FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 82r.3410000000e1cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 82r.3410000000e1cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802838
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7800.023.23718145
GCCGTCT6900.023.115942145
ATGCCGT9250.020.378378145
ATATCGT2307.960075E-418.913042145
TCGTATG15000.018.366667145
TATGCCG10050.017.313433145
GATATCG1700.007665734717.058823145
ATCTCGT20150.016.191067145
CGTATGC13000.016.173077145
CTCGTAT15600.015.336538145
TCTCGTA17700.015.155367145
GATCTCG25750.014.359224145
GTATGCC12950.013.996139145
TAGCCGG3700.001187696913.7162151
CGTCTTC5900.013.516949145
ACGATCA45500.013.225274145
AGATCTC31400.011.775478145
GTCACGA58350.011.67952145
CACGATC50950.011.668303145
TCACGAT54100.011.658965145