##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AG3CH l01n02 81r.3420000000e187.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 824463 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.472689496071986 33.0 32.0 34.0 32.0 34.0 2 32.89405952723166 33.0 33.0 34.0 32.0 34.0 3 33.054520336267366 34.0 33.0 34.0 32.0 35.0 4 33.07587241634858 34.0 33.0 34.0 32.0 35.0 5 33.12543194782543 34.0 33.0 34.0 32.0 35.0 6 35.87699993814155 37.0 37.0 37.0 33.0 37.0 7 35.900094970908334 37.0 37.0 37.0 33.0 37.0 8 35.92078965338651 37.0 37.0 37.0 33.0 37.0 9 35.92223180421681 37.0 37.0 37.0 33.0 37.0 10-14 36.538367640512675 37.4 37.4 37.4 34.6 37.4 15-19 37.403423319178664 38.0 38.0 38.0 37.0 38.0 20-24 37.64620073914779 38.4 38.0 38.4 37.0 38.4 25-29 38.065455211452786 39.0 38.0 39.0 37.0 39.0 30-34 38.061203231679286 39.0 38.0 39.0 37.0 39.0 35-39 38.03067499693741 39.0 38.0 39.0 37.0 39.0 40-44 37.93142033056668 39.0 38.0 39.0 37.0 39.0 45-49 37.88204152278513 39.0 38.0 39.0 37.0 39.0 50-54 37.83403742799859 39.0 38.0 39.0 37.0 39.0 55-59 37.843571633899884 39.0 38.0 39.0 37.0 39.0 60-64 37.835913073115464 39.0 38.0 39.0 37.0 39.0 65-69 37.79517140247653 39.0 38.0 39.0 37.0 39.0 70-74 37.74679639959586 39.0 38.0 39.0 37.0 39.0 75-79 37.70474381506508 39.0 38.0 39.0 37.0 39.0 80-84 37.66501322679126 39.0 38.0 39.0 37.0 39.0 85-89 37.66306068313557 39.0 38.0 39.0 37.0 39.0 90-94 37.61677601056687 39.0 38.0 39.0 37.0 39.0 95-99 37.59107200686992 39.0 38.0 39.0 37.0 39.0 100-104 37.53575672892537 39.0 38.0 39.0 36.8 39.0 105-109 37.05192786092281 39.0 37.6 39.0 32.4 39.0 110-114 37.12582104958015 39.0 37.8 39.0 34.8 39.0 115-119 37.18413755377743 39.0 38.0 39.0 35.2 39.0 120-124 37.27921325759919 39.0 38.0 39.0 36.0 39.0 125-129 37.19410428363674 39.0 38.0 39.0 35.6 39.0 130-134 37.117504363446265 39.0 38.0 39.0 35.0 39.0 135-139 36.98049663841798 39.0 38.0 39.0 34.4 39.0 140-144 36.84052504478673 39.0 38.0 39.0 34.0 39.0 145-149 36.734188435381576 39.0 37.8 39.0 34.0 39.0 150-151 35.69138699978046 38.5 36.0 39.0 26.5 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09760923772868324 1101 2 0.055331064458037815 1101 3 0.09397491049442408 1101 4 0.07288608934231888 1101 5 0.13105358616915908 1101 6 0.07655476442425879 1101 7 0.12675530417902792 1101 8 0.14711613670062462 1101 9 0.13073499019377266 1101 10-14 0.09628401900727113 1101 15-19 0.10926715783354979 1101 20-24 0.08400485732420293 1101 25-29 0.13464396095720588 1101 30-34 0.09705125596809694 1101 35-39 0.14428636677224915 1101 40-44 0.14802936320257487 1101 45-49 0.11879354600072389 1101 50-54 0.13952645719167833 1101 55-59 0.14862382458353096 1101 60-64 0.12788970167578384 1101 65-69 0.09354717874978036 1101 70-74 0.02747278011352705 1101 75-79 0.10603252264317575 1101 80-84 0.09925607449269336 1101 85-89 0.1333921340497497 1101 90-94 0.1388225527774054 1101 95-99 0.16494008346299438 1101 100-104 0.10968432398652794 1101 105-109 0.5550496276835588 1101 110-114 0.35788958517112945 1101 115-119 0.3374274684403247 1101 120-124 0.1336905056947444 1101 125-129 0.17928925349567493 1101 130-134 0.13100252118334055 1101 135-139 0.14624912102122778 1101 140-144 0.1634820209720118 1101 145-149 0.12400011639623187 1101 150-151 0.14326154922872547 1102 1 -0.02657908635203654 1102 2 0.04252125757219716 1102 3 0.04793980658503472 1102 4 0.053539609258649534 1102 5 0.057882734621635734 1102 6 0.0440368923137342 1102 7 0.05624609001141323 1102 8 -0.011267535284702035 1102 9 -0.038255030369008125 1102 10-14 0.016853501087368272 1102 15-19 0.01414891457898193 1102 20-24 0.01160711456243746 1102 25-29 -0.0463631485794167 1102 30-34 -0.09572070396406929 1102 35-39 -0.013014448167851356 1102 40-44 -0.04761557939607286 1102 45-49 -0.09507376900292286 1102 50-54 -0.03260823740511398 1102 55-59 -0.029825008662285768 1102 60-64 0.007328751128973465 1102 65-69 0.028268955845831556 1102 70-74 -0.10831878872153311 1102 75-79 -0.017714955585219627 1102 80-84 -0.012587502747948065 1102 85-89 -0.013143404588156216 1102 90-94 -0.06479696138527657 1102 95-99 0.013105613969138119 1102 100-104 0.018386577271670035 1102 105-109 0.45358939546058963 1102 110-114 0.4295712821511728 1102 115-119 0.2969419482613489 1102 120-124 0.046961462055065795 1102 125-129 0.0906140152436663 1102 130-134 0.1013998427285685 1102 135-139 0.16874163750417637 1102 140-144 0.05627725278293383 1102 145-149 0.1888923209198623 1102 150-151 0.19591182984247268 1103 1 -0.02821464208931701 1103 2 0.117534710351471 1103 3 0.05719514094673883 1103 4 0.09076983561756435 1103 5 0.043426621904288254 1103 6 -0.0838742330097304 1103 7 0.04893875586615337 1103 8 -0.014545091204432481 1103 9 -0.09149903628501477 1103 10-14 0.009210944682635613 1103 15-19 0.04458374018285127 1103 20-24 0.04661187400741085 1103 25-29 0.07310537580598009 1103 30-34 0.019716864417887336 1103 35-39 0.1304454122165808 1103 40-44 0.06114325491047623 1103 45-49 -0.05948603171539446 1103 50-54 0.023629724399107488 1103 55-59 -0.015319495939451144 1103 60-64 0.04687479996741928 1103 65-69 -0.05605272018991059 1103 70-74 0.002076534655714113 1103 75-79 0.030934983153656503 1103 80-84 -0.05402317401857459 1103 85-89 -0.051769253324359 1103 90-94 0.028535626762781874 1103 95-99 -0.0040311212682766495 1103 100-104 0.024537600177339414 1103 105-109 0.49983755615483005 1103 110-114 0.4312036378880961 1103 115-119 0.29561983700333627 1103 120-124 0.15943305232968186 1103 125-129 0.20038336077359986 1103 130-134 0.22567156764765173 1103 135-139 0.21935362090551536 1103 140-144 0.12398732697294435 1103 145-149 0.1585418286586986 1103 150-151 0.21330160955244537 2112 1 0.01893641859420825 2112 2 0.02559023081866485 2112 3 -0.0058034020661281716 2112 4 0.003436965672591441 2112 5 0.04370079211759048 2112 6 0.013459966716283134 2112 7 0.09192431630891917 2112 8 0.05776846805552083 2112 9 0.12074633784188649 2112 10-14 0.015620000868878492 2112 15-19 0.05455609894135449 2112 20-24 -0.0447639964830131 2112 25-29 0.06965535992186744 2112 30-34 0.048463910983088 2112 35-39 0.11845942309147262 2112 40-44 0.009772126343726484 2112 45-49 -0.010997086990997218 2112 50-54 0.0070170740744615046 2112 55-59 0.012445666061843497 2112 60-64 -0.050693720940884646 2112 65-69 -0.0016690936794461209 2112 70-74 -0.06926330964667926 2112 75-79 0.04967268573159345 2112 80-84 0.017051846277787774 2112 85-89 0.040162971798913816 2112 90-94 0.015328503487644696 2112 95-99 0.018488354251886108 2112 100-104 -0.023355984483870884 2112 105-109 0.4098472553423633 2112 110-114 0.3298204658119914 2112 115-119 0.17447309955116452 2112 120-124 -0.020330055726020646 2112 125-129 -0.05164890650750209 2112 130-134 0.04673933963837129 2112 135-139 0.008624996358641113 2112 140-144 0.08375789867444183 2112 145-149 0.039650901561557816 2112 150-151 -0.10063044418669165 1113 1 -0.15107115150935613 1113 2 -0.007007275987085393 1113 3 0.011387319594831524 1113 4 0.03706673909907465 1113 5 0.041168300974973704 1113 6 0.05995313070359032 1113 7 0.02746294853673703 1113 8 0.04904972032242938 1113 9 0.03571395907146524 1113 10-14 0.08106134324717118 1113 15-19 0.10057600907028785 1113 20-24 0.10371693161791029 1113 25-29 0.10553644921570537 1113 30-34 0.08875813478491068 1113 35-39 0.04850605866776192 1113 40-44 0.0907066803284522 1113 45-49 0.073798308819498 1113 50-54 0.023528934125891965 1113 55-59 0.03522923008084433 1113 60-64 0.08625519354365707 1113 65-69 0.06545111164347617 1113 70-74 0.14033154072472342 1113 75-79 0.15479801522691616 1113 80-84 0.09381567818321912 1113 85-89 0.08368038637490116 1113 90-94 0.10379764697114524 1113 95-99 0.06469825128333895 1113 100-104 0.11599911923901374 1113 105-109 0.5302504423515231 1113 110-114 0.3596825884583552 1113 115-119 0.28211868163250386 1113 120-124 0.11826469985221877 1113 125-129 0.024099481229548303 1113 130-134 0.10813043183832605 1113 135-139 0.11369273788210421 1113 140-144 0.15898438985356478 1113 145-149 0.10242090080943456 1113 150-151 0.15651075359862432 2113 1 0.04222362166973426 2113 2 0.08457175699571451 2113 3 0.09624765903618737 2113 4 0.070521539584945 2113 5 0.11898061222421319 2113 6 0.05617444892219936 2113 7 0.08460517710565085 2113 8 0.1382914106225286 2113 9 0.24077077929874946 2113 10-14 0.1172614880477525 2113 15-19 0.11899661608605072 2113 20-24 0.1415048161027883 2113 25-29 0.1558117169834432 2113 30-34 0.10025351409975514 2113 35-39 0.13526587237367949 2113 40-44 0.14521489836132417 2113 45-49 0.06972855920717791 2113 50-54 0.07208086166694017 2113 55-59 0.10309536821206677 2113 60-64 0.10026684959027676 2113 65-69 0.09973511544616542 2113 70-74 0.09335695282637602 2113 75-79 0.07825797573342186 2113 80-84 0.09010059665622805 2113 85-89 0.04666810499244178 2113 90-94 0.06178947893847919 2113 95-99 0.01673312608950539 2113 100-104 0.04480283445387556 2113 105-109 0.4849491944798672 2113 110-114 0.16441674072829215 2113 115-119 0.03566889583335353 2113 120-124 -0.10990967951197206 2113 125-129 -0.11172719541048792 2113 130-134 -0.12215702264482076 2113 135-139 -0.05876901863158679 2113 140-144 -0.022401135688539853 2113 145-149 -0.14443648662010844 2113 150-151 -0.07898718839314256 1112 1 0.06701488794355015 1112 2 0.08661298841439447 1112 3 0.06255840624204012 1112 4 0.04713617062348874 1112 5 0.07139276404788575 1112 6 0.050064431997604686 1112 7 0.008368393378674455 1112 8 0.11850516525296229 1112 9 0.035294464024786976 1112 10-14 0.03082891552701028 1112 15-19 0.06230765095707369 1112 20-24 0.09347677845570956 1112 25-29 0.13770941583860719 1112 30-34 0.11656020222658725 1112 35-39 0.07151666874733564 1112 40-44 0.06809222063984066 1112 45-49 0.06898120334419389 1112 50-54 0.07354098298046097 1112 55-59 0.06620081073108963 1112 60-64 0.06003444966863469 1112 65-69 0.11168424989168102 1112 70-74 0.10206553036625365 1112 75-79 0.09789743245217153 1112 80-84 0.16272356469009708 1112 85-89 0.14793502296904393 1112 90-94 0.15024757600693306 1112 95-99 0.1166757695382401 1112 100-104 0.12160387428401975 1112 105-109 0.5542595569556212 1112 110-114 0.38402716620801414 1112 115-119 0.2124508756403216 1112 120-124 0.1583280272142744 1112 125-129 0.1292252057112293 1112 130-134 0.16462441006887474 1112 135-139 0.14590867284664455 1112 140-144 0.18004463589825548 1112 145-149 0.19154632229786728 1112 150-151 0.27137177537692025 2114 1 0.026367582015794255 2114 2 0.01437024299938372 2114 3 0.024707345803292924 2114 4 0.05846487817463242 2114 5 0.008116503531731212 2114 6 0.037790835385543176 2114 7 1.418818407827871E-4 2114 8 0.10641513895544819 2114 9 0.07505664797078282 2114 10-14 0.06042244246773265 2114 15-19 0.07866646973190683 2114 20-24 0.08897233292343287 2114 25-29 0.05244029321933397 2114 30-34 0.05872701499055921 2114 35-39 0.03711616101177384 2114 40-44 0.0446851556018828 2114 45-49 0.02225273941503758 2114 50-54 0.09005661918678243 2114 55-59 0.09568362627280891 2114 60-64 0.07030841057526516 2114 65-69 0.04770103021066774 2114 70-74 0.07849054829637936 2114 75-79 0.06765215974352401 2114 80-84 0.1489751442486167 2114 85-89 0.05765798832690905 2114 90-94 0.11155379975211588 2114 95-99 0.14568727897005118 2114 100-104 0.0527504914673429 2114 105-109 0.4948772639489363 2114 110-114 0.3372241357324981 2114 115-119 0.22123549785546004 2114 120-124 0.06436279457803096 2114 125-129 0.0966447478384751 2114 130-134 0.16125183917374386 2114 135-139 0.13906135781152074 2114 140-144 0.1333221486770313 2114 145-149 0.18795887452291282 2114 150-151 0.25411616580963425 1114 1 0.06451391558652375 1114 2 0.07609883054566069 1114 3 0.08054920126217269 1114 4 0.08361050915790713 1114 5 0.10294531162330145 1114 6 0.17548053072600567 1114 7 0.08927944811448896 1114 8 0.06415478734771796 1114 9 0.10279969372880515 1114 10-14 0.08552522477508973 1114 15-19 0.15974211759733947 1114 20-24 0.10289331167878402 1114 25-29 0.10149454771124056 1114 30-34 0.11557654483264912 1114 35-39 0.09520354876023163 1114 40-44 0.09838385766860114 1114 45-49 0.060468699358395384 1114 50-54 0.10535965854258222 1114 55-59 0.11660832468572835 1114 60-64 0.08435204775405225 1114 65-69 0.13939327161291004 1114 70-74 0.1210396323814038 1114 75-79 0.19135960163178112 1114 80-84 0.21279145448262682 1114 85-89 0.18141257466164262 1114 90-94 0.1425745402557297 1114 95-99 0.17169330700464513 1114 100-104 0.09958165198393232 1114 105-109 0.5841592727820881 1114 110-114 0.3890267457292964 1114 115-119 0.2795605049533023 1114 120-124 0.1307940297581709 1114 125-129 0.16054736599070196 1114 130-134 0.20837539556586648 1114 135-139 0.2631455845706441 1114 140-144 0.14632679813997385 1114 145-149 0.17439876230081097 1114 150-151 0.25162767171489975 1109 1 -0.17018166962083825 1109 2 -0.10518226546653864 1109 3 0.02824072187534199 1109 4 0.06471739587324521 1109 5 0.03145438305259063 1109 6 -0.04467435188783497 1109 7 -0.01718303090160589 1109 8 7.630933934734685E-4 1109 9 0.07437206178143185 1109 10-14 0.06654891385338857 1109 15-19 0.061657312876285175 1109 20-24 0.06947245405505242 1109 25-29 0.07876038640984007 1109 30-34 0.12426586840308573 1109 35-39 0.006015095802169412 1109 40-44 0.07353977998158712 1109 45-49 0.01678084525835999 1109 50-54 0.06780014716211724 1109 55-59 0.09284438370938375 1109 60-64 0.05370050151088179 1109 65-69 0.13786945034739517 1109 70-74 0.08297928555165868 1109 75-79 0.06607283784838813 1109 80-84 0.10898138038398031 1109 85-89 0.15545475996599123 1109 90-94 0.04279559027806812 1109 95-99 0.10603971392161782 1109 100-104 -0.06767887317916887 1109 105-109 0.42158021549184355 1109 110-114 0.4149052915049296 1109 115-119 0.28271637169989816 1109 120-124 0.02770393103667601 1109 125-129 -0.07061118009747958 1109 130-134 0.02938795400282146 1109 135-139 0.02102700330198104 1109 140-144 -0.10607748984844534 1109 145-149 0.06947063177537416 1109 150-151 -0.056435184284374884 1108 1 0.3394063822482849 1108 2 0.28169949565329944 1108 3 0.2627114079801345 1108 4 0.27331048759313603 1108 5 0.24022297598155262 1108 6 0.1329436865954392 1108 7 0.10853483489661642 1108 8 0.09487189579099464 1108 9 0.09550705454883257 1108 10-14 0.11566877903761963 1108 15-19 0.1442635797150018 1108 20-24 0.11109388820296573 1108 25-29 0.15923361219595478 1108 30-34 0.15399744364047052 1108 35-39 0.08952919323928654 1108 40-44 0.08216094761179704 1108 45-49 0.09644072627089884 1108 50-54 0.11524263196798046 1108 55-59 0.14957757486425294 1108 60-64 0.16996128281571288 1108 65-69 0.16442821214621262 1108 70-74 0.1954846622169768 1108 75-79 0.11266833390271103 1108 80-84 0.1706657615366325 1108 85-89 0.13007392287768482 1108 90-94 0.12790046322600546 1108 95-99 0.16365726412819015 1108 100-104 0.08064551858706892 1108 105-109 0.4112549407585675 1108 110-114 0.5396574616325225 1108 115-119 0.4206083034306758 1108 120-124 0.22547351304719143 1108 125-129 0.1754048942713382 1108 130-134 0.25795918677406604 1108 135-139 0.3014329625477927 1108 140-144 0.22816635103888672 1108 145-149 0.2503898919033958 1108 150-151 0.3016581680103698 1111 1 0.286718975385007 1111 2 0.08357310199000523 1111 3 0.06570600262298854 1111 4 0.1826606013179699 1111 5 0.10719010322264921 1111 6 -0.011525116070451702 1111 7 -0.0659430415255855 1111 8 0.08935080520583227 1111 9 0.060960146161889384 1111 10-14 0.007318946516335245 1111 15-19 -0.02915847620815981 1111 20-24 -0.0024336604991290756 1111 25-29 -0.0688390467207256 1111 30-34 -0.014982680178363239 1111 35-39 -0.014311945246134883 1111 40-44 -0.0749455634938343 1111 45-49 -0.04420625869778405 1111 50-54 -0.10573311247218697 1111 55-59 -0.004681511166822361 1111 60-64 -0.050954777605426216 1111 65-69 -0.053614024499644586 1111 70-74 -0.07500885958012304 1111 75-79 -0.1332894447550217 1111 80-84 -0.07957153982749077 1111 85-89 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120-124 -0.3588665269073843 2110 125-129 -0.15806084809599952 2110 130-134 -0.2761228963484328 2110 135-139 -0.18818253735761914 2110 140-144 -0.39946049626946944 2110 145-149 -0.2665611732714339 2110 150-151 -0.2172238863963898 2109 1 -0.006867605245069797 2109 2 -0.06849617367383587 2109 3 -0.1706767406369707 2109 4 -0.1493760718452819 2109 5 -0.1386060474503239 2109 6 -0.009145691516543764 2109 7 -0.15178965034738923 2109 8 -0.16990550173063212 2109 9 -0.19565169144205186 2109 10-14 -0.1510739388581115 2109 15-19 -0.18723437068447168 2109 20-24 -0.1599495731499445 2109 25-29 -0.20536301434141535 2109 30-34 -0.15130311466580082 2109 35-39 -0.12793657541234182 2109 40-44 -0.14901050012886685 2109 45-49 -0.16559929154075093 2109 50-54 -0.1334257083566186 2109 55-59 -0.20714091414515678 2109 60-64 -0.09007751260183738 2109 65-69 -0.19939303693882238 2109 70-74 -0.14798399054147637 2109 75-79 -0.19784372446427767 2109 80-84 -0.151645917105121 2109 85-89 -0.15602237284347353 2109 90-94 -0.17702169708016413 2109 95-99 -0.13240539252838346 2109 100-104 -0.08350389221499199 2109 105-109 0.2164358477856112 2109 110-114 0.20417564789698872 2109 115-119 0.035177820956633354 2109 120-124 -0.04963457459621168 2109 125-129 -0.07746876048129536 2109 130-134 -0.010886420603831937 2109 135-139 -0.014521735871916519 2109 140-144 -0.033894239094763634 2109 145-149 -0.07768323141971223 2109 150-151 0.08323419368993257 2108 1 0.07455881434498224 2108 2 0.04132320901578623 2108 3 -0.07983666666366673 2108 4 -0.03765808427813511 2108 5 -0.12290159392151878 2108 6 0.002130078459657625 2108 7 -0.0860876720471282 2108 8 -0.14423505760955635 2108 9 -0.04625735546219545 2108 10-14 -0.10800882035973558 2108 15-19 -0.13643703333312374 2108 20-24 -0.172070407067217 2108 25-29 -0.14923087385906086 2108 30-34 -0.04803072308604328 2108 35-39 -0.09271270203063153 2108 40-44 -0.13506001910945287 2108 45-49 -0.16222651956601197 2108 50-54 -0.10232231190148156 2108 55-59 -0.2194555131360474 2108 60-64 -0.231435374171717 2108 65-69 -0.2571385615977917 2108 70-74 -0.16321719774273902 2108 75-79 -0.21105291520864 2108 80-84 -0.2342275130720637 2108 85-89 -0.225628611627954 2108 90-94 -0.12229534695919853 2108 95-99 -0.12846701340099997 2108 100-104 -0.15097650723932787 2108 105-109 0.3070510594478648 2108 110-114 0.2346896378189669 2108 115-119 0.0705600857233506 2108 120-124 -0.042241706089882314 2108 125-129 -0.07086987091762609 2108 130-134 -0.08580947353444657 2108 135-139 -0.1331330373703281 2108 140-144 -0.05735485030655241 2108 145-149 -0.2119323063466183 2108 150-151 -0.06847790910850904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 8.0 15 46.0 16 203.0 17 408.0 18 742.0 19 1031.0 20 1074.0 21 1165.0 22 1346.0 23 1614.0 24 1923.0 25 2334.0 26 2899.0 27 3576.0 28 4360.0 29 5389.0 30 6429.0 31 7995.0 32 10005.0 33 12457.0 34 17039.0 35 25149.0 36 45185.0 37 141793.0 38 530293.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.950957168484212 30.765480076122277 30.366311162538523 20.917251592854985 2 20.804450897129403 31.0986666472601 29.409567197072516 18.687315258537982 3 20.94369304626163 30.10177533740144 29.386400602574035 19.568131013762898 4 21.60303130643825 29.05503339749631 29.17875028958243 20.16318500648301 5 21.8189294122356 29.596112863767083 29.180933528854542 19.404024195142778 6 21.700064162976386 29.73402081112191 28.50728292233854 20.058632103563166 7 21.80849838015775 29.56724558894699 29.070801236683756 19.553454794211504 8 20.940296896282792 29.73899374501949 29.212590498300106 20.108118860397617 9 20.668726189046687 29.403381352468212 29.756823532408365 20.17106892607673 10-14 20.601870550891913 29.57105412856611 29.635726527448774 20.1913487930932 15-19 20.584380378500917 29.282332863936887 29.714323141244662 20.41896361631753 20-24 20.949927407294204 29.040987891512415 29.311248655185253 20.697836046008128 25-29 21.11789128196171 28.928842167568465 29.174941749963306 20.77832480050651 30-34 21.23382128731065 28.895826738131365 28.984636060077897 20.88571591448009 35-39 21.295000503357944 28.767573560002084 28.951402306713582 20.98602362992639 40-44 21.365033967564344 28.77788330100926 28.85512145481362 21.001961276612775 45-49 21.418110940090703 28.71660705210543 28.79124957699739 21.074032430806476 50-54 21.39065064169041 28.725582591335208 28.771382099621206 21.112384667353172 55-59 21.369909868605383 28.74101081552477 28.746323364420235 21.142755951449608 60-64 21.29665006191909 28.750835392249257 28.715297108542163 21.237217437289484 65-69 21.328185740293986 28.694629110099545 28.733854642355084 21.243330507251386 70-74 21.362972019362907 28.730822365588267 28.645845841474998 21.26035977357383 75-79 21.342692152346437 28.721385920289933 28.670370895965007 21.26555103139862 80-84 21.325420303882648 28.804421787272442 28.70028127399289 21.16987663485202 85-89 21.26967492780149 28.77480250781418 28.689146753705142 21.266375810679193 90-94 21.306911286498 28.73720227590565 28.692130514043683 21.26375592355266 95-99 21.32622082494909 28.876080551825854 28.581367508305405 21.21633111491965 100-104 21.416558414386092 28.93493097931623 28.481278116786317 21.167232489511353 105-109 21.56705637487674 28.957297052748277 28.415441323625195 21.060205248749792 110-114 21.900097396729752 29.140106954466123 28.140814081408138 20.818981567395987 115-119 22.3854557451335 29.284443328566596 27.701473565217604 20.628627361082305 120-124 22.932211633511752 29.39656479429641 27.332117996805195 20.339105575386647 125-129 23.782583329997827 29.494786303327135 26.761297960005482 19.961332406669555 130-134 24.724893658053787 29.506478762539977 26.21716195875376 19.551465620652472 135-139 25.783012700388007 29.45912672854937 25.75742028447608 19.000440286586542 140-144 26.956139935934058 29.363064200576616 25.26485724647437 18.415938617014955 145-149 28.059112416203032 29.28199324893901 24.81212619608157 17.84676813877639 150-151 28.943081739265438 29.11840798192278 24.446033357470256 17.492476921341527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13.0 1 12.5 2 11.0 3 11.0 4 16.0 5 26.5 6 33.0 7 34.0 8 41.5 9 50.5 10 62.5 11 84.0 12 121.0 13 176.0 14 240.5 15 313.0 16 459.0 17 679.0 18 979.0 19 1372.5 20 1980.0 21 2799.5 22 3836.5 23 5090.0 24 6469.5 25 8213.0 26 10218.0 27 12159.5 28 14128.0 29 16330.0 30 18569.5 31 20691.0 32 22633.5 33 24156.0 34 25392.5 35 26700.5 36 27891.5 37 28755.5 38 29306.5 39 29470.5 40 29109.0 41 28515.0 42 27939.5 43 27318.5 44 26526.0 45 26085.5 46 25837.5 47 25403.5 48 24788.5 49 23632.5 50 22610.0 51 21403.0 52 19979.5 53 18823.5 54 17720.0 55 16681.0 56 15438.0 57 14154.5 58 12977.5 59 11413.0 60 9638.0 61 8075.5 62 6618.0 63 5354.5 64 4299.5 65 3395.5 66 2693.0 67 2073.5 68 1593.5 69 1224.0 70 905.5 71 670.5 72 508.0 73 381.5 74 266.0 75 202.0 76 165.5 77 127.5 78 98.0 79 79.5 80 57.5 81 38.5 82 23.5 83 25.5 84 22.0 85 9.5 86 5.5 87 5.0 88 4.0 89 3.5 90 3.5 91 4.0 92 3.0 93 0.5 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 824463.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.5067503146185 #Duplication Level Percentage of deduplicated Percentage of total 1 87.89418855974168 76.91334812403313 2 10.391824935318676 18.18709659856316 3 1.3779274627720217 3.617338633093413 4 0.2532865985627175 0.8865714855386693 5 0.05817537002584815 0.25453687896562177 6 0.014614390702626242 0.0767314702928998 7 0.006293641921330188 0.03855153065256197 8 0.0036890409550843226 0.02582527886055725 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.2129107067266814E-4 0.0 0.0 2 1.2129107067266814E-4 0.0 0.0 3 1.2129107067266814E-4 0.0 0.0 4 1.2129107067266814E-4 0.0 0.0 5 1.2129107067266814E-4 0.0 1.2129107067266814E-4 6 2.425821413453363E-4 0.0 1.2129107067266814E-4 7 2.425821413453363E-4 0.0 1.2129107067266814E-4 8 3.6387321201800446E-4 0.0 1.2129107067266814E-4 9 4.851642826906726E-4 0.0 1.2129107067266814E-4 10-11 4.851642826906726E-4 0.0 1.2129107067266814E-4 12-13 7.277464240360089E-4 0.0 1.2129107067266814E-4 14-15 0.0010309741007176792 0.0 1.2129107067266814E-4 16-17 0.0012735562420630156 0.0 4.851642826906726E-4 18-19 0.0019406571307626903 0.0 4.851642826906726E-4 20-21 0.002547112484126031 0.0 4.851642826906726E-4 22-23 0.0033355044434983743 0.0 4.851642826906726E-4 24-25 0.004063250867534383 6.064553533633407E-5 4.851642826906726E-4 26-27 0.004730351756234058 1.2129107067266814E-4 6.064553533633408E-4 28-29 0.005276161574261065 1.2129107067266814E-4 6.064553533633408E-4 30-31 0.005579389250942735 1.2129107067266814E-4 6.064553533633408E-4 32-33 0.006185844604306076 1.2129107067266814E-4 7.277464240360089E-4 34-35 0.0066103633516604145 1.2129107067266814E-4 7.277464240360089E-4 36-37 0.006974236563678418 1.2129107067266814E-4 7.277464240360089E-4 38-39 0.007398755311032757 2.425821413453363E-4 7.277464240360089E-4 40-41 0.007641337452378093 2.425821413453363E-4 7.277464240360089E-4 42-43 0.008065856199732432 2.425821413453363E-4 7.277464240360089E-4 44-45 0.00879360262376844 2.425821413453363E-4 7.277464240360089E-4 46-47 0.009460703512468116 2.425821413453363E-4 7.277464240360089E-4 48-49 0.010370386542513127 2.425821413453363E-4 7.277464240360089E-4 50-51 0.010976841895876468 2.425821413453363E-4 7.277464240360089E-4 52-53 0.011765233855248811 2.425821413453363E-4 7.277464240360089E-4 54-55 0.01279620795596649 2.425821413453363E-4 7.277464240360089E-4 56-57 0.013887827592020504 2.425821413453363E-4 7.277464240360089E-4 58-59 0.01564654811677419 2.425821413453363E-4 7.277464240360089E-4 60-61 0.016798813288164537 2.425821413453363E-4 7.88391959372343E-4 62-63 0.01801172399489122 2.425821413453363E-4 8.490374947086771E-4 64-65 0.020194963266999245 2.425821413453363E-4 8.490374947086771E-4 66-67 0.022560139145116277 2.425821413453363E-4 9.703285653813452E-4 68-69 0.025653061447269317 2.425821413453363E-4 0.0010916196360540133 70-71 0.02953437570879469 2.425821413453363E-4 0.0010916196360540133 72-73 0.03468924621238309 2.425821413453363E-4 0.0010916196360540133 74-75 0.04123896402870717 2.425821413453363E-4 0.0010916196360540133 76-77 0.04985063004646661 2.425821413453363E-4 0.0010916196360540133 78-79 0.06313200228512378 2.425821413453363E-4 0.0010916196360540133 80-81 0.08363019322880469 2.425821413453363E-4 0.0011522651713903474 82-83 0.10758517968665665 4.245187473543385E-4 0.0012129107067266815 84-85 0.14403314642379345 6.064553533633408E-4 0.0012129107067266815 86-87 0.19066956309743432 6.064553533633408E-4 0.0012129107067266815 88-89 0.2566519055433658 6.064553533633408E-4 0.0012129107067266815 90-91 0.33840208717674414 6.064553533633408E-4 0.0012129107067266815 92-93 0.44556274811604646 6.064553533633408E-4 0.0013948473127356838 94-95 0.5825003669054888 6.064553533633408E-4 0.001576783918744686 96-97 0.7692886157413977 6.064553533633408E-4 0.001576783918744686 98-99 0.9933738688091522 6.064553533633408E-4 0.001576783918744686 100-101 1.2512386850592447 6.064553533633408E-4 0.001576783918744686 102-103 1.5672019241615451 6.064553533633408E-4 0.001576783918744686 104-105 1.9649759904325603 7.277464240360089E-4 0.001576783918744686 106-107 2.458145483787629 7.277464240360089E-4 0.001576783918744686 108-109 3.03415677841213 7.277464240360089E-4 0.001576783918744686 110-111 3.732672054416026 7.277464240360089E-4 0.001576783918744686 112-113 4.519365938798952 7.277464240360089E-4 0.001576783918744686 114-115 5.434992231306923 7.277464240360089E-4 0.001576783918744686 116-117 6.501019451449004 7.277464240360089E-4 0.0018193660600900221 118-119 7.710594653732187 7.277464240360089E-4 0.0018193660600900221 120-121 9.028058263378707 7.88391959372343E-4 0.0018193660600900221 122-123 10.45122704111646 8.490374947086771E-4 0.0018193660600900221 124-125 12.003510163585268 8.490374947086771E-4 0.0018193660600900221 126-127 13.692791550378853 8.490374947086771E-4 0.0018193660600900221 128-129 15.502818198027079 8.490374947086771E-4 0.0018193660600900221 130-131 17.43947272345757 8.490374947086771E-4 0.0019406571307626903 132-133 19.467156197427904 8.490374947086771E-4 0.0019406571307626903 134-135 21.5770750173143 8.490374947086771E-4 0.0019406571307626903 136-137 23.745880652012282 8.490374947086771E-4 0.0019406571307626903 138-139 26.017116595893327 8.490374947086771E-4 0.0019406571307626903 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTATC 620 0.0 23.387096 145 GGTCGCC 1280 0.0 22.65625 145 CGCCGTA 740 0.0 20.574324 145 GCCGTAT 690 0.0 17.862318 145 CGTATCA 585 0.0 17.350428 145 TCGCCGT 875 0.0 15.742857 145 GTCGCCG 1015 0.0 15.714286 145 TCTCGGT 2730 0.0 15.1373625 145 TCGGTGG 2270 0.0 14.69163 145 TGGTCGC 1365 0.0 14.340659 145 CGGTGGT 2095 0.0 14.188544 145 GTGGTCG 1490 0.0 14.110738 145 GGTGGTC 2170 0.0 14.032259 145 AGATCTC 4180 0.0 13.008373 145 CTCGGTG 2465 0.0 12.941176 145 TAGATCT 4420 0.0 11.809955 145 ATCTCGG 3470 0.0 11.282421 145 GTAGATC 5885 0.0 10.964315 145 GTATCAT 860 5.456968E-12 10.959303 145 GAGTGTA 6335 0.0 10.872139 145 >>END_MODULE