FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n02 80rr-wga.3420000000e144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n02 80rr-wga.3420000000e144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984564
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT7454.5838533E-1014.597315145
TAGATCT16150.012.569659145
GTCGCCG8700.012.499999145
CTCGGTG13900.011.996403145
TCGGTGG14250.011.701755145
CCGTATC5604.9695336E-711.651786145
ATCTCGG14400.09.565972145
TGTAGGG47100.09.389597145
GAGTGTA26300.09.372623145
TCTCGGT12750.09.098039145
GGTGGTC15152.3137545E-99.092409145
GATCTCG13950.09.043011140-144
AGAGTGT27350.09.012797145
AGATCTC15600.08.923077140-144
CGTGTAG49950.07.837837145
GTAGATC23200.07.8125145
CGCCGTA6600.07.6893945140-144
AAGAGTG31200.07.668269145
GGTCGCC10400.07.5288463140-144
AGTGTAG24400.07.4877043140-144
Produced by FastQC (version 0.11.2)