9668.0
8	9668.0
9	9668.0
10	9668.0
11	9668.0
12	9668.0
13	29991.5
14	50315.0
15	50315.0
16	50315.0
17	50315.0
18	50315.0
19	50315.0
20	50315.0
21	121698.5
22	193082.0
23	193082.0
24	193082.0
25	193082.0
26	193082.0
27	193082.0
28	193082.0
29	409374.5
30	625667.0
31	625667.0
32	625667.0
33	625667.0
34	625667.0
35	625667.0
36	625667.0
37	625667.0
38	2450779.0
39	4275891.0
40	4275891.0
41	4275891.0
42	4275891.0
43	4275891.0
44	4275891.0
45	4275891.0
46	5212461.0
47	6149031.0
48	6149031.0
49	6149031.0
50	6149031.0
51	6149031.0
52	6149031.0
53	6149031.0
54	5229994.0
55	4310957.0
56	4310957.0
57	4310957.0
58	4310957.0
59	4310957.0
60	4310957.0
61	4310957.0
62	4310957.0
63	2334233.0
64	357509.0
65	357509.0
66	357509.0
67	357509.0
68	357509.0
69	357509.0
70	357509.0
71	210075.5
72	62642.0
73	62642.0
74	62642.0
75	62642.0
76	62642.0
77	62642.0
78	62642.0
79	35601.0
80	8560.0
81	8560.0
82	8560.0
83	8560.0
84	8560.0
85	8560.0
86	8560.0
87	8560.0
88	5224.0
89	1888.0
90	1888.0
91	1888.0
92	1888.0
93	1888.0
94	1888.0
95	1888.0
96	2395.0
97	2902.0
98	2902.0
99	2902.0
100	2902.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.3084697496124748E-4
2	1.2461616662975952E-5
3	6.230808331487976E-6
4	6.230808331487976E-6
5	6.230808331487976E-6
6	6.230808331487976E-6
7	6.230808331487976E-6
8	6.230808331487976E-6
9	6.230808331487976E-6
10	6.230808331487976E-6
11	6.230808331487976E-6
12	1.2461616662975952E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	1.6049282E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.159298966809633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.48429682436876	2.3493507742256563
2	13.110409220033754	1.0906022304667575
3	5.98986263626565	0.7474088842305404
4	4.011593370481026	0.6674166464440843
5	3.3169477755083667	0.689808872781673
6	2.4552048462500706	0.6127158588188354
7	1.7001561097768945	0.4950020285567085
8	1.3579524305645847	0.4518504113139126
9	1.0332428498209314	0.3867809325951277
>10	8.588819105777102	7.241735411932113
>50	1.0864235611247668	3.150332589590428
>100	0.7237872565082053	5.80411249320706
>500	0.07081249249954127	2.039677415808289
>1k	0.05990475462902562	5.091310586892332
>5k	0.0071532511169576886	1.9866595425589333
>10k+	0.0034335152745110535	67.19523532057752
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTTGGTCAATCT	796226	4.961131594547345	No Hit
TCTAGCGTAGTG	708789	4.416328406467031	No Hit
AAGAGATGTCGA	700360	4.363808923040918	No Hit
ACGTGTACCCAA	695190	4.331595643967126	No Hit
GACTTTCCCTCG	664709	4.141674375215041	No Hit
ACGCGCAGATAC	607580	3.7857145260454637	No Hit
CTGCTAACGCAA	603431	3.7598629022781207	No Hit
TCCAAAGTGTTC	586921	3.656992256725254	No Hit
TTAGGGCTCGTA	582353	3.628529924267017	No Hit
TCACGGGAGTTG	582301	3.62820592223378	No Hit
GGATGGTGTTGC	541764	3.3756276449002516	No Hit
TCGAGGACTGCA	539755	3.3631099509622926	No Hit
TACAGATGGCTC	538071	3.3526172697320664	No Hit
ATCCCGAATTTG	495820	3.089359386918368	No Hit
ATGTGCACGACT	491859	3.0646791551173442	No Hit
CGGAGCTATGGT	488584	3.044273257831721	No Hit
TAGCTCGTAACT	468843	2.921270870559817	No Hit
TAGGATTGCTCG	416165	2.5930443492736934	No Hit
GCGATATATCGC	383057	2.3867547470347894	No Hit
CAGTGCATATGC	360188	2.244262391301991	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTC	1400	0.0	6.000016	1
GTGGGCC	75	1.7513134E-4	6.000016	1
TTATGCA	75	1.7513134E-4	6.000016	1
TCTAGAG	605	0.0	6.000016	1
TTGAGGC	75	1.7513134E-4	6.000016	1
CTGCGAA	1100	0.0	6.000016	1
TCATGGG	600	0.0	6.000016	1
TCGAGGC	275	0.0	6.000016	1
GTTGGAC	350	0.0	6.000016	1
CGGAGCA	350	0.0	6.000016	1
TCGCGGG	275	0.0	6.000016	1
TTGGGGC	75	1.7513134E-4	6.000016	1
CTGTGCT	175	0.0	6.000016	1
TCACGGC	490	0.0	6.0000157	1
TTTAGCG	565	0.0	6.0000157	1
TTAGGGG	205	0.0	6.0000157	1
GACAGGT	90	9.152125E-6	6.0000157	1
GGGTGCA	70	4.6972596E-4	6.0000157	1
GGATGAT	110	1.8152969E-7	6.0000157	1
CCGTGCA	845	0.0	6.0000157	1
>>END_MODULE