FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n02 80.3420000000e12a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n02 80.3420000000e12a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270195
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA8800.018.125145
GGTCGCC18300.016.24317145
TCTCGGT34000.015.992648145
CTCGGTG32700.014.189602145
CGCCGTA11450.013.930131145
TCGCCGT15650.013.897764145
TCTCGGG4302.4049885E-413.488373145
GCGTCGG7851.4942998E-812.929935145
TCGGTGG28800.012.838543145
GAGTGTA80200.012.65586145
TAGATCT50600.012.465416145
GTGGTCG21750.012.333333145
GCCGTAT10350.011.908213145
GATCTCG42150.011.868327145
TGTAGAT74100.011.838732145
CCGTATC8450.011.668639140-144
AGATCTC47450.011.612224145
ATCTCGG40700.011.222359145
AGTGTAG73700.011.214382145
GTAGATC75450.011.0503645145
Produced by FastQC (version 0.11.2)