FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n02 80r.3420000000e137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n02 80r.3420000000e137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011696
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATA1500.001494285124.1666681
GCGTCGG4202.8567229E-818.988094145
CCGTATC7150.014.195805145
TAGATCT29700.013.181818145
GGTCGCC13850.013.086642145
GTCGTGG4000.002137165512.6875145
ATCTCGG24350.012.207393145
CTCGGTG21650.012.055428145
TCTCGGT20300.011.071428145
AAAGAGT62250.010.947792145
AGTGTAG44050.010.698071145
TCGCCGT12350.010.566801145
TGTAGGG89500.010.530726145
TGGTCGC14500.010.5145
GTGGTCG15950.010.454545145
GAGTGTA45950.010.2557125145
GATCTCG24250.010.164948145
AGATCTC27950.09.597495145
CGCCGTA8600.09.273255145
TAGGGAA82050.09.189518145