FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n02 82.3420000000e1bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n02 82.3420000000e1bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895383
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2001.0653068E-525.375145
CGCCGTA7300.022.842464145
TCGGTGG19300.018.03109145
CCGTATC5700.016.53509145
TGGTCGC12800.015.292969145
CGTATCA5250.015.190475145
TCTCGGT21550.013.793504145
GAGTGTA52750.013.056872145
ATCTCGG29250.012.888888145
CTCGGTG21700.012.695852145
GTCGCCG9250.012.540541145
CGGTGGT18650.012.050938145
AGATCTC35000.011.6145
GGTCGCC12050.011.431535145
GGTGGTC19100.011.387435145
GATCTCG29050.011.230637145
TCGCCGT9100.011.153846145
AGAGTGT60200.011.079734145
GTAGATC49900.011.042084145
TAGATCT35350.010.869873145