FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n02 81r.3420000000e187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n02 81r.3420000000e187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824463
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC6200.023.387096145
GGTCGCC12800.022.65625145
CGCCGTA7400.020.574324145
GCCGTAT6900.017.862318145
CGTATCA5850.017.350428145
TCGCCGT8750.015.742857145
GTCGCCG10150.015.714286145
TCTCGGT27300.015.1373625145
TCGGTGG22700.014.69163145
TGGTCGC13650.014.340659145
CGGTGGT20950.014.188544145
GTGGTCG14900.014.110738145
GGTGGTC21700.014.032259145
AGATCTC41800.013.008373145
CTCGGTG24650.012.941176145
TAGATCT44200.011.809955145
ATCTCGG34700.011.282421145
GTAGATC58850.010.964315145
GTATCAT8605.456968E-1210.959303145
GAGTGTA63350.010.872139145
Produced by FastQC (version 0.11.2)