FastQCFastQC Report
Thu 2 Jul 2015
000000000-AGE3A l01n01.3310000000daaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AGE3A l01n01.3310000000daaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16049282
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG316822219.740584033603497No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG5541353.4527089747690893No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG4995463.1125753787614925No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG4462892.780741219451437No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG3679742.292775464970956No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG2580671.6079660136821075No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG1884861.1744201391688425No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1456880.9077540041978203No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG1325580.8259434908053831No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG1129190.7035766459832907No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG1048140.6530759444565807No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG988050.6156350171926694No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG949770.5917834828997335No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG866360.5398123106067922No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG765790.47714907121701766No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG745020.46420768231251713No Hit
TAATCGTCGAGTTTGACATCCAATTATTGCGATTAAAACGTTCGTAGTTG647490.40343860865551495No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG623820.38869028533488287No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG613540.38228501437011325No Hit
AGCTCCAATAGCGTATATTATTGATGTTGCAGTTGAAACGTTCGTAGTCG526280.3279149808695492No Hit
TTCCCACGCCAATGAGGCCGCAACCAAAGACGGTAAGGTGATCGCTGATA480860.2996146494279308No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG475910.2965303993038443No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG460390.28686018477337494No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG457930.2853274059238289No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG452520.28195653861649383No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG419150.2611643312143185No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG413000.2573323840904534No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTTAGCTCGTAACTA385110.23995465965393342No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG383020.23865242071265244No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG327500.20405897285623117No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG327260.20390943345627552No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTTG315810.19677515791672173No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG312090.19445729721740823No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG311670.19419560326748572No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG293210.18269353108755895No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG284280.1771294192475402No Hit
AGCTCCAAAAGCGTATATTAACGCTGCTGCTGTTAAAGGGTTCGTAGTTG267990.16697943247554625No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG264100.16455564803459743No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG240530.1498696327972803No Hit
AGCTCCAATAGCGTAAATCATAGTTGCTGCGATTAAAAAGCTCGTAGTTG231380.14416844317396876No Hit
AGCTCCGCAAGTGTATATCTATATTGCTGAGGTTAAAAAGCTCGTAGTTG228600.14243627845781512No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG224850.14009972533350715No Hit
AGCTCCAATAGCGTATATTAACGTTGTTGCAGTTAAAAAGCTCGTAGTTG197120.12282169383029098No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCCCGTAGTCG179320.11173085500024238No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG176040.10968714986751432No Hit
AGCTCCAATAGCGTATATTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTG170780.10640974468515164No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCA167220.10419157691914194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCA6836150.0476.709631
GCTCCAA6492350.0475.259582
CTCCAAT5571750.0474.15313
AGCTCTC1194400.0466.059051
CTCCAGT408350.0444.83723
GCTCCAG384450.0425.795532
CTCTCTA535200.0419.666323
CTCTCAT480050.0410.91313
AGCTCTA102900.0394.45691
AGCTCCG63550.0389.31581
CTCCAAA702150.0385.622343
GCTCTCA816500.0378.224732
GCTCTCT624650.0360.37372
TCCCACG69750.0345.799352
AACTCCA162900.0320.339051
CCCACGC76400.0315.700323
TAGCGTA9157400.0292.464029
ATAGCGT8509450.0292.21228
AATAGCG8522900.0291.694187
CCAATAG8921400.0291.671635
Produced by FastQC (version 0.11.2)