FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n02 80rr.3420000000e16d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n02 80rr.3420000000e16d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272983
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGA1258.913048E-1152.22
GATCCGG1650.048.3333321
GATTGGG6400.040.781251
GAGAGGG17400.036.6666647
GTCGGGA2900.035.0000042
TCCGGAG2950.034.406783
GGTCCGG1905.4296834E-934.3421061
ATCCGGA2455.4569682E-1132.551022
GGTCGGG3450.029.4202881
CCGGAGA4300.028.662794
TTGGGAG11050.024.9321253
GGAGAGG26200.024.3511436
TGGGAGA13850.024.0794224
AGAGGGG28700.023.9982578
CGGGAGA7650.023.692814
GATCGGG3402.5065674E-923.4558811
TCGGAGA5350.023.0373824
GAGGGGC18750.022.8133349
GATTCGG2903.287514E-722.5000021
ATTGGGA12400.022.2177412
Produced by FastQC (version 0.11.2)