FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 80rr-wga.3410000000e147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 80rr-wga.3410000000e147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984564
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTC12350.012.914981145
ACCGATG18600.010.524194145
TGTATCT15850.010.063092145
CAGTCAC25450.09.97053145
CCAGTCA26400.09.886364145
GTCTGAA44600.09.590807145
TGAACTC38800.09.52964145
ATCTCGT10800.09.398149145
ATGCCGT8507.824334E-59.382353145
TGCCGTC7204.304693E-79.0625145
TCCAGTC27500.08.963636145
TCACCGA22150.08.837472145
GTCACCG22500.08.700001145
CACCGAT20900.08.672249145
CCGATGT16800.08.630952145
CAAGATC7700.00314658028.474026145
CTGAACT40450.08.244746145
AGTCACC24300.07.63786140-144
TATCTCG9900.07.616162140-144
CGTCTGA46300.07.516199145