FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 81rr.3410000000e197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 81rr.3410000000e197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133723
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13400.017.854477145
ATCTCGT24350.017.268993145
TCTCGTA21250.015.0117655145
CTTAGGC47950.014.817519145
CCGTCTT10950.014.56621145
CGTATGC21300.013.615024145
CTCGTAT18400.013.002718145
GCATCTC35200.012.975852145
ACTTAGG53850.012.790157145
CATCTCG27950.012.450805145
TCACTTA67350.012.164067145
TTAGGCA45300.012.00331145
AGGCATC39050.011.696544145
CACTTAG60500.011.623967145
AGTCACT79450.011.22404145
CTCCAGT106900.011.122544145
TCGTATG19650.011.068703145
GGCATCT34550.010.701881145
TCCAGTC97050.010.682637145
CCAGTCA90300.010.43743145
Produced by FastQC (version 0.11.2)