FastQCFastQC Report
Thu 2 Jul 2015
000000000-AGE3A l01n02.3320000000daac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGE3A l01n02.3320000000daac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16049282
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGTCAATCT7962264.961131594547345No Hit
TCTAGCGTAGTG7087894.416328406467031No Hit
AAGAGATGTCGA7003604.363808923040918No Hit
ACGTGTACCCAA6951904.331595643967126No Hit
GACTTTCCCTCG6647094.141674375215041No Hit
ACGCGCAGATAC6075803.7857145260454637No Hit
CTGCTAACGCAA6034313.7598629022781207No Hit
TCCAAAGTGTTC5869213.656992256725254No Hit
TTAGGGCTCGTA5823533.628529924267017No Hit
TCACGGGAGTTG5823013.62820592223378No Hit
GGATGGTGTTGC5417643.3756276449002516No Hit
TCGAGGACTGCA5397553.3631099509622926No Hit
TACAGATGGCTC5380713.3526172697320664No Hit
ATCCCGAATTTG4958203.089359386918368No Hit
ATGTGCACGACT4918593.0646791551173442No Hit
CGGAGCTATGGT4885843.044273257831721No Hit
TAGCTCGTAACT4688432.921270870559817No Hit
TAGGATTGCTCG4161652.5930443492736934No Hit
GCGATATATCGC3830572.3867547470347894No Hit
CAGTGCATATGC3601882.244262391301991No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTC14000.06.0000161
GTGGGCC751.7513134E-46.0000161
TTATGCA751.7513134E-46.0000161
TCTAGAG6050.06.0000161
TTGAGGC751.7513134E-46.0000161
CTGCGAA11000.06.0000161
TCATGGG6000.06.0000161
TCGAGGC2750.06.0000161
GTTGGAC3500.06.0000161
CGGAGCA3500.06.0000161
TCGCGGG2750.06.0000161
TTGGGGC751.7513134E-46.0000161
CTGTGCT1750.06.0000161
TCACGGC4900.06.00001571
TTTAGCG5650.06.00001571
TTAGGGG2050.06.00001571
GACAGGT909.152125E-66.00001571
GGGTGCA704.6972596E-46.00001571
GGATGAT1101.8152969E-76.00001571
CCGTGCA8450.06.00001571
Produced by FastQC (version 0.11.2)