FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 82.3410000000e1b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 82.3410000000e1b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895383
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9100.016.730768145
TATCTCG15000.015.949999145
CTCGTAT11900.015.840337145
ATCTCGT14650.014.8464155145
TTATCTC23900.013.043933145
TGCCGTC7900.012.848102145
CAGTCAC54450.012.51607145
CACTAGC38050.012.38502145
TAGCTTA30150.012.263681145
GCTTATC26300.012.129278145
AGCTTAT28900.012.041522145
GCCGTCT6650.011.992481145
CCAGTCA60000.011.962499145
CTAGCTT33450.011.704037145
CTTATCT25250.011.198019145
TCTCGTA12600.011.162698140-144
AACTCCA82200.011.113138145
ACTAGCT34800.011.041666145
TCACTAG41500.010.132529145
GTCACTA44450.09.949382145