FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n02 81rr.3420000000e194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n02 81rr.3420000000e194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133723
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA11550.020.086578145
GTCGCCG16250.016.953846145
AGATCTC50150.015.1794615145
TCGGTGG31150.014.662921145
GCGTCGG4703.2824682E-513.882979145
GGTGGTC30750.013.674797145
TCTCGGT35550.013.459916145
GTGGTCG22700.013.414098145
CTCGGTG33500.012.335821145
TAGATCT54300.012.283609145
GATCTCG42750.011.871345145
TGGTCGC20200.011.485149145
GTATCAT12650.011.46245145
CGGTGGT30400.011.447369145
ATCTCGG40700.011.400491145
GGTCGCC18650.011.2734585145
AGTGTAG76350.011.204977145
CCGTATC9500.010.684211140-144
TGTAGAT75450.010.666004145
TCGCCGT14450.010.536332145
Produced by FastQC (version 0.11.2)