FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n02 130.3420000000e1e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n02 130.3420000000e1e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918259
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2603.6379788E-1233.46154145
CGTATCA6700.017.313433145
GATCTCG27600.015.76087145
CGCCGTA8900.015.477528145
CCGTATC7650.015.163399145
TCGCCGT10450.013.181818145
CTCGGTG20500.012.731708145
GAGTGTA48000.012.083334145
GTGGTCG14100.011.8262415145
ATCTCGG26500.011.764152145
TCTCGGT21500.011.127907145
GGTCGCC12600.010.93254145
GTCGCCG10750.010.925581140-144
GTGTAGA43250.010.728323145
TGTAGAT44800.010.518973145
TAGATCT33350.010.217392145
GGTGGTC19500.010.038462145
AGAGCGG5800.002952895410.0145
GCCGTAT8350.09.898205140-144
CGGTGGT18250.09.136986145