FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 80r.3410000000e13a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 80r.3410000000e13a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011696
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAGT59550.012.418137145
CTCGTAT13800.012.083334145
ACCAGAT35000.011.392858145
AGATCAT26450.011.238185145
AGTCACC43850.011.077538145
TCGTATG16400.011.051829145
GTCTGAA89950.010.800445145
ATCTCGT15750.010.587301145
CAGTCAC45250.010.574586145
ATCATCT24850.010.211268145
TCCAGTC50500.010.193069145
CATCTCG17800.010.182585145
GATCATC23050.09.750543145
TGCCGTC9853.861209E-69.568528145
AACTCCA72500.09.5145
CTGAACT80600.09.354838145
ACTCCAG66200.09.089879145
CGTCTGA95300.08.976914145
CCAGATC39900.08.903508145
TCATCTC23200.08.750001145