FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 81.3410000000e17d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 81.3410000000e17d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013272
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG6201.553235E-714.032258145
ACGTCTG33550.010.588674145
GAACTCC30000.010.150001145
CTCGTAT5101.1541388E-49.95098145
GTCACCT15654.1836756E-119.728435145
CGTCTGA28350.09.7178135145
AGTCACC15750.09.666667145
CACGTCT36450.09.547325145
CAGTCAC16350.09.311926145
ACACGTC37500.09.28145
CAGATCG18800.09.25532145
CTGAACT26000.09.201923145
GATCGGA72300.08.724066145
CTTGTAA10950.08.607306145
TTGTAAT13904.8140464E-78.345324145
GTCTGAA27850.08.330341145
AACTCCA25300.08.310276145
AGCACAC50950.08.110892145
TCGGAAG67200.08.091517145
TCTGAAC27400.07.885037140-144
Produced by FastQC (version 0.11.2)