FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 81r.3410000000e18a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 81r.3410000000e18a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824463
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6750.021.481482145
TCGTATG12950.021.274132145
ATGCCGT7650.017.058823145
CTCGTAT13200.017.026514145
GCCGTCT5700.016.535088145
GATATCG1851.9672365E-415.675674145
TATGCCG8350.015.628743145
CACGATC26150.015.525812145
ATCTCGT16200.015.216049145
TCTCGTA14600.014.897261145
CGTATGC11700.014.871795145
CATCACG37300.014.383379145
CGATATC2108.716879E-613.809524145
CCGTCTT6450.013.488372145
ACGATCT23350.013.040685145
GATCTCG21850.012.940503145
GTATGCC12350.012.91498145
TCACGAT29100.012.457045145
ACATCAC48950.011.700716145
GTCACAT57650.011.318299145
Produced by FastQC (version 0.11.2)