FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 80rr.3410000000e160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 80rr.3410000000e160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272983
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC102930.8085732488179339TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA25700.016.13615655-59
TGCTTGA26650.015.615355555-59
CTTGAAA29950.013.84638460-64
CTGCTTG31050.013.72944355-59
GCCGTCT29550.013.6903345-49
TGCCGTC31750.012.78737745-49
CCGTCTT31950.012.75271450-54
GTCTTCT34700.012.20170650-54
ATGCCGT34400.012.01305945-49
TATGCCG36250.011.51997945-49
GAGCACA182000.011.4326719
AGAGCAC182900.011.3764138
CGTCTTC37050.011.19296150-54
AAGAGCA197100.010.630377
TCTTCTG39850.010.55205150-54
GAAGAGC204750.010.3040096
TCTGCTT42200.010.17059655-59
CGGAAGA209950.09.9452074
TCGGAAG210350.09.8918283
ATCGGAA215200.09.7025832
Produced by FastQC (version 0.11.2)